Q9MAJ7 (BGAL5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase 5
Short name=Lactase 5
EC=3.2.1.23

Gene names

Name:	BGAL5
Ordered Locus Names:	At1g45130
ORF Names:	F27F5.20

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Secreted › extracellular space › apoplast Potential.
Tissue specificity	Expressed in leaves and flowers. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Cellular component	Apoplast Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 732	709	Beta-galactosidase 5		PRO_5000065880
Sites
	Active site	187	1	Proton donor Potential
	Active site	256	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	453	1	R → P in CAB64741. Ref.1
	Sequence conflict	571	1	V → A in CAB64741. Ref.1
	Sequence conflict	633	1	A → V in AAL24206. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9MAJ7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 0442C83D04F7CBC4	FASTA	732	81,444
	10 20 30 40 50 60 MGTTILVLSK ILTFLLTTML IGSSVIQCSS VTYDKKAIVI NGHRRILLSG SIHYPRSTPE 70 80 90 100 110 120 MWEDLIKKAK DGGLDVIDTY VFWNGHEPSP GTYNFEGRYD LVRFIKTIQE VGLYVHLRIG 130 140 150 160 170 180 PYVCAEWNFG GFPVWLKYVD GISFRTDNGP FKSAMQGFTE KIVQMMKEHR FFASQGGPII 190 200 210 220 230 240 LSQIENEFEP DLKGLGPAGH SYVNWAAKMA VGLNTGVPWV MCKEDDAPDP IINTCNGFYC 250 260 270 280 290 300 DYFTPNKPYK PTMWTEAWSG WFTEFGGTVP KRPVEDLAFG VARFIQKGGS YINYYMYHGG 310 320 330 340 350 360 TNFGRTAGGP FITTSYDYDA PIDEYGLVQE PKYSHLKQLH QAIKQCEAAL VSSDPHVTKL 370 380 390 400 410 420 GNYEEAHVFT AGKGSCVAFL TNYHMNAPAK VVFNNRHYTL PAWSISILPD CRNVVFNTAT 430 440 450 460 470 480 VAAKTSHVQM VPSGSILYSV ARYDEDIATY GNRGTITARG LLEQVNVTRD TTDYLWYTTS 490 500 510 520 530 540 VDIKASESFL RGGKWPTLTV DSAGHAVHVF VNGHFYGSAF GTRENRKFSF SSQVNLRGGA 550 560 570 580 590 600 NKIALLSVAV GLPNVGPHFE TWATGIVGSV VLHGLDEGNK DLSWQKWTYQ AGLRGESMNL 610 620 630 640 650 660 VSPTEDSSVD WIKGSLAKQN KQPLTWYKAY FDAPRGNEPL ALDLKSMGKG QAWINGQSIG 670 680 690 700 710 720 RYWMAFAKGD CGSCNYAGTY RQNKCQSGCG EPTQRWYHVP RSWLKPKGNL LVLFEELGGD 730 ISKVSVVKRS VN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana." Gy I., Kreis M., Lecharny A. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35." Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C. Phytochemistry 68:1510-1520(2007) [PubMed: 17466346] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ270301 mRNA. Translation: CAB64741.1. AC007915 Genomic DNA. Translation: AAF69162.1. CP002684 Genomic DNA. Translation: AEE32082.1. AY058098 mRNA. Translation: AAL24206.1. AY069911 mRNA. Translation: AAL47461.1. AY093977 mRNA. Translation: AAM16238.1.
IPI	IPI00523074.
RefSeq	NP_175127.1. NM_103587.2.
UniGene	At.24850. At.69432.
3D structure databases
ProteinModelPortal	Q9MAJ7.
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Proteomic databases
PRIDE	Q9MAJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G45130.1; AT1G45130.1; AT1G45130.
GeneID	841080.
GenomeReviews	Gene locus AT1G45130 in contig CT485782_GR.
KEGG	ath:AT1G45130.
NMPDR	fig|3702.1.peg.4140.
Organism-specific databases
GeneFarm	494. 90.
TAIR	At1g45130.
Phylogenomic databases
eggNOG	KOG0496.
GeneTree	EPGT00070000028830.
HOGENOM	HBG318019.
InParanoid	Q9MAJ7.
OMA	IGRYWMA.
PhylomeDB	Q9MAJ7.
ProtClustDB	CLSN2914351.
Gene expression databases
Genevestigator	Q9MAJ7.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR006104. Glyco_hydro_2_N. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF02837. Glyco_hydro_2_N. 1 hit. PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL5_ARATH

Accession

Primary (citable) accession number: Q9MAJ7
Secondary accession number(s): Q93Z63, Q9SCV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 1, 2000
Last modified:	September 21, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program