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Protein

Pulmonary surfactant-associated protein D

Gene

SFTPD

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Gaseous exchange, Immunity, Innate immunity

Keywords - Ligandi

Calcium, Lectin

Enzyme and pathway databases

ReactomeiR-SSC-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-SSC-391160. Signal regulatory protein (SIRP) family interactions.
R-SSC-5683826. Surfactant metabolism.
R-SSC-5686938. Regulation of TLR by endogenous ligand.

Names & Taxonomyi

Protein namesi
Recommended name:
Pulmonary surfactant-associated protein D
Short name:
PSP-D
Short name:
SP-D
Alternative name(s):
Lung surfactant protein D
Gene namesi
Name:SFTPD
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted, Surface film

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_500005606021 – 378Pulmonary surfactant-associated protein DAdd BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35S-nitrosocysteineBy similarity1
Modified residuei40S-nitrosocysteineBy similarity1
Modified residuei784-hydroxyprolineBy similarity1
Modified residuei875-hydroxylysineBy similarity1
Glycosylationi90N-linked (GlcNAc...)Sequence analysis1
Modified residuei964-hydroxyprolineBy similarity1
Modified residuei995-hydroxylysineBy similarity1
Modified residuei1714-hydroxyprolineBy similarity1
Modified residuei1774-hydroxyprolineBy similarity1
Disulfide bondi281 ↔ 376PROSITE-ProRule annotation
Glycosylationi323N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi354 ↔ 368PROSITE-ProRule annotation

Post-translational modificationi

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.By similarity
S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation, S-nitrosylation

Proteomic databases

PaxDbiQ9N1X4.
PeptideAtlasiQ9N1X4.
PRIDEiQ9N1X4.

Expressioni

Gene expression databases

BgeeiENSSSCG00000010334.
GenevisibleiQ9N1X4. SS.

Interactioni

Subunit structurei

Oligomeric complex of 4 set of homotrimers.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000029281.

Structurei

Secondary structure

1378
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi225 – 253Combined sources29
Turni254 – 256Combined sources3
Beta strandi257 – 260Combined sources4
Beta strandi263 – 272Combined sources10
Helixi274 – 283Combined sources10
Beta strandi286 – 288Combined sources3
Helixi294 – 307Combined sources14
Beta strandi311 – 316Combined sources6
Beta strandi318 – 320Combined sources3
Helixi347 – 349Combined sources3
Beta strandi354 – 357Combined sources4
Beta strandi363 – 366Combined sources4
Beta strandi372 – 378Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DN8X-ray2.20A223-378[»]
ProteinModelPortaliQ9N1X4.
SMRiQ9N1X4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 222Collagen-likeAdd BLAST177
Domaini260 – 378C-type lectinPROSITE-ProRule annotationAdd BLAST119

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili223 – 254Sequence analysisAdd BLAST32

Sequence similaritiesi

Belongs to the SFTPD family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 collagen-like domain.Curated

Keywords - Domaini

Coiled coil, Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiQ9N1X4.
KOiK10068.
OMAiAENCVEI.
OrthoDBiEOG091G0MYV.
TreeFamiTF330481.

Family and domain databases

Gene3Di1.20.5.360. 1 hit.
3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR008160. Collagen.
IPR016187. CTDL_fold.
IPR015097. Surfac_D-trimer.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00059. Lectin_C. 1 hit.
PF09006. Surfac_D-trimer. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N1X4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLPLSVLI LLTQPPRSLG AEMKTYSQRA VANACALVMC SPMENGLPGR
60 70 80 90 100
DGRDGREGPR GEKGDPGLPG AVGRAGMPGL AGPVGPKGDN GSTGEPGAKG
110 120 130 140 150
DIGPCGPPGP PGIPGPAGKE GPSGQQGNIG PPGTPGPKGE TGPKGEVGAL
160 170 180 190 200
GMQGSTGARG PAGLKGERGA PGERGAPGSA GAAGPAGATG PQGPSGARGP
210 220 230 240 250
PGLKGDRGPP GERGAKGESG LPGITALRQQ VETLQGQVQR LQKAFSQYKK
260 270 280 290 300
VELFPNGRGV GEKIFKTGGF EKTFQDAQQV CTQAGGQMAS PRSETENEAL
310 320 330 340 350
SQLVTAQNKA AFLSMTDIKT EGNFTYPTGE PLVYANWAPG EPNNNGGSSG
360 370
AENCVEIFPN GKWNDKACGE LRLVICEF
Length:378
Mass (Da):37,986
Last modified:October 1, 2000 - v1
Checksum:i3504E8C1E56C341D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132496 mRNA. Translation: AAF22145.2.
RefSeqiNP_999275.1. NM_214110.1.
XP_013838830.1. XM_013983376.1.
UniGeneiSsc.319.

Genome annotation databases

EnsembliENSSSCT00000011312; ENSSSCP00000011020; ENSSSCG00000010334.
ENSSSCT00000034629; ENSSSCP00000029281; ENSSSCG00000010334.
GeneIDi397198.
KEGGissc:397198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132496 mRNA. Translation: AAF22145.2.
RefSeqiNP_999275.1. NM_214110.1.
XP_013838830.1. XM_013983376.1.
UniGeneiSsc.319.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DN8X-ray2.20A223-378[»]
ProteinModelPortaliQ9N1X4.
SMRiQ9N1X4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000029281.

Proteomic databases

PaxDbiQ9N1X4.
PeptideAtlasiQ9N1X4.
PRIDEiQ9N1X4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000011312; ENSSSCP00000011020; ENSSSCG00000010334.
ENSSSCT00000034629; ENSSSCP00000029281; ENSSSCG00000010334.
GeneIDi397198.
KEGGissc:397198.

Organism-specific databases

CTDi6441.

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104102.
HOGENOMiHOG000085660.
HOVERGENiHBG108270.
InParanoidiQ9N1X4.
KOiK10068.
OMAiAENCVEI.
OrthoDBiEOG091G0MYV.
TreeFamiTF330481.

Enzyme and pathway databases

ReactomeiR-SSC-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-SSC-391160. Signal regulatory protein (SIRP) family interactions.
R-SSC-5683826. Surfactant metabolism.
R-SSC-5686938. Regulation of TLR by endogenous ligand.

Gene expression databases

BgeeiENSSSCG00000010334.
GenevisibleiQ9N1X4. SS.

Family and domain databases

Gene3Di1.20.5.360. 1 hit.
3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR008160. Collagen.
IPR016187. CTDL_fold.
IPR015097. Surfac_D-trimer.
[Graphical view]
PfamiPF01391. Collagen. 1 hit.
PF00059. Lectin_C. 1 hit.
PF09006. Surfac_D-trimer. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSFTPD_PIG
AccessioniPrimary (citable) accession number: Q9N1X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.