Q9N2D2 (CHYM_CALJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chymosin
EC=3.4.23.4

Alternative name(s):
Preprorennin

Gene names

Name:

CYM

Organism

Callithrix jacchus (White-tufted-ear marmoset) [Reference proteome]

Taxonomic identifier

9483 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Platyrrhini › Cebidae › Callitrichinae › Callithrix

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes a variety of proteins. Ref.1
Catalytic activity	Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.
Enzyme regulation	Inhibited by pepstatin. Ref.1
Subunit structure	Monomer By similarity.
Developmental stage	Expressed in adult, not neonate-specific as in other organisms.
Sequence similarities	Belongs to the peptidase A1 family.
Biophysicochemical properties	pH dependence: Optimum pH is about 2.5.

Ontologies

Keywords
Biological process	Digestion
Domain	Repeat Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.1
	Propeptide	17 – 58	42	Activation peptide By similarity		PRO_0000025990
	Chain	59 – 381	323	Chymosin		PRO_0000025991
Regions
	Repeat	92 – 102	11	1
	Repeat	274 – 284	11	2
Sites
	Active site	92	1	By similarity
	Active site	274	1	By similarity
Amino acid modifications
	Disulfide bond	105 ↔ 110		By similarity
	Disulfide bond	265 ↔ 269		By similarity
	Disulfide bond	308 ↔ 341		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9N2D2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C5820C74C97BB96B	FASTA	381	41,896
	10 20 30 40 50 60 MRGFVVLLAV FALSQASGIV RIPLHKGKSL RRALKERGLL EDFLKNHQHA VSRKHSNSRE 70 80 90 100 110 120 VASEFLTNYL DCQYFGKIYI GTPPQEFTVV FDTGSSDLWV PSVYCNSVAC QNHHRFDPSK 130 140 150 160 170 180 SSTFQNMDKS LSIQYGTGSM QGLLGYDTVT VSSIVDPHQT VGLSTQEPGD VFTYSEFDGI 190 200 210 220 230 240 LGLAYPSLAS EYSVPVFDNM MDRHLVAQDL FSVYMSRNEQ GSMLTLGAID PSYYTGSLHW 250 260 270 280 290 300 IPVTVQEYWQ FTVDSVTVDG VVVACDGGCQ AILDTGTSML VGPGSDIFNI QQAIGATEGQ 310 320 330 340 350 360 YGEFDIDCGT LSSMPTVVFE INGKKYPLPP SAYTNQDQGF CTSGFQGDDS SQQWILGDVF 370 380 IREYYSVFDR ASNLVGLAKA I

References

[1]

"New World monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution." Kageyama T. J. Biochem. 127:761-770(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-26, FUNCTION, ENZYME REGULATION. Tissue: Gastric mucosa.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB038386 mRNA. Translation: BAA90873.1.
PIR	JC7247.
RefSeq	XP_002751243.1. XM_002751197.1.
3D structure databases
ProteinModelPortal	Q9N2D2.
SMR	Q9N2D2. Positions 60-381.
ModBase	Search...
Protein family/group databases
MEROPS	A01.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCJAT00000006617; ENSCJAP00000006271; ENSCJAG00000003447.
GeneID	100411198.
Phylogenomic databases
GeneTree	ENSGT00670000097830.
HOGENOM	HOG000197681.
HOVERGEN	HBG000482.
OMA	REYYSVF.
OrthoDB	EOG46HG9X.
Family and domain databases
Gene3D	2.40.70.10. 2 hits.
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR012848. Propep_A1. [Graphical view]
PANTHER	PTHR13683. PTHR13683. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SUPFAM	SSF50630. Pept_Aspartic. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHYM_CALJA

Accession

Primary (citable) accession number: Q9N2D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program