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Protein

Phosphoenolpyruvate carboxylase 1

Gene

PPC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. Contributes probably to the adaptation to inorganic phosphate (Pi) deprivation.1 Publication

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Magnesium. Can also use manganese.1 Publication

Enzyme regulationi

By light-reversible phosphorylation (By similarity). Activated by inorganic phosphate (Pi) deprivation and glucose 6-phosphate. Inhibited by L-malate and L-aspartate.By similarity1 Publication

Kineticsi

  1. KM=0.18 mM for PEP (Phospho-PPC1 at pH 7.3 and 25 degrees Celsius)1 Publication
  2. KM=0.34 mM for PEP (Dephospho-PPC1 at pH 7.3 and 25 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 8-9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei173By similarity1
    Active sitei602By similarity1

    GO - Molecular functioni

    • phosphoenolpyruvate carboxylase activity Source: TAIR

    GO - Biological processi

    • carbon fixation Source: UniProtKB-KW
    • cellular response to phosphate starvation Source: UniProtKB
    • leaf development Source: TAIR
    • photosynthesis Source: UniProtKB-KW
    • protein tetramerization Source: UniProtKB
    • tricarboxylic acid cycle Source: InterPro

    Keywordsi

    Molecular functionAllosteric enzyme, Lyase
    Biological processCarbon dioxide fixation, Photosynthesis
    LigandMagnesium, Manganese

    Enzyme and pathway databases

    BRENDAi4.1.1.31 399
    SABIO-RKiQ9MAH0

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylase 1 (EC:4.1.1.31)
    Short name:
    AtPPC1
    Short name:
    PEPC 1
    Short name:
    PEPCase 1
    Alternative name(s):
    107-kDa PEPC polypeptide
    Gene namesi
    Name:PPC1
    Synonyms:p107
    Ordered Locus Names:At1g53310
    ORF Names:F12M16.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G53310
    TAIRilocus:2009600 AT1G53310

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001666571 – 967Phosphoenolpyruvate carboxylase 1Add BLAST967

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei11PhosphoserineCombined sources1 Publication1
    Modified residuei704PhosphoserineCombined sources1

    Post-translational modificationi

    The phosphorylation of Ser-11 is reversibly promoted by inorganic phosphate (Pi) deprivation. Enhanced activity by phosphorylation at pH 7.3 by lowering Km and sensitivity to inhibition by L-malate and L-aspartate, while enhancing activation by glucose 6-phosphate.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9MAH0
    PRIDEiQ9MAH0

    PTM databases

    iPTMnetiQ9MAH0

    Expressioni

    Tissue specificityi

    Expressed in all plant organs, with higher levels in roots.2 Publications

    Inductioni

    Upon inorganic phosphate (Pi) deprivation.1 Publication

    Gene expression databases

    ExpressionAtlasiQ9MAH0 baseline and differential
    GenevisibleiQ9MAH0 AT

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi26990, 6 interactors
    IntActiQ9MAH0, 2 interactors
    STRINGi3702.AT1G53310.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9MAH0
    SMRiQ9MAH0
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 1 family.Curated

    Phylogenomic databases

    eggNOGiENOG410IEAR Eukaryota
    COG2352 LUCA
    HOGENOMiHOG000238648
    InParanoidiQ9MAH0
    KOiK01595
    OMAiTTHLDIG
    OrthoDBiEOG0936013M
    PhylomeDBiQ9MAH0

    Family and domain databases

    HAMAPiMF_00595 PEPcase_type1, 1 hit
    InterProiView protein in InterPro
    IPR021135 PEP_COase
    IPR022805 PEP_COase_bac/pln-type
    IPR018129 PEP_COase_Lys_AS
    IPR033129 PEPCASE_His_AS
    IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
    PANTHERiPTHR30523 PTHR30523, 1 hit
    PfamiView protein in Pfam
    PF00311 PEPcase, 1 hit
    PRINTSiPR00150 PEPCARBXLASE
    SUPFAMiSSF51621 SSF51621, 1 hit
    PROSITEiView protein in PROSITE
    PS00781 PEPCASE_1, 1 hit
    PS00393 PEPCASE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9MAH0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MANRKLEKMA SIDVHLRQLV PGKVSEDDKL VEYDALLLDR FLDILQDLHG
    60 70 80 90 100
    EDLRETVQEL YEHSAEYEGK HEPKKLEELG SVLTSLDPGD SIVIAKAFSH
    110 120 130 140 150
    MLNLANLAEE VQIAYRRRIK KLKKGDFVDE SSATTESDLE ETFKKLVGDL
    160 170 180 190 200
    NKSPEEIFDA LKNQTVDLVL TAHPTQSVRR SLLQKHGRIR DCLAQLYAKD
    210 220 230 240 250
    ITPDDKQELD EALQREIQAA FRTDEIKRTP PTPQDEMRAG MSYFHETIWK
    260 270 280 290 300
    GVPKFLRRVD TALKNIGIEE RVPYNAPLIQ FSSWMGGDRD GNPRVTPEVT
    310 320 330 340 350
    RDVCLLARMM AATMYFNQIE DLMFEMSMWR CNDELRARAD EVHANSRKDA
    360 370 380 390 400
    AKHYIEFWKS IPTTEPYRVI LGDVRDKLYH TRERAHQLLS NGHSDVPVEA
    410 420 430 440 450
    TFINLEQFLE PLELCYRSLC SCGDRPIADG SLLDFLRQVS TFGLSLVRLD
    460 470 480 490 500
    IRQESDRHTD VLDAITTHLD IGSYREWSEE RRQEWLLSEL SGKRPLFGSD
    510 520 530 540 550
    LPKTEEIADV LDTFHVIAEL PADSFGAYII SMATAPSDVL AVELLQRECR
    560 570 580 590 600
    VKQPLRVVPL FEKLADLEAA PAAVARLFSV DWYKNRINGK QEVMIGYSDS
    610 620 630 640 650
    GKDAGRLSAA WQLYKAQEEL VKVAKEYGVK LTMFHGRGGT VGRGGGPTHL
    660 670 680 690 700
    AILSQPPDTI NGSLRVTVQG EVIEQSFGEE HLCFRTLQRF TAATLEHGMR
    710 720 730 740 750
    PPISPKPEWR ALLDEMAVVA TEEYRSVVFQ EPRFVEYFRL ATPELEYGRM
    760 770 780 790 800
    NIGSRPSKRK PSGGIESLRA IPWIFAWTQT RFHLPVWLGF GSAIRHVIEK
    810 820 830 840 850
    DVRNLHMLQD MYQHWPFFRV TIDLIEMVFA KGDPGIAALY DKLLVSEELW
    860 870 880 890 900
    PFGEKLRANF EETKKLILQT AGHKDLLEGD PYLKQRLRLR DSYITTLNVC
    910 920 930 940 950
    QAYTLKRIRD PSYHVTLRPH ISKEIAESSK PAKELIELNP TSEYAPGLED
    960
    TLILTMKGIA AGLQNTG
    Length:967
    Mass (Da):110,286
    Last modified:October 1, 2000 - v1
    Checksum:iBD3E8F98E2046EEE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ532901 mRNA Translation: CAD58725.1
    AC008007 Genomic DNA Translation: AAF69546.1
    CP002684 Genomic DNA Translation: AEE32921.1
    CP002684 Genomic DNA Translation: AEE32922.1
    CP002684 Genomic DNA Translation: AEE32923.1
    AY057507 mRNA Translation: AAL09748.1
    BT000647 mRNA Translation: AAN18213.1
    PIRiD96573
    RefSeqiNP_001031178.1, NM_001036101.2
    NP_001031179.1, NM_001036102.3
    NP_175738.1, NM_104209.3
    UniGeneiAt.23221

    Genome annotation databases

    EnsemblPlantsiAT1G53310.1; AT1G53310.1; AT1G53310
    AT1G53310.2; AT1G53310.2; AT1G53310
    AT1G53310.3; AT1G53310.3; AT1G53310
    GeneIDi841765
    GrameneiAT1G53310.1; AT1G53310.1; AT1G53310
    AT1G53310.2; AT1G53310.2; AT1G53310
    AT1G53310.3; AT1G53310.3; AT1G53310
    KEGGiath:AT1G53310

    Similar proteinsi

    Entry informationi

    Entry nameiCAPP1_ARATH
    AccessioniPrimary (citable) accession number: Q9MAH0
    Secondary accession number(s): Q546E4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 129 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health