Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Crossover junction endodeoxyribonuclease rusA

Gene

rusA

Organism
Enterobacteria phage HK97 (Bacteriophage HK97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity.

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70MagnesiumBy similarity1
Metal bindingi72MagnesiumBy similarity1
Metal bindingi91MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease rusA (EC:3.1.22.4)
Alternative name(s):
Gp67
Holliday junction nuclease rusA
Holliday junction resolvase
Gene namesi
Name:rusA
Synonyms:67
OrganismiEnterobacteria phage HK97 (Bacteriophage HK97)
Taxonomic identifieri37554 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdavirus
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000002576 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001920071 – 120Crossover junction endodeoxyribonuclease rusAAdd BLAST120

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9MCN8.
SMRiQ9MCN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RusA family.Curated

Phylogenomic databases

KOiK01160.
OrthoDBiVOG0900013Y.

Family and domain databases

Gene3Di3.30.1330.70. 1 hit.
InterProiView protein in InterPro
IPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
PfamiView protein in Pfam
PF05866. RusA. 1 hit.
PIRSFiPIRSF001007. RusA. 1 hit.
SUPFAMiSSF103084. SSF103084. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9MCN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEYQFVLPY PPSLNTYWRR RGSQYYISDK GQKYRKDVQQ IIRQLKLDIF
60 70 80 90 100
TKSRLRIKVI ADVPDSRRRD LDNILKGLLD SLIHAGFAED DEQFDDIRVI
110 120
RGVKVPGGRL GIKITELESV
Length:120
Mass (Da):14,067
Last modified:October 1, 2000 - v1
Checksum:i7D28A53BA6ED7398
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069529 Genomic DNA. Translation: AAF31142.1.
RefSeqiNP_037749.1. NC_002167.1.

Genome annotation databases

GeneIDi1262586.
KEGGivg:1262586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069529 Genomic DNA. Translation: AAF31142.1.
RefSeqiNP_037749.1. NC_002167.1.

3D structure databases

ProteinModelPortaliQ9MCN8.
SMRiQ9MCN8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1262586.
KEGGivg:1262586.

Phylogenomic databases

KOiK01160.
OrthoDBiVOG0900013Y.

Family and domain databases

Gene3Di3.30.1330.70. 1 hit.
InterProiView protein in InterPro
IPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
PfamiView protein in Pfam
PF05866. RusA. 1 hit.
PIRSFiPIRSF001007. RusA. 1 hit.
SUPFAMiSSF103084. SSF103084. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUSA_BPHK7
AccessioniPrimary (citable) accession number: Q9MCN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 1, 2000
Last modified: March 15, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.