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Q9MAA7 (GID1A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gibberellin receptor GID1A

EC=3.-.-.-
Alternative name(s):
AtCXE10
Carboxylesterase 10
GID1-like protein 1
Protein GA INSENSITIVE DWARF 1A
Short name=AtGID1A
Gene names
Name:GID1A
Synonyms:CXE10, GID1L1
Ordered Locus Names:At3g05120
ORF Names:T12H1.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination, stem elongation and flower development. Partially redundant with GID1B and GID1C. Ref.5 Ref.6 Ref.7

Subunit structure

Interacts (via N-terminus) with the DELLA proteins GAI, RGA, RGL1, RGL2 and RGL3 (via N-terminus) in a GA-dependent manner. Ref.5 Ref.6 Ref.8

Subcellular location

Nucleus By similarity.

Tissue specificity

Widely expressed. Ref.4

Disruption phenotype

No visible phenotype under normal growth condition. Ref.5 Ref.7

Sequence similarities

Belongs to the 'GDXG' lipolytic enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 345344Gibberellin receptor GID1A
PRO_0000071558

Regions

Region115 – 1162Gibberellin binding
Motif113 – 1153Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole By similarity

Sites

Active site1131 Potential
Active site1911 By similarity
Active site2891 By similarity
Binding site1271Gibberellin
Binding site1911Gibberellin
Binding site2381Gibberellin; via carbonyl oxygen
Binding site3201Gibberellin; via amide nitrogen

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Secondary structure

...................................................... 345
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9MAA7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: FE1ED6E13BC1A48D

FASTA34538,618
        10         20         30         40         50         60 
MAASDEVNLI ESRTVVPLNT WVLISNFKVA YNILRRPDGT FNRHLAEYLD RKVTANANPV 

        70         80         90        100        110        120 
DGVFSFDVLI DRRINLLSRV YRPAYADQEQ PPSILDLEKP VDGDIVPVIL FFHGGSFAHS 

       130        140        150        160        170        180 
SANSAIYDTL CRRLVGLCKC VVVSVNYRRA PENPYPCAYD DGWIALNWVN SRSWLKSKKD 

       190        200        210        220        230        240 
SKVHIFLAGD SSGGNIAHNV ALRAGESGID VLGNILLNPM FGGNERTESE KSLDGKYFVT 

       250        260        270        280        290        300 
VRDRDWYWKA FLPEGEDREH PACNPFSPRG KSLEGVSFPK SLVVVAGLDL IRDWQLAYAE 

       310        320        330        340 
GLKKAGQEVK LMHLEKATVG FYLLPNNNHF HNVMDEISAF VNAEC 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"The carboxylesterase gene family from Arabidopsis thaliana."
Marshall S.D., Putterill J.J., Plummer K.M., Newcomb R.D.
J. Mol. Evol. 57:487-500(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY.
[5]"Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis."
Griffiths J., Murase K., Rieu I., Zentella R., Zhang Z.L., Powers S.J., Gong F., Phillips A.L., Hedden P., Sun T.P., Thomas S.G.
Plant Cell 18:3399-3414(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GAI AND RGA, DISRUPTION PHENOTYPE.
[6]"Identification and characterization of Arabidopsis gibberellin receptors."
Nakajima M., Shimada A., Takashi Y., Kim Y.C., Park S.H., Ueguchi-Tanaka M., Suzuki H., Katoh E., Iuchi S., Kobayashi M., Maeda T., Matsuoka M., Yamaguchi I.
Plant J. 46:880-889(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GAI; RGA; RGL1; RGL2 AND RGL3.
[7]"Multiple loss-of-function of Arabidopsis gibberellin receptor AtGID1s completely shuts down a gibberellin signal."
Iuchi S., Suzuki H., Kim Y.C., Iuchi A., Kuromori T., Ueguchi-Tanaka M., Asami T., Yamaguchi I., Matsuoka M., Kobayashi M., Nakajima M.
Plant J. 50:958-966(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]"Differential expression and affinities of Arabidopsis gibberellin receptors can explain variation in phenotypes of multiple knock-out mutants."
Suzuki H., Park S.-H., Okubo K., Kitamura J., Ueguchi-Tanaka M., Iuchi S., Katoh E., Kobayashi M., Yamaguchi I., Matsuoka M., Asami T., Nakajima M.
Plant J. 60:48-55(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RGL2.
[9]"Gibberellin-induced DELLA recognition by the gibberellin receptor GID1."
Murase K., Hirano Y., Sun T.P., Hakoshima T.
Nature 456:459-463(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 1-344 IN COMPLEX WITH GIBBERELLIN AND GAI.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC009177 Genomic DNA. Translation: AAF27018.1.
CP002686 Genomic DNA. Translation: AEE74188.1.
AY136305 mRNA. Translation: AAM96971.1.
BT002605 mRNA. Translation: AAO00965.1.
RefSeqNP_187163.1. NM_111384.3.
UniGeneAt.18371.
At.74127.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZSHX-ray1.80A1-344[»]
2ZSIX-ray1.80A1-344[»]
DisProtDP00723.
ProteinModelPortalQ9MAA7.
SMRQ9MAA7. Positions 6-344.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid5009. 4 interactions.
DIPDIP-37659N.
IntActQ9MAA7. 5 interactions.
STRING3702.AT3G05120.1-P.

Proteomic databases

PaxDbQ9MAA7.
PRIDEQ9MAA7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G05120.1; AT3G05120.1; AT3G05120.
GeneID819674.
KEGGath:AT3G05120.

Organism-specific databases

TAIRAT3G05120.

Phylogenomic databases

eggNOGCOG0657.
HOGENOMHOG000152323.
InParanoidQ9MAA7.
KOK14493.
OMAWERQDEL.
PhylomeDBQ9MAA7.

Enzyme and pathway databases

BioCycARA:AT3G05120-MONOMER.

Gene expression databases

GenevestigatorQ9MAA7.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
InterProIPR029058. AB_hydrolase.
IPR013094. AB_hydrolase_3.
IPR002168. Lipase_GDXG_AS.
[Graphical view]
PfamPF07859. Abhydrolase_3. 1 hit.
[Graphical view]
SUPFAMSSF53474. SSF53474. 1 hit.
PROSITEPS01173. LIPASE_GDXG_HIS. 1 hit.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9MAA7.

Entry information

Entry nameGID1A_ARATH
AccessionPrimary (citable) accession number: Q9MAA7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names