Reviewed, UniProtKB/Swiss-Prot Q9MB35 (PERQ_SEDLI)

Names and origin

Protein names

Recommended name:
    Peroxiredoxin Q, chloroplastic
    EC=1.11.1.15
Alternative name(s):
    Thioredoxin reductase

Gene names

Name:

PRXQ

Organism

Sedum lineare (Needle stonecrop)

Taxonomic identifier

114260 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Saxifragales › Crassulaceae › Sedum

Protein attributes

Sequence length	186 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces hydrogen peroxide with reducing equivalents provided through the thioredoxin system By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Monomer. Ref.1
Subcellular location	Plastid › chloroplast thylakoid By similarity.
Post-translational modification	The Cys-80-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-80 (probably Cys-SOH) rapidly reacts with Cys-85-SH to form an intramolecular disulfide. This disulfide is subsequently reduced by thioredoxin to restore the reduced active form of the enzyme.
Sequence similarities	Belongs to the ahpC/TSA family. PrxQ subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 36	›36	Chloroplast Potential
	Chain	37 – 186	150	Peroxiredoxin Q, chloroplastic		PRO_5000049527
Regions
	Domain	38 – 186	149	Thioredoxin
Sites
	Active site	80	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	80 ↔ 85		Redox-active
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q9MB35-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 31E915D940CF2402	FASTA	186	20,652
	10 20 30 40 50 60 QTLQTSSQSQ FHGLKFSHAS SFKSPSAPLR KNSIFAKVTK GSTPPPFTLK DQEGRPVSLS 70 80 90 100 110 120 KFKGKPVVVY FYPADETPGC TKQACAFRDS YEKFKKAGAE VVGISGDSSE SHKAFAKKYK 130 140 150 160 170 180 LPFTLLSDEG NKVRKEWGVP SDLFGTLPGR ETYVLDKNGV VQLVYNNQFQ PEKHIDETLK LLQSLK

References

[1]	"A novel peroxiredoxin of the plant Sedum lineare is a homologue of Escherichia coli bacterioferritin co-migratory protein (Bcp)." Kong W., Shiota S., Shi Y., Nakayama H., Nakayama K. Biochem. J. 351:107-114(2000) [PubMed: 10998352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB037598 mRNA. Translation: BAA90524.1.
3D structure databases
HSSP	HSSP built from PDB template 1QQ2 based on UniProtKB Q63716.
ModBase	Search...
Protein family/group databases
PeroxiBase	4304. SlinPrxQ.
Enzyme and pathway databases
BRENDA	1.11.1.15. 291237.
Family and domain databases
InterPro	IPR000866. Alkyl_hydroperoxide_Rdtase. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00578. AhpC-TSA. 1 hit. [Graphical view]
ProDom	PD003679. Thioredoxin_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PERQ_SEDLI

Accession

Primary (citable) accession number: Q9MB35

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)