Q9MT28 (THRC_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Threonine synthase, chloroplastic Short name=TS EC=4.2.3.1
Organism	Solanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine By similarity.
Catalytic activity	O-phospho-L-homoserine + H2O = L-threonine + phosphate.
Cofactor	Pyridoxal phosphate By similarity.
Enzyme regulation	Allosterically activated by S-adenosyl-methionine (SAM) By similarity.
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the threonine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate S-adenosyl-L-methionine
Molecular function	Lyase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro threonine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Chloroplast By similarity
	Chain	41 – 519	479	Threonine synthase, chloroplastic		PRO_0000033618
Regions
	Region	328 – 332	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	465	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	196	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9MT28 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 114C0979CD231464	FASTA	519	57,412
	10 20 30 40 50 60 MAASCMLRSS FISPGLPQLH HQSTSKPNNG IHFFTPIKAT ATNDAISQQK HRRPADENIR 70 80 90 100 110 120 EEARRHCSSH NFSARYVPFN AGPNSDEWYS LDEIVYRSRS GGLLDVQHDM DALKKFDGQY 130 140 150 160 170 180 WRSLFDSRVG KTTWPYGSGV WSKKEWVLPE IDSDDIVSAF EGNSNLFWAE RFGKQFLGMT 190 200 210 220 230 240 DLWVKHCGIS HTGSFKDLGM TVLVSQVNRL RKMHKPVVGV GCASTGDTSA ALSAYCASAG 250 260 270 280 290 300 IPSIVFLPAN KISMAQLVQP IANGAFVLSI DTDFDGCMQL IREVTAELPI YLANSLNSLR 310 320 330 340 350 360 LEGQKTAAIE ILQQFDWEVP EWVIVPGGNL GNIYAFYKGF QMCKELGLVD RIPRLVCAQA 370 380 390 400 410 420 ANANPLYLHY KSGWKDFKPV KANTTFASAI QIGDPVSIDR AVFALQQCNG IVEEATEEEL 430 440 450 460 470 480 MDAMAQADST GMFICPHTGV ALTALFKLRN SGVIAPTDRT VVVSTAHGLK FTQSKIDYHS 490 500 510 KEIKDMECRF ANPPVEVKAD FGSVMDVLKS YLLSQNSKL

References

[1]

"Expression of threonine synthase from Solanum tuberosum L. is not metabolically regulated by photosynthesis-related signals or by nitrogenous compounds." Casazza A.P., Basner A., Hoefgen R., Hesse H. Plant Sci. 157:43-50(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF082894 mRNA. Translation: AAF74984.1.
3D structure databases
ProteinModelPortal	Q9MT28.
SMR	Q9MT28. Positions 48-517.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00050; UER00065.
Family and domain databases
InterPro	IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS. IPR004450. Thr_synthase_like. IPR001926. Trp_syn_b_sub_like_PLP_eny_SF. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR00260. thrC. 1 hit.
PROSITE	PS00165. DEHYDRATASE_SER_THR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THRC_SOLTU

Accession

Primary (citable) accession number: Q9MT28

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program