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Protein

Chymosin

Gene

CYM

Organism
Callithrix jacchus (White-tufted-ear marmoset)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes a variety of proteins.1 Publication

Catalytic activityi

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single 104-Ser-Phe-|-Met-Ala-107 bond in kappa-chain of casein.

Enzyme regulationi

Inhibited by pepstatin.1 Publication

pH dependencei

Optimum pH is about 2.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92PROSITE-ProRule annotation1
Active sitei274PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiA01.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymosin (EC:3.4.23.4)
Alternative name(s):
Preprorennin
Gene namesi
Name:CYM
OrganismiCallithrix jacchus (White-tufted-ear marmoset)
Taxonomic identifieri9483 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniPlatyrrhiniCebidaeCallitrichinaeCallithrix
Proteomesi
  • UP000008225 Componenti: Chromosome 7

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
PropeptideiPRO_000002599017 – 58Activation peptideBy similarityAdd BLAST42
ChainiPRO_000002599159 – 381ChymosinAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi105 ↔ 110By similarity
Disulfide bondi265 ↔ 269By similarity
Disulfide bondi308 ↔ 341By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Developmental stagei

Expressed in adult, not neonate-specific as in other organisms.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9483.ENSCJAP00000006271.

Structurei

3D structure databases

ProteinModelPortaliQ9N2D2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 378Peptidase A1PROSITE-ProRule annotationAdd BLAST305
Repeati92 – 1021Add BLAST11
Repeati274 – 2842Add BLAST11

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiQ9N2D2.
KOiK01378.
OMAiYFGKIYI.
OrthoDBiEOG091G0JP7.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033142. Chymosin.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF294. PTHR13683:SF294. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N2D2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGFVVLLAV FALSQASGIV RIPLHKGKSL RRALKERGLL EDFLKNHQHA
60 70 80 90 100
VSRKHSNSRE VASEFLTNYL DCQYFGKIYI GTPPQEFTVV FDTGSSDLWV
110 120 130 140 150
PSVYCNSVAC QNHHRFDPSK SSTFQNMDKS LSIQYGTGSM QGLLGYDTVT
160 170 180 190 200
VSSIVDPHQT VGLSTQEPGD VFTYSEFDGI LGLAYPSLAS EYSVPVFDNM
210 220 230 240 250
MDRHLVAQDL FSVYMSRNEQ GSMLTLGAID PSYYTGSLHW IPVTVQEYWQ
260 270 280 290 300
FTVDSVTVDG VVVACDGGCQ AILDTGTSML VGPGSDIFNI QQAIGATEGQ
310 320 330 340 350
YGEFDIDCGT LSSMPTVVFE INGKKYPLPP SAYTNQDQGF CTSGFQGDDS
360 370 380
SQQWILGDVF IREYYSVFDR ASNLVGLAKA I
Length:381
Mass (Da):41,896
Last modified:October 1, 2000 - v1
Checksum:iC5820C74C97BB96B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038386 mRNA. Translation: BAA90873.1.
PIRiJC7247.
RefSeqiXP_002751243.1. XM_002751197.2.

Genome annotation databases

EnsembliENSCJAT00000006617; ENSCJAP00000006271; ENSCJAG00000003447.
GeneIDi100411198.
KEGGicjc:100411198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038386 mRNA. Translation: BAA90873.1.
PIRiJC7247.
RefSeqiXP_002751243.1. XM_002751197.2.

3D structure databases

ProteinModelPortaliQ9N2D2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9483.ENSCJAP00000006271.

Protein family/group databases

MEROPSiA01.006.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCJAT00000006617; ENSCJAP00000006271; ENSCJAG00000003447.
GeneIDi100411198.
KEGGicjc:100411198.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000197681.
HOVERGENiHBG000482.
InParanoidiQ9N2D2.
KOiK01378.
OMAiYFGKIYI.
OrthoDBiEOG091G0JP7.
TreeFamiTF314990.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR012848. Aspartic_peptidase_N.
IPR033142. Chymosin.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF294. PTHR13683:SF294. 1 hit.
PfamiPF07966. A1_Propeptide. 1 hit.
PF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHYM_CALJA
AccessioniPrimary (citable) accession number: Q9N2D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: October 1, 2000
Last modified: October 5, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.