Q9M9S2 (PLY3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase 3
EC=4.2.2.2

Alternative name(s):
Pectate lyase A2

Gene names

Name:	AT59
Ordered Locus Names:	At1g14420
ORF Names:	F14L17.19

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Tissue specificity	Expressed in pollen, but not in leaves. Ref.1
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	plant-type cell wall organization Traceable author statement Ref.1. Source: TAIR
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 459	430	Probable pectate lyase 3		PRO_0000024867
Sites
	Active site	331	1	Potential
	Metal binding	251	1	Calcium By similarity
	Metal binding	275	1	Calcium By similarity
	Metal binding	279	1	Calcium By similarity
Amino acid modifications
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	258	1	F → I in AAB69759. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9M9S2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 634FBB54FEDCBA17	FASTA	459	51,455
	10 20 30 40 50 60 MAAAFLNLGG YVFVFFSSFL AIVAPQVRGN VAVFDSYWTQ RQSDALKQTI GSYDPHPLNV 70 80 90 100 110 120 TNHFNYHVNI AVDASESRND TRRELTQVRS GRKTHKSSGK CLAYNPIDNC WRCDRNWANN 130 140 150 160 170 180 RKKLADCVLG FGRRTTGGKD GPIYVVKDAS DNDLINPKPG TLRHAVTRDG PLWIIFARSM 190 200 210 220 230 240 IIKLQQELMI TSDKTIDGRG ARVYIMEGAG LTLQFVNNVI IHNIYVKHIV PGNGGLIRDS 250 260 270 280 290 300 EAHIGLRTKS DGDGISLFGA TNIWIDHVSM TRCADGMIDA IDGSTAVTIS NSHFTDHQEV 310 320 330 340 350 360 MLFGARDEHV IDKKMQITVA FNHFGKRLEQ RMPRCRYGTI HVVNNDYTHW EMYAIGGNMN 370 380 390 400 410 420 PTIISQGNRF IAPPNEEAKQ ITKREYTPYG EWKSWNWQSE GDYFLNGAYF VQSGKANAWS 430 440 450 SKPKTPLPNK FTIRPKPGTM VRKLTMDAGV LGCKLGEAC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the tobacco and Arabidopsis homologues of the pollen-expressed LAT59 gene of tomato." Kulikauskas R., McCormick S. Plant Mol. Biol. 34:809-814(1997) [PubMed: 9278171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83619 Genomic DNA. Translation: AAB69759.1. AC012188 Genomic DNA. Translation: AAF43942.1. CP002684 Genomic DNA. Translation: AEE29161.1. BT010451 mRNA. Translation: AAQ62871.1.
IPI	IPI00541066.
PIR	G86278.
RefSeq	NP_172894.1. NM_101309.4.
UniGene	At.41974.
3D structure databases
ProteinModelPortal	Q9M9S2.
SMR	Q9M9S2. Positions 104-452.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9M9S2.
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	Q9M9S2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G14420.1; AT1G14420.1; AT1G14420.
GeneID	838004.
GenomeReviews	Gene locus AT1G14420 in contig CT485782_GR.
KEGG	ath:AT1G14420.
NMPDR	fig|3702.1.peg.1725.
Organism-specific databases
TAIR	At1g14420.
Phylogenomic databases
eggNOG	euNOG10883.
GeneTree	EPGT00050000008990.
HOGENOM	HBG317381.
InParanoid	Q9M9S2.
OMA	TRRELTQ.
PhylomeDB	Q9M9S2.
ProtClustDB	CLSN2682943.
Gene expression databases
ArrayExpress	Q9M9S2.
Genevestigator	Q9M9S2.
GermOnline	AT1G14420. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR007524. Pec_lyase_N. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01728.
Pfam	PF00544. Pec_lyase_C. 1 hit. PF04431. Pec_lyase_N. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY3_ARATH

Accession

Primary (citable) accession number: Q9M9S2
Secondary accession number(s): O23665

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program