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Q9N1F5

- GSTO1_PIG

UniProt

Q9N1F5 - GSTO1_PIG

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Protein

Glutathione S-transferase omega-1

Gene
GSTO1
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid By similarity.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei32 – 321Nucleophile By similarity
Binding sitei59 – 591Glutathione By similarity
Binding sitei72 – 721Glutathione; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. glutathione dehydrogenase (ascorbate) activity Source: UniProtKB
  2. glutathione transferase activity Source: UniProtKB
  3. methylarsonate reductase activity Source: UniProtKB-EC
  4. oxidoreductase activity Source: UniProtKB

GO - Biological processi

  1. cellular response to arsenic-containing substance Source: UniProtKB
  2. L-ascorbic acid metabolic process Source: UniProtKB
  3. xenobiotic catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase omega-1 (EC:2.5.1.18)
Short name:
GSTO-1
Alternative name(s):
Glutathione S-transferase omega 1-1
Short name:
GSTO 1-1
Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.1)
Monomethylarsonic acid reductase (EC:1.20.4.2)
Short name:
MMA(V) reductase
S-(Phenacyl)glutathione reductase
Short name:
SPG-R
Gene namesi
Name:GSTO1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 14

Subcellular locationi

Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 241240Glutathione S-transferase omega-1PRO_0000185886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei57 – 571N6-acetyllysine By similarity
Modified residuei143 – 1431N6-acetyllysine By similarity
Modified residuei148 – 1481N6-acetyllysine By similarity
Modified residuei152 – 1521N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9N1F5.

Expressioni

Tissue specificityi

Most abundant in the liver and skeletal muscle; also expressed in heart, diaphragm, colon, thymus, kidney, lung, ovaries, spleen, intestine and pancreas.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000011310.

Structurei

3D structure databases

ProteinModelPortaliQ9N1F5.
SMRiQ9N1F5. Positions 5-241.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 10180GST N-terminalAdd
BLAST
Domaini106 – 225120GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni85 – 862Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00390000005479.
HOGENOMiHOG000006560.
HOVERGENiHBG051853.
KOiK00799.
OMAiHKAYLDS.
OrthoDBiEOG71CFNG.
TreeFamiTF105325.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
PRINTSiPR01625. GSTRNSFRASEO.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N1F5-1 [UniParc]FASTAAdd to Basket

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MSGGSARSLG KGSAPPGPVP EGLIRVYSMR FCPFAQRTLL VLNAKGIRHQ    50
VININLKNKP EWFFQKNPSG LVPVLENSQG QLIYESAITC EYLDEAYPGK 100
KLLPDDPYEK ACQKMVFELS SKVPPLLIRF IRRENEADCS GLKEELRKEF 150
SKLEEVLTKK KTTYFGGSSL SMIDYLIWPW FERLEALELN ECIDHTPKLK 200
LWMAAMMKDP AVSALHIEPR DLRAFNDLYL QNSPEACDYG L 241
Length:241
Mass (Da):27,419
Last modified:January 31, 2002 - v2
Checksum:iAA50EE81C70433A6
GO

Mass spectrometryi

Molecular mass is 27328±3 Da from positions 2 - 241. Determined by ESI. 1 Publication

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391C → Y AA sequence 1 Publication
Sequence conflicti158 – 1581T → Q AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF188838 mRNA. Translation: AAF71994.2.
RefSeqiNP_999215.1. NM_214050.1.
UniGeneiSsc.58505.

Genome annotation databases

EnsembliENSSSCT00000011607; ENSSSCP00000011305; ENSSSCG00000022351.
GeneIDi397117.
KEGGissc:397117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF188838 mRNA. Translation: AAF71994.2 .
RefSeqi NP_999215.1. NM_214050.1.
UniGenei Ssc.58505.

3D structure databases

ProteinModelPortali Q9N1F5.
SMRi Q9N1F5. Positions 5-241.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000011310.

Proteomic databases

PaxDbi Q9N1F5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000011607 ; ENSSSCP00000011305 ; ENSSSCG00000022351 .
GeneIDi 397117.
KEGGi ssc:397117.

Organism-specific databases

CTDi 9446.

Phylogenomic databases

eggNOGi COG0625.
GeneTreei ENSGT00390000005479.
HOGENOMi HOG000006560.
HOVERGENi HBG051853.
KOi K00799.
OMAi HKAYLDS.
OrthoDBi EOG71CFNG.
TreeFami TF105325.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005442. GST_omega.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
PRINTSi PR01625. GSTRNSFRASEO.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification and characterization of a glutathione S-transferase Omega in pig: evidence for two distinct organ-specific transcripts."
    Rouimi P., Anglade P., Benzekri A., Costet P., Debrauwer L., Pineau T., Tulliez J.
    Biochem. J. 358:257-262(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 5-31; 58-83; 101-110; 115-147; 149-160; 162-190; 201-228 AND 235-241, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Tissue: Liver.

Entry informationi

Entry nameiGSTO1_PIG
AccessioniPrimary (citable) accession number: Q9N1F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 31, 2002
Last modified: May 14, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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