Reviewed, UniProtKB/Swiss-Prot Q9N0W2 (FUT8_BOVIN)

Names and origin

Protein names

Recommended name:
    Alpha-(1,6)-fucosyltransferase
    EC=2.4.1.68
Alternative name(s):
    Glycoprotein 6-alpha-L-fucosyltransferase
    GDP-fucose--glycoprotein fucosyltransferase
    GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase
      Short name=alpha1-6FucT
    Fucosyltransferase 8

Gene names

Name:

FUT8

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans By similarity.
Catalytic activity	GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl-(1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Tissue specificity	Highest expression found in brain. Also found in heart, lung, spleen and kidney.
Sequence similarities	Belongs to the glycosyltransferase 23 family. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	SH3 domain SH3-binding Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	protein amino acid glycosylation in Golgi Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SH3 domain binding Inferred from electronic annotation. Source: UniProtKB-KW glycoprotein 6-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Alpha-(1,6)-fucosyltransferase		PRO_0000080525
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Signal-anchor for type II membrane protein Potential
	Topological domain	31 – 575	545	Lumenal Potential
	Domain	502 – 563	62	SH3
	Region	365 – 366	2	Important for donor substrate binding By similarity
	Motif	299 – 305	7	SH3-binding Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9N0W2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FE04C66B8A5BF540	FASTA	575	66,493
	10 20 30 40 50 60 MRPWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHPDHSS RELSKILAKL ERLKQQNEDL 70 80 90 100 110 120 RRMAESLRIP EGPIDQGPAS GRIRALEEQL VKAKEQIENY KKQTRNGLGK DHEILRRRIE 130 140 150 160 170 180 NGAKELWFFL QSELKKLKNL EGNELQRHAD EFLSDLGHHE RSIMTDLYYL SQTDGAGDWR 190 200 210 220 230 240 EKEAKDLTEL VQRRITYLQN PKDCSKAKKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT 250 260 270 280 290 300 LILESHNWRY ATGGWETVFR PVSETCTDRS GVYTGHWSGE IKDKNVQVVE LPIVDSLHPR 310 320 330 340 350 360 PPYLPLAVPE DLADRLVRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT KKLGFKHPVI 370 380 390 400 410 420 GVHVRRTDKV GTEAAFHPIE EYMVHVEEHF QLLARRMQVD KKRVYLATDD PSLLKEAKTK 430 440 450 460 470 480 YPHYEFISDN SISWSAGLHN RYTENSLRGV ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ 490 500 510 520 530 540 TLHPDASANF HSLDDIYYFG GQNAHNQIAI YPHEPRTADE IPMEPGDIIG VAGNHWDGYS 550 560 570 KGVNRKLGRT GLYPSYKVRE KIETVKVPHV PEAEK

References

[1]

"Ancestral exonic organization of FUT8, the gene encoding the alpha6-fucosyltransferase, reveals successive peptide domains which suggest a particular three-dimensional core structure for the alpha6-fucosyltransferase family." Javaud C., Dupuy F., Maftah A., Michalski J.-C., Oriol R., Petit J.-M., Julien R. Mol. Biol. Evol. 17:1661-1672(2000) [PubMed: 11070054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF247186 mRNA. Translation: AAF65460.1.
IPI	IPI00688497.
UniGene	Bt.270
3D structure databases
SMR	Q9N0W2. Positions 108-572.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9N0W2.
Protein family/group databases
CAZy	GT23. Glycosyltransferase Family 23.
Genome annotation databases
Ensembl	ENSBTAT00000026406; ENSBTAP00000026406; ENSBTAG00000019819; Bos taurus. [Genome view]
Phylogenomic databases
HOVERGEN	Q9N0W2.
InParanoid	Q9N0W2.
Enzyme and pathway databases
BRENDA	2.4.1.68. 251.
Family and domain databases
InterPro	IPR015827. Alpha1_6FUT_euk. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF000472. Alpha1_6FUT_euk. 1 hit.
SMART	SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT8_BOVIN

Accession

Primary (citable) accession number: Q9N0W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2000
Last modified:	December 15, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)