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Q9N119 (3BHS_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase

Short name=3-beta-HSD

Including the following 2 domains:

  1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
    EC=1.1.1.145
    Alternative name(s):
    3-beta-hydroxy-5-ene steroid dehydrogenase
    Progesterone reductase
  2. Steroid Delta-isomerase
    EC=5.3.3.1
    Alternative name(s):
    Delta-5-3-ketosteroid isomerase
Gene names
Name:HSD3B
Synonyms:3b-HSD
OrganismSus scrofa (Pig) [Complete proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activity

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.

Sequence similarities

Belongs to the 3-beta-HSD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity UniProtKB P14893
Chain2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
PRO_0000087786

Regions

Transmembrane292 – 30918Helical; Potential

Sites

Active site1551Proton acceptor By similarity
Binding site1591NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9N119 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 8BD4FEEF0117F6FF

FASTA37341,882
        10         20         30         40         50         60 
MAGWSCLVTG GGGFLGQRIV HLLLEEKDLQ EIRVLDKVFK PEVREEFSKL QSKIKLTMLE 

        70         80         90        100        110        120 
GDILDEQCLK GACQGASVVI HTASIIDVVN AVGRETVMKV NVKGTQLLLE ACVQASVPVF 

       130        140        150        160        170        180 
IHTSSIEVAG PNSYREVIQN ACEEDRLETA WSAPYPLSKK LAEKAVLEAN GWALQNGGTL 

       190        200        210        220        230        240 
HTCALRPMYI YGEGSPFIFA HMNKALENNG VLTHNSKFSR VNPVYVGNVA WAHILALRAL 

       250        260        270        280        290        300 
RDPRKALSVQ GQFYYVADDT PPQSYDDLNY TLGKEWGFCL DSRRSLPPSL RYWLAFLLEI 

       310        320        330        340        350        360 
VSFLLSPIYN YQPPFNRHFV TLCNSVFTVS YKKAQRDLGY EPLFTWEEAK QKTKAWVGSL 

       370 
VKQHKEALKT KTH 

« Hide

References

[1]"cDNA cloning and physical mapping of porcine 3 beta-hydroxysteroid dehydrogenase/Delta 5-delta 4 isomerase."
von Teichman A., Joerg H., Werner P., Brenig B., Stranzinger G.
Anim. Genet. 32:298-302(2001) [PubMed: 11683717] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adipose tissue.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF232699 mRNA. Translation: AAF37295.2.
RefSeqNP_001004049.1. NM_001004049.1.
UniGeneSsc.14393.

3D structure databases

ProteinModelPortalQ9N119.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9N119.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000007360; ENSSSCP00000007165; ENSSSCG00000006719.
GeneID445539.
KEGGssc:445539.

Organism-specific databases

CTD3283.

Phylogenomic databases

GeneTreeENSGT00550000074557.
HOVERGENHBG000014.
OMAMAGWSCL.
OrthoDBEOG4K3KWG.

Enzyme and pathway databases

BRENDA1.1.1.145. 6170.

Family and domain databases

InterProIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00070.
PfamPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry name3BHS_PIG
AccessionPrimary (citable) accession number: Q9N119
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 71 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families