Reviewed, UniProtKB/Swiss-Prot Q9MAM1 (CIPK9_ARATH)

Names and origin

Protein names

Recommended name:
    CBL-interacting serine/threonine-protein kinase 9
    EC=2.7.11.1
Alternative name(s):
    SOS2-like protein kinase PKS6
    SNF1-related kinase 3.12

Gene names

Name:	CIPK9
Synonyms:	PKS6, SnRK3.12
Ordered Locus Names:	At1g01140
ORF Names:	F6F3.28, T25K16.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Manganese By similarity.
Subunit structure	Interacts with CBL2. Ref.1
Tissue specificity	Expressed at low levels in roots and shoots. Ref.2
Domain	The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 NAF domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Manganese Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 (identifier: Q9MAM1-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 1 (identifier: Q9MAM1-1)

The sequence of this isoform differs from the canonical sequence as follows:
     231-231: R → RVR
     422-422: K → KVCDS

Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

Isoform 2 (identifier: Q9MAM1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     231-231: R → RVR

Note: May be due to a competing donor splice site. No experimental confirmation available.

Isoform 4 (identifier: Q9MAM1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     422-422: K → KVCDS

Note: May be due to a competing donor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	CBL-interacting serine/threonine-protein kinase 9		PRO_0000337212
Regions
	Domain	19 – 274	256	Protein kinase
	Domain	312 – 336	25	NAF
	Nucleotide binding	25 – 33	9	ATP By similarity
	Region	160 – 189	30	Activation loop By similarity
	Region	343 – 372	30	PPI By similarity
Sites
	Active site	142	1	Proton acceptor By similarity
	Binding site	48	1	ATP By similarity
Natural variations
	Alternative sequence	231	1	R → RVR in isoform 1 and isoform 2.		VSP_033983
	Alternative sequence	422	1	K → KVCDS in isoform 1 and isoform 4.		VSP_033984
Experimental info
	Sequence conflict	33	1	V → A Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 5E1A9B8DD37A0E32	FASTA	447	50,505
	10 20 30 40 50 60 MSGSRRKATP ASRTRVGNYE MGRTLGEGSF AKVKYAKNTV TGDQAAIKIL DREKVFRHKM 70 80 90 100 110 120 VEQLKREIST MKLIKHPNVV EIIEVMASKT KIYIVLELVN GGELFDKIAQ QGRLKEDEAR 130 140 150 160 170 180 RYFQQLINAV DYCHSRGVYH RDLKPENLIL DANGVLKVSD FGLSAFSRQV REDGLLHTAC 190 200 210 220 230 240 GTPNYVAPEV LSDKGYDGAA ADVWSCGVIL FVLMAGYLPF DEPNLMTLYK RICKAEFSCP 250 260 270 280 290 300 PWFSQGAKRV IKRILEPNPI TRISIAELLE DEWFKKGYKP PSFDQDDEDI TIDDVDAAFS 310 320 330 340 350 360 NSKECLVTEK KEKPVSMNAF ELISSSSEFS LENLFEKQAQ LVKKETRFTS QRSASEIMSK 370 380 390 400 410 420 MEETAKPLGF NVRKDNYKIK MKGDKSGRKG QLSVATEVFE VAPSLHVVEL RKTGGDTLEF 430 440 HKFYKNFSSG LKDVVWNTDA AAEEQKQ
	Isoform 1. Checksum: 544789BF24758285	FASTA	453	51,165
	10 20 30 40 50 60 MSGSRRKATP ASRTRVGNYE MGRTLGEGSF AKVKYAKNTV TGDQAAIKIL DREKVFRHKM 70 80 90 100 110 120 VEQLKREIST MKLIKHPNVV EIIEVMASKT KIYIVLELVN GGELFDKIAQ QGRLKEDEAR 130 140 150 160 170 180 RYFQQLINAV DYCHSRGVYH RDLKPENLIL DANGVLKVSD FGLSAFSRQV REDGLLHTAC 190 200 210 220 230 240 GTPNYVAPEV LSDKGYDGAA ADVWSCGVIL FVLMAGYLPF DEPNLMTLYK RVRICKAEFS 250 260 270 280 290 300 CPPWFSQGAK RVIKRILEPN PITRISIAEL LEDEWFKKGY KPPSFDQDDE DITIDDVDAA 310 320 330 340 350 360 FSNSKECLVT EKKEKPVSMN AFELISSSSE FSLENLFEKQ AQLVKKETRF TSQRSASEIM 370 380 390 400 410 420 SKMEETAKPL GFNVRKDNYK IKMKGDKSGR KGQLSVATEV FEVAPSLHVV ELRKTGGDTL 430 440 450 EFHKVCDSFY KNFSSGLKDV VWNTDAAAEE QKQ
	Isoform 2. Checksum: BF3A98AB67AB2AFB	FASTA	449	50,760
	10 20 30 40 50 60 MSGSRRKATP ASRTRVGNYE MGRTLGEGSF AKVKYAKNTV TGDQAAIKIL DREKVFRHKM 70 80 90 100 110 120 VEQLKREIST MKLIKHPNVV EIIEVMASKT KIYIVLELVN GGELFDKIAQ QGRLKEDEAR 130 140 150 160 170 180 RYFQQLINAV DYCHSRGVYH RDLKPENLIL DANGVLKVSD FGLSAFSRQV REDGLLHTAC 190 200 210 220 230 240 GTPNYVAPEV LSDKGYDGAA ADVWSCGVIL FVLMAGYLPF DEPNLMTLYK RVRICKAEFS 250 260 270 280 290 300 CPPWFSQGAK RVIKRILEPN PITRISIAEL LEDEWFKKGY KPPSFDQDDE DITIDDVDAA 310 320 330 340 350 360 FSNSKECLVT EKKEKPVSMN AFELISSSSE FSLENLFEKQ AQLVKKETRF TSQRSASEIM 370 380 390 400 410 420 SKMEETAKPL GFNVRKDNYK IKMKGDKSGR KGQLSVATEV FEVAPSLHVV ELRKTGGDTL 430 440 EFHKFYKNFS SGLKDVVWNT DAAAEEQKQ
	Isoform 4. Checksum: A72909C7F55581A3	FASTA	451	50,909
	10 20 30 40 50 60 MSGSRRKATP ASRTRVGNYE MGRTLGEGSF AKVKYAKNTV TGDQAAIKIL DREKVFRHKM 70 80 90 100 110 120 VEQLKREIST MKLIKHPNVV EIIEVMASKT KIYIVLELVN GGELFDKIAQ QGRLKEDEAR 130 140 150 160 170 180 RYFQQLINAV DYCHSRGVYH RDLKPENLIL DANGVLKVSD FGLSAFSRQV REDGLLHTAC 190 200 210 220 230 240 GTPNYVAPEV LSDKGYDGAA ADVWSCGVIL FVLMAGYLPF DEPNLMTLYK RICKAEFSCP 250 260 270 280 290 300 PWFSQGAKRV IKRILEPNPI TRISIAELLE DEWFKKGYKP PSFDQDDEDI TIDDVDAAFS 310 320 330 340 350 360 NSKECLVTEK KEKPVSMNAF ELISSSSEFS LENLFEKQAQ LVKKETRFTS QRSASEIMSK 370 380 390 400 410 420 MEETAKPLGF NVRKDNYKIK MKGDKSGRKG QLSVATEVFE VAPSLHVVEL RKTGGDTLEF 430 440 450 HKVCDSFYKN FSSGLKDVVW NTDAAAEEQK Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The NAF domain defines a novel protein-protein interaction module conserved in Ca(2+)-regulated kinases." Albrecht V., Ritz O., Linder S., Harter K., Kudla J. EMBO J. 20:1051-1063(2001) [PubMed: 11230129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH CBL2.
[2]	"Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance." Guo Y., Halfter U., Ishitani M., Zhu J.-K. Plant Cell 13:1383-1400(2001) [PubMed: 11402167] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), TISSUE SPECIFICITY. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: cv. Columbia.
[5]	"The Arabidopsis CDPK-SnRK superfamily of protein kinases." Hrabak E.M., Chan C.W.M., Gribskov M., Harper J.F., Choi J.H., Halford N., Kudla J., Luan S., Nimmo H.G., Sussman M.R., Thomas M., Walker-Simmons K., Zhu J.-K., Harmon A.C. Plant Physiol. 132:666-680(2003) [PubMed: 12805596] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF295664 mRNA. Translation: AAK16684.1. AF339147 mRNA. Translation: AAK26845.1. AC007323 Genomic DNA. Translation: AAF26468.1. AF386999 mRNA. Translation: AAK62444.1. AY093242 mRNA. Translation: AAM13241.1.
IPI	IPI00534947. IPI00541707. IPI00544188. IPI00895662.
PIR	G86141.
RefSeq	NP_171622.1. NP_849570.1. NP_849571.1.
UniGene	At.11899
3D structure databases
HSSP	HSSP built from PDB template 1NXK based on UniProtKB P49137.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9MAM1. 8 interactions.
STRING	Q9MAM1.
Proteomic databases
PRIDE	Q9MAM1.
Genome annotation databases
GeneID	839349.
GenomeReviews	Gene locus AT1G01140 in contig CT485782_GR.
KEGG	ath:AT1G01140.
Organism-specific databases
TAIR	At1g01140.
Phylogenomic databases
OMA	RKTGGDT
Gene expression databases
Genevestigator	Q9MAM1.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR020660. CBL-interact_prot_kinase. IPR011009. Kinase-like_dom. IPR018451. NAF/FISL_domain. IPR004041. NAF_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF45. CBL-interact_prot_kinase. 1 hit.
Pfam	PF03822. NAF. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS50816. NAF. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CIPK9_ARATH

Accession

Primary (citable) accession number: Q9MAM1
Secondary accession number(s): Q3EDL5 , Q94F03, Q9C5P5, Q9C5S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 22, 2009
Last modified:	November 24, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)