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Q9MCN8

- RUSA_BPHK7

UniProt

Q9MCN8 - RUSA_BPHK7

Protein

Crossover junction endodeoxyribonuclease rusA

Gene

rusA

Organism
Enterobacteria phage HK97 (Bacteriophage HK97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity.

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701MagnesiumBy similarity
    Metal bindingi72 – 721MagnesiumBy similarity
    Metal bindingi91 – 911MagnesiumBy similarity

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-KW
    2. DNA repair Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease rusA (EC:3.1.22.4)
    Alternative name(s):
    Gp67
    Holliday junction nuclease rusA
    Holliday junction resolvase
    Gene namesi
    Name:rusA
    Synonyms:67
    OrganismiEnterobacteria phage HK97 (Bacteriophage HK97)
    Taxonomic identifieri37554 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000002576: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 120120Crossover junction endodeoxyribonuclease rusAPRO_0000192007Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9MCN8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RusA family.Curated

    Family and domain databases

    Gene3Di3.30.1330.70. 1 hit.
    InterProiIPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view]
    PfamiPF05866. RusA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001007. RusA. 1 hit.
    SUPFAMiSSF103084. SSF103084. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9MCN8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNEYQFVLPY PPSLNTYWRR RGSQYYISDK GQKYRKDVQQ IIRQLKLDIF    50
    TKSRLRIKVI ADVPDSRRRD LDNILKGLLD SLIHAGFAED DEQFDDIRVI 100
    RGVKVPGGRL GIKITELESV 120
    Length:120
    Mass (Da):14,067
    Last modified:October 1, 2000 - v1
    Checksum:i7D28A53BA6ED7398
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069529 Genomic DNA. Translation: AAF31142.1.
    RefSeqiNP_037749.1. NC_002167.1.

    Genome annotation databases

    GeneIDi1262586.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF069529 Genomic DNA. Translation: AAF31142.1 .
    RefSeqi NP_037749.1. NC_002167.1.

    3D structure databases

    ProteinModelPortali Q9MCN8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1262586.

    Family and domain databases

    Gene3Di 3.30.1330.70. 1 hit.
    InterProi IPR016281. Endonuclease_RusA.
    IPR008822. Endonuclease_RusA-like.
    [Graphical view ]
    Pfami PF05866. RusA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001007. RusA. 1 hit.
    SUPFAMi SSF103084. SSF103084. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages."
      Juhala R.J., Ford M.E., Duda R.L., Youlton A., Hatfull G.F., Hendrix R.W.
      J. Mol. Biol. 299:27-51(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiRUSA_BPHK7
    AccessioniPrimary (citable) accession number: Q9MCN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 29, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3