Q9MAL0 (PME57_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative pectinesterase 57
Short name=PE 57
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase 57
Short name=AtPME57

Gene names

Name:	PME57
Synonyms:	ARATH57
Ordered Locus Names:	At1g44980
ORF Names:	F27F5.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Sequence similarities	Belongs to the pectinesterase family.
Sequence caution	The sequence AAF69174.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall modification Inferred from electronic annotation. Source: InterPro pectin catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cell wall Inferred from electronic annotation. Source: InterPro
Molecular_function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Putative pectinesterase 57		PRO_0000371705
Sites
	Active site	205	1	Proton donor By similarity
	Active site	226	1	Nucleophile By similarity
	Binding site	152	1	Substrate By similarity
	Site	204	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
	Glycosylation	143	1	N-linked (GlcNAc...) Potential
	Glycosylation	174	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9MAL0 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 278E69BBC60B7B39	FASTA	246	27,743
	10 20 30 40 50 60 MAMIGVMEDS KLHEDMENDM LGDLTSRARE ALALFISISL RDNTELNLVV PNGPSWLSHV 70 80 90 100 110 120 DKKDLYLNDE TLKKITDILV AKDVTGNYNI VNVAIAAAPQ HSQKRFVIYI KTSIYVEIVV 130 140 150 160 170 180 IGNMKSNLTL IADGQDSTII TFNLSSSNSK RTFNTATFAS NGDGFIRVDM CFRNTTWPVK 190 200 210 220 230 240 GPVVTLRVNG DMSIIYRCRV EEYQDALYPH KNRQCYREYF LMDTVDFICG NAAAVFQFCQ IVHMDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC007915 Genomic DNA. Translation: AAF69174.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE32070.1.
IPI	IPI00518651.
PIR	H96508.
RefSeq	NP_175118.2. NM_103578.2.
UniGene	At.52037.
3D structure databases
HSSP	HSSP built from PDB template 1XG2 based on UniProtKB P14280.
ProteinModelPortal	Q9MAL0.
SMR	Q9MAL0. Positions 75-242.
ModBase	Search...
Proteomic databases
PRIDE	Q9MAL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G44980.1; AT1G44980.1; AT1G44980.
GeneID	841063.
KEGG	ath:AT1G44980.
Organism-specific databases
TAIR	At1g44980.
Phylogenomic databases
eggNOG	COG4677.
OMA	MYEENIT.
PhylomeDB	Q9MAL0.
Enzyme and pathway databases
UniPathway	UPA00545; UER00823.
Gene expression databases
Genevestigator	Q9MAL0.
Family and domain databases
Gene3D	2.160.20.10. 1 hit.
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR000070. Pectinesterase_cat. [Graphical view]
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME57_ARATH

Accession

Primary (citable) accession number: Q9MAL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program