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Q9MAH0

- CAPP1_ARATH

UniProt

Q9MAH0 - CAPP1_ARATH

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Protein

Phosphoenolpyruvate carboxylase 1

Gene

PPC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. Contributes probably to the adaptation to inorganic phosophate (Pi) deprivation.1 Publication

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

Cofactori

Mg2+1 Publication, Mn2+1 PublicationNote: Magnesium. Can also use manganese.1 Publication

Enzyme regulationi

By light-reversible phosphorylation (By similarity). Activated by inorganic phosophate (Pi) deprivation and glucose 6-phosphate. Inhibited by L-malate and L-aspartate.By similarity1 Publication

Kineticsi

  1. KM=0.18 mM for PEP (Phospho-PPC1 at pH 7.3 and 25 degrees Celsius)1 Publication
  2. KM=0.34 mM for PEP (Dephospho-PPC1 at pH 7.3 and 25 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 8-9.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei173 – 1731By similarity
Active sitei602 – 6021By similarity

GO - Molecular functioni

  1. phosphoenolpyruvate carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbon fixation Source: UniProtKB-KW
  2. cellular response to phosphate starvation Source: UniProtKB
  3. photosynthesis Source: UniProtKB-KW
  4. protein tetramerization Source: UniProtKB
  5. tricarboxylic acid cycle Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbon dioxide fixation, Photosynthesis

Keywords - Ligandi

Magnesium, Manganese

Enzyme and pathway databases

BioCyciARA:GQT-2761-MONOMER.
ARA:GQT-2762-MONOMER.
BRENDAi4.1.1.31. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylase 1 (EC:4.1.1.31)
Short name:
AtPPC1
Short name:
PEPC 1
Short name:
PEPCase 1
Alternative name(s):
107-kDa PEPC polypeptide
Gene namesi
Name:PPC1
Synonyms:p107
Ordered Locus Names:At1g53310
ORF Names:F12M16.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G53310.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. apoplast Source: TAIR
  2. cytosol Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Phosphoenolpyruvate carboxylase 1PRO_0000166657Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphoserine4 Publications
Modified residuei704 – 7041Phosphoserine1 Publication

Post-translational modificationi

Ubiquitinated.1 Publication
The phosphorylation of Ser-11 is reversibly promoted by inorganic phosophate (Pi) deprivation. Enhanced activity by phosphorylation at pH 7.3 by lowering Km and sensitivity to inhibition by L-malate and L-aspartate, while enhancing activation by glucose 6-phosphate.4 Publications

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9MAH0.
PRIDEiQ9MAH0.

Expressioni

Tissue specificityi

Expressed in all plant organs, with higher levels in roots.2 Publications

Inductioni

Upon inorganic phosophate (Pi) deprivation.1 Publication

Gene expression databases

ExpressionAtlasiQ9MAH0. baseline and differential.
GenevestigatoriQ9MAH0.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi26990. 4 interactions.
IntActiQ9MAH0. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9MAH0.
SMRiQ9MAH0. Positions 6-967.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 1 family.Curated

Phylogenomic databases

eggNOGiCOG2352.
HOGENOMiHOG000238648.
InParanoidiQ9MAH0.
KOiK01595.
OMAiEYETKHD.
PhylomeDBiQ9MAH0.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1.
InterProiIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 2 hits.
PROSITEiPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9MAH0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANRKLEKMA SIDVHLRQLV PGKVSEDDKL VEYDALLLDR FLDILQDLHG
60 70 80 90 100
EDLRETVQEL YEHSAEYEGK HEPKKLEELG SVLTSLDPGD SIVIAKAFSH
110 120 130 140 150
MLNLANLAEE VQIAYRRRIK KLKKGDFVDE SSATTESDLE ETFKKLVGDL
160 170 180 190 200
NKSPEEIFDA LKNQTVDLVL TAHPTQSVRR SLLQKHGRIR DCLAQLYAKD
210 220 230 240 250
ITPDDKQELD EALQREIQAA FRTDEIKRTP PTPQDEMRAG MSYFHETIWK
260 270 280 290 300
GVPKFLRRVD TALKNIGIEE RVPYNAPLIQ FSSWMGGDRD GNPRVTPEVT
310 320 330 340 350
RDVCLLARMM AATMYFNQIE DLMFEMSMWR CNDELRARAD EVHANSRKDA
360 370 380 390 400
AKHYIEFWKS IPTTEPYRVI LGDVRDKLYH TRERAHQLLS NGHSDVPVEA
410 420 430 440 450
TFINLEQFLE PLELCYRSLC SCGDRPIADG SLLDFLRQVS TFGLSLVRLD
460 470 480 490 500
IRQESDRHTD VLDAITTHLD IGSYREWSEE RRQEWLLSEL SGKRPLFGSD
510 520 530 540 550
LPKTEEIADV LDTFHVIAEL PADSFGAYII SMATAPSDVL AVELLQRECR
560 570 580 590 600
VKQPLRVVPL FEKLADLEAA PAAVARLFSV DWYKNRINGK QEVMIGYSDS
610 620 630 640 650
GKDAGRLSAA WQLYKAQEEL VKVAKEYGVK LTMFHGRGGT VGRGGGPTHL
660 670 680 690 700
AILSQPPDTI NGSLRVTVQG EVIEQSFGEE HLCFRTLQRF TAATLEHGMR
710 720 730 740 750
PPISPKPEWR ALLDEMAVVA TEEYRSVVFQ EPRFVEYFRL ATPELEYGRM
760 770 780 790 800
NIGSRPSKRK PSGGIESLRA IPWIFAWTQT RFHLPVWLGF GSAIRHVIEK
810 820 830 840 850
DVRNLHMLQD MYQHWPFFRV TIDLIEMVFA KGDPGIAALY DKLLVSEELW
860 870 880 890 900
PFGEKLRANF EETKKLILQT AGHKDLLEGD PYLKQRLRLR DSYITTLNVC
910 920 930 940 950
QAYTLKRIRD PSYHVTLRPH ISKEIAESSK PAKELIELNP TSEYAPGLED
960
TLILTMKGIA AGLQNTG
Length:967
Mass (Da):110,286
Last modified:October 1, 2000 - v1
Checksum:iBD3E8F98E2046EEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ532901 mRNA. Translation: CAD58725.1.
AC008007 Genomic DNA. Translation: AAF69546.1.
CP002684 Genomic DNA. Translation: AEE32921.1.
CP002684 Genomic DNA. Translation: AEE32922.1.
CP002684 Genomic DNA. Translation: AEE32923.1.
AY057507 mRNA. Translation: AAL09748.1.
BT000647 mRNA. Translation: AAN18213.1.
PIRiD96573.
RefSeqiNP_001031178.1. NM_001036101.1.
NP_001031179.1. NM_001036102.2.
NP_175738.1. NM_104209.2.
UniGeneiAt.23221.

Genome annotation databases

EnsemblPlantsiAT1G53310.1; AT1G53310.1; AT1G53310.
AT1G53310.2; AT1G53310.2; AT1G53310.
AT1G53310.3; AT1G53310.3; AT1G53310.
GeneIDi841765.
KEGGiath:AT1G53310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ532901 mRNA. Translation: CAD58725.1 .
AC008007 Genomic DNA. Translation: AAF69546.1 .
CP002684 Genomic DNA. Translation: AEE32921.1 .
CP002684 Genomic DNA. Translation: AEE32922.1 .
CP002684 Genomic DNA. Translation: AEE32923.1 .
AY057507 mRNA. Translation: AAL09748.1 .
BT000647 mRNA. Translation: AAN18213.1 .
PIRi D96573.
RefSeqi NP_001031178.1. NM_001036101.1.
NP_001031179.1. NM_001036102.2.
NP_175738.1. NM_104209.2.
UniGenei At.23221.

3D structure databases

ProteinModelPortali Q9MAH0.
SMRi Q9MAH0. Positions 6-967.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 26990. 4 interactions.
IntActi Q9MAH0. 2 interactions.

Proteomic databases

PaxDbi Q9MAH0.
PRIDEi Q9MAH0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G53310.1 ; AT1G53310.1 ; AT1G53310 .
AT1G53310.2 ; AT1G53310.2 ; AT1G53310 .
AT1G53310.3 ; AT1G53310.3 ; AT1G53310 .
GeneIDi 841765.
KEGGi ath:AT1G53310.

Organism-specific databases

GeneFarmi 5060. 479.
TAIRi AT1G53310.

Phylogenomic databases

eggNOGi COG2352.
HOGENOMi HOG000238648.
InParanoidi Q9MAH0.
KOi K01595.
OMAi EYETKHD.
PhylomeDBi Q9MAH0.

Enzyme and pathway databases

BioCyci ARA:GQT-2761-MONOMER.
ARA:GQT-2762-MONOMER.
BRENDAi 4.1.1.31. 399.

Gene expression databases

ExpressionAtlasi Q9MAH0. baseline and differential.
Genevestigatori Q9MAH0.

Family and domain databases

HAMAPi MF_00595. PEPcase_type1.
InterProi IPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view ]
Pfami PF00311. PEPcase. 1 hit.
[Graphical view ]
PRINTSi PR00150. PEPCARBXLASE.
SUPFAMi SSF51621. SSF51621. 2 hits.
PROSITEi PS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice."
    Sanchez R., Cejudo F.J.
    Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-704, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  6. "In vivo regulatory phosphorylation of the phosphoenolpyruvate carboxylase AtPPC1 in phosphate-starved Arabidopsis thaliana."
    Gregory A.L., Hurley B.A., Tran H.T., Valentine A.J., She Y.-M., Knowles V.L., Plaxton W.C.
    Biochem. J. 420:57-65(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-11, INDUCTION BY PHOSPHATE DEPRIVATION, ENZYME REGULATION, SUBUNIT, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR.
    Strain: cv. Columbia and cv. Landsberg erecta.
  7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCAPP1_ARATH
AccessioniPrimary (citable) accession number: Q9MAH0
Secondary accession number(s): Q546E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3