Gibberellin receptor GID1A - Arabidopsis thaliana (Mouse-ear cress)

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Q9MAA7 (GID1A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 81. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Gibberellin receptor GID1A
EC=3.-.-.-

Alternative name(s):
AtCXE10
Carboxylesterase 10
GID1-like protein 1
Protein GA INSENSITIVE DWARF 1A
Short name=AtGID1A

Gene names

Name:	GID1A
Synonyms:	CXE10, GID1L1
Ordered Locus Names:	At3g05120
ORF Names:	T12H1.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination, stem elongation and flower development. Partially redundant with GID1B and GID1C. Ref.5 Ref.6 Ref.7
Subunit structure	Interacts (via N-terminus) with the DELLA proteins GAI, RGA, RGL1, RGL2 and RGL3 (via N-terminus) in a GA-dependent manner. Ref.5 Ref.6 Ref.8
Subcellular location	Nucleus By similarity.
Tissue specificity	Widely expressed. Ref.4
Disruption phenotype	No visible phenotype under normal growth condition. Ref.5 Ref.7
Sequence similarities	Belongs to the 'GDXG' lipolytic enzyme family.

Ontologies

Keywords
Biological process	Gibberellin signaling pathway
Cellular component	Nucleus
Molecular function	Hydrolase Receptor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	floral organ morphogenesis Inferred from genetic interaction Ref.5. Source: TAIR gibberellic acid mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW gibberellin mediated signaling pathway Inferred from genetic interaction Ref.7. Source: TAIR positive regulation of gibberellic acid mediated signaling pathway Inferred from genetic interaction Ref.5. Source: TAIR raffinose family oligosaccharide biosynthetic process Inferred from genetic interaction Ref.6. Source: TAIR
Cellular_component	cytoplasm Inferred from direct assay PubMed 17416730. Source: TAIR nucleus Inferred from direct assay PubMed 17416730. Source: TAIR
Molecular_function	gibberellin binding Inferred from direct assay Ref.9. Source: UniProtKB hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
GAI	Q9LQT8	6	EBI-963597,EBI-963606
GID2	Q9STX3	3	EBI-963597,EBI-619033
RGA	Q9SLH3	7	EBI-963597,EBI-963624
RGL2	Q8GXW1	3	EBI-963597,EBI-963665

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 345

345

Gibberellin receptor GID1A

PRO_0000071558

Regions

Region

115 – 116

Gibberellin binding

Sites

Active site

113

Potential

Active site

191

By similarity

Active site

289

By similarity

Binding site

127

Gibberellin

Binding site

191

Gibberellin

Binding site

238

Gibberellin; via carbonyl oxygen

Binding site

320

Gibberellin; via amide nitrogen

Secondary structure

345

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9MAA7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: FE1ED6E13BC1A48D
Show »

FASTA

345

38,618

        10         20         30         40         50         60 
MAASDEVNLI ESRTVVPLNT WVLISNFKVA YNILRRPDGT FNRHLAEYLD RKVTANANPV 

        70         80         90        100        110        120 
DGVFSFDVLI DRRINLLSRV YRPAYADQEQ PPSILDLEKP VDGDIVPVIL FFHGGSFAHS 

       130        140        150        160        170        180 
SANSAIYDTL CRRLVGLCKC VVVSVNYRRA PENPYPCAYD DGWIALNWVN SRSWLKSKKD 

       190        200        210        220        230        240 
SKVHIFLAGD SSGGNIAHNV ALRAGESGID VLGNILLNPM FGGNERTESE KSLDGKYFVT 

       250        260        270        280        290        300 
VRDRDWYWKA FLPEGEDREH PACNPFSPRG KSLEGVSFPK SLVVVAGLDL IRDWQLAYAE 

       310        320        330        340 
GLKKAGQEVK LMHLEKATVG FYLLPNNNHF HNVMDEISAF VNAEC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The carboxylesterase gene family from Arabidopsis thaliana." Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D. J. Mol. Evol. 57:487-500(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, GENE FAMILY.
[5]	"Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis." Griffiths J., Murase K., Rieu I., Zentella R., Zhang Z.L., Powers S.J., Gong F., Phillips A.L., Hedden P., Sun T.P., Thomas S.G. Plant Cell 18:3399-3414(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GAI AND RGA, DISRUPTION PHENOTYPE.
[6]	"Identification and characterization of Arabidopsis gibberellin receptors." Nakajima M., Shimada A., Takashi Y., Kim Y.C., Park S.H., Ueguchi-Tanaka M., Suzuki H., Katoh E., Iuchi S., Kobayashi M., Maeda T., Matsuoka M., Yamaguchi I. Plant J. 46:880-889(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GAI; RGA; RGL1; RGL2 AND RGL3.
[7]	"Multiple loss-of-function of Arabidopsis gibberellin receptor AtGID1s completely shuts down a gibberellin signal." Iuchi S., Suzuki H., Kim Y.C., Iuchi A., Kuromori T., Ueguchi-Tanaka M., Asami T., Yamaguchi I., Matsuoka M., Kobayashi M., Nakajima M. Plant J. 50:958-966(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"Differential expression and affinities of Arabidopsis gibberellin receptors can explain variation in phenotypes of multiple knock-out mutants." Suzuki H., Park S.-H., Okubo K., Kitamura J., Ueguchi-Tanaka M., Iuchi S., Katoh E., Kobayashi M., Yamaguchi I., Matsuoka M., Asami T., Nakajima M. Plant J. 60:48-55(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RGL2.
[9]	"Gibberellin-induced DELLA recognition by the gibberellin receptor GID1." Murase K., Hirano Y., Sun T.P., Hakoshima T. Nature 456:459-463(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 1-344 IN COMPLEX WITH GIBBERELLIN AND GAI.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AC009177 Genomic DNA. Translation: AAF27018.1.
CP002686 Genomic DNA. Translation: AEE74188.1.
AY136305 mRNA. Translation: AAM96971.1.
BT002605 mRNA. Translation: AAO00965.1.

IPI

IPI00527879.

RefSeq

NP_187163.1. NM_111384.3.

UniGene

At.18371.
At.74127.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ZSH	X-ray	1.80	A	1-344	[»]
2ZSI	X-ray	1.80	A	1-344	[»]

ProteinModelPortal

Q9MAA7.

SMR

Q9MAA7. Positions 6-344.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-37659N.

IntAct

Q9MAA7. 5 interactions.

STRING

3702.AT3G05120.1-P.

Proteomic databases

PaxDb

Q9MAA7.

PRIDE

Q9MAA7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G05120.1; AT3G05120.1; AT3G05120.

GeneID

819674.

KEGG

ath:AT3G05120.

Organism-specific databases

TAIR

At3g05120.

Phylogenomic databases

eggNOG

COG0657.

HOGENOM

HOG000152323.

InParanoid

Q9MAA7.

K14493.

OMA

FVLEANC.

PhylomeDB

Q9MAA7.

ProtClustDB

CLSN2684300.

Gene expression databases

Genevestigator

Q9MAA7.

GermOnline

AT3G05120. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR013094. AB_hydrolase_3.
IPR002168. Lipase_GDXG_AS.
[Graphical view]

Pfam

PF07859. Abhydrolase_3. 1 hit.
[Graphical view]

PROSITE

PS01173. LIPASE_GDXG_HIS. 1 hit.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9MAA7.

Entry information

Entry name

GID1A_ARATH

Accession

Primary (citable) accession number: Q9MAA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents