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Protein

Protein MRVI1

Gene

MRVI1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as NO/PRKG1-dependent regulator of IP3-induced calcium release; its phosphorylation by PRKG1 inhibits bradykinin and IP3-induced calcium release from intracellular stores. Recruits PRKG1 to the endoplasmic reticulum and may mediate the assembly of PRKG1 and ITPR1 in a macrocomplex. Involved in PRKG1 signaling cascade leading to inhibition of platelet activation and aggregation. Mediates also NO-dependent inhibition of calcium signaling in gastrointestinal smooth muscle contributing to NO-dependent relaxation.2 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MRVI1
Alternative name(s):
Inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate
JAW1-related protein MRVI1
Gene namesi
Name:MRVI1
Synonyms:IRAG
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei853 – 87321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Sarcoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi118 – 1181S → A: No change in the inhibition of calcium release and no change in phosphorylation efficiency; when associated with A-393 and A-683. 1 Publication
Mutagenesisi172 – 1721R → A: Drastic decrease of PRKG1 binding; when associated with A-176. 1 Publication
Mutagenesisi176 – 1761R → A: Drastic decrease of PRKG1 binding; when associated with A-172. Disruption of PRKG1 interaction; when associated with A-177. 1 Publication
Mutagenesisi177 – 1771R → A: Drastic decrease of PRKG1 binding; when associated with A-176. 1 Publication
Mutagenesisi179 – 1791R → A: Reduced interaction with PRKG1; when associated with A-180. 1 Publication
Mutagenesisi180 – 1801K → A: Reduced interaction with PRKG1; when associated with A-179. Reduced interaction with PRKG1; when associated with A-182. 1 Publication
Mutagenesisi182 – 1821R → A: Reduced interaction with PRKG1; when associated with A-180. 1 Publication
Mutagenesisi393 – 3931S → A: No change in the inhibition of calcium release and no change in phosphorylation efficiency; when associated with A-118 and A-683. No more calcium release inhibition; when associated with A-118; A-683 and A-696. 1 Publication
Mutagenesisi683 – 6831S → A: No change in the inhibition of calcium release and no change in phosphorylation efficiency; when associated with A-118 and A-393. No more calcium release inhibition; when associated with A-118; A-393 and A-696. 1 Publication
Mutagenesisi696 – 6961S → A: Drastic phosphorylation decrease by PRKG1 and no more inhibition of calcium release. No calcium release inhibition; when associated with A-118; A-393 and A-683. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911Protein MRVI1PRO_0000305291Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei118 – 1181Phosphoserine1 Publication
Modified residuei393 – 3931Phosphoserine1 Publication
Modified residuei683 – 6831Phosphoserine1 Publication
Modified residuei696 – 6961Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by PRKG1/cGKI-beta. Phosphorylation at Ser-696 is necessary for PRKG1-induced calcium release in the cytosol.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9N1F0.
PRIDEiQ9N1F0.

PTM databases

iPTMnetiQ9N1F0.

Miscellaneous databases

PMAP-CutDBQ9N1F0.

Expressioni

Tissue specificityi

Highly expressed in trachea, aorta and uterus.1 Publication

Interactioni

Subunit structurei

Interacts with PRKG1/cGKI-beta and ITPR1/IP3R type I. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1.4 Publications

Protein-protein interaction databases

IntActiQ9N1F0. 1 interaction.
STRINGi9913.ENSBTAP00000043465.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 18433Interaction with PRKG1Add
BLAST
Regioni534 – 58047Interaction with ITPR1By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili547 – 64599Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi768 – 83568Glu-richAdd
BLAST

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG5G. Eukaryota.
ENOG410XVI5. LUCA.
HOGENOMiHOG000113653.
HOVERGENiHBG078204.
InParanoidiQ9N1F0.

Family and domain databases

InterProiIPR008677. MRVI1.
[Graphical view]
PANTHERiPTHR15352. PTHR15352. 1 hit.
PfamiPF05781. MRVI1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9N1F0-1) [UniParc]FASTAAdd to basket

Also known as: IRAGa

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVKAPQSEER LAGGGKGNNS VLACGAQASW SIFGADAAEV PGTRSHSRQE
60 70 80 90 100
AAMPHIPEDE EPPGEPQAAQ SPAGQDPATT GISCSPPTII LTGDASSPEG
110 120 130 140 150
ETDKNPVNRA HSPHRRLSHR HLKVSTASLT SVDPAGHVID LVNDQLPDIS
160 170 180 190 200
ISEEDKKKNL ALLEEAKLVS ERFLTRRGRK SRSSPGESSP AVSPNLSPGA
210 220 230 240 250
SPASSQSNSL TVPTPPGLDV CSGPPSPLPG APPQKGDEAE VPSPHLGESN
260 270 280 290 300
VLKGLADRKQ NDQRTLSQGR LTARSPTVEK SKEITIEQKE NFDPLQRPEA
310 320 330 340 350
IPKGPASGPG SGGKMALNSP QPGPVESELG KPLAKTAKEG NPLPRGPTQG
360 370 380 390 400
SGGVAPQASQ GKSTVGEPAG SKVGSKAELW PPTSRPPLLR GVSWDSGPEE
410 420 430 440 450
PGPRLQKVLA KLPLAEEEKR FTGKAGSKLA KAPGLKDFQI QVQPVRMQKL
460 470 480 490 500
TKLREEHILL RNQNLVGLKL PELSEAAEQE KGHPSELSSA IEEEESKGGL
510 520 530 540 550
DVMPNISDVL LRKLRVHKSL PGSAPPLTEK EVENVFVQLS LAFRNDSYTL
560 570 580 590 600
ESRINQAERE RNLTEENTEK ELENFKASIT SSASLWHHCE HRETYQKLLE
610 620 630 640 650
DIAVLHRLAA RLSSRAEMVG AVRQEKRMSK ATEVMMQYVE NLKRTYEKDH
660 670 680 690 700
AELMEFKKLA NQNSSRSCGP SEDGVPRTAR SMSLSLGKNM PRRRVSVAVV
710 720 730 740 750
PKFNILNLPG QSPSSSPIPS LPALSESSNG KGNPPVSSAL PALLENGKTN
760 770 780 790 800
GDPDCEASAS VPTPSCLEGI SQEAKARMEE EAYNKGYQEG LKKTKELQGL
810 820 830 840 850
REEEEEQKSE SPEEPEEVAE TEEEEKEQRS SKLEELVHFL QVMYPKLCQH
860 870 880 890 900
WQVIWMMAAA MLVLTVVLGL YGSHNSCVEQ ADGSLGKSTC SAAQRDSWWS
910
SGLQHEQPTE Q
Length:911
Mass (Da):98,375
Last modified:October 1, 2000 - v1
Checksum:iD743EFA5A28FF62D
GO
Isoform 2 (identifier: Q9N1F0-2) [UniParc]FASTAAdd to basket

Also known as: IRAGb

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.

Show »
Length:859
Mass (Da):93,137
Checksum:iCFD39CF2EAFB99B2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5252Missing in isoform 2. 1 PublicationVSP_028340Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195526 mRNA. Translation: AAF61202.1.
AF195527 mRNA. Translation: AAF61203.1.
UniGeneiBt.5302.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195526 mRNA. Translation: AAF61202.1.
AF195527 mRNA. Translation: AAF61203.1.
UniGeneiBt.5302.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9N1F0. 1 interaction.
STRINGi9913.ENSBTAP00000043465.

PTM databases

iPTMnetiQ9N1F0.

Proteomic databases

PaxDbiQ9N1F0.
PRIDEiQ9N1F0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IG5G. Eukaryota.
ENOG410XVI5. LUCA.
HOGENOMiHOG000113653.
HOVERGENiHBG078204.
InParanoidiQ9N1F0.

Miscellaneous databases

NextBioi20805810.
PMAP-CutDBQ9N1F0.

Family and domain databases

InterProiIPR008677. MRVI1.
[Graphical view]
PANTHERiPTHR15352. PTHR15352. 1 hit.
PfamiPF05781. MRVI1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Regulation of intracellular calcium by a signalling complex of IRAG, IP3 receptor and cGMP kinase Ibeta."
    Schlossmann J., Ammendola A., Ashman K., Zong X., Huber A., Neubauer G., Wang G.-X., Allescher H.-D., Korth M., Wilm M., Hofmann F., Ruth P.
    Nature 404:197-201(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, PHOSPHORYLATION AT SER-118; SER-393; SER-683 AND SER-696, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INTERACTION WITH PRKG1 AND ITPR1.
  2. "Molecular determinants of the interaction between the inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate (IRAG) and cGMP kinase Ibeta."
    Ammendola A., Geiselhoeringer A., Hofmann F., Schlossmann J.
    J. Biol. Chem. 276:24153-24159(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRKG1 AND ITPR1, REGION, MUTAGENESIS OF SER-118; SER-393; SER-683 AND SER-696.
  3. Cited for: SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY.
  4. "Identification of the interface between cGMP-dependent protein kinase Ibeta and its interaction partners TFII-I and IRAG reveals a common interaction motif."
    Casteel D.E., Boss G.R., Pilz R.B.
    J. Biol. Chem. 280:38211-38218(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRKG1, MUTAGENESIS OF ARG-172; ARG-176; ARG-177; ARG-179; LYS-180 AND ARG-182.

Entry informationi

Entry nameiMRVI1_BOVIN
AccessioniPrimary (citable) accession number: Q9N1F0
Secondary accession number(s): Q9N1E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 1, 2000
Last modified: March 16, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.