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Protein

UTP--glucose-1-phosphate uridylyltransferase 2

Gene

UGP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts glucose 1-phosphate to UDP-glucose, which is the major glycosyl donor for polysaccharides. Acts redundantly with UGP1 and is essential for the synthesis of sucrose, starch and cell wall, and callose deposition.2 Publications

Catalytic activityi

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99UTPCombined sources1 Publication1
Binding sitei162UTPCombined sources1 Publication1
Binding sitei191UTP; via amide nitrogenCombined sources1 Publication1
Binding sitei192SubstrateBy similarity1
Binding sitei222UTPCombined sources1 Publication1
Binding sitei360UTPCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

  • callose deposition in cell wall Source: TAIR
  • cellular response to phosphate starvation Source: TAIR
  • glycogen metabolic process Source: GO_Central
  • pollen development Source: TAIR
  • response to salt stress Source: TAIR
  • sucrose metabolic process Source: TAIR
  • UDP-glucose metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

BioCyciARA:AT3G03250-MONOMER.
MetaCyc:AT3G03250-MONOMER.
BRENDAi2.7.7.9. 399.
ReactomeiR-ATH-173599. Formation of the active cofactor, UDP-glucuronate.
R-ATH-3322077. Glycogen synthesis.
SABIO-RKQ9M9P3.

Names & Taxonomyi

Protein namesi
Recommended name:
UTP--glucose-1-phosphate uridylyltransferase 2 (EC:2.7.7.9)
Alternative name(s):
UDP-glucose pyrophosphorylase 2
Short name:
AtUGP21 Publication
Short name:
UDPGP 2
Short name:
UGPase 2
Gene namesi
Name:UGP21 Publication
Ordered Locus Names:At3g03250
ORF Names:T17B22.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G03250.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • plasma membrane Source: TAIR
  • pollen tube Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Reduced number of seeds (PubMed:19366709). The double mutants upg1 and ugp2 display severe growth defects and male sterility due to the absence of callose deposition around microspores (PubMed:20435647).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001857572 – 469UTP--glucose-1-phosphate uridylyltransferase 2Add BLAST468

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9M9P3.
PRIDEiQ9M9P3.

PTM databases

iPTMnetiQ9M9P3.

Expressioni

Tissue specificityi

Expressed in cauline leaves, flowers and siliques.1 Publication

Inductioni

By sucrose.1 Publication

Gene expression databases

GenevisibleiQ9M9P3. AT.

Interactioni

Protein-protein interaction databases

BioGridi6646. 2 interactors.
STRINGi3702.AT3G03250.1.

Structurei

Secondary structure

1469
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 17Combined sources10
Beta strandi19 – 21Combined sources3
Helixi23 – 36Combined sources14
Helixi46 – 48Combined sources3
Turni54 – 56Combined sources3
Beta strandi57 – 59Combined sources3
Helixi60 – 62Combined sources3
Helixi70 – 77Combined sources8
Beta strandi80 – 86Combined sources7
Helixi91 – 93Combined sources3
Beta strandi96 – 98Combined sources3
Helixi99 – 101Combined sources3
Beta strandi102 – 105Combined sources4
Helixi110 – 125Combined sources16
Beta strandi131 – 135Combined sources5
Turni137 – 139Combined sources3
Helixi140 – 147Combined sources8
Helixi148 – 150Combined sources3
Beta strandi153 – 155Combined sources3
Beta strandi157 – 161Combined sources5
Turni170 – 173Combined sources4
Helixi176 – 179Combined sources4
Helixi184 – 186Combined sources3
Helixi192 – 194Combined sources3
Helixi195 – 202Combined sources8
Helixi204 – 209Combined sources6
Beta strandi215 – 220Combined sources6
Helixi230 – 239Combined sources10
Beta strandi242 – 249Combined sources8
Helixi252 – 254Combined sources3
Beta strandi259 – 263Combined sources5
Beta strandi266 – 270Combined sources5
Helixi272 – 274Combined sources3
Helixi277 – 279Combined sources3
Helixi280 – 284Combined sources5
Beta strandi285 – 288Combined sources4
Beta strandi291 – 300Combined sources10
Helixi301 – 309Combined sources9
Beta strandi321 – 324Combined sources4
Beta strandi327 – 330Combined sources4
Helixi336 – 342Combined sources7
Beta strandi347 – 350Combined sources4
Helixi353 – 355Combined sources3
Helixi362 – 369Combined sources8
Beta strandi373 – 376Combined sources4
Beta strandi379 – 382Combined sources4
Beta strandi388 – 390Combined sources3
Beta strandi393 – 396Combined sources4
Helixi398 – 400Combined sources3
Helixi403 – 408Combined sources6
Beta strandi410 – 412Combined sources3
Beta strandi419 – 430Combined sources12
Beta strandi435 – 443Combined sources9
Beta strandi449 – 452Combined sources4
Beta strandi457 – 460Combined sources4
Helixi466 – 468Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z90X-ray1.86A/B1-469[»]
2ICXX-ray1.85A/B2-469[»]
2ICYX-ray1.64A/B2-469[»]
2Q4JX-ray1.86A/B1-469[»]
ProteinModelPortaliQ9M9P3.
SMRiQ9M9P3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9M9P3.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 88UTP bindingCombined sources1 Publication4
Regioni87 – 88Substrate bindingBy similarity2
Regioni220 – 222Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

eggNOGiKOG2638. Eukaryota.
COG4284. LUCA.
HOGENOMiHOG000113618.
InParanoidiQ9M9P3.
KOiK00963.
OMAiFNTHEDS.
OrthoDBiEOG09360DL3.
PhylomeDBiQ9M9P3.

Family and domain databases

CDDicd00897. UGPase_euk. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
IPR016267. UDPGP_trans.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M9P3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATTENLPQ LKSAVDGLTE MSESEKSGFI SLVSRYLSGE AQHIEWSKIQ
60 70 80 90 100
TPTDEIVVPY EKMTPVSQDV AETKNLLDKL VVLKLNGGLG TTMGCTGPKS
110 120 130 140 150
VIEVRDGLTF LDLIVIQIEN LNNKYGCKVP LVLMNSFNTH DDTHKIVEKY
160 170 180 190 200
TNSNVDIHTF NQSKYPRVVA DEFVPWPSKG KTDKEGWYPP GHGDVFPALM
210 220 230 240 250
NSGKLDTFLS QGKEYVFVAN SDNLGAIVDL TILKHLIQNK NEYCMEVTPK
260 270 280 290 300
TLADVKGGTL ISYEGKVQLL EIAQVPDEHV NEFKSIEKFK IFNTNNLWVN
310 320 330 340 350
LKAIKKLVEA DALKMEIIPN PKEVDGVKVL QLETAAGAAI RFFDNAIGVN
360 370 380 390 400
VPRSRFLPVK ASSDLLLVQS DLYTLVDGFV TRNKARTNPS NPSIELGPEF
410 420 430 440 450
KKVATFLSRF KSIPSIVELD SLKVSGDVWF GSSIVLKGKV TVAAKSGVKL
460
EIPDRAVVEN KNINGPEDL
Length:469
Mass (Da):51,738
Last modified:October 1, 2000 - v1
Checksum:iD0B70B92A2372820
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti187W → R in AAK32773 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012328 Genomic DNA. Translation: AAF26102.1.
CP002686 Genomic DNA. Translation: AEE73917.1.
AY035071 mRNA. Translation: AAK59576.1.
AY059148 mRNA. Translation: AAL15254.1.
AF361605 mRNA. Translation: AAK32773.1.
RefSeqiNP_186975.1. NM_111195.4.
UniGeneiAt.24250.

Genome annotation databases

EnsemblPlantsiAT3G03250.1; AT3G03250.1; AT3G03250.
GeneIDi821313.
GrameneiAT3G03250.1; AT3G03250.1; AT3G03250.
KEGGiath:AT3G03250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012328 Genomic DNA. Translation: AAF26102.1.
CP002686 Genomic DNA. Translation: AEE73917.1.
AY035071 mRNA. Translation: AAK59576.1.
AY059148 mRNA. Translation: AAL15254.1.
AF361605 mRNA. Translation: AAK32773.1.
RefSeqiNP_186975.1. NM_111195.4.
UniGeneiAt.24250.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z90X-ray1.86A/B1-469[»]
2ICXX-ray1.85A/B2-469[»]
2ICYX-ray1.64A/B2-469[»]
2Q4JX-ray1.86A/B1-469[»]
ProteinModelPortaliQ9M9P3.
SMRiQ9M9P3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6646. 2 interactors.
STRINGi3702.AT3G03250.1.

PTM databases

iPTMnetiQ9M9P3.

Proteomic databases

PaxDbiQ9M9P3.
PRIDEiQ9M9P3.

Protocols and materials databases

DNASUi821313.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G03250.1; AT3G03250.1; AT3G03250.
GeneIDi821313.
GrameneiAT3G03250.1; AT3G03250.1; AT3G03250.
KEGGiath:AT3G03250.

Organism-specific databases

TAIRiAT3G03250.

Phylogenomic databases

eggNOGiKOG2638. Eukaryota.
COG4284. LUCA.
HOGENOMiHOG000113618.
InParanoidiQ9M9P3.
KOiK00963.
OMAiFNTHEDS.
OrthoDBiEOG09360DL3.
PhylomeDBiQ9M9P3.

Enzyme and pathway databases

BioCyciARA:AT3G03250-MONOMER.
MetaCyc:AT3G03250-MONOMER.
BRENDAi2.7.7.9. 399.
ReactomeiR-ATH-173599. Formation of the active cofactor, UDP-glucuronate.
R-ATH-3322077. Glycogen synthesis.
SABIO-RKQ9M9P3.

Miscellaneous databases

EvolutionaryTraceiQ9M9P3.
PROiQ9M9P3.

Gene expression databases

GenevisibleiQ9M9P3. AT.

Family and domain databases

CDDicd00897. UGPase_euk. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
IPR016267. UDPGP_trans.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 1 hit.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
PIRSFiPIRSF000806. UDPGP. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUGPA2_ARATH
AccessioniPrimary (citable) accession number: Q9M9P3
Secondary accession number(s): Q9ASY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.