Reviewed,
UniProtKB/Swiss-Prot Q9N119 (3BHS_PIG)
Last modified
February 9, 2010.
Version 56.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Short name=3-beta-HSD Including the following 2 domains: 1- Recommended name: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase EC=1.1.1.145 Alternative name(s): 3-beta-hydroxy-5-ene steroid dehydrogenase Progesterone reductase 2- Recommended name: Steroid Delta-isomerase EC=5.3.3.1 Alternative name(s): Delta-5-3-ketosteroid isomerase | ||||
| Gene names |
| ||||
| Organism | Sus scrofa (Pig) | ||||
| Taxonomic identifier | 9823 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus |
Protein attributes
| Sequence length | 373 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. |
| Catalytic activity | A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH. A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid. |
| Pathway | |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. |
| Sequence similarities | Belongs to the 3-beta-HSD family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Steroidogenesis |
| Cellular component | Endoplasmic reticulum Membrane Mitochondrion |
| Domain | Transmembrane |
| Ligand | NAD |
| Molecular function | Isomerase Oxidoreductase |
| Technical term | Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membraneInferred from electronic annotation. Source: UniProtKB-SubCell mitochondrionInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 3-beta-hydroxy-delta5-steroid dehydrogenase activity Inferred from electronic annotation. Source: EC bindingInferred from electronic annotation. Source: InterPro steroid delta-isomerase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity UniProtKB P14893 | ||||||
| Chain | 2 – 373 | 372 | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase | PRO_0000087786 | |||||
Regions | |||||||||
| Transmembrane | 292 – 309 | 18 | Potential | ||||||
Sites | |||||||||
| Active site | 155 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 159 | 1 | NAD By similarity | ||||||
Sequences
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References
| [1] | "cDNA cloning and physical mapping of porcine 3 beta-hydroxysteroid dehydrogenase/Delta 5-delta 4 isomerase." von Teichman A., Joerg H., Werner P., Brenig B., Stranzinger G. Anim. Genet. 32:298-302(2001) [PubMed: 11683717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adipose tissue. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF232699 mRNA. Translation: AAF37295.2. |
| RefSeq | NP_001004049.1. |
| UniGene | Ssc.14393 |
3D structure databases | |
| SMR | Q9N119. Positions 5-364. |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | ENSSSCT00000007360; ENSSSCP00000007165; ENSSSCG00000006719; Sus scrofa. [Genome view] |
| GeneID | 445539. |
| KEGG | ssc:445539. |
Organism-specific databases | |
| CTD | 445539. |
Phylogenomic databases | |
| HOVERGEN | Q9N119. |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.145. 249. 5.3.3.1. 249. |
Family and domain databases | |
| InterPro | IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | 3BHS_PIG | ||||||||
| Accession | Primary (citable) accession number: Q9N119 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


