Q9MZV6 (CASP1_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caspase-1
Short name=CASP-1
EC=3.4.22.36

Alternative name(s):
Interleukin-1 beta convertase
Short name=IL-1BC
Interleukin-1 beta-converting enzyme
Short name=ICE
Short name=IL-1 beta-converting enzyme
p45

Cleaved into the following 2 chains:

1. Caspase-1 subunit p20
2. Caspase-1 subunit p10

Gene names

Name:	CASP1
Synonyms:	IL1BC

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis By similarity.
Catalytic activity	Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|-.
Subunit structure	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Interacts with CARD17/INCA and CARD18 By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Sequence similarities	Belongs to the peptidase C14A family. Contains 1 CARD domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW regulation of apoptotic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 119	119	Potential		PRO_0000004513
	Chain	120 – 296	177	Caspase-1 subunit p20		PRO_0000004514
	Propeptide	297 – 322	26	Potential		PRO_0000004515
	Chain	323 – 410	88	Caspase-1 subunit p10		PRO_0000004516
Regions
	Domain	1 – 91	91	CARD
Sites
	Active site	235	1	By similarity
	Active site	284	1	By similarity
Natural variations
	Natural variant	136	1	H → R.

Sequences

	Sequence		Length	Mass (Da)
	Q9MZV6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 2B8BB99C007A40C5	FASTA	410	46,052
	10 20 30 40 50 60 MADKVLKGKR KQFINSVGMG TVNGLLDELF EKNVLNQEEM ERVKCENATV MDKARALIDS 70 80 90 100 110 120 VLRKGPRACQ IFICHICEED THLAETLGLS SSPQSGNSQN TTDSEVAFPP LPASVNNMPG 130 140 150 160 170 180 PAEPEESVDA LKLCPHENFV KLCKQRAEEI YPIKERKDRT RLALIICNTT FDHLSLRKGA 190 200 210 220 230 240 DLDVAGMRRL LTDLGYSVHI KEELTAKDME SELRAFAARP EHKSSDSTFL VFMSHGILSG 250 260 270 280 290 300 ICGTKYSAEG DPDVLAYDTI FQIFNNRNCL SLKDKPKVII VQACRGENLG ELLISDSPAA 310 320 330 340 350 360 PMDSTSQMGS SLSQVGDNLE DDAIYKVHVE KDFIAFCSST PHHVSWRDVN KGSLFITQLI 370 380 390 400 410 TCFQKYSWCF HLEEVFRKVQ QSFEKPNVRA QMPTIERLSM TRYFYLFPGH

References

[1]	"Cloning and sequencing of feline and canine ice-related cDNAs encoding hybrid caspase-1/caspase-13-like propeptides." Taylor S., Hanlon L., McGillivray C., Gault E.A., Argyle D.J., Onions D.E., Nicolson L. DNA Seq. 10:387-394(2000) [PubMed: 10826695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF135968 mRNA. Translation: AAF64389.1.
RefSeq	NP_001009365.1. NM_001009365.1.
3D structure databases
ProteinModelPortal	Q9MZV6.
SMR	Q9MZV6. Positions 2-90, 149-296, 323-410.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	493961.
Organism-specific databases
CTD	834.
Phylogenomic databases
HOVERGEN	HBG076981.
Enzyme and pathway databases
BRENDA	3.4.22.36. 2240.
Family and domain databases
InterPro	IPR001315. CARD. IPR017350. Caspase_IL-1_beta. IPR011029. DEATH-like. IPR011600. Pept_C14_cat. IPR001309. Pept_C14_ICE_p20. IPR016129. Pept_C14_ICE_p20_AS. IPR002138. Pept_C14_p10. IPR002398. Pept_C14_p45. IPR015917. Pept_C14_p45_core. [Graphical view]
Gene3D	G3DSA:1.10.533.10. DEATH_like. 1 hit.
PANTHER	PTHR10454. Pept_C14_p45. 1 hit.
Pfam	PF00619. CARD. 1 hit. PF00656. Peptidase_C14. 1 hit. [Graphical view]
PIRSF	PIRSF038001. Caspase_ICE. 1 hit.
PRINTS	PR00376. IL1BCENZYME.
SMART	SM00114. CARD. 1 hit. SM00115. CASc. 1 hit. [Graphical view]
SUPFAM	SSF47986. DEATH_like. 1 hit.
PROSITE	PS50209. CARD. 1 hit. PS01122. CASPASE_CYS. 1 hit. PS01121. CASPASE_HIS. 1 hit. PS50207. CASPASE_P10. 1 hit. PS50208. CASPASE_P20. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASP1_FELCA

Accession

Primary (citable) accession number: Q9MZV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program