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Q9MAH0

- CAPP1_ARATH

UniProt

Q9MAH0 - CAPP1_ARATH

Protein

Phosphoenolpyruvate carboxylase 1

Gene

PPC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. Contributes probably to the adaptation to inorganic phosophate (Pi) deprivation.1 Publication

    Catalytic activityi

    Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

    Cofactori

    Magnesium. Can also use manganese.1 Publication

    Enzyme regulationi

    By light-reversible phosphorylation By similarity. Activated by inorganic phosophate (Pi) deprivation and glucose 6-phosphate. Inhibited by L-malate and L-aspartate.By similarity1 Publication

    Kineticsi

    1. KM=0.18 mM for PEP (Phospho-PPC1 at pH 7.3 and 25 degrees Celsius)1 Publication
    2. KM=0.34 mM for PEP (Dephospho-PPC1 at pH 7.3 and 25 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 8-9.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei173 – 1731By similarity
    Active sitei602 – 6021By similarity

    GO - Molecular functioni

    1. phosphoenolpyruvate carboxylase activity Source: UniProtKB-EC
    2. protein binding Source: TAIR

    GO - Biological processi

    1. carbon fixation Source: UniProtKB-KW
    2. cellular response to phosphate starvation Source: UniProtKB
    3. photosynthesis Source: UniProtKB-KW
    4. protein tetramerization Source: UniProtKB
    5. tricarboxylic acid cycle Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation, Photosynthesis

    Keywords - Ligandi

    Magnesium, Manganese

    Enzyme and pathway databases

    BioCyciARA:GQT-2761-MONOMER.
    ARA:GQT-2762-MONOMER.
    BRENDAi4.1.1.31. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylase 1 (EC:4.1.1.31)
    Short name:
    AtPPC1
    Short name:
    PEPC 1
    Short name:
    PEPCase 1
    Alternative name(s):
    107-kDa PEPC polypeptide
    Gene namesi
    Name:PPC1
    Synonyms:p107
    Ordered Locus Names:At1g53310
    ORF Names:F12M16.21
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G53310.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. apoplast Source: TAIR
    2. cytosol Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967Phosphoenolpyruvate carboxylase 1PRO_0000166657Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111Phosphoserine4 Publications
    Modified residuei704 – 7041Phosphoserine1 Publication

    Post-translational modificationi

    Ubiquitinated.1 Publication
    The phosphorylation of Ser-11 is reversibly promoted by inorganic phosophate (Pi) deprivation. Enhanced activity by phosphorylation at pH 7.3 by lowering Km and sensitivity to inhibition by L-malate and L-aspartate, while enhancing activation by glucose 6-phosphate.4 Publications

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiQ9MAH0.
    PRIDEiQ9MAH0.

    Expressioni

    Tissue specificityi

    Expressed in all plant organs, with higher levels in roots.2 Publications

    Inductioni

    Upon inorganic phosophate (Pi) deprivation.1 Publication

    Gene expression databases

    ArrayExpressiQ9MAH0.
    GenevestigatoriQ9MAH0.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi26990. 4 interactions.
    IntActiQ9MAH0. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9MAH0.
    SMRiQ9MAH0. Positions 6-967.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2352.
    HOGENOMiHOG000238648.
    InParanoidiQ9MAH0.
    KOiK01595.
    OMAiEYETKHD.
    PhylomeDBiQ9MAH0.

    Family and domain databases

    HAMAPiMF_00595. PEPcase_type1.
    InterProiIPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF00311. PEPcase. 1 hit.
    [Graphical view]
    PRINTSiPR00150. PEPCARBXLASE.
    SUPFAMiSSF51621. SSF51621. 2 hits.
    PROSITEiPS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9MAH0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANRKLEKMA SIDVHLRQLV PGKVSEDDKL VEYDALLLDR FLDILQDLHG    50
    EDLRETVQEL YEHSAEYEGK HEPKKLEELG SVLTSLDPGD SIVIAKAFSH 100
    MLNLANLAEE VQIAYRRRIK KLKKGDFVDE SSATTESDLE ETFKKLVGDL 150
    NKSPEEIFDA LKNQTVDLVL TAHPTQSVRR SLLQKHGRIR DCLAQLYAKD 200
    ITPDDKQELD EALQREIQAA FRTDEIKRTP PTPQDEMRAG MSYFHETIWK 250
    GVPKFLRRVD TALKNIGIEE RVPYNAPLIQ FSSWMGGDRD GNPRVTPEVT 300
    RDVCLLARMM AATMYFNQIE DLMFEMSMWR CNDELRARAD EVHANSRKDA 350
    AKHYIEFWKS IPTTEPYRVI LGDVRDKLYH TRERAHQLLS NGHSDVPVEA 400
    TFINLEQFLE PLELCYRSLC SCGDRPIADG SLLDFLRQVS TFGLSLVRLD 450
    IRQESDRHTD VLDAITTHLD IGSYREWSEE RRQEWLLSEL SGKRPLFGSD 500
    LPKTEEIADV LDTFHVIAEL PADSFGAYII SMATAPSDVL AVELLQRECR 550
    VKQPLRVVPL FEKLADLEAA PAAVARLFSV DWYKNRINGK QEVMIGYSDS 600
    GKDAGRLSAA WQLYKAQEEL VKVAKEYGVK LTMFHGRGGT VGRGGGPTHL 650
    AILSQPPDTI NGSLRVTVQG EVIEQSFGEE HLCFRTLQRF TAATLEHGMR 700
    PPISPKPEWR ALLDEMAVVA TEEYRSVVFQ EPRFVEYFRL ATPELEYGRM 750
    NIGSRPSKRK PSGGIESLRA IPWIFAWTQT RFHLPVWLGF GSAIRHVIEK 800
    DVRNLHMLQD MYQHWPFFRV TIDLIEMVFA KGDPGIAALY DKLLVSEELW 850
    PFGEKLRANF EETKKLILQT AGHKDLLEGD PYLKQRLRLR DSYITTLNVC 900
    QAYTLKRIRD PSYHVTLRPH ISKEIAESSK PAKELIELNP TSEYAPGLED 950
    TLILTMKGIA AGLQNTG 967
    Length:967
    Mass (Da):110,286
    Last modified:October 1, 2000 - v1
    Checksum:iBD3E8F98E2046EEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ532901 mRNA. Translation: CAD58725.1.
    AC008007 Genomic DNA. Translation: AAF69546.1.
    CP002684 Genomic DNA. Translation: AEE32921.1.
    CP002684 Genomic DNA. Translation: AEE32922.1.
    CP002684 Genomic DNA. Translation: AEE32923.1.
    AY057507 mRNA. Translation: AAL09748.1.
    BT000647 mRNA. Translation: AAN18213.1.
    PIRiD96573.
    RefSeqiNP_001031178.1. NM_001036101.1.
    NP_001031179.1. NM_001036102.2.
    NP_175738.1. NM_104209.2.
    UniGeneiAt.23221.

    Genome annotation databases

    EnsemblPlantsiAT1G53310.1; AT1G53310.1; AT1G53310.
    AT1G53310.2; AT1G53310.2; AT1G53310.
    AT1G53310.3; AT1G53310.3; AT1G53310.
    GeneIDi841765.
    KEGGiath:AT1G53310.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ532901 mRNA. Translation: CAD58725.1 .
    AC008007 Genomic DNA. Translation: AAF69546.1 .
    CP002684 Genomic DNA. Translation: AEE32921.1 .
    CP002684 Genomic DNA. Translation: AEE32922.1 .
    CP002684 Genomic DNA. Translation: AEE32923.1 .
    AY057507 mRNA. Translation: AAL09748.1 .
    BT000647 mRNA. Translation: AAN18213.1 .
    PIRi D96573.
    RefSeqi NP_001031178.1. NM_001036101.1.
    NP_001031179.1. NM_001036102.2.
    NP_175738.1. NM_104209.2.
    UniGenei At.23221.

    3D structure databases

    ProteinModelPortali Q9MAH0.
    SMRi Q9MAH0. Positions 6-967.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 26990. 4 interactions.
    IntActi Q9MAH0. 2 interactions.

    Proteomic databases

    PaxDbi Q9MAH0.
    PRIDEi Q9MAH0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G53310.1 ; AT1G53310.1 ; AT1G53310 .
    AT1G53310.2 ; AT1G53310.2 ; AT1G53310 .
    AT1G53310.3 ; AT1G53310.3 ; AT1G53310 .
    GeneIDi 841765.
    KEGGi ath:AT1G53310.

    Organism-specific databases

    GeneFarmi 5060. 479.
    TAIRi AT1G53310.

    Phylogenomic databases

    eggNOGi COG2352.
    HOGENOMi HOG000238648.
    InParanoidi Q9MAH0.
    KOi K01595.
    OMAi EYETKHD.
    PhylomeDBi Q9MAH0.

    Enzyme and pathway databases

    BioCyci ARA:GQT-2761-MONOMER.
    ARA:GQT-2762-MONOMER.
    BRENDAi 4.1.1.31. 399.

    Gene expression databases

    ArrayExpressi Q9MAH0.
    Genevestigatori Q9MAH0.

    Family and domain databases

    HAMAPi MF_00595. PEPcase_type1.
    InterProi IPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF00311. PEPcase. 1 hit.
    [Graphical view ]
    PRINTSi PR00150. PEPCARBXLASE.
    SUPFAMi SSF51621. SSF51621. 2 hits.
    PROSITEi PS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice."
      Sanchez R., Cejudo F.J.
      Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11 AND SER-704, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Root.
    6. "In vivo regulatory phosphorylation of the phosphoenolpyruvate carboxylase AtPPC1 in phosphate-starved Arabidopsis thaliana."
      Gregory A.L., Hurley B.A., Tran H.T., Valentine A.J., She Y.-M., Knowles V.L., Plaxton W.C.
      Biochem. J. 420:57-65(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT SER-11, INDUCTION BY PHOSPHATE DEPRIVATION, ENZYME REGULATION, SUBUNIT, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR.
      Strain: cv. Columbia and cv. Landsberg erecta.
    7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    8. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
    9. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCAPP1_ARATH
    AccessioniPrimary (citable) accession number: Q9MAH0
    Secondary accession number(s): Q546E4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3