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Protein

Cytochrome b-245 light chain

Gene

CYBA

Organism
Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron (heme axial ligand)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 light chain
Alternative name(s):
Cytochrome b(558) alpha chain
Cytochrome b558 subunit alpha
Neutrophil cytochrome b 22 kDa polypeptide
Superoxide-generating NADPH oxidase light chain subunit
p22 phagocyte B-cytochrome
p22-phox
Short name:
p22phox
Gene namesi
Name:CYBA
OrganismiTursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus)
Taxonomic identifieri9739 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaCetaceaOdontocetiDelphinidaeTursiops

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Intramembranei91 – 127By similarityAdd BLAST37

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001449121 – 192Cytochrome b-245 light chainAdd BLAST192

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphothreonineBy similarity1
Modified residuei168PhosphoserineBy similarity1

Post-translational modificationi

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.By similarity
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-1523302.

Family & Domainsi

Sequence similaritiesi

Belongs to the p22phox family.Curated

Phylogenomic databases

eggNOGiENOG410IXYN. Eukaryota.
ENOG4111MI6. LUCA.
HOGENOMiHOG000001585.
HOVERGENiHBG051278.
OMAiEQWTPIE.
OrthoDBiEOG091G0NNC.
TreeFamiTF328901.

Family and domain databases

InterProiView protein in InterPro
IPR007732. Cyt_b558_asu.
PANTHERiPTHR15168. PTHR15168. 1 hit.
PfamiView protein in Pfam
PF05038. Cytochrom_B558a. 1 hit.
PIRSFiPIRSF019635. Cytochr_b558a. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9N2H0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQIEWAMWA NEQALASGLI LITGGIVATA GQFAQWYLGA YSIAAGVLIC
60 70 80 90 100
LLEYPRGKRS KGSTMERCGQ KYLTRAVKVF GPLTSNYYIR AFLHLGLSVP
110 120 130 140 150
AGFLLATILG TACLAIASSI YLLAAIHGEH WTPIETKPKE RPQVGGTIKQ
160 170 180 190
PPSNPPPRPP AEARKKPSEE EVAGVPGGGP QENPMPVTDE VV
Length:192
Mass (Da):20,509
Last modified:January 23, 2007 - v3
Checksum:iDDEEE8EF54518B21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB034193 mRNA. Translation: BAA95155.1.
RefSeqiNP_001267539.1. NM_001280610.1.

Genome annotation databases

EnsembliENSTTRT00000006248; ENSTTRP00000005912; ENSTTRG00000006256.
GeneIDi101328418.

Similar proteinsi

Entry informationi

Entry nameiCY24A_TURTR
AccessioniPrimary (citable) accession number: Q9N2H0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 84 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families