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Protein

Caspase-1

Gene

CASP1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive.By similarity

Catalytic activityi

Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei237By similarity1
Active sitei285By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processApoptosis

Enzyme and pathway databases

BRENDAi3.4.22.36 1153

Protein family/group databases

MEROPSiC14.024

Names & Taxonomyi

Protein namesi
Recommended name:
Caspase-1 (EC:3.4.22.36)
Short name:
CASP-1
Alternative name(s):
Interleukin-1 beta convertase
Short name:
IL-1BC
Interleukin-1 beta-converting enzyme
Short name:
ICE
Short name:
IL-1 beta-converting enzyme
p45
Cleaved into the following 2 chains:
Gene namesi
Name:CASP1
Synonyms:IL1BC
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000045091 – 119Sequence analysisAdd BLAST119
ChainiPRO_0000004510120 – 297Caspase-1 subunit p20Add BLAST178
PropeptideiPRO_0000004511298 – 316Sequence analysisAdd BLAST19
ChainiPRO_0000004512317 – 404Caspase-1 subunit p10Add BLAST88

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei302PhosphoserineBy similarity1

Post-translational modificationi

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Keywords - PTMi

Phosphoprotein, Zymogen

Proteomic databases

PaxDbiQ9MZV7
PRIDEiQ9MZV7

Interactioni

Subunit structurei

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p10 and p20 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000021900

Structurei

3D structure databases

ProteinModelPortaliQ9MZV7
SMRiQ9MZV7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 91CARDPROSITE-ProRule annotationAdd BLAST91

Sequence similaritiesi

Belongs to the peptidase C14A family.Curated

Phylogenomic databases

eggNOGiKOG3573 Eukaryota
ENOG410ZQIE LUCA
HOGENOMiHOG000234399
HOVERGENiHBG076981
InParanoidiQ9MZV7
KOiK04394

Family and domain databases

CDDicd00032 CASc, 1 hit
InterProiView protein in InterPro
IPR001315 CARD
IPR029030 Caspase-like_dom_sf
IPR033139 Caspase_cys_AS
IPR016129 Caspase_his_AS
IPR011029 DEATH-like_dom_sf
IPR002138 Pept_C14_p10
IPR001309 Pept_C14_p20
IPR015917 Pept_C14A
PfamiView protein in Pfam
PF00619 CARD, 1 hit
PRINTSiPR00376 IL1BCENZYME
SMARTiView protein in SMART
SM00114 CARD, 1 hit
SM00115 CASc, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
SSF52129 SSF52129, 1 hit
PROSITEiView protein in PROSITE
PS50209 CARD, 1 hit
PS01122 CASPASE_CYS, 1 hit
PS01121 CASPASE_HIS, 1 hit
PS50207 CASPASE_P10, 1 hit
PS50208 CASPASE_P20, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9MZV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKVLKDKR RLFVRSVDMG TINGLLDELF EKRVLNHEEM ERVRCAHSTV
60 70 80 90 100
MDQARVLIDS VLRKGPNACQ IFISNICNED IHLAQTLGLS SGSPSGNDHT
110 120 130 140 150
KLDSQVEVPS LPAFVENMPG PTIPDSEEST DTLKLCPPET FVKMYKEKAE
160 170 180 190 200
EIYPIKERKD RTRLALIICN IEFDHLSTRD GAELDIAGME SLLEGLGYSV
210 220 230 240 250
VVKRKLTAKG MESVLREFAA RPEHKSSDST FLVLMSHGIL NGICGTAHSV
260 270 280 290 300
ENPDVLAYDT IFQIFNNRHC LNLKDKPKVI IIQACRGENP GELWVSDSPK
310 320 330 340 350
ASTDSWTHQP LMLQSDAIHK VHVEKDFIAF CSSTPHNVSW RHITKGSLFI
360 370 380 390 400
AQLITCFQKY SWCCHLEGVF RKVQQSFEKP DVKAQMPTIE RVSMTRYFYL

FPGH
Length:404
Mass (Da):45,662
Last modified:October 1, 2000 - v1
Checksum:iBC5A505CD7DBDFDD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135967 mRNA Translation: AAF64388.1
RefSeqiNP_001003125.1, NM_001003125.1
UniGeneiCfa.3589

Genome annotation databases

GeneIDi403724
KEGGicfa:403724

Similar proteinsi

Entry informationi

Entry nameiCASP1_CANLF
AccessioniPrimary (citable) accession number: Q9MZV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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