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Protein

Eukaryotic translation initiation factor 4E

Gene

EIF4E

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-BTA-1169408 ISG15 antiviral mechanism
R-BTA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-BTA-166208 mTORC1-mediated signalling
R-BTA-429947 Deadenylation of mRNA
R-BTA-72649 Translation initiation complex formation
R-BTA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S
R-BTA-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E
Short name:
eIF-4E
Short name:
eIF4E
Alternative name(s):
mRNA cap-binding protein
Gene namesi
Name:EIF4E
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 6

Organism-specific databases

VGNCiVGNC:28408 EIF4E

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001936332 – 217Eukaryotic translation initiation factor 4EAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei22PhosphothreonineBy similarity1
Modified residuei209Phosphoserine; by PKC and MKNK2By similarity1

Post-translational modificationi

Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9N0T5
PRIDEiQ9N0T5

PTM databases

iPTMnetiQ9N0T5

Expressioni

Gene expression databases

BgeeiENSBTAG00000009522

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Hypophosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (By similarity). Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1. Interacts with CLOCK (By similarity). Binds to MKNK2 in nucleus. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with LARP1. Interacts with METTL3 (By similarity). Interacts with RBM24; this interaction prevents EIF4E from binding to p53/TP53 mRNA and inhibits the assembly of translation initiation complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000012530

Structurei

3D structure databases

ProteinModelPortaliQ9N0T5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 40EIF4EBP1/2/3 bindingBy similarity4
Regioni56 – 577-methylguanosine-containing mRNA cap bindingBy similarity2
Regioni73 – 77EIF4EBP1/2/3 bindingBy similarity5
Regioni102 – 1037-methylguanosine-containing mRNA cap bindingBy similarity2
Regioni132 – 139EIF4EBP1/2/3 bindingBy similarity8
Regioni157 – 1627-methylguanosine-containing mRNA cap bindingBy similarity6
Regioni205 – 2077-methylguanosine-containing mRNA cap bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1670 Eukaryota
COG5053 LUCA
GeneTreeiENSGT00520000055549
HOGENOMiHOG000186751
HOVERGENiHBG006130
InParanoidiQ9N0T5
KOiK03259
OMAiMKMTIGY
OrthoDBiEOG091G0IVA
TreeFamiTF101526

Family and domain databases

Gene3Di3.30.760.10, 1 hit
InterProiView protein in InterPro
IPR023398 TIF_eIF4e-like
IPR001040 TIF_eIF_4E
IPR019770 TIF_eIF_4E_CS
PANTHERiPTHR11960 PTHR11960, 1 hit
PfamiView protein in Pfam
PF01652 IF4E, 1 hit
SUPFAMiSSF55418 SSF55418, 1 hit
PROSITEiView protein in PROSITE
PS00813 IF4E, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9N0T5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVEPETTP TPNPPPTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN
60 70 80 90 100
DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP
110 120 130 140 150
MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETVLCLIGE SFDDYSDDVC
160 170 180 190 200
GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH
210
ADTATKSGST TKNRFVV
Length:217
Mass (Da):25,079
Last modified:October 31, 2006 - v2
Checksum:i44BC0418D54BA583
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124S → G in AAF66991 (PubMed:11913777).Curated1
Sequence conflicti134V → L in AAF66991 (PubMed:11913777).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF257235 mRNA Translation: AAF66991.1
BC111272 mRNA Translation: AAI11273.1
RefSeqiNP_776735.2, NM_174310.3
UniGeneiBt.1148

Genome annotation databases

EnsembliENSBTAT00000012530; ENSBTAP00000012530; ENSBTAG00000009522
GeneIDi281751
KEGGibta:281751

Similar proteinsi

Entry informationi

Entry nameiIF4E_BOVIN
AccessioniPrimary (citable) accession number: Q9N0T5
Secondary accession number(s): Q2NL02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: October 31, 2006
Last modified: May 23, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

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