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Q9N2D2

- CHYM_CALJA

UniProt

Q9N2D2 - CHYM_CALJA

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Protein

Chymosin

Gene

CYM

Organism
Callithrix jacchus (White-tufted-ear marmoset)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes a variety of proteins.1 Publication

Catalytic activityi

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.

Enzyme regulationi

Inhibited by pepstatin.1 Publication

pH dependencei

Optimum pH is about 2.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921PROSITE-ProRule annotation
Active sitei274 – 2741PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiA01.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymosin (EC:3.4.23.4)
Alternative name(s):
Preprorennin
Gene namesi
Name:CYM
OrganismiCallithrix jacchus (White-tufted-ear marmoset)
Taxonomic identifieri9483 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniPlatyrrhiniCebidaeCallitrichinaeCallithrix
ProteomesiUP000008225: Chromosome 7

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16161 PublicationAdd
BLAST
Propeptidei17 – 5842Activation peptideBy similarityPRO_0000025990Add
BLAST
Chaini59 – 381323ChymosinPRO_0000025991Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi105 ↔ 110By similarity
Disulfide bondi265 ↔ 269By similarity
Disulfide bondi308 ↔ 341By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Developmental stagei

Expressed in adult, not neonate-specific as in other organisms.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9N2D2.
SMRiQ9N2D2. Positions 60-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati92 – 102111Add
BLAST
Repeati274 – 284112Add
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiQ9N2D2.
OMAiQYFGKIY.
OrthoDBiEOG7HQN88.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N2D2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRGFVVLLAV FALSQASGIV RIPLHKGKSL RRALKERGLL EDFLKNHQHA
60 70 80 90 100
VSRKHSNSRE VASEFLTNYL DCQYFGKIYI GTPPQEFTVV FDTGSSDLWV
110 120 130 140 150
PSVYCNSVAC QNHHRFDPSK SSTFQNMDKS LSIQYGTGSM QGLLGYDTVT
160 170 180 190 200
VSSIVDPHQT VGLSTQEPGD VFTYSEFDGI LGLAYPSLAS EYSVPVFDNM
210 220 230 240 250
MDRHLVAQDL FSVYMSRNEQ GSMLTLGAID PSYYTGSLHW IPVTVQEYWQ
260 270 280 290 300
FTVDSVTVDG VVVACDGGCQ AILDTGTSML VGPGSDIFNI QQAIGATEGQ
310 320 330 340 350
YGEFDIDCGT LSSMPTVVFE INGKKYPLPP SAYTNQDQGF CTSGFQGDDS
360 370 380
SQQWILGDVF IREYYSVFDR ASNLVGLAKA I
Length:381
Mass (Da):41,896
Last modified:October 1, 2000 - v1
Checksum:iC5820C74C97BB96B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038386 mRNA. Translation: BAA90873.1.
PIRiJC7247.
RefSeqiXP_002751243.1. XM_002751197.2.

Genome annotation databases

EnsembliENSCJAT00000006617; ENSCJAP00000006271; ENSCJAG00000003447.
GeneIDi100411198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038386 mRNA. Translation: BAA90873.1 .
PIRi JC7247.
RefSeqi XP_002751243.1. XM_002751197.2.

3D structure databases

ProteinModelPortali Q9N2D2.
SMRi Q9N2D2. Positions 60-381.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi A01.006.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCJAT00000006617 ; ENSCJAP00000006271 ; ENSCJAG00000003447 .
GeneIDi 100411198.

Phylogenomic databases

GeneTreei ENSGT00760000118929.
HOGENOMi HOG000197681.
HOVERGENi HBG000482.
InParanoidi Q9N2D2.
OMAi QYFGKIY.
OrthoDBi EOG7HQN88.
TreeFami TF314990.

Family and domain databases

Gene3Di 2.40.70.10. 2 hits.
InterProi IPR001461. Aspartic_peptidase.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR021109. Peptidase_aspartic_dom.
[Graphical view ]
PANTHERi PTHR13683. PTHR13683. 1 hit.
Pfami PF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view ]
PRINTSi PR00792. PEPSIN.
SUPFAMi SSF50630. SSF50630. 1 hit.
PROSITEi PS00141. ASP_PROTEASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "New World monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution."
    Kageyama T.
    J. Biochem. 127:761-770(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-26, FUNCTION, ENZYME REGULATION.
    Tissue: Gastric mucosa.

Entry informationi

Entry nameiCHYM_CALJA
AccessioniPrimary (citable) accession number: Q9N2D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3