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Protein

Sodium/potassium-transporting ATPase subunit alpha-1

Gene

ATP1A1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity). May contribute to blood-heart barrier properties of endocardial endothelium and may control cardiac performance via endothelial Na+/H+ exchange.By similarity1 Publication

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei376 – 37614-aspartylphosphate intermediateBy similarity
Binding sitei487 – 4871ATPBy similarity
Metal bindingi717 – 7171MagnesiumBy similarity
Metal bindingi721 – 7211MagnesiumBy similarity

GO - Molecular functioni

  1. ATPase binding Source: BHF-UCL
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. nitric-oxide synthase binding Source: BHF-UCL
  5. sodium:potassium-exchanging ATPase activity Source: UniProtKB-EC

GO - Biological processi

  1. regulation of sodium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-1 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-1 subunit
Alternative name(s):
Sodium pump subunit alpha-1
Gene namesi
Name:ATP1A1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811 Componenti: Unplaced

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8782CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Topological domaini109 – 13123ExtracellularSequence AnalysisAdd
BLAST
Transmembranei132 – 15221HelicalSequence AnalysisAdd
BLAST
Topological domaini153 – 288136CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei289 – 30820HelicalSequence AnalysisAdd
BLAST
Topological domaini309 – 32012ExtracellularSequence AnalysisAdd
BLAST
Transmembranei321 – 33818HelicalSequence AnalysisAdd
BLAST
Topological domaini339 – 772434CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei773 – 79220HelicalSequence AnalysisAdd
BLAST
Topological domaini793 – 80210ExtracellularSequence Analysis
Transmembranei803 – 82321HelicalSequence AnalysisAdd
BLAST
Topological domaini824 – 84320CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei844 – 86623HelicalSequence AnalysisAdd
BLAST
Topological domaini867 – 91852ExtracellularSequence AnalysisAdd
BLAST
Transmembranei919 – 93820HelicalSequence AnalysisAdd
BLAST
Topological domaini939 – 95113CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei952 – 97019HelicalSequence AnalysisAdd
BLAST
Topological domaini971 – 98515ExtracellularSequence AnalysisAdd
BLAST
Transmembranei986 – 100621HelicalSequence AnalysisAdd
BLAST
Topological domaini1007 – 102317CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55By similarityPRO_0000305979
Chaini6 – 10231018Sodium/potassium-transporting ATPase subunit alpha-1By similarityPRO_0000305980Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91N6-acetyllysineBy similarity
Modified residuei10 – 101PhosphotyrosineBy similarity
Modified residuei16 – 161Phosphoserine; by PKCBy similarity
Modified residuei21 – 211N6-acetyllysineBy similarity
Modified residuei260 – 2601PhosphotyrosineBy similarity
Modified residuei542 – 5421PhosphotyrosineBy similarity
Modified residuei661 – 6611N6-succinyllysineBy similarity
Modified residuei943 – 9431Phosphoserine; by PKABy similarity

Post-translational modificationi

Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ9N0Z6.

Expressioni

Tissue specificityi

Expressed in endocardial endothelial cells.1 Publication

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with SIK1 (By similarity). Interacts with SLC35G1 and STIM1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ATP1B1Q9TT372EBI-9685690,EBI-9685670

Protein-protein interaction databases

IntActiQ9N0Z6. 2 interactions.
STRINGi9986.ENSOCUP00000024821.

Structurei

3D structure databases

ProteinModelPortaliQ9N0Z6.
SMRiQ9N0Z6. Positions 26-1023.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 843Phosphoinositide-3 kinase bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ9N0Z6.
KOiK01539.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N0Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKGVGRDKY EPAAVSEHGD KKGKKAKKER DMDELKKEVS MDDHKLSLDE
60 70 80 90 100
LHRKYGTDLS RGLTTARAAE ILARDGPNAL TPPPTTPEWV KFCRQLFGGF
110 120 130 140 150
SMLLWIGAIL CFLAYGILAA TEEDFDNDNL YLGVVLAAVV IITGCFSYYQ
160 170 180 190 200
EAKSSKIMES FKNMVPQQAL VIRNGEKMSI NAEDVVVGDL VEVKGGDRIP
210 220 230 240 250
ADLRIISANG CKVDNSSLTG ESEPQTRSPD FTNENPLETR NIAFFSTNCV
260 270 280 290 300
EGTARGIVIY TGDRTVMGRI ATLASGLEGG QTPIAAEIEH FIHIITGVAV
310 320 330 340 350
FLGVSFFILS LILEYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR
360 370 380 390 400
MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA
410 420 430 440 450
DTTENQSGVS FDKTSATWLA LSRIAGLCNR AVFQANQENL PILKRAVAGD
460 470 480 490 500
ASESALLKCI ELCCGSVKEM RERYTKIVEI PFNSTNKYQL SIHKNLNANE
510 520 530 540 550
PRHLLVMKGA PERILDRCSS ILLHGKEQPL DEELKDAFQN AYLELGGLGE
560 570 580 590 600
RVLGFCHLLL PDEQFPEGFQ FDTDEVNFPV DNLCFIGLIS MIDPPRAAVP
610 620 630 640 650
DAVGKCRSAG IKVIMVTGDH PITAKAIAKG VGIISEGNET VEDIAARLNI
660 670 680 690 700
PVSQVNPRDA KACVVHGSDL KDMTSEQLDD ILKYHTEIVF ARTSPQQKLI
710 720 730 740 750
IVEGCQRQGA IVAVTGDGVN DSPALKKADI GVAMGIAGSD VSKQAADMIL
760 770 780 790 800
LDDNFASIVT GVEEGRLIFD NLKKSIAYTL TSNIPEITPF LIFIIANIPL
810 820 830 840 850
PLGTVTILCI DLGTDMVPAI SLAYEQAESD IMKRQPRNPK TDKLVNERLI
860 870 880 890 900
SMAYGQIGMI QALGGFFTYF VILAENGFLP FHLLGIRVDW DDRWINDVED
910 920 930 940 950
SYGQQWTYEQ RKIVEFTCHT AFFVSIVVVQ WADLVICKTR RNSVFQQGMK
960 970 980 990 1000
NKILIFGLFE ETALAAFLSY CPGMGVALRM YPLKPTWWFC AFPYSLLIFV
1010 1020
YDEIRKLIIR RRPGGWVEKE TYY
Length:1,023
Mass (Da):112,994
Last modified:February 29, 2004 - v2
Checksum:iF6BA59C8979F574D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF235024 mRNA. Translation: AAF60310.2.
RefSeqiNP_001156546.1. NM_001163074.1.
UniGeneiOcu.2457.

Genome annotation databases

GeneIDi100302415.
KEGGiocu:100302415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF235024 mRNA. Translation: AAF60310.2.
RefSeqiNP_001156546.1. NM_001163074.1.
UniGeneiOcu.2457.

3D structure databases

ProteinModelPortaliQ9N0Z6.
SMRiQ9N0Z6. Positions 26-1023.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9N0Z6. 2 interactions.
STRINGi9986.ENSOCUP00000024821.

Proteomic databases

PRIDEiQ9N0Z6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100302415.
KEGGiocu:100302415.

Organism-specific databases

CTDi476.

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ9N0Z6.
KOiK01539.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Distribution and role of Na(+)/K(+) ATPase in endocardial endothelium."
    Fransen P., Hendrickx J., Brutsaert D.L., Sys S.U.
    Cardiovasc. Res. 52:487-499(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiAT1A1_RABIT
AccessioniPrimary (citable) accession number: Q9N0Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2007
Last sequence update: February 29, 2004
Last modified: March 3, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.