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Protein

Phospholipase D zeta 2

Gene

PLPZETA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Phosphatidylcholine-selective (PubMed:11891260). Regulates vesicle trafficking and auxin responses (PubMed:17259265). Required for the normal cycling of PIN-2 containing vesicles (PubMed:17259265). Contributes to the supply of inorganic phosphorus for cell metabolism and diacylglycerol moieties for galactolipid synthesis in phosphorus-starved roots (PubMed:16891548, PubMed:16617110). Involved in root elongation during phosphate limitation (PubMed:16384909).5 Publications

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.1 Publication

Cofactori

Note: Does not require Ca2+ or any other cation for activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei477PROSITE-ProRule annotation1
Active sitei479PROSITE-ProRule annotation1
Active sitei484PROSITE-ProRule annotation1
Active sitei852PROSITE-ProRule annotation1
Active sitei854PROSITE-ProRule annotation1
Active sitei859PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cellular response to nitrogen starvation Source: TAIR
  • cellular response to phosphate starvation Source: TAIR
  • galactolipid biosynthetic process Source: TAIR
  • inositol lipid-mediated signaling Source: InterPro
  • lipid biosynthetic process Source: TAIR
  • phosphatidic acid biosynthetic process Source: InterPro
  • phospholipid catabolic process Source: TAIR
  • regulation of vesicle-mediated transport Source: TAIR
  • response to auxin Source: TAIR
  • root development Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G05630-MONOMER
BRENDAi3.1.4.4 399
ReactomeiR-ATH-1483166 Synthesis of PA
R-ATH-2029485 Role of phospholipids in phagocytosis
R-ATH-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D zeta 21 Publication (EC:3.1.4.41 Publication)
Short name:
PLDzeta21 Publication
Alternative name(s):
Phospholipase D p2
Short name:
AtPLDp2
Phospholipase D2 PHOX and PX-containing domain protein
Gene namesi
Name:PLPZETA21 Publication
Synonyms:PLDP2
Ordered Locus Names:At3g05630Imported
ORF Names:F18C1.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G05630
TAIRilocus:2078037 AT3G05630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions (PubMed:16384909). Shorter roots less sensitive to exogenous auxin (PubMed:17259265). Reduced gravitropism (PubMed:17259265). No effect on root hair patterning or root hair growth (PubMed:16384909). Defective in the hydrolysis of phospholipids, reduced capacity to accumulate galactolipids and premature change in root architecture in response to phosphate starvation (PubMed:16617110). Decreased accumulation of phosphatidic acid in roots under phosphate-limited conditions (PubMed:16384909). No effect on the concentration of phospholipids and galactolipids in phosphorus-starved roots (PubMed:16891548). Pldzeta1 and pldzeta2 double mutants show a smaller decrease in phosphatidylcholine and a smaller increase in digalactosyldiacylglycerol in phosphorus-starved roots (PubMed:16891548). Pldzeta1 and pldzeta2 double mutants show reduced primary root elongation and increased lateral root elongation under low-phosphate conditions (PubMed:16384909).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002188191 – 1046Phospholipase D zeta 2Add BLAST1046

Proteomic databases

PaxDbiQ9M9W8

Expressioni

Tissue specificityi

Expressed in seedlings, roots, leaves, stems and flowers (PubMed:17259265, PubMed:16384909). Highest expression in roots (PubMed:16384909). Detected only in the meristematic regions up to 4 days after germination and then at later stages in all tissues (PubMed:16617110).3 Publications

Inductioni

Up-regulated by auxin (PubMed:17259265). Up-regulated by phosphate limitation (PubMed:16384909, PubMed:16617110).3 Publications

Gene expression databases

ExpressionAtlasiQ9M9W8 differential
GenevisibleiQ9M9W8 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G05630.1

Structurei

3D structure databases

ProteinModelPortaliQ9M9W8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 205PXAdd BLAST161
Domaini215 – 343PHPROSITE-ProRule annotationAdd BLAST129
Domaini472 – 499PLD phosphodiesterase 1PROSITE-ProRule annotationAdd BLAST28
Domaini847 – 874PLD phosphodiesterase 2PROSITE-ProRule annotationAdd BLAST28

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329 Eukaryota
COG1502 LUCA
HOGENOMiHOG000246972
InParanoidiQ9M9W8
KOiK01115
OMAiMEFQYNS
OrthoDBiEOG093600S3
PhylomeDBiQ9M9W8

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR025202 PLD-like_dom
IPR001736 PLipase_D/transphosphatidylase
IPR016555 PLipase_D_euk
IPR015679 PLipase_D_fam
PANTHERiPTHR18896 PTHR18896, 2 hits
PfamiView protein in Pfam
PF00614 PLDc, 1 hit
PF13091 PLDc_2, 1 hit
PIRSFiPIRSF009376 Phospholipase_D_euk, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00155 PLDc, 2 hits
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50035 PLD, 2 hits

Sequencei

Sequence statusi: Complete.

Q9M9W8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDKLLLPN GVKSDGVIRM TRADAAAAAA SSSLGGGSQI FDELPKAAIV
60 70 80 90 100
SVSRPDTTDF SPLLLSYTLE LQYKQFKWTL QKKASQVLYL HFALKKRLII
110 120 130 140 150
EELHDKQEQV REWLHSLGIF DMQGSVVQDD EEPDDGALPL HYTEDSIKNR
160 170 180 190 200
NVPSRAALPI IRPTIGRSET VVDRGRTAMQ GYLSLFLGNL DIVNSKEVCK
210 220 230 240 250
FLEVSRLSFA REYGSKMKEG YVTVKHLRDV PGSDGVRCCL PTHCLGFFGT
260 270 280 290 300
SWTKVWAVLK PGFLALLEDP FSGKLLDIMV FDTLGLQGTK ESSEQPRLAE
310 320 330 340 350
QVKEHNPLRF GFKVTSGDRT VRLRTTSSRK VKEWVKAVDE AGCYSPHRFG
360 370 380 390 400
SFAPPRGLTS DGSQAQWFVD GHTAFEAIAF AIQNATSEIF MTGWWLCPEL
410 420 430 440 450
YLKRPFEDHP SLRLDALLET KAKQGVKIYI LLYKEVQIAL KINSLYSKKR
460 470 480 490 500
LQNIHKNVKV LRYPDHLSSG IYLWSHHEKI VIVDYQVCFI GGLDLCFGRY
510 520 530 540 550
DTAEHKIGDC PPYIWPGKDY YNPRESEPNS WEETMKDELD RRKYPRMPWH
560 570 580 590 600
DVHCALWGPP CRDVARHFVQ RWNHSKRNKA PNEQTIPLLM PHHHMVLPHY
610 620 630 640 650
LGTREIDIIA AAKPEEDPDK PVVLARHDSF SSASPPQEIP LLLPQETDAD
660 670 680 690 700
FAGRGDLKLD SGARQDPGET SEESDLDEAV NDWWWQIGKQ SDCRCQIIRS
710 720 730 740 750
VSQWSAGTSQ PEDSIHRAYC SLIQNAEHFI YIENQFFISG LEKEDTILNR
760 770 780 790 800
VLEALYRRIL KAHEENKCFR VVIVIPLLPG FQGGIDDFGA ATVRALMHWQ
810 820 830 840 850
YRTISREGTS ILDNLNALLG PKTQDYISFY GLRSYGRLFE DGPIATSQIY
860 870 880 890 900
VHSKLMIVDD RIAVIGSSNI NDRSLLGSRD SEIGVVIEDK EFVESSMNGM
910 920 930 940 950
KWMAGKFSYS LRCSLWSEHL GLHAGEIQKI EDPIKDATYK DLWMATAKKN
960 970 980 990 1000
TDIYNQVFSC IPNEHIRSRA ALRHNMALCK DKLGHTTIDL GIAPERLESC
1010 1020 1030 1040
GSDSWEILKE TRGNLVCFPL QFMCDQEDLR PGFNESEFYT APQVFH
Length:1,046
Mass (Da):118,810
Last modified:December 20, 2005 - v2
Checksum:iDEF5E388891E5D23
GO

Sequence cautioni

The sequence AAF26134 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY305003 mRNA Translation: AAP68834.1
AM182458 mRNA Translation: CAJ58441.1
AC011620 Genomic DNA Translation: AAF26134.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE74269.1
RefSeqiNP_187214.2, NM_111436.6
UniGeneiAt.40640

Genome annotation databases

EnsemblPlantsiAT3G05630.1; AT3G05630.1; AT3G05630
GeneIDi819730
GrameneiAT3G05630.1; AT3G05630.1; AT3G05630
KEGGiath:AT3G05630

Similar proteinsi

Entry informationi

Entry nameiPLDZ2_ARATH
AccessioniPrimary (citable) accession number: Q9M9W8
Secondary accession number(s): A3KH17, Q7XZQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: December 20, 2005
Last modified: May 23, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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