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Protein

3-ketoacyl-CoA synthase 1

Gene

KCS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to cuticular wax and suberin biosynthesis. Involved in both decarbonylation and acyl-reduction wax synthesis pathways. Elongase condensing enzyme mostly active with saturated fatty acids, especially with 16:0, 16:1, 18:0, and 20:0. Mediates the synthesis of VLCFAs from 20 to 26 carbons in length (e.g. C20:1, C20, C22, C24 and C26).3 Publications

Caution

It is uncertain whether Met-1 or Met-9 is the initiator.Curated

Catalytic activityi

A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO2.2 Publications

Enzyme regulationi

Inhibited by K3 herbicides such as alachlor, allidochlor, anilofos, cafenstrole, fentrazamide and flufenacet (PubMed:15277688). Strongly inhibited by metazachlor and mefluidide (PubMed:22284369).2 Publications

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei258By similarity1
Active sitei337By similarity1
Active sitei421By similarity1
Active sitei425By similarity1
Active sitei454By similarity1
Active sitei458By similarity1

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-UniPathway
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • wax biosynthetic process Source: TAIR

Keywordsi

Molecular functionAcyltransferase, Transferase
LigandNAD, NADP

Enzyme and pathway databases

BioCyciARA:AT1G01120-MONOMER
MetaCyc:AT1G01120-MONOMER
UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA synthase 11 Publication (EC:2.3.1.1992 Publications)
Short name:
KCS-11 Publication
Alternative name(s):
Very long-chain fatty acid condensing enzyme 11 Publication
Short name:
VLCFA condensing enzyme 11 Publication
Gene namesi
Name:KCS11 Publication
Synonyms:EL1
Ordered Locus Names:At1g01120Imported
ORF Names:T25K16.11Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G01120
TAIRilocus:2200955 AT1G01120

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei59 – 79HelicalSequence analysisAdd BLAST21
Transmembranei95 – 115HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants have thinner stems with an altered wax composition, and are more sensitive to dehydration.1 Publication

Chemistry databases

ChEMBLiCHEMBL2242739

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002490931 – 5283-ketoacyl-CoA synthase 1Add BLAST528

Proteomic databases

PaxDbiQ9MAM3
PRIDEiQ9MAM3

PTM databases

iPTMnetiQ9MAM3

Expressioni

Tissue specificityi

Ubiquitous (PubMed:10074711). Expressed in siliques, flowers, leaves and stems. Barely detected in roots (PubMed:18465198).2 Publications

Inductioni

Repressed by herbicides such as flufenacet and benfuresate (PubMed:12916765). Down-regulated by darkness and low temperature, and up-regulated by salt, drought and osmotic stress (PubMed:18465198).2 Publications

Gene expression databases

ExpressionAtlasiQ9MAM3 baseline and differential
GenevisibleiQ9MAM3 AT

Interactioni

Protein-protein interaction databases

BioGridi24630, 7 interactors
IntActiQ9MAM3, 1 interactor
STRINGi3702.AT1G01120.1

Structurei

3D structure databases

ProteinModelPortaliQ9MAM3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 403FAESequence analysisAdd BLAST290

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHMW Eukaryota
ENOG410Y5VH LUCA
HOGENOMiHOG000238893
InParanoidiQ9MAM3
KOiK15397
OMAiPGIQARP
OrthoDBiEOG093606UZ
PhylomeDBiQ9MAM3

Family and domain databases

Gene3Di3.40.47.10, 1 hit
InterProiView protein in InterPro
IPR012392 3-ktacl-CoA_syn
IPR013747 ACP_syn_III_C
IPR013601 FAE1_typ3_polyketide_synth
IPR016039 Thiolase-like
PANTHERiPTHR31561 PTHR31561, 1 hit
PfamiView protein in Pfam
PF08541 ACP_syn_III_C, 1 hit
PF08392 FAE1_CUT1_RppA, 1 hit
PIRSFiPIRSF036417 3-ktacl-CoA_syn, 1 hit
SUPFAMiSSF53901 SSF53901, 2 hits

Sequencei

Sequence statusi: Complete.

Q9MAM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERTNSIEMD RERLTAEMAF RDSSSAVIRI RRRLPDLLTS VKLKYVKLGL
60 70 80 90 100
HNSCNVTTIL FFLIILPLTG TVLVQLTGLT FDTFSELWSN QAVQLDTATR
110 120 130 140 150
LTCLVFLSFV LTLYVANRSK PVYLVDFSCY KPEDERKISV DSFLTMTEEN
160 170 180 190 200
GSFTDDTVQF QQRISNRAGL GDETYLPRGI TSTPPKLNMS EARAEAEAVM
210 220 230 240 250
FGALDSLFEK TGIKPAEVGI LIVNCSLFNP TPSLSAMIVN HYKMREDIKS
260 270 280 290 300
YNLGGMGCSA GLISIDLANN LLKANPNSYA VVVSTENITL NWYFGNDRSM
310 320 330 340 350
LLCNCIFRMG GAAILLSNRR QDRKKSKYSL VNVVRTHKGS DDKNYNCVYQ
360 370 380 390 400
KEDERGTIGV SLARELMSVA GDALKTNITT LGPMVLPLSE QLMFLISLVK
410 420 430 440 450
RKMFKLKVKP YIPDFKLAFE HFCIHAGGRA VLDEVQKNLD LKDWHMEPSR
460 470 480 490 500
MTLHRFGNTS SSSLWYEMAY TEAKGRVKAG DRLWQIAFGS GFKCNSAVWK
510 520
ALRPVSTEEM TGNAWAGSID QYPVKVVQ
Length:528
Mass (Da):59,276
Last modified:October 1, 2000 - v1
Checksum:iDEA6CBF18692A33B
GO

Sequence cautioni

The sequence BAD95286 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti467E → G in BAD95286 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC007323 Genomic DNA Translation: AAF26470.1
CP002684 Genomic DNA Translation: AEE27241.1
AY074285 mRNA Translation: AAL66982.1
AY096568 mRNA Translation: AAM20218.1
AF053345 Genomic DNA Translation: AAC99312.1
AK221191 mRNA Translation: BAD95286.1 Different initiation.
PIRiF86141
RefSeqiNP_171620.2, NM_099994.4
UniGeneiAt.22667

Genome annotation databases

EnsemblPlantsiAT1G01120.1; AT1G01120.1; AT1G01120
GeneIDi839395
GrameneiAT1G01120.1; AT1G01120.1; AT1G01120
KEGGiath:AT1G01120

Similar proteinsi

Entry informationi

Entry nameiKCS1_ARATH
AccessioniPrimary (citable) accession number: Q9MAM3
Secondary accession number(s): Q56YX9, Q9ZTK3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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