Reviewed, UniProtKB/Swiss-Prot Q9M9W8 (PLDP2_ARATH)

Names and origin

Protein names

Recommended name:
    Phospholipase D p2
      Short name=AtPLDp2
    EC=3.1.4.4
Alternative name(s):
    Phospholipase D2 PHOX and PX-containing domain protein
    Phospholipase D zeta 2
      Short name=PLDzeta2

Gene names

Name:	PLDP2
Ordered Locus Names:	At3g05630
ORF Names:	F18C1.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1046 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Phosphatidylcholine-selective.
Catalytic activity	A phosphatidylcholine + H2O = choline + a phosphatidate.
Enzyme regulation	Calcium-independent and PIP2-dependent.
Sequence similarities	Belongs to the phospholipase D family. PXPH-PLD subfamily. Contains 1 PH domain. Contains 2 PLD phosphodiesterase domains. Contains 1 PX (phox homology) domain.
Sequence caution	The sequence AAF26134.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lipid degradation
Domain	Repeat
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular response to nitrogen starvation Inferred from expression pattern. Source: TAIR cellular response to phosphate starvation Inferred from expression pattern. Source: TAIR galactolipid biosynthetic process Inferred from mutant phenotype. Source: TAIR phospholipid catabolic process Inferred from mutant phenotype. Source: TAIR response to auxin stimulus Inferred from mutant phenotype. Source: TAIR root development Inferred from genetic interaction. Source: TAIR
Cellular component	vacuole Inferred from direct assay. Source: TAIR
Molecular function	NAPE-specific phospholipase D activity Inferred from electronic annotation. Source: EC phospholipase D activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1046	1046	Phospholipase D p2		PRO_0000218819
Regions
	Domain	45 – 205	161	PX
	Domain	215 – 343	129	PH
	Domain	472 – 499	28	PLD phosphodiesterase 1
	Domain	847 – 874	28	PLD phosphodiesterase 2
Sites
	Active site	477	1	Potential
	Active site	479	1	Potential
	Active site	484	1	Potential
	Active site	852	1	Potential
	Active site	854	1	Potential
	Active site	859	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9M9W8-1 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: DEF5E388891E5D23	FASTA	1,046	118,810
	10 20 30 40 50 60 MSTDKLLLPN GVKSDGVIRM TRADAAAAAA SSSLGGGSQI FDELPKAAIV SVSRPDTTDF 70 80 90 100 110 120 SPLLLSYTLE LQYKQFKWTL QKKASQVLYL HFALKKRLII EELHDKQEQV REWLHSLGIF 130 140 150 160 170 180 DMQGSVVQDD EEPDDGALPL HYTEDSIKNR NVPSRAALPI IRPTIGRSET VVDRGRTAMQ 190 200 210 220 230 240 GYLSLFLGNL DIVNSKEVCK FLEVSRLSFA REYGSKMKEG YVTVKHLRDV PGSDGVRCCL 250 260 270 280 290 300 PTHCLGFFGT SWTKVWAVLK PGFLALLEDP FSGKLLDIMV FDTLGLQGTK ESSEQPRLAE 310 320 330 340 350 360 QVKEHNPLRF GFKVTSGDRT VRLRTTSSRK VKEWVKAVDE AGCYSPHRFG SFAPPRGLTS 370 380 390 400 410 420 DGSQAQWFVD GHTAFEAIAF AIQNATSEIF MTGWWLCPEL YLKRPFEDHP SLRLDALLET 430 440 450 460 470 480 KAKQGVKIYI LLYKEVQIAL KINSLYSKKR LQNIHKNVKV LRYPDHLSSG IYLWSHHEKI 490 500 510 520 530 540 VIVDYQVCFI GGLDLCFGRY DTAEHKIGDC PPYIWPGKDY YNPRESEPNS WEETMKDELD 550 560 570 580 590 600 RRKYPRMPWH DVHCALWGPP CRDVARHFVQ RWNHSKRNKA PNEQTIPLLM PHHHMVLPHY 610 620 630 640 650 660 LGTREIDIIA AAKPEEDPDK PVVLARHDSF SSASPPQEIP LLLPQETDAD FAGRGDLKLD 670 680 690 700 710 720 SGARQDPGET SEESDLDEAV NDWWWQIGKQ SDCRCQIIRS VSQWSAGTSQ PEDSIHRAYC 730 740 750 760 770 780 SLIQNAEHFI YIENQFFISG LEKEDTILNR VLEALYRRIL KAHEENKCFR VVIVIPLLPG 790 800 810 820 830 840 FQGGIDDFGA ATVRALMHWQ YRTISREGTS ILDNLNALLG PKTQDYISFY GLRSYGRLFE 850 860 870 880 890 900 DGPIATSQIY VHSKLMIVDD RIAVIGSSNI NDRSLLGSRD SEIGVVIEDK EFVESSMNGM 910 920 930 940 950 960 KWMAGKFSYS LRCSLWSEHL GLHAGEIQKI EDPIKDATYK DLWMATAKKN TDIYNQVFSC 970 980 990 1000 1010 1020 IPNEHIRSRA ALRHNMALCK DKLGHTTIDL GIAPERLESC GSDSWEILKE TRGNLVCFPL 1030 1040 QFMCDQEDLR PGFNESEFYT APQVFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of Arabidopsis phospholipase D zeta2." Qin C., Wang X. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY305003 mRNA. Translation: AAP68834.1. AC011620 Genomic DNA. Translation: AAF26134.1. Sequence problems.
IPI	IPI00530986.
RefSeq	NP_187214.2.
UniGene	At.40640
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	819730.
GenomeReviews	Gene locus AT3G05630 in contig BA000014_GR.
KEGG	ath:AT3G05630.
NMPDR	fig|3702.1.peg.12549.
Organism-specific databases
TAIR	At3g05630.
Phylogenomic databases
OMA	Q9M9W8. NEQTIPL.
Enzyme and pathway databases
BRENDA	3.1.4.4. 302.
Gene expression databases
ArrayExpress	Q9M9W8.
GermOnline	AT3G05630. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR015679. Phospholipase_D. IPR001849. Pleckstrin_homology. IPR001736. PLipase_D/transphosphatidylase. IPR016555. PLipase_D_euk. [Graphical view]
PANTHER	PTHR18896. Phospholipase_D. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00614. PLDc. 2 hits. [Graphical view]
PIRSF	PIRSF009376. Phospholipase_D_euk. 1 hit.
SMART	SM00233. PH. 1 hit. SM00155. PLDc. 2 hits. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50035. PLD. 2 hits. PS50195. PX. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLDP2_ARATH

Accession

Primary (citable) accession number: Q9M9W8
Secondary accession number(s): Q7XZQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	December 20, 2005
Last modified:	May 5, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)