ID   CK1_ARATH               Reviewed;         346 AA.
AC   Q9M9H6; Q9C9J3;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   27-MAR-2024, entry version 147.
DE   RecName: Full=Probable choline kinase 1;
DE            Short=AtCK1;
DE            EC=2.7.1.32;
GN   Name=CK1; Synonyms=CK; OrderedLocusNames=At1g71697;
GN   ORFNames=F14O23.8, F26A9.3;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   TISSUE SPECIFICITY, AND INDUCTION BY WOUNDING.
RX   PubMed=9342878; DOI=10.1104/pp.115.2.817;
RA   Titarenko E., Rojo E., Leon J., Sanchez-Serrano J.J.;
RT   "Jasmonic acid-dependent and -independent signaling pathways control wound-
RT   induced gene activation in Arabidopsis thaliana.";
RL   Plant Physiol. 115:817-826(1997).
RN   [6]
RP   INDUCTION.
RX   PubMed=15147879; DOI=10.1016/j.febslet.2004.04.015;
RA   Tasseva G., Richard L., Zachowski A.;
RT   "Regulation of phosphatidylcholine biosynthesis under salt stress involves
RT   choline kinases in Arabidopsis thaliana.";
RL   FEBS Lett. 566:115-120(2004).
CC   -!- FUNCTION: Involved in phospholipid biosynthesis. Catalyzes the first
CC       step in phosphatidylcholine biosynthesis (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + choline = ADP + H(+) + phosphocholine;
CC         Xref=Rhea:RHEA:12837, ChEBI:CHEBI:15354, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:295975, ChEBI:CHEBI:456216;
CC         EC=2.7.1.32;
CC   -!- PATHWAY: Phospholipid metabolism; phosphatidylcholine biosynthesis;
CC       phosphocholine from choline: step 1/1.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. Expressed at low levels in
CC       cauline leaves and flowers. {ECO:0000269|PubMed:9342878}.
CC   -!- INDUCTION: By wounding, and salt and osmotic stresses.
CC       {ECO:0000269|PubMed:15147879, ECO:0000269|PubMed:9342878}.
CC   -!- SIMILARITY: Belongs to the choline/ethanolamine kinase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG51828.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC012654; AAF43223.1; -; Genomic_DNA.
DR   EMBL; AC016163; AAG51828.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE35220.1; -; Genomic_DNA.
DR   EMBL; BT008311; AAP37670.1; -; mRNA.
DR   EMBL; AK229787; BAF01619.1; -; mRNA.
DR   EMBL; AK230407; BAF02205.1; -; mRNA.
DR   PIR; C96739; C96739.
DR   RefSeq; NP_177315.1; NM_105828.4.
DR   AlphaFoldDB; Q9M9H6; -.
DR   SMR; Q9M9H6; -.
DR   BioGRID; 28720; 1.
DR   STRING; 3702.Q9M9H6; -.
DR   iPTMnet; Q9M9H6; -.
DR   PaxDb; 3702-AT1G71697-1; -.
DR   ProteomicsDB; 246868; -.
DR   EnsemblPlants; AT1G71697.1; AT1G71697.1; AT1G71697.
DR   GeneID; 843500; -.
DR   Gramene; AT1G71697.1; AT1G71697.1; AT1G71697.
DR   KEGG; ath:AT1G71697; -.
DR   Araport; AT1G71697; -.
DR   TAIR; AT1G71697; CK1.
DR   eggNOG; KOG2686; Eukaryota.
DR   HOGENOM; CLU_012712_0_2_1; -.
DR   InParanoid; Q9M9H6; -.
DR   OMA; YECKPRL; -.
DR   OrthoDB; 144299at2759; -.
DR   PhylomeDB; Q9M9H6; -.
DR   BioCyc; ARA:AT1G71697-MONOMER; -.
DR   BRENDA; 2.7.1.32; 399.
DR   UniPathway; UPA00753; UER00737.
DR   PRO; PR:Q9M9H6; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9M9H6; baseline and differential.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004103; F:choline kinase activity; ISS:TAIR.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR   CDD; cd05157; ETNK_euk; 1.
DR   Gene3D; 3.90.1200.10; -; 1.
DR   InterPro; IPR007521; Choline_kin_N.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   PANTHER; PTHR22603; CHOLINE/ETHANOALAMINE KINASE; 1.
DR   PANTHER; PTHR22603:SF93; RE24176P; 1.
DR   Pfam; PF04428; Choline_kin_N; 1.
DR   Pfam; PF01633; Choline_kinase; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR   Genevisible; Q9M9H6; AT.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Lipid biosynthesis; Lipid metabolism;
KW   Nucleotide-binding; Phospholipid biosynthesis; Phospholipid metabolism;
KW   Reference proteome; Transferase.
FT   CHAIN           1..346
FT                   /note="Probable choline kinase 1"
FT                   /id="PRO_0000423346"
FT   BINDING         73
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         210
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         227
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   346 AA;  40016 MW;  3FABBAAAB09B5597 CRC64;
     MAIKTKTSLI PSCSSPEDLK RVLQTLGSSW GDVVEDLERL EVVPLKGAMT NEVYQINWPT
     LNGEDVHRKV LVRIYGDGVD LFFNRGDEIK TFECMSHHGY GPKLLGRFSD GRLEEFIHAR
     TLSADDLRVA ETSDFIAAKL REFHKLDMPG PKNVLLWERL RTWLKEAKNL ASPIEMDKYR
     LEGLENEINL LEERLTRDDQ EIGFCHNDLQ YGNVMIDEVT NAITIIDYEY SSFNPIAYDI
     ANHFCEMAAN YHSDTPHVLD YTLYPGEGER RRFISTYLGS TGNATSDKEV ERLLKDAESY
     TLANHIFWGL WGIISGHVNK IEFDYMEYAR QRFEQYWLRK PLLLEG
//