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Protein

Fructose-1,6-bisphosphatase, cytosolic

Gene

CYFBP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first irreversible reaction from fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate and plays an important regulatory role in sucrose biosynthesis and metabolism (Probable). Its activity is essential to regulate starch levels (PubMed:25743161). Functions in fructose-mediated signaling independently of its catalytic activity in sugar metabolism. May act downstream of ABA2/GIN1, which is involved in abscisic acid (ABA) synthesis to regulate autotrophic transition and modulate early seedling establishment after seed germination (PubMed:21253566).2 Publications2 Publications

Miscellaneous

In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.Curated

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi71Magnesium 1By similarity1
Metal bindingi100Magnesium 1By similarity1
Metal bindingi100Magnesium 2By similarity1
Metal bindingi121Magnesium 2By similarity1
Metal bindingi121Magnesium 3By similarity1
Metal bindingi123Magnesium 2; via carbonyl oxygenBy similarity1
Metal bindingi124Magnesium 3By similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei247SubstrateBy similarity1
Binding sitei267SubstrateBy similarity1
Binding sitei277SubstrateBy similarity1
Metal bindingi283Magnesium 3By similarity1

GO - Molecular functioni

  • fructose 1,6-bisphosphate 1-phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 1,6-bisphosphate metabolic process Source: TAIR
  • fructose 6-phosphate metabolic process Source: GO_Central
  • fructose metabolic process Source: GO_Central
  • gluconeogenesis Source: GO_Central
  • photosynthesis Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to fructose Source: TAIR
  • starch catabolic process Source: TAIR
  • sucrose biosynthetic process Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processCarbohydrate metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT1G43670-MONOMER.
MetaCyc:AT1G43670-MONOMER.
ReactomeiR-ATH-70263. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphatase, cytosolicCurated (EC:3.1.3.111 Publication)
Short name:
FBPaseCurated
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolaseCurated
Protein FRUCTOSE INSENSITIVE 11 Publication
Gene namesi
Name:CYFBP1 Publication
Synonyms:FINS11 Publication
Ordered Locus Names:At1g43670
ORF Names:F2J6.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G43670.
TAIRilocus:2031103. AT1G43670.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: TAIR

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Slight decreased in growth rate.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi126 – 127SS → AA: Loss of catalytic activity. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005121 – 341Fructose-1,6-bisphosphatase, cytosolicAdd BLAST341

Proteomic databases

PaxDbiQ9MA79.
PRIDEiQ9MA79.

Expressioni

Gene expression databases

ExpressionAtlasiQ9MA79. baseline and differential.
GenevisibleiQ9MA79. AT.

Interactioni

Protein-protein interaction databases

BioGridi26178. 1 interactor.
IntActiQ9MA79. 1 interactor.
STRINGi3702.AT1G43670.1.

Structurei

3D structure databases

ProteinModelPortaliQ9MA79.
SMRiQ9MA79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 127Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the FBPase class 1 family.Curated

Phylogenomic databases

eggNOGiKOG1458. Eukaryota.
COG0158. LUCA.
HOGENOMiHOG000191265.
InParanoidiQ9MA79.
KOiK03841.
OMAiQSGLVCR.
OrthoDBiEOG09360EJ7.
PhylomeDBiQ9MA79.

Family and domain databases

CDDicd00354. FBPase. 1 hit.
HAMAPiMF_01855. FBPase_class1. 1 hit.
InterProiView protein in InterPro
IPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiView protein in Pfam
PF00316. FBPase. 1 hit.
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiView protein in PROSITE
PS00124. FBPASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9MA79-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHAADAHRT DLMTITRFVL NEQSKYPESR GDFTILLSHI VLGCKFVCSA
60 70 80 90 100
VNKAGLAKLI GLAGETNIQG EEQKKLDVLS NDVFVNALVS SGRTSVLVSE
110 120 130 140 150
EDEEATFVEP SKRGKYCVVF DPLDGSSNID CGVSIGTIFG IYTLDHTDEP
160 170 180 190 200
TTADVLKPGN EMVAAGYCMY GSSCMLVLST GTGVHGFTLD PSLGEFILTH
210 220 230 240 250
PDIKIPNKGN IYSVNEGNAQ NWDGPTTKYV EKCKFPKDGS PAKSLRYVGS
260 270 280 290 300
MVADVHRTLL YGGIFLYPAD KKSPNGKLRV LYEVFPMSFL MEQAGGQAFT
310 320 330 340
GKKRALDLVP EKIHERSPIF LGSYDDVEEI KALYAEEEKK N
Length:341
Mass (Da):37,287
Last modified:October 1, 2000 - v1
Checksum:i00F18CD8588DADDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009526 Genomic DNA. Translation: AAF63117.1.
CP002684 Genomic DNA. Translation: AEE31985.1.
BT000470 mRNA. Translation: AAN17447.1.
BT008732 mRNA. Translation: AAP42745.1.
PIRiH96499.
RefSeqiNP_175032.1. NM_103492.5.
UniGeneiAt.12074.
At.23691.

Genome annotation databases

EnsemblPlantsiAT1G43670.1; AT1G43670.1; AT1G43670.
GeneIDi840953.
GrameneiAT1G43670.1; AT1G43670.1; AT1G43670.
KEGGiath:AT1G43670.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiF16P2_ARATH
AccessioniPrimary (citable) accession number: Q9MA79
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: October 1, 2000
Last modified: June 7, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families