Reviewed, UniProtKB/Swiss-Prot Q9N0E1 (AACS_MACFA)

Names and origin

Protein names

Recommended name:
    Acetoacetyl-CoA synthetase
    EC=6.2.1.16

Gene names

Name:	AACS
ORF Names:	QccE-10783, QccE-14415, QflA-13650

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development By similarity.
Catalytic activity	ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetoacetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Acetoacetyl-CoA synthetase		PRO_0000315785
Amino acid modifications
	Modified residue	524	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	7	1	P → A in BAB41151. Ref.1
	Sequence conflict	110	1	E → D in BAE01877. Ref.2
	Sequence conflict	271	1	A → V in BAE01877. Ref.2
	Sequence conflict	337	1	M → V in BAE01877. Ref.2
	Sequence conflict	400	1	S → G in BAB41151. Ref.1
	Sequence conflict	400	1	S → G in BAE01877. Ref.2
	Sequence conflict	505	1	S → N in BAB41151. Ref.1
	Sequence conflict	505	1	S → N in BAE01877. Ref.2
	Sequence conflict	578	1	F → Y in BAE01877. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9N0E1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: DF31672A8E204528	FASTA	672	75,046
	10 20 30 40 50 60 MSKEERPGRE EILECQVMWE PDSKKNTQMD RFRAAVGAAC GLALENYDDL YRWSVESYSD 70 80 90 100 110 120 FWAEFWKFSG IVFSRAYDEV VDTSKGIADV PEWFKGSRLN YAENLLRHKE NDRVALYVAR 130 140 150 160 170 180 EGKEEIVKVT FEELRQEVAL FAAAMRKMGV KKGDRVVGYL PNSEHAVEAM LAAASIGAIW 190 200 210 220 230 240 SSTSPDFGVN GVLDRFSQIQ PKLIFSVEAV VYNGKEHSHM EKLQQVVKGL PDLKKVVVIP 250 260 270 280 290 300 YVSSREKIDL SKIPNSVFLD DFLATGTSEQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM 310 320 330 340 350 360 VHSAGGTLIQ HLKEHLLHGN MTSSDILLCY TTAGWMMWNW MVSILATGAA MVLYDGSPLV 370 380 390 400 410 420 PTPNVLWDLV DRIGITVLVT GAKWLSVLEE KAMKPVETHS LQMLHTILST GSPLKAQSYE 430 440 450 460 470 480 YVYRCIKSSI LLGSISGGTD IISCFMGHNF SLPVYKGEIQ ARNLGMAVEA WNEEGKAVWG 490 500 510 520 530 540 ESGELVCTKP IPCQPTHFWN DENGSKYRKA YFSKFPGIWA HGDYCRINPK TGGIIMLGRS 550 560 570 580 590 600 DGTLNPNGVR FGSSEIYNIV ESFEEVEDSL CVPQYNKFRE ERVILFLKMA SGHAFQPDLV 610 620 630 640 650 660 KRIRDAIRVG LSARHVPSLI LETKGIPYTL NGKKVEVAVK QIIAGKAVEQ GGAFSNPETL 670 DLYRDIPELQ GF

References

[1]	"Isolation of full-length cDNA clones from macaque brain cDNA libraries." Osada N., Hida M., Kusuda J., Tanuma R., Iseki K., Hirai M., Terao K., Suzuki Y., Sugano S., Hashimoto K. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex and Frontal cortex.
[2]	"DNA sequences of macaque genes expressed in brain or testis and its evolutionary implications." International consortium for macaque cDNA sequencing and analysis Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB045989 mRNA. Translation: BAB01571.1. AB060214 mRNA. Translation: BAB41151.1. AB169796 mRNA. Translation: BAE01877.1.
3D structure databases
SMR	Q9N0E1. Positions 34-666.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q9N0E1.
Enzyme and pathway databases
BRENDA	6.2.1.16. 3438.
Family and domain databases
InterPro	IPR005914. Acac_CoA_synth. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01217. ac_ac_CoA_syn. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AACS_MACFA

Accession

Primary (citable) accession number: Q9N0E1
Secondary accession number(s): Q4R4U8, Q9BE28

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)