Q9MTQ6 (RCCR_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Red chlorophyll catabolite reductase
Short name=RCC reductase
EC=1.3.1.80

Alternative name(s):
HvRCCR

Gene names

Name:

rccR

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	205 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the key reaction of chlorophyll catabolism, porphyrin macrocycle cleavage of pheophorbide a (pheide a) to a primary fluorescent catabolite (pFCC). Works in a two-step reaction with pheophorbide a oxygenase (PaO) by reducing the C20/C1 double bond of the intermediate, RCC.
Catalytic activity	Primary fluorescent chlorophyll catabolite + NADP+ = red chlorophyll catabolite + NADPH.
Pathway	Porphyrin degradation; chlorophyll degradation.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast stroma.
Tissue specificity	In etiolated and green primary leaves. Low amount in roots.
Developmental stage	Decreases during leaf senescence, but still present after 8 days of dark-induced senescence.
Post-translational modification	The N-terminus is blocked.

Ontologies

Keywords
Biological process	Chlorophyll catabolism
Cellular component	Chloroplast Plastid
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 205	›205	Red chlorophyll catabolite reductase		PRO_0000097198
Sites
	Binding site	39	1	Substrate Probable
	Binding site	175	1	Substrate Probable
	Site	103	1	Important for substrate stereospecificity By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q9MTQ6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 835DC48C813D5AD7	FASTA	205	22,868
	10 20 30 40 50 60 IDFMLQSSLH CKVPNGAIDI TSLFINLNAS TDAPHFIMEF IQGSPTSMVV LLDLLPRKDL 70 80 90 100 110 120 ALHPEYIEKY YEDTEVDKQR KIIEQLPQAR PYLSPSLFVR SAFSPTAVFF TIDCGKGGEG 130 140 150 160 170 180 TLEEIVHGHL ASVVKGILQI WLDTCASDAS EMEEGEREIM VKRDRTVRSK SIEVDLTANL 190 200 PRMFGPDVSG RIIAEIRKAF GVQEG

References

[1]

"Molecular cloning, functional expression and characterization of RCC reductase, involved in chlorophyll catabolism." Wuethrich K.L., Bovet L., Hunziker P.E., Donnison I.S., Hoertensteiner S. Plant J. 21:189-198(2000) [PubMed: 10743659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Express. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ243066 mRNA. Translation: CAB77705.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q9MTQ6.
Gene expression databases
Genevestigator	Q9MTQ6.
Family and domain databases
InterPro	IPR009439. RCC_reductase. [Graphical view]
Pfam	PF06405. RCC_reductase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RCCR_HORVU

Accession

Primary (citable) accession number: Q9MTQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	October 1, 2000
Last modified:	June 28, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program