Reviewed, UniProtKB/Swiss-Prot Q9N2D1 (TRYT_PIG)

Names and origin

Protein names

Recommended name:
    Tryptase
    EC=3.4.21.59

Gene names

Name:

MCT7

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.
Subunit structure	Homotetramer By similarity.
Subcellular location	Secreted. Note: Released from the secretory granules upon mast cell activation.
Sequence similarities	Belongs to the peptidase S1 family. Tryptase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 30	10	Activation peptide By similarity		PRO_0000027486
	Chain	31 – 275	245	Tryptase		PRO_0000027487
Regions
	Domain	31 – 272	242	Peptidase S1
Sites
	Active site	74	1	Charge relay system By similarity
	Active site	121	1	Charge relay system By similarity
	Active site	224	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	132	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Disulfide bond	59 ↔ 75		By similarity
	Disulfide bond	155 ↔ 230		By similarity
	Disulfide bond	188 ↔ 211		By similarity
	Disulfide bond	220 ↔ 248		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9N2D1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: ACC582647FCCB973	FASTA	275	30,439
	10 20 30 40 50 60 MLNLLVLALP LLVSLVHTAP APGQALERAG IVGGKEAPGH KWPWQVSLRC LDQYWKHFCG 70 80 90 100 110 120 GSLIHPQWVL TAAHCFGPEK ADPLYIRVQL GEQHLYYQDR LLLVSRIIVH PNYYDEVNGA 130 140 150 160 170 180 DIALLELEDP VNLSSHVQPV TLPPASETFP KGTRCWVTGW GDVHSGWPLP PPYPLKQVRV 190 200 210 220 230 240 PIVENSECDM QYHLGLSTGD NIPIVRDDML CAGSEGHDSC QGDSGGPLVC RVNGTWLQAG 250 260 270 VVSWGEGCAL PNRPGIYTRV THYLDWIHQC IPRES

References

[1]

"Mast cell tryptase from pig lungs triggers infection by pneumotropic Sendai and influenza A viruses. Purification and characterization." Chen Y., Shiota M., Ohuchi M., Towatari T., Tashiro J., Murakami M., Yano M., Yang B., Kido H. Eur. J. Biochem. 267:3189-3197(2000) [PubMed: 10824103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB038652 mRNA. Translation: BAA93614.1.
RefSeq	NP_999356.1.
UniGene	Ssc.5614
3D structure databases
SMR	Q9N2D1. Positions 31-273.
ModBase	Search...
Protein family/group databases
MEROPS	S01.118.
Genome annotation databases
GeneID	397389.
KEGG	ssc:397389.
Organism-specific databases
CTD	397389.
Phylogenomic databases
HOVERGEN	Q9N2D1.
Enzyme and pathway databases
BRENDA	3.4.21.59. 249.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYT_PIG

Accession

Primary (citable) accession number: Q9N2D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	October 1, 2000
Last modified:	November 24, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)