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Q9MCN8 (RUSA_BPHK7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease rusA

EC=3.1.22.4
Alternative name(s):
Gp67
Holliday junction nuclease rusA
Holliday junction resolvase
Gene names
Name:rusA
Synonyms:67
OrganismEnterobacteria phage HK97 (Bacteriophage HK97) [Complete proteome]
Taxonomic identifier37554 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesSiphoviridaeLambdalikevirus
Virus hostEscherichia coli [TaxID: 562]

Protein attributes

Sequence length120 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that resolves Holliday junction intermediates. Promotes DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity.

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the RusA family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 120120Crossover junction endodeoxyribonuclease rusA
PRO_0000192007

Sites

Metal binding701Magnesium By similarity
Metal binding721Magnesium By similarity
Metal binding911Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9MCN8 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 7D28A53BA6ED7398

FASTA12014,067
        10         20         30         40         50         60 
MNEYQFVLPY PPSLNTYWRR RGSQYYISDK GQKYRKDVQQ IIRQLKLDIF TKSRLRIKVI 

        70         80         90        100        110        120 
ADVPDSRRRD LDNILKGLLD SLIHAGFAED DEQFDDIRVI RGVKVPGGRL GIKITELESV 

« Hide

References

[1]"Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages."
Juhala R.J., Ford M.E., Duda R.L., Youlton A., Hatfull G.F., Hendrix R.W.
J. Mol. Biol. 299:27-51(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF069529 Genomic DNA. Translation: AAF31142.1.
RefSeqNP_037749.1. NC_002167.1.

3D structure databases

ProteinModelPortalQ9MCN8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1262586.

Phylogenomic databases

ProtClustDBCLSP987275.

Family and domain databases

Gene3D3.30.1330.70. 1 hit.
InterProIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamPF05866. RusA. 1 hit.
[Graphical view]
PIRSFPIRSF001007. RusA. 1 hit.
SUPFAMSSF103084. SSF103084. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUSA_BPHK7
AccessionPrimary (citable) accession number: Q9MCN8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families