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Q9MAJ7 (BGAL5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase 5

Short name=Lactase 5
EC=3.2.1.23
Gene names
Name:BGAL5
Ordered Locus Names:At1g45130
ORF Names:F27F5.20
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length732 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secretedextracellular spaceapoplast Potential.

Tissue specificity

Expressed in leaves and flowers. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
   Cellular componentApoplast
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 732709Beta-galactosidase 5
PRO_5000065880

Sites

Active site1871Proton donor Potential
Active site2561Nucleophile Potential

Amino acid modifications

Glycosylation4661N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4531R → P in CAB64741. Ref.1
Sequence conflict5711V → A in CAB64741. Ref.1
Sequence conflict6331A → V in AAL24206. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9MAJ7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 0442C83D04F7CBC4

FASTA73281,444
        10         20         30         40         50         60 
MGTTILVLSK ILTFLLTTML IGSSVIQCSS VTYDKKAIVI NGHRRILLSG SIHYPRSTPE 

        70         80         90        100        110        120 
MWEDLIKKAK DGGLDVIDTY VFWNGHEPSP GTYNFEGRYD LVRFIKTIQE VGLYVHLRIG 

       130        140        150        160        170        180 
PYVCAEWNFG GFPVWLKYVD GISFRTDNGP FKSAMQGFTE KIVQMMKEHR FFASQGGPII 

       190        200        210        220        230        240 
LSQIENEFEP DLKGLGPAGH SYVNWAAKMA VGLNTGVPWV MCKEDDAPDP IINTCNGFYC 

       250        260        270        280        290        300 
DYFTPNKPYK PTMWTEAWSG WFTEFGGTVP KRPVEDLAFG VARFIQKGGS YINYYMYHGG 

       310        320        330        340        350        360 
TNFGRTAGGP FITTSYDYDA PIDEYGLVQE PKYSHLKQLH QAIKQCEAAL VSSDPHVTKL 

       370        380        390        400        410        420 
GNYEEAHVFT AGKGSCVAFL TNYHMNAPAK VVFNNRHYTL PAWSISILPD CRNVVFNTAT 

       430        440        450        460        470        480 
VAAKTSHVQM VPSGSILYSV ARYDEDIATY GNRGTITARG LLEQVNVTRD TTDYLWYTTS 

       490        500        510        520        530        540 
VDIKASESFL RGGKWPTLTV DSAGHAVHVF VNGHFYGSAF GTRENRKFSF SSQVNLRGGA 

       550        560        570        580        590        600 
NKIALLSVAV GLPNVGPHFE TWATGIVGSV VLHGLDEGNK DLSWQKWTYQ AGLRGESMNL 

       610        620        630        640        650        660 
VSPTEDSSVD WIKGSLAKQN KQPLTWYKAY FDAPRGNEPL ALDLKSMGKG QAWINGQSIG 

       670        680        690        700        710        720 
RYWMAFAKGD CGSCNYAGTY RQNKCQSGCG EPTQRWYHVP RSWLKPKGNL LVLFEELGGD 

       730 
ISKVSVVKRS VN 

« Hide

References

« Hide 'large scale' references
[1]"The beta-galactosidases are encoding by a multigene family in Arabidopsis thaliana."
Gy I., Kreis M., Lecharny A.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 35."
Ahn Y.O., Zheng M., Bevan D.R., Esen A., Shiu S.-H., Benson J., Peng H.-P., Miller J.T., Cheng C.-L., Poulton J.E., Shih M.-C.
Phytochemistry 68:1510-1520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ270301 mRNA. Translation: CAB64741.1.
AC007915 Genomic DNA. Translation: AAF69162.1.
CP002684 Genomic DNA. Translation: AEE32082.1.
AY058098 mRNA. Translation: AAL24206.1.
AY069911 mRNA. Translation: AAL47461.1.
AY093977 mRNA. Translation: AAM16238.1.
RefSeqNP_175127.1. NM_103587.2.
UniGeneAt.24850.
At.69432.

3D structure databases

ProteinModelPortalQ9MAJ7.
SMRQ9MAJ7. Positions 30-730.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G45130.1-P.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PaxDbQ9MAJ7.
PRIDEQ9MAJ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G45130.1; AT1G45130.1; AT1G45130.
GeneID841080.
KEGGath:AT1G45130.

Organism-specific databases

GeneFarm494. 90.
TAIRAT1G45130.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000239919.
InParanoidQ9MAJ7.
OMATYDKKAI.
PhylomeDBQ9MAJ7.

Enzyme and pathway databases

BioCycARA:AT1G45130-MONOMER.

Gene expression databases

GenevestigatorQ9MAJ7.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
3.20.20.80. 1 hit.
InterProIPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SUPFAMSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL5_ARATH
AccessionPrimary (citable) accession number: Q9MAJ7
Secondary accession number(s): Q93Z63, Q9SCV7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names