Reviewed, UniProtKB/Swiss-Prot Q9MA79 (F16P2_ARATH)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase, cytosolic
      Short name=FBPase
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase

Gene names

Ordered Locus Names:	At1g43670
ORF Names:	F2J6.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449204,EBI-449157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Fructose-1,6-bisphosphatase, cytosolic		PRO_0000200512
Regions
	Region	124 – 127	4	Substrate binding By similarity
Sites
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	100	1	Magnesium 1 By similarity
	Metal binding	100	1	Magnesium 2 By similarity
	Metal binding	121	1	Magnesium 2 By similarity
	Metal binding	121	1	Magnesium 3 By similarity
	Metal binding	123	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	124	1	Magnesium 3 By similarity
	Metal binding	283	1	Magnesium 3 By similarity
	Binding site	215	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity
	Binding site	267	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9MA79-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 00F18CD8588DADDA	FASTA	341	37,287
	10 20 30 40 50 60 MDHAADAHRT DLMTITRFVL NEQSKYPESR GDFTILLSHI VLGCKFVCSA VNKAGLAKLI 70 80 90 100 110 120 GLAGETNIQG EEQKKLDVLS NDVFVNALVS SGRTSVLVSE EDEEATFVEP SKRGKYCVVF 130 140 150 160 170 180 DPLDGSSNID CGVSIGTIFG IYTLDHTDEP TTADVLKPGN EMVAAGYCMY GSSCMLVLST 190 200 210 220 230 240 GTGVHGFTLD PSLGEFILTH PDIKIPNKGN IYSVNEGNAQ NWDGPTTKYV EKCKFPKDGS 250 260 270 280 290 300 PAKSLRYVGS MVADVHRTLL YGGIFLYPAD KKSPNGKLRV LYEVFPMSFL MEQAGGQAFT 310 320 330 340 GKKRALDLVP EKIHERSPIF LGSYDDVEEI KALYAEEEKK N

References

[1]

"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

[2]

"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC009526 Genomic DNA. Translation: AAF63117.1. BT000470 mRNA. Translation: AAN17447.1. BT008732 mRNA. Translation: AAP42745.1.
IPI	IPI00520560.
PIR	H96499.
RefSeq	NP_175032.1.
UniGene	At.12074 At.23691
3D structure databases
HSSP	HSSP built from PDB template 1BK4 based on UniProtKB P00637.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9MA79. 1 interaction.
Proteomic databases
PRIDE	Q9MA79.
Genome annotation databases
GeneID	840953.
GenomeReviews	Gene locus AT1G43670 in contig CT485782_GR.
KEGG	ath:AT1G43670.
NMPDR	fig|3702.1.peg.4049.
Organism-specific databases
TAIR	At1g43670.
Phylogenomic databases
OMA	Q9MA79. MADQAPF.
Enzyme and pathway databases
BRENDA	3.1.3.11. 302.
Gene expression databases
ArrayExpress	Q9MA79.
GermOnline	AT1G43670. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. IPR017955. IMPase/FBPase. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. FBPHPHTASE. PR00377. INFBPHPHTASE.
ProDom	PD001491. In_FB_phphtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P2_ARATH

Accession

Primary (citable) accession number: Q9MA79

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)