Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase 5

Gene

BGAL5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton donorSequence Analysis
Active sitei256 – 2561NucleophileSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G45130-MONOMER.
BRENDAi3.2.1.23. 399.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 5 (EC:3.2.1.23)
Short name:
Lactase 5
Gene namesi
Name:BGAL5
Ordered Locus Names:At1g45130
ORF Names:F27F5.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G45130.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 732709Beta-galactosidase 5PRO_5000065880Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9MAJ7.
PRIDEiQ9MAJ7.

Expressioni

Tissue specificityi

Expressed in leaves and flowers.1 Publication

Gene expression databases

GenevestigatoriQ9MAJ7.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G45130.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9MAJ7.
SMRiQ9MAJ7. Positions 30-730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9MAJ7.
OMAiNSNSYAK.
PhylomeDBiQ9MAJ7.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9MAJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTTILVLSK ILTFLLTTML IGSSVIQCSS VTYDKKAIVI NGHRRILLSG
60 70 80 90 100
SIHYPRSTPE MWEDLIKKAK DGGLDVIDTY VFWNGHEPSP GTYNFEGRYD
110 120 130 140 150
LVRFIKTIQE VGLYVHLRIG PYVCAEWNFG GFPVWLKYVD GISFRTDNGP
160 170 180 190 200
FKSAMQGFTE KIVQMMKEHR FFASQGGPII LSQIENEFEP DLKGLGPAGH
210 220 230 240 250
SYVNWAAKMA VGLNTGVPWV MCKEDDAPDP IINTCNGFYC DYFTPNKPYK
260 270 280 290 300
PTMWTEAWSG WFTEFGGTVP KRPVEDLAFG VARFIQKGGS YINYYMYHGG
310 320 330 340 350
TNFGRTAGGP FITTSYDYDA PIDEYGLVQE PKYSHLKQLH QAIKQCEAAL
360 370 380 390 400
VSSDPHVTKL GNYEEAHVFT AGKGSCVAFL TNYHMNAPAK VVFNNRHYTL
410 420 430 440 450
PAWSISILPD CRNVVFNTAT VAAKTSHVQM VPSGSILYSV ARYDEDIATY
460 470 480 490 500
GNRGTITARG LLEQVNVTRD TTDYLWYTTS VDIKASESFL RGGKWPTLTV
510 520 530 540 550
DSAGHAVHVF VNGHFYGSAF GTRENRKFSF SSQVNLRGGA NKIALLSVAV
560 570 580 590 600
GLPNVGPHFE TWATGIVGSV VLHGLDEGNK DLSWQKWTYQ AGLRGESMNL
610 620 630 640 650
VSPTEDSSVD WIKGSLAKQN KQPLTWYKAY FDAPRGNEPL ALDLKSMGKG
660 670 680 690 700
QAWINGQSIG RYWMAFAKGD CGSCNYAGTY RQNKCQSGCG EPTQRWYHVP
710 720 730
RSWLKPKGNL LVLFEELGGD ISKVSVVKRS VN
Length:732
Mass (Da):81,444
Last modified:October 1, 2000 - v1
Checksum:i0442C83D04F7CBC4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti453 – 4531R → P in CAB64741 (Ref. 1) Curated
Sequence conflicti571 – 5711V → A in CAB64741 (Ref. 1) Curated
Sequence conflicti633 – 6331A → V in AAL24206 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270301 mRNA. Translation: CAB64741.1.
AC007915 Genomic DNA. Translation: AAF69162.1.
CP002684 Genomic DNA. Translation: AEE32082.1.
AY058098 mRNA. Translation: AAL24206.1.
AY069911 mRNA. Translation: AAL47461.1.
AY093977 mRNA. Translation: AAM16238.1.
RefSeqiNP_175127.1. NM_103587.2.
UniGeneiAt.24850.
At.69432.

Genome annotation databases

EnsemblPlantsiAT1G45130.1; AT1G45130.1; AT1G45130.
GeneIDi841080.
KEGGiath:AT1G45130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ270301 mRNA. Translation: CAB64741.1.
AC007915 Genomic DNA. Translation: AAF69162.1.
CP002684 Genomic DNA. Translation: AEE32082.1.
AY058098 mRNA. Translation: AAL24206.1.
AY069911 mRNA. Translation: AAL47461.1.
AY093977 mRNA. Translation: AAM16238.1.
RefSeqiNP_175127.1. NM_103587.2.
UniGeneiAt.24850.
At.69432.

3D structure databases

ProteinModelPortaliQ9MAJ7.
SMRiQ9MAJ7. Positions 30-730.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G45130.1-P.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbiQ9MAJ7.
PRIDEiQ9MAJ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G45130.1; AT1G45130.1; AT1G45130.
GeneIDi841080.
KEGGiath:AT1G45130.

Organism-specific databases

GeneFarmi494. 90.
TAIRiAT1G45130.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000239919.
InParanoidiQ9MAJ7.
OMAiNSNSYAK.
PhylomeDBiQ9MAJ7.

Enzyme and pathway databases

BioCyciARA:AT1G45130-MONOMER.
BRENDAi3.2.1.23. 399.

Miscellaneous databases

PROiQ9MAJ7.

Gene expression databases

GenevestigatoriQ9MAJ7.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProiIPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
    Gy I., Kreis M., Lecharny A.
    Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
    Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
    Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGAL5_ARATH
AccessioniPrimary (citable) accession number: Q9MAJ7
Secondary accession number(s): Q93Z63, Q9SCV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2000
Last modified: April 29, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.