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Protein

3-ketoacyl-CoA synthase 1

Gene

KCS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Contributes to cuticular wax and suberin biosynthesis. Involved in both decarbonylation and acyl-reduction wax synthesis pathways. Elongase condensing enzyme mostly active with saturated fatty acids, especially with 16:0, 16:1, 18:0, and 20:0. Mediates the synthesis of VLCFAs from 20 to 26 carbons in length (e.g. C20:1, C20, C22, C24 and C26).3 Publications

Catalytic activityi

A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO2.2 Publications

Enzyme regulationi

Inhibited by K3 herbicides such as alachlor, allidochlor, anilofos, cafenstrole, fentrazamide and flufenacet (PubMed:15277688). Strongly inhibited by metazachlor and mefluidide (PubMed:22284369).2 Publications

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei258 – 2581By similarity
Active sitei337 – 3371By similarity
Active sitei421 – 4211By similarity
Active sitei425 – 4251By similarity
Active sitei454 – 4541By similarity
Active sitei458 – 4581By similarity

GO - Molecular functioni

  • fatty acid elongase activity Source: TAIR

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-UniPathway
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • wax biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BioCyciARA:AT1G01120-MONOMER.
MetaCyc:AT1G01120-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA synthase 11 Publication (EC:2.3.1.1992 Publications)
Short name:
KCS-11 Publication
Alternative name(s):
Very long-chain fatty acid condensing enzyme 11 Publication
Short name:
VLCFA condensing enzyme 11 Publication
Gene namesi
Name:KCS11 Publication
Synonyms:EL1
Ordered Locus Names:At1g01120Imported
ORF Names:T25K16.11Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G01120.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei59 – 7921HelicalSequence analysisAdd
BLAST
Transmembranei95 – 11521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytosolic ribosome Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants have thinner stems with an altered wax composition, and are more sensitive to dehydration.1 Publication

Chemistry

ChEMBLiCHEMBL2242739.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5285283-ketoacyl-CoA synthase 1PRO_0000249093Add
BLAST

Proteomic databases

PaxDbiQ9MAM3.
PRIDEiQ9MAM3.

Expressioni

Tissue specificityi

Ubiquitous (PubMed:10074711). Expressed in siliques, flowers, leaves and stems. Barely detected in roots (PubMed:18465198).2 Publications

Inductioni

Repressed by herbicides such as flufenacet and benfuresate (PubMed:12916765). Down-regulated by darkness and low temperature, and up-regulated by salt, drought and osmotic stress (PubMed:18465198).2 Publications

Gene expression databases

GenevisibleiQ9MAM3. AT.

Interactioni

Protein-protein interaction databases

BioGridi24630. 6 interactions.
IntActiQ9MAM3. 1 interaction.
MINTiMINT-8063290.
STRINGi3702.AT1G01120.1.

Structurei

3D structure databases

ProteinModelPortaliQ9MAM3.
SMRiQ9MAM3. Positions 167-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 403290FAESequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated
Contains 1 FAE (fatty acid elongase) domain.Sequence analysis

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9MAM3.
KOiK15397.
OMAiLCMKEAR.
PhylomeDBiQ9MAM3.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9MAM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERTNSIEMD RERLTAEMAF RDSSSAVIRI RRRLPDLLTS VKLKYVKLGL
60 70 80 90 100
HNSCNVTTIL FFLIILPLTG TVLVQLTGLT FDTFSELWSN QAVQLDTATR
110 120 130 140 150
LTCLVFLSFV LTLYVANRSK PVYLVDFSCY KPEDERKISV DSFLTMTEEN
160 170 180 190 200
GSFTDDTVQF QQRISNRAGL GDETYLPRGI TSTPPKLNMS EARAEAEAVM
210 220 230 240 250
FGALDSLFEK TGIKPAEVGI LIVNCSLFNP TPSLSAMIVN HYKMREDIKS
260 270 280 290 300
YNLGGMGCSA GLISIDLANN LLKANPNSYA VVVSTENITL NWYFGNDRSM
310 320 330 340 350
LLCNCIFRMG GAAILLSNRR QDRKKSKYSL VNVVRTHKGS DDKNYNCVYQ
360 370 380 390 400
KEDERGTIGV SLARELMSVA GDALKTNITT LGPMVLPLSE QLMFLISLVK
410 420 430 440 450
RKMFKLKVKP YIPDFKLAFE HFCIHAGGRA VLDEVQKNLD LKDWHMEPSR
460 470 480 490 500
MTLHRFGNTS SSSLWYEMAY TEAKGRVKAG DRLWQIAFGS GFKCNSAVWK
510 520
ALRPVSTEEM TGNAWAGSID QYPVKVVQ
Length:528
Mass (Da):59,276
Last modified:October 1, 2000 - v1
Checksum:iDEA6CBF18692A33B
GO

Sequence cautioni

The sequence BAD95286.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti467 – 4671E → G in BAD95286 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007323 Genomic DNA. Translation: AAF26470.1.
CP002684 Genomic DNA. Translation: AEE27241.1.
AY074285 mRNA. Translation: AAL66982.1.
AY096568 mRNA. Translation: AAM20218.1.
AF053345 Genomic DNA. Translation: AAC99312.1.
AK221191 mRNA. Translation: BAD95286.1. Different initiation.
PIRiF86141.
RefSeqiNP_171620.2. NM_099994.3.
UniGeneiAt.22667.

Genome annotation databases

EnsemblPlantsiAT1G01120.1; AT1G01120.1; AT1G01120.
GeneIDi839395.
GrameneiAT1G01120.1; AT1G01120.1; AT1G01120.
KEGGiath:AT1G01120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007323 Genomic DNA. Translation: AAF26470.1.
CP002684 Genomic DNA. Translation: AEE27241.1.
AY074285 mRNA. Translation: AAL66982.1.
AY096568 mRNA. Translation: AAM20218.1.
AF053345 Genomic DNA. Translation: AAC99312.1.
AK221191 mRNA. Translation: BAD95286.1. Different initiation.
PIRiF86141.
RefSeqiNP_171620.2. NM_099994.3.
UniGeneiAt.22667.

3D structure databases

ProteinModelPortaliQ9MAM3.
SMRiQ9MAM3. Positions 167-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24630. 6 interactions.
IntActiQ9MAM3. 1 interaction.
MINTiMINT-8063290.
STRINGi3702.AT1G01120.1.

Chemistry

ChEMBLiCHEMBL2242739.

Proteomic databases

PaxDbiQ9MAM3.
PRIDEiQ9MAM3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G01120.1; AT1G01120.1; AT1G01120.
GeneIDi839395.
GrameneiAT1G01120.1; AT1G01120.1; AT1G01120.
KEGGiath:AT1G01120.

Organism-specific databases

TAIRiAT1G01120.

Phylogenomic databases

eggNOGiENOG410IHMW. Eukaryota.
ENOG410Y5VH. LUCA.
HOGENOMiHOG000238893.
InParanoidiQ9MAM3.
KOiK15397.
OMAiLCMKEAR.
PhylomeDBiQ9MAM3.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciARA:AT1G01120-MONOMER.
MetaCyc:AT1G01120-MONOMER.

Miscellaneous databases

PROiQ9MAM3.

Gene expression databases

GenevisibleiQ9MAM3. AT.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012392. 3-ktacl-CoA_syn.
IPR013747. ACP_syn_III_C.
IPR013601. FAE1_typ3_polyketide_synth.
IPR016039. Thiolase-like.
[Graphical view]
PANTHERiPTHR31561. PTHR31561. 1 hit.
PfamiPF08541. ACP_syn_III_C. 1 hit.
PF08392. FAE1_CUT1_RppA. 1 hit.
[Graphical view]
PIRSFiPIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana."
    Todd J., Post-Beittenmiller D., Jaworski J.G.
    Plant J. 17:119-130(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-528, FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 400-528.
    Strain: cv. Columbia.
  6. "Flufenacet herbicide treatment phenocopies the fiddlehead mutant in Arabidopsis thaliana."
    Lechelt-Kunze C., Meissner R.C., Drewes M., Tietjen K.
    Pest Manag. Sci. 59:847-856(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, GENE FAMILY.
  7. "Specific and differential inhibition of very-long-chain fatty acid elongases from Arabidopsis thaliana by different herbicides."
    Trenkamp S., Martin W., Tietjen K.
    Proc. Natl. Acad. Sci. U.S.A. 101:11903-11908(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  8. "Substrate specificity of Arabidopsis 3-ketoacyl-CoA synthases."
    Blacklock B.J., Jaworski J.G.
    Biochem. Biophys. Res. Commun. 346:583-590(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
  9. "The VLCFA elongase gene family in Arabidopsis thaliana: phylogenetic analysis, 3D modelling and expression profiling."
    Joubes J., Raffaele S., Bourdenx B., Garcia C., Laroche-Traineau J., Moreau P., Domergue F., Lessire R.
    Plant Mol. Biol. 67:547-566(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, 3D-STRUCTURE MODELING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  10. "Inhibition of saturated very-long-chain fatty acid biosynthesis by mefluidide and perfluidone, selective inhibitors of 3-ketoacyl-CoA synthases."
    Tresch S., Heilmann M., Christiansen N., Looser R., Grossmann K.
    Phytochemistry 76:162-171(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.

Entry informationi

Entry nameiKCS1_ARATH
AccessioniPrimary (citable) accession number: Q9MAM3
Secondary accession number(s): Q56YX9, Q9ZTK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2000
Last modified: February 17, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.