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Q9N1F5

- GSTO1_PIG

UniProt

Q9N1F5 - GSTO1_PIG

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Protein

Glutathione S-transferase omega-1

Gene

GSTO1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid (By similarity).By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei32 – 321NucleophileBy similarity
Binding sitei59 – 591GlutathioneBy similarity
Binding sitei72 – 721Glutathione; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. glutathione dehydrogenase (ascorbate) activity Source: UniProtKB
  2. glutathione transferase activity Source: UniProtKB
  3. methylarsonate reductase activity Source: UniProtKB-EC
  4. oxidoreductase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to arsenic-containing substance Source: UniProtKB
  2. L-ascorbic acid metabolic process Source: UniProtKB
  3. xenobiotic catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase omega-1 (EC:2.5.1.18)
Short name:
GSTO-1
Alternative name(s):
Glutathione S-transferase omega 1-1
Short name:
GSTO 1-1
Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1)
Monomethylarsonic acid reductase (EC:1.20.4.2)
Short name:
MMA(V) reductase
S-(Phenacyl)glutathione reductase
Short name:
SPG-R
Gene namesi
Name:GSTO1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 14

Subcellular locationi

Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 241240Glutathione S-transferase omega-1PRO_0000185886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei57 – 571N6-acetyllysineBy similarity
Modified residuei143 – 1431N6-acetyllysineBy similarity
Modified residuei148 – 1481N6-acetyllysineBy similarity
Modified residuei152 – 1521N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9N1F5.

Expressioni

Tissue specificityi

Most abundant in the liver and skeletal muscle; also expressed in heart, diaphragm, colon, thymus, kidney, lung, ovaries, spleen, intestine and pancreas.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011310.

Structurei

3D structure databases

ProteinModelPortaliQ9N1F5.
SMRiQ9N1F5. Positions 5-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 10180GST N-terminalAdd
BLAST
Domaini106 – 225120GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni85 – 862Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00390000005479.
HOGENOMiHOG000006560.
HOVERGENiHBG051853.
InParanoidiQ9N1F5.
KOiK00799.
OMAiHKAYLDS.
OrthoDBiEOG71CFNG.
TreeFamiTF105325.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01625. GSTRNSFRASEO.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N1F5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGGSARSLG KGSAPPGPVP EGLIRVYSMR FCPFAQRTLL VLNAKGIRHQ
60 70 80 90 100
VININLKNKP EWFFQKNPSG LVPVLENSQG QLIYESAITC EYLDEAYPGK
110 120 130 140 150
KLLPDDPYEK ACQKMVFELS SKVPPLLIRF IRRENEADCS GLKEELRKEF
160 170 180 190 200
SKLEEVLTKK KTTYFGGSSL SMIDYLIWPW FERLEALELN ECIDHTPKLK
210 220 230 240
LWMAAMMKDP AVSALHIEPR DLRAFNDLYL QNSPEACDYG L
Length:241
Mass (Da):27,419
Last modified:January 31, 2002 - v2
Checksum:iAA50EE81C70433A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391C → Y AA sequence (PubMed:11485575)Curated
Sequence conflicti158 – 1581T → Q AA sequence (PubMed:11485575)Curated

Mass spectrometryi

Molecular mass is 27328±3 Da from positions 2 - 241. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF188838 mRNA. Translation: AAF71994.2.
RefSeqiNP_999215.1. NM_214050.1.
UniGeneiSsc.58505.

Genome annotation databases

EnsembliENSSSCT00000011607; ENSSSCP00000011305; ENSSSCG00000022351.
GeneIDi397117.
KEGGissc:397117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF188838 mRNA. Translation: AAF71994.2 .
RefSeqi NP_999215.1. NM_214050.1.
UniGenei Ssc.58505.

3D structure databases

ProteinModelPortali Q9N1F5.
SMRi Q9N1F5. Positions 5-241.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000011310.

Proteomic databases

PaxDbi Q9N1F5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000011607 ; ENSSSCP00000011305 ; ENSSSCG00000022351 .
GeneIDi 397117.
KEGGi ssc:397117.

Organism-specific databases

CTDi 9446.

Phylogenomic databases

eggNOGi COG0625.
GeneTreei ENSGT00390000005479.
HOGENOMi HOG000006560.
HOVERGENi HBG051853.
InParanoidi Q9N1F5.
KOi K00799.
OMAi HKAYLDS.
OrthoDBi EOG71CFNG.
TreeFami TF105325.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
PRINTSi PR01625. GSTRNSFRASEO.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification and characterization of a glutathione S-transferase Omega in pig: evidence for two distinct organ-specific transcripts."
    Rouimi P., Anglade P., Benzekri A., Costet P., Debrauwer L., Pineau T., Tulliez J.
    Biochem. J. 358:257-262(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 5-31; 58-83; 101-110; 115-147; 149-160; 162-190; 201-228 AND 235-241, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Tissue: Liver.

Entry informationi

Entry nameiGSTO1_PIG
AccessioniPrimary (citable) accession number: Q9N1F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 31, 2002
Last modified: October 29, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3