Q9M9V6 (ICS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isochorismate synthase 2, chloroplastic
Short name=AtIcs2
EC=5.4.4.2

Alternative name(s):
Isochorismate mutase 2
menF-like protein 2

Gene names

Name:	ICS2
Ordered Locus Names:	At1g18870
ORF Names:	F6A14.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the synthesis of salicylic acid (SA) required for both local and systemic acquired resistance (LAR and SAR) while SA synthesized through the phenylalanine ammonium lyase (PAL) pathway seems to potentiate plant cell death. Also involved in phylloquinone (vitamin K1) synthesis. Has no isochorismate pyruvate lyase (IPL) activity. Ref.1 Ref.4
Catalytic activity	Chorismate = isochorismate.
Cofactor	Magnesium By similarity.
Pathway	Siderophore biosynthesis; salicylate biosynthesis.
Subcellular location	Plastid › chloroplast Ref.1.
Disruption phenotype	Plants do not show visible phenotype alteration due to the redundancy with ICS1 and are not impaired in salicylic acid accumulation upon induction. Double mutant ics1-ics2 is seedling lethal due to photosynthetic lesions induced by the lack of phylloquinone. Ref.1 Ref.4
Sequence similarities	Belongs to the isochorismate synthase family.
Sequence caution	The sequence AAF27094.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Magnesium
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW phylloquinone biosynthetic process Inferred from mutant phenotype Ref.4Ref.1. Source: TAIR salicylic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell plastid Inferred from direct assay Ref.1. Source: TAIR
Molecular_function	isochorismate synthase activity Inferred from mutant phenotype Ref.1. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9M9V6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 55	55	Chloroplast Potential
	Chain	56 – 562	507	Isochorismate synthase 2, chloroplastic		PRO_0000343792

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 05454B7F8124331C	FASTA	562	62,386
	10 20 30 40 50 60 MASLQCSFHF LGTNPKKYNP SSIFQSYSRT SFTKLSSRVS RQRFLRCTLS MNGCEADHKA 70 80 90 100 110 120 PLGTVETRTL STVPSPAAAT ERLITAVSDL KSQPPPFSSG IVRLQVPIEQ KIGAIDWLHA 130 140 150 160 170 180 QNEILPRSFF SRRSDSGRPD LLQDFSSDNG SSDHNPVSVA GIGSAVFFRD LDPFSHDDWR 190 200 210 220 230 240 SIRRFLSSKS PLIRAYGGLR FDPTGKIAVE WEHFGSFYFT VPQVEFDEFG GSSMLAATVA 250 260 270 280 290 300 WDNELSWTLE NAIEALQETM LQVSSVIMRL RRESLGVIVV SKNHVPSEGA YYPAVNNALE 310 320 330 340 350 360 IIKDKHSPLS KVVLARSSRI ITDTDIDPIA WLARLQCEGQ DAYQFCLQPP GAPAFIGNTP 370 380 390 400 410 420 ERLFHRKHLG VCSEALAATR PRGDSKVREM EIERDLLTSP KDDLEFSIVR ENIREKLKTI 430 440 450 460 470 480 CDRVVVKPHK SVRKLARVQH LYSQLAGQLK REDDEFNILT ALHPTPAVCG CPVEEARLLI 490 500 510 520 530 540 KQIESFDRGM YAGPIGFFGG GESEFSVGIR SALVEKGLGA LIYAGTGIVS GSNPSSEWNE 550 560 LELKISQFTK SLEHESALQP IN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and biological function of the ISOCHORISMATE SYNTHASE2 gene of Arabidopsis." Garcion C., Lohmann A., Lamodiere E., Catinot J., Buchala A., Doermann P., Metraux J.-P. Plant Physiol. 147:1279-1287(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"A plant locus essential for phylloquinone (vitamin K1) biosynthesis originated from a fusion of four eubacterial genes." Gross J., Cho W.K., Lezhneva L., Falk J., Krupinska K., Shinozaki K., Seki M., Herrmann R.G., Meurer J. J. Biol. Chem. 281:17189-17196(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU589462 mRNA. Translation: ACC60228.1. AC011809 Genomic DNA. Translation: AAF27094.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE29773.1.
IPI	IPI00544095.
PIR	F86322.
RefSeq	NP_001154351.1. NM_001160879.1. NP_173321.4. NM_101744.6.
UniGene	At.43418.
3D structure databases
ProteinModelPortal	Q9M9V6.
SMR	Q9M9V6. Positions 96-555.
ModBase	Search...
Proteomic databases
PaxDb	Q9M9V6.
PRIDE	Q9M9V6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G18870.1; AT1G18870.1; AT1G18870.
GeneID	838468.
KEGG	ath:AT1G18870.
Organism-specific databases
TAIR	At1g18870.
Phylogenomic databases
eggNOG	COG1169.
HOGENOM	HOG000006336.
InParanoid	Q9M9V6.
KO	K02552.
OMA	SEWNELE.
PhylomeDB	Q9M9V6.
ProtClustDB	PLN02786.
Enzyme and pathway databases
UniPathway	UPA00025.
Gene expression databases
ArrayExpress	Q9M9V6.
Genevestigator	Q9M9V6.
Family and domain databases
Gene3D	3.60.120.10. 1 hit.
InterPro	IPR005801. ADC_synthase. IPR015890. Chorismate-bd_C. IPR004561. IsoChor_synthase. [Graphical view]
PANTHER	PTHR11236:SF3. PTHR11236:SF3. 1 hit.
Pfam	PF00425. Chorismate_bind. 1 hit. [Graphical view]
SUPFAM	SSF56322. TRPE_1_chor_bd. 1 hit.
TIGRFAMs	TIGR00543. isochor_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ICS2_ARATH

Accession

Primary (citable) accession number: Q9M9V6
Secondary accession number(s): B2LWF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program