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Protein

Caspase-1

Gene

CASP1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of MB21D1/cGAS, rendering it inactive.By similarity

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei237By similarity1
Active sitei285By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processApoptosis

Enzyme and pathway databases

BRENDAi3.4.22.36. 1153.

Protein family/group databases

MEROPSiC14.024.

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-1 (EC:3.4.22.36)
Short name:
CASP-1
Alternative name(s):
Interleukin-1 beta convertase
Short name:
IL-1BC
Interleukin-1 beta-converting enzyme
Short name:
ICE
Short name:
IL-1 beta-converting enzyme
p45
Cleaved into the following 2 chains:
Gene namesi
Name:CASP1
Synonyms:IL1BC
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000045091 – 119Sequence analysisAdd BLAST119
ChainiPRO_0000004510120 – 297Caspase-1 subunit p20Add BLAST178
PropeptideiPRO_0000004511298 – 316Sequence analysisAdd BLAST19
ChainiPRO_0000004512317 – 404Caspase-1 subunit p10Add BLAST88

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei302PhosphoserineBy similarity1

Post-translational modificationi

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Keywords - PTMi

Phosphoprotein, Zymogen

Proteomic databases

PaxDbiQ9MZV7.

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p10 and p20 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000021900.

Structurei

3D structure databases

ProteinModelPortaliQ9MZV7.
SMRiQ9MZV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 91CARDPROSITE-ProRule annotationAdd BLAST91

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiKOG3573. Eukaryota.
ENOG410ZQIE. LUCA.
HOGENOMiHOG000234399.
HOVERGENiHBG076981.
InParanoidiQ9MZV7.
KOiK04394.

Family and domain databases

CDDicd00032. CASc. 1 hit.
InterProiView protein in InterPro
IPR001315. CARD.
IPR029030. Caspase-like_dom.
IPR033139. Caspase_cys_AS.
IPR016129. Caspase_his_AS.
IPR011029. DEATH-like_dom.
IPR002138. Pept_C14_p10.
IPR001309. Pept_C14_p20.
IPR015917. Pept_C14A.
PfamiView protein in Pfam
PF00619. CARD. 1 hit.
PRINTSiPR00376. IL1BCENZYME.
SMARTiView protein in SMART
SM00114. CARD. 1 hit.
SM00115. CASc. 1 hit.
SUPFAMiSSF47986. SSF47986. 1 hit.
SSF52129. SSF52129. 1 hit.
PROSITEiView protein in PROSITE
PS50209. CARD. 1 hit.
PS01122. CASPASE_CYS. 1 hit.
PS01121. CASPASE_HIS. 1 hit.
PS50207. CASPASE_P10. 1 hit.
PS50208. CASPASE_P20. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9MZV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKVLKDKR RLFVRSVDMG TINGLLDELF EKRVLNHEEM ERVRCAHSTV
60 70 80 90 100
MDQARVLIDS VLRKGPNACQ IFISNICNED IHLAQTLGLS SGSPSGNDHT
110 120 130 140 150
KLDSQVEVPS LPAFVENMPG PTIPDSEEST DTLKLCPPET FVKMYKEKAE
160 170 180 190 200
EIYPIKERKD RTRLALIICN IEFDHLSTRD GAELDIAGME SLLEGLGYSV
210 220 230 240 250
VVKRKLTAKG MESVLREFAA RPEHKSSDST FLVLMSHGIL NGICGTAHSV
260 270 280 290 300
ENPDVLAYDT IFQIFNNRHC LNLKDKPKVI IIQACRGENP GELWVSDSPK
310 320 330 340 350
ASTDSWTHQP LMLQSDAIHK VHVEKDFIAF CSSTPHNVSW RHITKGSLFI
360 370 380 390 400
AQLITCFQKY SWCCHLEGVF RKVQQSFEKP DVKAQMPTIE RVSMTRYFYL

FPGH
Length:404
Mass (Da):45,662
Last modified:October 1, 2000 - v1
Checksum:iBC5A505CD7DBDFDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135967 mRNA. Translation: AAF64388.1.
RefSeqiNP_001003125.1. NM_001003125.1.
UniGeneiCfa.3589.

Genome annotation databases

GeneIDi403724.
KEGGicfa:403724.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCASP1_CANLF
AccessioniPrimary (citable) accession number: Q9MZV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 1, 2000
Last modified: July 5, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families