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Q9N2D2

- CHYM_CALJA

UniProt

Q9N2D2 - CHYM_CALJA

Protein

Chymosin

Gene

CYM

Organism
Callithrix jacchus (White-tufted-ear marmoset)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Hydrolyzes a variety of proteins.1 Publication

    Catalytic activityi

    Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.

    Enzyme regulationi

    Inhibited by pepstatin.1 Publication

    pH dependencei

    Optimum pH is about 2.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei92 – 921PROSITE-ProRule annotation
    Active sitei274 – 2741PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiA01.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chymosin (EC:3.4.23.4)
    Alternative name(s):
    Preprorennin
    Gene namesi
    Name:CYM
    OrganismiCallithrix jacchus (White-tufted-ear marmoset)
    Taxonomic identifieri9483 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniPlatyrrhiniCebidaeCallitrichinaeCallithrix
    ProteomesiUP000008225: Chromosome 7

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 16161 PublicationAdd
    BLAST
    Propeptidei17 – 5842Activation peptideBy similarityPRO_0000025990Add
    BLAST
    Chaini59 – 381323ChymosinPRO_0000025991Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi105 ↔ 110By similarity
    Disulfide bondi265 ↔ 269By similarity
    Disulfide bondi308 ↔ 341By similarity

    Keywords - PTMi

    Disulfide bond, Zymogen

    Expressioni

    Developmental stagei

    Expressed in adult, not neonate-specific as in other organisms.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9N2D2.
    SMRiQ9N2D2. Positions 60-381.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati92 – 102111Add
    BLAST
    Repeati274 – 284112Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase A1 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    GeneTreeiENSGT00670000097830.
    HOGENOMiHOG000197681.
    HOVERGENiHBG000482.
    OMAiQYFGKIY.
    OrthoDBiEOG7HQN88.
    TreeFamiTF314990.

    Family and domain databases

    Gene3Di2.40.70.10. 2 hits.
    InterProiIPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view]
    PANTHERiPTHR13683. PTHR13683. 1 hit.
    PfamiPF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view]
    PRINTSiPR00792. PEPSIN.
    SUPFAMiSSF50630. SSF50630. 1 hit.
    PROSITEiPS00141. ASP_PROTEASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9N2D2-1 [UniParc]FASTAAdd to Basket

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    MRGFVVLLAV FALSQASGIV RIPLHKGKSL RRALKERGLL EDFLKNHQHA    50
    VSRKHSNSRE VASEFLTNYL DCQYFGKIYI GTPPQEFTVV FDTGSSDLWV 100
    PSVYCNSVAC QNHHRFDPSK SSTFQNMDKS LSIQYGTGSM QGLLGYDTVT 150
    VSSIVDPHQT VGLSTQEPGD VFTYSEFDGI LGLAYPSLAS EYSVPVFDNM 200
    MDRHLVAQDL FSVYMSRNEQ GSMLTLGAID PSYYTGSLHW IPVTVQEYWQ 250
    FTVDSVTVDG VVVACDGGCQ AILDTGTSML VGPGSDIFNI QQAIGATEGQ 300
    YGEFDIDCGT LSSMPTVVFE INGKKYPLPP SAYTNQDQGF CTSGFQGDDS 350
    SQQWILGDVF IREYYSVFDR ASNLVGLAKA I 381
    Length:381
    Mass (Da):41,896
    Last modified:October 1, 2000 - v1
    Checksum:iC5820C74C97BB96B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038386 mRNA. Translation: BAA90873.1.
    PIRiJC7247.
    RefSeqiXP_002751243.1. XM_002751197.1.

    Genome annotation databases

    EnsembliENSCJAT00000006617; ENSCJAP00000006271; ENSCJAG00000003447.
    GeneIDi100411198.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038386 mRNA. Translation: BAA90873.1 .
    PIRi JC7247.
    RefSeqi XP_002751243.1. XM_002751197.1.

    3D structure databases

    ProteinModelPortali Q9N2D2.
    SMRi Q9N2D2. Positions 60-381.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi A01.006.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCJAT00000006617 ; ENSCJAP00000006271 ; ENSCJAG00000003447 .
    GeneIDi 100411198.

    Phylogenomic databases

    GeneTreei ENSGT00670000097830.
    HOGENOMi HOG000197681.
    HOVERGENi HBG000482.
    OMAi QYFGKIY.
    OrthoDBi EOG7HQN88.
    TreeFami TF314990.

    Family and domain databases

    Gene3Di 2.40.70.10. 2 hits.
    InterProi IPR001461. Aspartic_peptidase.
    IPR001969. Aspartic_peptidase_AS.
    IPR012848. Aspartic_peptidase_N.
    IPR021109. Peptidase_aspartic_dom.
    [Graphical view ]
    PANTHERi PTHR13683. PTHR13683. 1 hit.
    Pfami PF07966. A1_Propeptide. 1 hit.
    PF00026. Asp. 1 hit.
    [Graphical view ]
    PRINTSi PR00792. PEPSIN.
    SUPFAMi SSF50630. SSF50630. 1 hit.
    PROSITEi PS00141. ASP_PROTEASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "New World monkey pepsinogens A and C, and prochymosins. Purification, characterization of enzymatic properties, cDNA cloning, and molecular evolution."
      Kageyama T.
      J. Biochem. 127:761-770(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-26, FUNCTION, ENZYME REGULATION.
      Tissue: Gastric mucosa.

    Entry informationi

    Entry nameiCHYM_CALJA
    AccessioniPrimary (citable) accession number: Q9N2D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3