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Q9MB14 (PPO2_IPOBA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyphenol oxidase II, chloroplastic

Short name=PPO-II
EC=1.10.3.1
Alternative name(s):
Catechol oxidase II
Gene names
Name:co-2
Synonyms:ppo-I
OrganismIpomoea batatas (Sweet potato) (Convolvulus batatas)
Taxonomic identifier4120 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesConvolvulaceaeIpomoeeaeIpomoea

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the oxidation of mono- and o-diphenols to o-diquinones By similarity.

Catalytic activity

2 catechol + O2 = 2 1,2-benzoquinone + 2 H2O.

Cofactor

Binds 2 copper ions per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Plastidchloroplast thylakoid lumen By similarity.

Sequence similarities

Belongs to the tyrosinase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
Thylakoid
   DomainTransit peptide
   LigandCopper
Metal-binding
   Molecular functionOxidoreductase
   PTMDisulfide bond
Thioether bond
Gene Ontology (GO)
   Biological_processpigment biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast thylakoid lumen

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncatechol oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Chloroplast Potential
Transit peptide51 – 8838Thylakoid By similarity
Chain89 – 588500Polyphenol oxidase II, chloroplastic
PRO_0000035909

Sites

Metal binding1781Copper A By similarity
Metal binding1991Copper A By similarity
Metal binding2081Copper A By similarity
Metal binding3301Copper B By similarity
Metal binding3341Copper B By similarity
Metal binding3661Copper B By similarity

Amino acid modifications

Disulfide bond99 ↔ 116 By similarity
Disulfide bond115 ↔ 179 By similarity
Cross-link182 ↔ 1992'-(S-cysteinyl)-histidine (Cys-His) By similarity

Experimental info

Sequence conflict1301K → R in CAC29040. Ref.2
Sequence conflict2771T → N in CAC29040. Ref.2
Sequence conflict4931Q → E in BAA92317. Ref.1
Sequence conflict5631A → P in CAC83610. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9MB14 [UniParc].

Last modified March 29, 2005. Version 2.
Checksum: F0DF1A03FDC33AC2

FASTA58865,651
        10         20         30         40         50         60 
MASFTTSPCT SAAPKTPKSL SSSATISSPL PKPSQIHIAT AKRTHHFKVS CNAPNGDSQP 

        70         80         90        100        110        120 
KLDRRDVLLG LGGLAGAASL INNPLAFAEP IHAPEISKCV VPPKDLPPDA IVDNCCPPLA 

       130        140        150        160        170        180 
TNVIPYKVPK TSPSAMKIRP AIHRMDKEYI AKFEKAIRLM KELPADDPRN FYQQALVHCA 

       190        200        210        220        230        240 
YCNGGYVQTD YPDKEIQVHN SWLFFPFHRW YLYFYERILG KLIGDPTFGL PFWNWDTPAG 

       250        260        270        280        290        300 
MLIPQYFRNQ NSPLYDENRN QSHLPLVMDL GYAGTDTDVT DQERISNNLA LMYKSMVTNA 

       310        320        330        340        350        360 
GTAELFLGKP YKAGDDPVNK GGGSIENIPH TPVHRWVGDV KPRTQNGEDM GNFYSAGRDI 

       370        380        390        400        410        420 
LFYCHHSNVD RMWTIWQQLG GKGRRRDFTD SDWLDATFIF YDENKQAVRV RVGDALDNQK 

       430        440        450        460        470        480 
LGYKYEFTNL PWLNSKPLPT KKKTGLAARS KAPFVTDVFP LTLDKVVQVK VPRPKKSRSK 

       490        500        510        520        530        540 
EEKEAEEEIL EIQGIEVAID QYAKFDVYLN DEDEPEAGKE KAEYAGSFAH LPHKHTGSKK 

       550        560        570        580 
IRTSLSLGLN EPLEDLGAED DDAVLVTLAP KVGGGVVTVE NIKIVYGS 

« Hide

References

[1]"Ipomoea batatas polyphenol oxidase I mRNA."
Hashimoto H., Nozue M., Tanaka I.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and structural implications of two isozymes of Ipomoea batatas catechol oxidase differing in catalase activity."
Gerdemann C., Eicken C., Magrini A., Meier H.E., Spener F., Krebs B.
Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 89-588.
Strain: cv. Bushbuck.
Tissue: Root.
[3]"Sequencing and cloning of genomic DNA encoding two isozymes of Ipomoea batatas catechol oxidase."
Greving J., Gerdemann C., Spener F., Krebs B.
Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 89-588.
Strain: cv. Bushbuck.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038994 mRNA. Translation: BAA92317.1.
AJ245880 mRNA. Translation: CAC29040.1.
AJ309176 Genomic DNA. Translation: CAC83610.1.

3D structure databases

ProteinModelPortalQ9MB14.
SMRQ9MB14. Positions 90-433.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.1280.10. 1 hit.
InterProIPR016213. Polyphenol_oxidase.
IPR022740. Polyphenol_oxidase_C.
IPR022739. Polyphenol_oxidase_cen.
IPR002227. Tyrosinase.
IPR008922. Unchr_di-copper_centre.
[Graphical view]
PfamPF12142. PPO1_DWL. 1 hit.
PF12143. PPO1_KFDV. 1 hit.
PF00264. Tyrosinase. 1 hit.
[Graphical view]
PIRSFPIRSF000290. PPO_plant. 1 hit.
PRINTSPR00092. TYROSINASE.
SUPFAMSSF48056. SSF48056. 1 hit.
PROSITEPS00497. TYROSINASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPO2_IPOBA
AccessionPrimary (citable) accession number: Q9MB14
Secondary accession number(s): Q84V52, Q9ARD3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: February 19, 2014
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families