Reviewed, UniProtKB/Swiss-Prot Q9N2D4 (PEPA_CALJA)

Names and origin

Protein names

Recommended name:
    Pepsin A
    EC=3.4.23.1

Gene names

Name:

PGA

Organism

Callithrix jacchus (Common marmoset)

Taxonomic identifier

9483 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. Ref.1
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Enzyme regulation	Inhibited by pepstatin. Ref.1
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase A1 family.
Biophysicochemical properties	pH dependence: Optimum pH is about 2.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Phosphoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Ref.1
	Propeptide	16 – 61	46	Activation peptide By similarity		PRO_0000026009
	Chain	62 – 387	326	Pepsin A		PRO_0000026010
Sites
	Active site	93	1	By similarity
	Active site	276	1	By similarity
Amino acid modifications
	Modified residue	129	1	Phosphoserine By similarity
	Disulfide bond	106 ↔ 111		By similarity
	Disulfide bond	267 ↔ 271		By similarity
	Disulfide bond	310 ↔ 343		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9N2D4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7A7968AA568464BD	FASTA	387	41,564
	10 20 30 40 50 60 MKWLLLLSLV ALSECLYKVS LIKKKSLRKN LIEHGLLKDF LKNNTLDPAS KYFPQGEAAT 70 80 90 100 110 120 MIANQPLVNY LDMEYFGTIG IGTPAQEFTV IFDTGSSNLW VPSIYCSSPA CTNHNRFNPQ 130 140 150 160 170 180 ESSTYQATSQ TLSIAYGTGS MTGILGYDTV QVGGIADTNQ IFGLSETEPG SFLYYSPFDG 190 200 210 220 230 240 ILGLAYPSIS SSGATPVFDN IWNQDLVSQD LFSVYLSSND QSGSVVMFGG IDSSYYTGSL 250 260 270 280 290 300 NWVPVSAEGY WQITVDSITM NGEAIACAEG CQAIVDTGTS LLSGPTSPIA NIQSYIGASE 310 320 330 340 350 360 NSNGEMVVSC SAISSLPDIV FTINGIQYPV PASAYILQDE GGCTSGFQGM NIPTAYGELW 370 380 ILGDVFIRQY FAVFDRANNQ VGLAPVA

References

[1]

"New World monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution." Kageyama T. J. Biochem. 127:761-770(2000) [PubMed: 10788784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 16-25, FUNCTION, ENZYME REGULATION. Tissue: Gastric mucosa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB038384 mRNA. Translation: BAA90871.1.
PIR	JC7245.
3D structure databases
SMR	Q9N2D4. Positions 16-387.
ModBase	Search...
Protein family/group databases
MEROPS	A01.001.
Genome annotation databases
Ensembl	ENSCJAT00000038063; ENSCJAP00000036045; ENSCJAG00000019394; Callithrix jacchus. [Genome view]
Phylogenomic databases
HOVERGEN	Q9N2D4.
Enzyme and pathway databases
BRENDA	3.4.23.1. 265156.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPA_CALJA

Accession

Primary (citable) accession number: Q9N2D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)