Reviewed, UniProtKB/Swiss-Prot Q9M9Z2 (TPP2_ARATH)

Names and origin

Protein names

Recommended name:
    Probable thylakoidal processing peptidase 2, chloroplastic
    EC=3.4.21.89
Alternative name(s):
    Signal peptidase I-2

Gene names

Name:	TPP2
Ordered Locus Names:	At1g06870
ORF Names:	F4H5.6

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves the thylakoid-transfer domain from a chloroplast protein By similarity.
Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Plastid › chloroplast thylakoid membrane; Single-pass membrane protein. Note: located in the non-appressed lamellae of the thylakoid network By similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Thylakoid
Domain	Transit peptide Transmembrane
Molecular function	Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 68	68	Chloroplast Potential
	Transit peptide	69 – ?		Thylakoid Potential
	Chain	? – 367		Probable thylakoidal processing peptidase 2, chloroplastic		PRO_0000310735
Regions
	Transmembrane	185 – 205	21	Potential
	Topological domain	206 – 367	162	Lumenal, thylakoid Potential
Sites
	Active site	214	1	By similarity
Experimental info
	Sequence conflict	111	1	M → V in AAM62715. Ref.2
	Sequence conflict	196	1	V → F in AAM62715. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9M9Z2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: BE18813DE031BBAE	FASTA	367	40,150
	10 20 30 40 50 60 MAIRVTFTYS SYVARSIASS AGTRVGTGDV RSCFETWVRP RFCGHNQIPD IVDKSPGSNT 70 80 90 100 110 120 WGPSSGPRAR PASSMYSTIA REILEEGCKS PLVLGMISLM NLTGAPQFSG MTGLGISPFK 130 140 150 160 170 180 TSSVIPFLRG SKWMPCSIPA TLSTDIAEVD RGGKVCDPKV KLELSDKVSN GGNGWVNKLL 190 200 210 220 230 240 NICSEDAKAA FTAVTVSLLF RSALAEPKSI PSTSMLPTLD VGDRVIAEKV SYFFRKPEVS 250 260 270 280 290 300 DIVIFKAPPI LVEHGYSCAD VFIKRIVASE GDWVEVCDGK LLVNDTVQAE DFVLEPIDYE 310 320 330 340 350 360 MEPMFVPEGY VFVLGDNRNK SFDSHNWGPL PIKNIIGRSV FRYWPPSKVS DIIHHEQVSQ KRAVDVS

References

[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Arabidopsis ORF clones." Shinn P., Chen H., Kim C.J., Ecker J.R. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC011001 Genomic DNA. Translation: AAF63136.1. AY085489 mRNA. Translation: AAM62715.1. BT024745 mRNA. Translation: ABD59083.1.
IPI	IPI00517366.
PIR	E86203.
RefSeq	NP_172171.1.
UniGene	At.23784
3D structure databases
HSSP	HSSP built from PDB template 1KN9 based on UniProtKB P00803.
SMR	Q9M9Z2. Positions 201-348.
ModBase	Search...
Protein family/group databases
MEROPS	S26.A01.
Proteomic databases
PRIDE	Q9M9Z2.
Genome annotation databases
GeneID	837198.
GenomeReviews	Gene locus AT1G06870 in contig CT485782_GR.
KEGG	ath:AT1G06870.
NMPDR	fig|3702.1.peg.864.
Organism-specific databases
GeneFarm	2115. 205.
TAIR	At1g06870.
Phylogenomic databases
eggNOG	KOG0171.
HOGENOM	HBG595105.
InParanoid	Q9M9Z2.
OMA	PIKNIVG.
PhylomeDB	Q9M9Z2.
Enzyme and pathway databases
BRENDA	3.4.21.89. 302.
Gene expression databases
Genevestigator	Q9M9Z2.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPP2_ARATH

Accession

Primary (citable) accession number: Q9M9Z2
Secondary accession number(s): Q8LEC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)