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Protein

Phospholipase D zeta 2

Gene

PLPZETA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Phosphatidylcholine-selective (PubMed:11891260). Regulates vesicle trafficking and auxin responses (PubMed:17259265). Required for the normal cycling of PIN-2 containing vesicles (PubMed:17259265). Contributes to the supply of inorganic phosphorus for cell metabolism and diacylglycerol moieties for galactolipid synthesis in phosphorus-starved roots (PubMed:16891548, PubMed:16617110). Involved in root elongation during phosphate limitation (PubMed:16384909).5 Publications

Catalytic activityi

A phosphatidylcholine + H2O = choline + a phosphatidate.1 Publication

Cofactori

Note: Does not require Ca2+ or any other cation for activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei477 – 4771PROSITE-ProRule annotation
Active sitei479 – 4791PROSITE-ProRule annotation
Active sitei484 – 4841PROSITE-ProRule annotation
Active sitei852 – 8521PROSITE-ProRule annotation
Active sitei854 – 8541PROSITE-ProRule annotation
Active sitei859 – 8591PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to nitrogen starvation Source: TAIR
  • cellular response to phosphate starvation Source: TAIR
  • galactolipid biosynthetic process Source: TAIR
  • phospholipid catabolic process Source: TAIR
  • regulation of vesicle-mediated transport Source: TAIR
  • response to auxin Source: TAIR
  • root development Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT3G05630-MONOMER.
BRENDAi3.1.4.4. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-2029485. Role of phospholipids in phagocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase D zeta 21 Publication (EC:3.1.4.41 Publication)
Short name:
PLDzeta21 Publication
Alternative name(s):
Phospholipase D p2
Short name:
AtPLDp2
Phospholipase D2 PHOX and PX-containing domain protein
Gene namesi
Name:PLPZETA21 Publication
Synonyms:PLDP2
Ordered Locus Names:At3g05630Imported
ORF Names:F18C1.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G05630.

Subcellular locationi

GO - Cellular componenti

  • vacuole Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype when grown under normal conditions (PubMed:16384909). Shorter roots less sensitive to exogenous auxin (PubMed:17259265). Reduced gravitropism (PubMed:17259265). No effect on root hair patterning or root hair growth (PubMed:16384909). Defective in the hydrolysis of phospholipids, reduced capacity to accumulate galactolipids and premature change in root architecture in response to phosphate starvation (PubMed:16617110). Decreased accumulation of phosphatidic acid in roots under phosphate-limited conditions (PubMed:16384909). No effect on the concentration of phospholipids and galactolipids in phosphorus-starved roots (PubMed:16891548). Pldzeta1 and pldzeta2 double mutants show a smaller decrease in phosphatidylcholine and a smaller increase in digalactosyldiacylglycerol in phosphorus-starved roots (PubMed:16891548). Pldzeta1 and pldzeta2 double mutants show reduced primary root elongation and increased lateral root elongation under low-phosphate conditions (PubMed:16384909).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10461045Phospholipase D zeta 2PRO_0000218819Add
BLAST

Proteomic databases

PaxDbiQ9M9W8.
PRIDEiQ9M9W8.

Expressioni

Tissue specificityi

Expressed in seedlings, roots, leaves, stems and flowers (PubMed:17259265, PubMed:16384909). Highest expression in roots (PubMed:16384909). Detected only in the meristematic regions up to 4 days after germination and then at later stages in all tissues (PubMed:16617110).3 Publications

Inductioni

Up-regulated by auxin (PubMed:17259265). Up-regulated by phosphate limitation (PubMed:16384909, PubMed:16617110).3 Publications

Gene expression databases

ExpressionAtlasiQ9M9W8. baseline and differential.
GenevisibleiQ9M9W8. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G05630.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M9W8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 205161PXAdd
BLAST
Domaini215 – 343129PHPROSITE-ProRule annotationAdd
BLAST
Domaini472 – 49928PLD phosphodiesterase 1PROSITE-ProRule annotationAdd
BLAST
Domaini847 – 87428PLD phosphodiesterase 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 2 PLD phosphodiesterase domains.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
HOGENOMiHOG000246972.
InParanoidiQ9M9W8.
KOiK01115.
OMAiFSYSLRC.
OrthoDBiEOG093600S3.
PhylomeDBiQ9M9W8.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 2 hits.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50035. PLD. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M9W8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDKLLLPN GVKSDGVIRM TRADAAAAAA SSSLGGGSQI FDELPKAAIV
60 70 80 90 100
SVSRPDTTDF SPLLLSYTLE LQYKQFKWTL QKKASQVLYL HFALKKRLII
110 120 130 140 150
EELHDKQEQV REWLHSLGIF DMQGSVVQDD EEPDDGALPL HYTEDSIKNR
160 170 180 190 200
NVPSRAALPI IRPTIGRSET VVDRGRTAMQ GYLSLFLGNL DIVNSKEVCK
210 220 230 240 250
FLEVSRLSFA REYGSKMKEG YVTVKHLRDV PGSDGVRCCL PTHCLGFFGT
260 270 280 290 300
SWTKVWAVLK PGFLALLEDP FSGKLLDIMV FDTLGLQGTK ESSEQPRLAE
310 320 330 340 350
QVKEHNPLRF GFKVTSGDRT VRLRTTSSRK VKEWVKAVDE AGCYSPHRFG
360 370 380 390 400
SFAPPRGLTS DGSQAQWFVD GHTAFEAIAF AIQNATSEIF MTGWWLCPEL
410 420 430 440 450
YLKRPFEDHP SLRLDALLET KAKQGVKIYI LLYKEVQIAL KINSLYSKKR
460 470 480 490 500
LQNIHKNVKV LRYPDHLSSG IYLWSHHEKI VIVDYQVCFI GGLDLCFGRY
510 520 530 540 550
DTAEHKIGDC PPYIWPGKDY YNPRESEPNS WEETMKDELD RRKYPRMPWH
560 570 580 590 600
DVHCALWGPP CRDVARHFVQ RWNHSKRNKA PNEQTIPLLM PHHHMVLPHY
610 620 630 640 650
LGTREIDIIA AAKPEEDPDK PVVLARHDSF SSASPPQEIP LLLPQETDAD
660 670 680 690 700
FAGRGDLKLD SGARQDPGET SEESDLDEAV NDWWWQIGKQ SDCRCQIIRS
710 720 730 740 750
VSQWSAGTSQ PEDSIHRAYC SLIQNAEHFI YIENQFFISG LEKEDTILNR
760 770 780 790 800
VLEALYRRIL KAHEENKCFR VVIVIPLLPG FQGGIDDFGA ATVRALMHWQ
810 820 830 840 850
YRTISREGTS ILDNLNALLG PKTQDYISFY GLRSYGRLFE DGPIATSQIY
860 870 880 890 900
VHSKLMIVDD RIAVIGSSNI NDRSLLGSRD SEIGVVIEDK EFVESSMNGM
910 920 930 940 950
KWMAGKFSYS LRCSLWSEHL GLHAGEIQKI EDPIKDATYK DLWMATAKKN
960 970 980 990 1000
TDIYNQVFSC IPNEHIRSRA ALRHNMALCK DKLGHTTIDL GIAPERLESC
1010 1020 1030 1040
GSDSWEILKE TRGNLVCFPL QFMCDQEDLR PGFNESEFYT APQVFH
Length:1,046
Mass (Da):118,810
Last modified:December 20, 2005 - v2
Checksum:iDEF5E388891E5D23
GO

Sequence cautioni

The sequence AAF26134 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY305003 mRNA. Translation: AAP68834.1.
AM182458 mRNA. Translation: CAJ58441.1.
AC011620 Genomic DNA. Translation: AAF26134.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74269.1.
RefSeqiNP_187214.2. NM_111436.5.
UniGeneiAt.40640.

Genome annotation databases

EnsemblPlantsiAT3G05630.1; AT3G05630.1; AT3G05630.
GeneIDi819730.
GrameneiAT3G05630.1; AT3G05630.1; AT3G05630.
KEGGiath:AT3G05630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY305003 mRNA. Translation: AAP68834.1.
AM182458 mRNA. Translation: CAJ58441.1.
AC011620 Genomic DNA. Translation: AAF26134.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74269.1.
RefSeqiNP_187214.2. NM_111436.5.
UniGeneiAt.40640.

3D structure databases

ProteinModelPortaliQ9M9W8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G05630.1.

Proteomic databases

PaxDbiQ9M9W8.
PRIDEiQ9M9W8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G05630.1; AT3G05630.1; AT3G05630.
GeneIDi819730.
GrameneiAT3G05630.1; AT3G05630.1; AT3G05630.
KEGGiath:AT3G05630.

Organism-specific databases

TAIRiAT3G05630.

Phylogenomic databases

eggNOGiKOG1329. Eukaryota.
COG1502. LUCA.
HOGENOMiHOG000246972.
InParanoidiQ9M9W8.
KOiK01115.
OMAiFSYSLRC.
OrthoDBiEOG093600S3.
PhylomeDBiQ9M9W8.

Enzyme and pathway databases

BioCyciARA:AT3G05630-MONOMER.
BRENDAi3.1.4.4. 399.
ReactomeiR-ATH-1483166. Synthesis of PA.
R-ATH-2029485. Role of phospholipids in phagocytosis.

Miscellaneous databases

PROiQ9M9W8.

Gene expression databases

ExpressionAtlasiQ9M9W8. baseline and differential.
GenevisibleiQ9M9W8. AT.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR025202. PLD-like_dom.
IPR001736. PLipase_D/transphosphatidylase.
IPR015679. PLipase_D_fam.
[Graphical view]
PANTHERiPTHR18896. PTHR18896. 2 hits.
PfamiPF00614. PLDc. 1 hit.
PF13091. PLDc_2. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00155. PLDc. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50035. PLD. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLDZ2_ARATH
AccessioniPrimary (citable) accession number: Q9M9W8
Secondary accession number(s): A3KH17, Q7XZQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.