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Protein

Lon protease homolog 4, chloroplastic/mitochondrial

Gene

LON4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei846 – 8461UniRule annotation
Active sitei889 – 8891UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi456 – 4638ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. ATP-dependent peptidase activity Source: UniProtKB-HAMAP
  3. sequence-specific DNA binding Source: UniProtKB-HAMAP
  4. serine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular response to oxidative stress Source: UniProtKB-HAMAP
  2. chaperone-mediated protein complex assembly Source: UniProtKB-HAMAP
  3. misfolded or incompletely synthesized protein catabolic process Source: UniProtKB-HAMAP
  4. oxidation-dependent protein catabolic process Source: UniProtKB-HAMAP
  5. regulation of mitochondrial DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G05790-MONOMER.

Protein family/group databases

MEROPSiS16.A02.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon protease homolog 4, chloroplastic/mitochondrial (EC:3.4.21.-UniRule annotation)
Short name:
AtLon4
Gene namesi
Name:LON4
Ordered Locus Names:At3g05790
ORF Names:F10A16.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G05790.

Subcellular locationi

Mitochondrion matrix UniRule annotation1 Publication. Plastidchloroplast thylakoid membrane 1 Publication; Peripheral membrane protein 1 Publication; Stromal side 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast thylakoid membrane Source: UniProtKB-SubCell
  3. mitochondrial matrix Source: UniProtKB-SubCell
  4. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 942Lon protease homolog 4, chloroplastic/mitochondrialPRO_0000045425
Transit peptidei1 – ?Chloroplast and mitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 541PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9M9L7.
PRIDEiQ9M9L7.

Expressioni

Gene expression databases

GenevestigatoriQ9M9L7.

Interactioni

Subunit structurei

Homohexamer or homoheptamer. Organized in a ring with a central cavity.UniRule annotation

Protein-protein interaction databases

STRINGi3702.AT3G05790.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9M9L7.
SMRiQ9M9L7. Positions 354-662, 725-938.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 299221LonUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0466.
HOGENOMiHOG000261409.
InParanoidiQ9M9L7.
KOiK08675.
OMAiGSTLMIE.
PhylomeDBiQ9M9L7.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03120. lonm_euk.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR027503. Lonm_euk.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS01046. LON_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M9L7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKFLTPTAY ASHHVTPATR FRSTPVKNLL FKQLTLLTGW NRSSYELGRR
60 70 80 90 100
SFSSDLDSDT KSSTTTVSAK PHLDDCLTVI ALPLPHKPLI PGFYMPIYVK
110 120 130 140 150
DPKVLAALQE SRRQQAPYAG AFLLKDDASS DSSSSSETEN ILEKLKGKEL
160 170 180 190 200
INRIHEVGTL AQISSIQGEQ VILIGHRQLR ITEMVSESED PLTVKVDHLK
210 220 230 240 250
DKPYDKDDDV IKATYFQVMS TLRDVLKTTS LWRDHVRTYT QACSLHIWHC
260 270 280 290 300
LRHIGEFNYP KLADFGAGIS GANKHQNQGV LEELDVHKRL ELTLELVKKE
310 320 330 340 350
VEINKIQESI AKAVEEKFSG DRRRIILKEQ INAIKKELGG ETDSKSALSE
360 370 380 390 400
KFRGRIDPIK DKIPGHVLKV IEEELKKLQL LETSSSEFDV TCNYLDWLTV
410 420 430 440 450
LPWGNFSDEN FNVLRAEKIL DEDHYGLSDV KERILEFIAV GGLRGTSQGK
460 470 480 490 500
IICLSGPTGV GKTSIGRSIA RALDRKFFRF SVGGLSDVAE IKGHRRTYIG
510 520 530 540 550
AMPGKMVQCL KNVGTENPLV LIDEIDKLGV RGHHGDPASA MLELLDPEQN
560 570 580 590 600
ANFLDHYLDV PIDLSKVLFV CTANVTDTIP GPLLDRMEVI TLSGYITDEK
610 620 630 640 650
MHIARDYLEK TARRDCGIKP EQVDVSDAAF LSLIEHYCRE AGVRNLQKQI
660 670 680 690 700
EKIFRKIALK LVRKAASTEV PRISDDVTTD TEETKSLAKT DLESPETSAE
710 720 730 740 750
GSTVLTDELA TGDPTESTTE QSGEVAETVE KYMIDESNLS DYVGKPVFQE
760 770 780 790 800
EKIYEQTPVG VVMGLAWTSM GGSTLYIETT FVEEGEGKGG LHITGRLGDV
810 820 830 840 850
MKESAEIAHT VARRIMLEKE PENKLFANSK LHLHVPAGAT PKDGPSAGCT
860 870 880 890 900
MITSLLSLAL KKPVRKDLAM TGEVTLTGRI LAIGGVKEKT IAARRSQVKV
910 920 930 940
IIFPEANRRD FDELARNVKE GLEVHFVDEY EQIFELAFGY DH
Length:942
Mass (Da):104,925
Last modified:October 1, 2000 - v1
Checksum:i90F3238714F4C52E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012393 Genomic DNA. Translation: AAF26081.1.
CP002686 Genomic DNA. Translation: AEE74297.1.
RefSeqiNP_566259.1. NM_111453.1.
UniGeneiAt.53196.

Genome annotation databases

EnsemblPlantsiAT3G05790.1; AT3G05790.1; AT3G05790.
GeneIDi819748.
KEGGiath:AT3G05790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC012393 Genomic DNA. Translation: AAF26081.1.
CP002686 Genomic DNA. Translation: AEE74297.1.
RefSeqiNP_566259.1. NM_111453.1.
UniGeneiAt.53196.

3D structure databases

ProteinModelPortaliQ9M9L7.
SMRiQ9M9L7. Positions 354-662, 725-938.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G05790.1-P.

Protein family/group databases

MEROPSiS16.A02.

Proteomic databases

PaxDbiQ9M9L7.
PRIDEiQ9M9L7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G05790.1; AT3G05790.1; AT3G05790.
GeneIDi819748.
KEGGiath:AT3G05790.

Organism-specific databases

GeneFarmi1770. 137.
TAIRiAT3G05790.

Phylogenomic databases

eggNOGiCOG0466.
HOGENOMiHOG000261409.
InParanoidiQ9M9L7.
KOiK08675.
OMAiGSTLMIE.
PhylomeDBiQ9M9L7.

Enzyme and pathway databases

BioCyciARA:AT3G05790-MONOMER.

Gene expression databases

GenevestigatoriQ9M9L7.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_03120. lonm_euk.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR027503. Lonm_euk.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR003111. Pept_S16_N.
IPR008268. Peptidase_S16_AS.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF02190. LON. 1 hit.
PF05362. Lon_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS01046. LON_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Multiple intracellular locations of Lon protease in Arabidopsis: evidence for the localization of AtLon4 to chloroplasts."
    Ostersetzer O., Kato Y., Adam Z., Sakamoto W.
    Plant Cell Physiol. 48:881-885(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiLONM4_ARATH
AccessioniPrimary (citable) accession number: Q9M9L7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: October 1, 2000
Last modified: March 4, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.