Q9M9L7 (LONM4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lon protease homolog 4, chloroplastic/mitochondrial
Short name=AtLon4
EC=3.4.21.-

Gene names

Name:	LON4
Ordered Locus Names:	At3g05790
ORF Names:	F10A16.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	942 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner By similarity. HAMAP-Rule MF_03120
Subunit structure	Homohexamer or homoheptamer. Organized in a ring with a central cavity By similarity.
Subcellular location	Mitochondrion matrix. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side Ref.3.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Mitochondrion Plastid Thylakoid
Domain	Transit peptide
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to oxidative stress Inferred from Biological aspect of Ancestor. Source: RefGenome chaperone-mediated protein complex assembly Inferred from Biological aspect of Ancestor. Source: RefGenome misfolded or incompletely synthesized protein catabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome mitochondrion organization Inferred from Biological aspect of Ancestor. Source: RefGenome oxidation-dependent protein catabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome protein homooligomerization Inferred from Biological aspect of Ancestor. Source: RefGenome response to hypoxia Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	chloroplast Inferred from direct assay Ref.3. Source: TAIR chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial nucleoid Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	ATP binding Inferred from Biological aspect of Ancestor. Source: RefGenome ATP-dependent peptidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome mitochondrial light strand promoter anti-sense binding Inferred from Biological aspect of Ancestor. Source: RefGenome serine-type endopeptidase activity Inferred from Biological aspect of Ancestor. Source: RefGenome single-stranded DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome single-stranded RNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast and mitochondrion Potential
	Chain	? – 942		Lon protease homolog 4, chloroplastic/mitochondrial HAMAP-Rule MF_03120		PRO_0000045425
Regions
	Domain	79 – 299	221	Lon
	Nucleotide binding	456 – 463	8	ATP By similarity
Sites
	Active site	846	1	By similarity
	Active site	889	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9M9L7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 90F3238714F4C52E	FASTA	942	104,925
	10 20 30 40 50 60 MLKFLTPTAY ASHHVTPATR FRSTPVKNLL FKQLTLLTGW NRSSYELGRR SFSSDLDSDT 70 80 90 100 110 120 KSSTTTVSAK PHLDDCLTVI ALPLPHKPLI PGFYMPIYVK DPKVLAALQE SRRQQAPYAG 130 140 150 160 170 180 AFLLKDDASS DSSSSSETEN ILEKLKGKEL INRIHEVGTL AQISSIQGEQ VILIGHRQLR 190 200 210 220 230 240 ITEMVSESED PLTVKVDHLK DKPYDKDDDV IKATYFQVMS TLRDVLKTTS LWRDHVRTYT 250 260 270 280 290 300 QACSLHIWHC LRHIGEFNYP KLADFGAGIS GANKHQNQGV LEELDVHKRL ELTLELVKKE 310 320 330 340 350 360 VEINKIQESI AKAVEEKFSG DRRRIILKEQ INAIKKELGG ETDSKSALSE KFRGRIDPIK 370 380 390 400 410 420 DKIPGHVLKV IEEELKKLQL LETSSSEFDV TCNYLDWLTV LPWGNFSDEN FNVLRAEKIL 430 440 450 460 470 480 DEDHYGLSDV KERILEFIAV GGLRGTSQGK IICLSGPTGV GKTSIGRSIA RALDRKFFRF 490 500 510 520 530 540 SVGGLSDVAE IKGHRRTYIG AMPGKMVQCL KNVGTENPLV LIDEIDKLGV RGHHGDPASA 550 560 570 580 590 600 MLELLDPEQN ANFLDHYLDV PIDLSKVLFV CTANVTDTIP GPLLDRMEVI TLSGYITDEK 610 620 630 640 650 660 MHIARDYLEK TARRDCGIKP EQVDVSDAAF LSLIEHYCRE AGVRNLQKQI EKIFRKIALK 670 680 690 700 710 720 LVRKAASTEV PRISDDVTTD TEETKSLAKT DLESPETSAE GSTVLTDELA TGDPTESTTE 730 740 750 760 770 780 QSGEVAETVE KYMIDESNLS DYVGKPVFQE EKIYEQTPVG VVMGLAWTSM GGSTLYIETT 790 800 810 820 830 840 FVEEGEGKGG LHITGRLGDV MKESAEIAHT VARRIMLEKE PENKLFANSK LHLHVPAGAT 850 860 870 880 890 900 PKDGPSAGCT MITSLLSLAL KKPVRKDLAM TGEVTLTGRI LAIGGVKEKT IAARRSQVKV 910 920 930 940 IIFPEANRRD FDELARNVKE GLEVHFVDEY EQIFELAFGY DH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Multiple intracellular locations of Lon protease in Arabidopsis: evidence for the localization of AtLon4 to chloroplasts." Ostersetzer O., Kato Y., Adam Z., Sakamoto W. Plant Cell Physiol. 48:881-885(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC012393 Genomic DNA. Translation: AAF26081.1. CP002686 Genomic DNA. Translation: AEE74297.1.
IPI	IPI00528748.
RefSeq	NP_566259.1. NM_111453.1.
UniGene	At.53196.
3D structure databases
ProteinModelPortal	Q9M9L7.
SMR	Q9M9L7. Positions 354-662, 725-938.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G05790.1-P.
Protein family/group databases
MEROPS	S16.A02.
Proteomic databases
PaxDb	Q9M9L7.
PRIDE	Q9M9L7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G05790.1; AT3G05790.1; AT3G05790.
GeneID	819748.
KEGG	ath:AT3G05790.
Organism-specific databases
GeneFarm	1770. 137.
TAIR	At3g05790.
Phylogenomic databases
eggNOG	COG0466.
HOGENOM	HOG000261409.
InParanoid	Q9M9L7.
KO	K08675.
OMA	FFILTAN.
PhylomeDB	Q9M9L7.
ProtClustDB	CLSN2917104.
Gene expression databases
Genevestigator	Q9M9L7.
GermOnline	AT3G05790. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_03120. lonm_euk.
InterPro	IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR027065. Lon_Prtase. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR015947. PUA-like_domain. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR10046. PTHR10046. 1 hit.
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PIRSF	PIRSF001174. Lon_proteas. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
SUPFAM	SSF88697. PUA-like. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LONM4_ARATH

Accession

Primary (citable) accession number: Q9M9L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program