Q9M9Y5 (GAUT6_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galacturonosyltransferase 6
EC=2.4.1.-

Gene names

Name:	GAUT6
Ordered Locus Names:	At1g06780
ORF Names:	F4H5.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably involved in pectin biosynthesis in cell walls. Ref.5
Pathway	Glycan metabolism; pectin biosynthesis.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Expressed in roots, inflorescences, siliques, leaves and stems. Ref.5
Disruption phenotype	Reduced galacturonic acid content in cell wall. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 8 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pectin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9M9Y5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Probable galacturonosyltransferase 6		PRO_0000392559
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	28 – 589	562	Lumenal Potential
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	317	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 02B583F503E4C205	FASTA	589	67,537
	10 20 30 40 50 60 MKQIRRWQRI LILALLSISV FAPLIFVSNR LKSITPVGRR EFIEELSKIR FTTNDLRLSA 70 80 90 100 110 120 IEHEDGEGLK GPRLILFKDG EFNSSAESDG GNTYKNREEQ VIVSQKMTVS SDEKGQILPT 130 140 150 160 170 180 VNQLANKTDF KPPLSKGEKN TRVQPDRATD VKTKEIRDKI IQAKAYLNFA PPGSNSQVVK 190 200 210 220 230 240 ELRGRLKELE RSVGDATKDK DLSKGALRRV KPMENVLYKA SRVFNNCPAI ATKLRAMNYN 250 260 270 280 290 300 TEEQVQAQKN QAAYLMQLAA RTTPKGLHCL SMRLTSEYFS LDPEKRQMPN QQNYFDANFN 310 320 330 340 350 360 HYVVFSDNVL ASSVVVNSTI SSSKEPERIV FHVVTDSLNY PAISMWFLLN IQSKATIQIL 370 380 390 400 410 420 NIDDMDVLPR DYDQLLMKQN SNDPRFISTL NHARFYLPDI FPGLNKMVLL DHDVVVQRDL 430 440 450 460 470 480 SRLWSIDMKG KVVGAVETCL EGESSFRSMS TFINFSDTWV AGKFSPRACT WAFGMNLIDL 490 500 510 520 530 540 EEWRIRKLTS TYIKYFNLGT KRPLWKAGSL PIGWLTFYRQ TLALDKRWHV MGLGRESGVK 550 560 570 580 AVDIEQAAVI HYDGVMKPWL DIGKENYKRY WNIHVPYHHT YLQQCNLQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase." Sterling J.D., Atmodjo M.A., Inwood S.E., Kumar Kolli V.S., Quigley H.F., Hahn M.G., Mohnen D. Proc. Natl. Acad. Sci. U.S.A. 103:5236-5241(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"Arabidopsis thaliana T-DNA mutants implicate GAUT genes in the biosynthesis of pectin and xylan in cell walls and seed testa." Caffall K.H., Pattathil S., Phillips S.E., Hahn M.G., Mohnen D. Mol. Plant 2:1000-1014(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC011001 Genomic DNA. Translation: AAF63140.1. CP002684 Genomic DNA. Translation: AEE28035.1. AY045900 mRNA. Translation: AAK76574.1. AY091452 mRNA. Translation: AAM14391.1.
IPI	IPI00528239.
PIR	F86202.
RefSeq	NP_563771.1. NM_100555.2.
UniGene	At.12086.
3D structure databases
ProteinModelPortal	Q9M9Y5.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G06780.1-P.
Protein family/group databases
CAZy	GT8. Glycosyltransferase Family 8.
Proteomic databases
PaxDb	Q9M9Y5.
PRIDE	Q9M9Y5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G06780.1; AT1G06780.1; AT1G06780.
GeneID	837189.
KEGG	ath:AT1G06780.
Organism-specific databases
TAIR	At1g06780.
Phylogenomic databases
eggNOG	NOG260334.
HOGENOM	HOG000239479.
InParanoid	Q9M9Y5.
KO	K13648.
PhylomeDB	Q9M9Y5.
ProtClustDB	PLN02718.
Enzyme and pathway databases
UniPathway	UPA00845.
Gene expression databases
ArrayExpress	Q9M9Y5.
Genevestigator	Q9M9Y5.
Family and domain databases
InterPro	IPR002495. Glyco_trans_8. [Graphical view]
Pfam	PF01501. Glyco_transf_8. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAUT6_ARATH

Accession

Primary (citable) accession number: Q9M9Y5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program