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Protein

Protein 4.1

Gene

EPB41

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 4.1
Short name:
P4.1
Alternative name(s):
4.1R
Band 4.1
Gene namesi
Name:EPB41
Synonyms:E41P
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 617617Protein 4.1PRO_0000219388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131PhosphotyrosineBy similarity
Modified residuei331 – 3311PhosphoserineBy similarity
Modified residuei333 – 3331PhosphoserineBy similarity
Modified residuei413 – 4131Phosphotyrosine; by EGFRBy similarity
Modified residuei417 – 4171PhosphoserineBy similarity
Modified residuei427 – 4271PhosphoserineBy similarity
Modified residuei462 – 4621PhosphoserineBy similarity
Modified residuei465 – 4651Phosphoserine; by CDK1By similarity

Post-translational modificationi

Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.By similarity
Phosphorylation on Tyr-413 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9N179.

Interactioni

Subunit structurei

Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin and DLG1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9N179.
SMRiQ9N179. Positions 1-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 282282FERMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni401 – 46666Spectrin--actin-bindingAdd
BLAST
Regioni467 – 617151C-terminal (CTD)Add
BLAST

Sequence similaritiesi

Contains 1 FERM domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000228841.
HOVERGENiHBG007777.
InParanoidiQ9N179.
KOiK06107.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR008379. Band_4.1_C.
IPR019749. Band_41_domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR007477. SAB_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF05902. 4_1_CTD. 1 hit.
PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF04382. SAB. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9N179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED YFGLAIWDNA
60 70 80 90 100
TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED ITRYYLCLQL
110 120 130 140 150
RQDIVSGRLP CSFATLALLG SYTIQSELGD YDPELHGADY VSDFKLAPNQ
160 170 180 190 200
TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD LHKAKDLEGV
210 220 230 240 250
DIILGVCSSG LLVYKEKLRI NRFPWPKVLK ISYKRSSFFI KIRPGEQEQY
260 270 280 290 300
ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK FLALGSKFRY
310 320 330 340 350
SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAV EPADRTPRPT
360 370 380 390 400
SAPAIAPSPA AEGGVPGAPV KKAQKETVQV EVKQEEAPPE DAEPEPSEAW
410 420 430 440 450
KKKRERLDGE NIYIRHSNLM LEDLDKSQEE IKKHHASISE LKKNFMESVP
460 470 480 490 500
EPRPSEWDKR LSTHSPFRTL NINGQIPTGE GPPLVKTQTV TISDTANAVK
510 520 530 540 550
SEIPTKDVPI VHTETKTITY EAAQTDDSNG DLDPGVLLTA QTITSETTSS
560 570 580 590 600
TTTTQITKTV KGGISETRIE KRIVITGDAD IDHDQVLVQA IKEAKEQHPD
610
MSVTKVVVHQ ETEISEE
Length:617
Mass (Da):69,258
Last modified:October 1, 2000 - v1
Checksum:i5B49D5008AD900FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222767 mRNA. Translation: AAF61703.1.
RefSeqiNP_776736.1. NM_174311.1.
UniGeneiBt.87209.

Genome annotation databases

GeneIDi281753.
KEGGibta:281753.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222767 mRNA. Translation: AAF61703.1.
RefSeqiNP_776736.1. NM_174311.1.
UniGeneiBt.87209.

3D structure databases

ProteinModelPortaliQ9N179.
SMRiQ9N179. Positions 1-279.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9N179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281753.
KEGGibta:281753.

Organism-specific databases

CTDi2035.

Phylogenomic databases

HOGENOMiHOG000228841.
HOVERGENiHBG007777.
InParanoidiQ9N179.
KOiK06107.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR008379. Band_4.1_C.
IPR019749. Band_41_domain.
IPR000798. Ez/rad/moesin-like.
IPR014847. FERM-adjacent.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR011993. PH_dom-like.
IPR007477. SAB_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF05902. 4_1_CTD. 1 hit.
PF08736. FA. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF04382. SAB. 1 hit.
[Graphical view]
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiSM00295. B41. 1 hit.
SM01195. FA. 1 hit.
SM01196. FERM_C. 1 hit.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The interaction of bovine red blood cell protein 4.1 with red blood cell membranes."
    Saito D., Inaba M., Koshino I., Matsumoto M., Ono K.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Japanese black.
    Tissue: Bone marrow.

Entry informationi

Entry namei41_BOVIN
AccessioniPrimary (citable) accession number: Q9N179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.