Reviewed, UniProtKB/Swiss-Prot Q9M995 (GUN5_ARATH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 5
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 5

Gene names

Ordered Locus Names:	At1g48930
ORF Names:	F27J15.28, F27K7.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	627 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 627	601	Endoglucanase 5		PRO_0000249258
Sites
	Active site	416	1	By similarity
	Active site	468	1	By similarity
	Active site	477	1	By similarity
Amino acid modifications
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential
	Glycosylation	561	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9M995-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 271C44CB63F06644	FASTA	627	69,080
	10 20 30 40 50 60 MRKFGGSLFG VSLLLSVLLA AATAAAEYYN YGSALDKTFL FFEAQRSGKL PAAQRVKWRG 70 80 90 100 110 120 PSGLKDGLAQ GVSLEGGYYD AGDHVKFGLP MAFAVTMLSW AAVDNRKELS SSNQMQQTLW 130 140 150 160 170 180 SIRWGTDYFI KAHPQPNVLW GQVGDGESDH YCWERPEDMT TSRTAYKLDP YHPGSDLAGE 190 200 210 220 230 240 TAAALAAASL AFKPFNSSYS ALLLSHAKEL FSFADKYRGL YTNSIPNAKA FYMSSGYSDE 250 260 270 280 290 300 LLWAAAWLHR ATGDQYYLKY AMDNSGYMGG TGWGVKEFSW DNKYAGVQIL LSKILLEGKG 310 320 330 340 350 360 GIYTSTLKQY QTKADYFACA CLKKNGGYNI QTTPGGLMYV REWNNLQYAS AAAYLLAVYS 370 380 390 400 410 420 DYLSAANAKL NCPDGLVQPQ GLLDFARSQA DYILGKNRQG MSYVVGYGPK YPIRVHHRGS 430 440 450 460 470 480 SIPSIFAQRS SVSCVQGFDS WYRRSQGDPN VIYGALVGGP DENDNYSDDR SNYEQSEPTL 490 500 510 520 530 540 SGTAPLVGLF AKLYGGSLGS YGGGSYKPYE TTKPAASSYK ATPTTYSPKQ SGAQIEFLHS 550 560 570 580 590 600 ITSNWIAGNT RYYRHKVIIK NNSQKPISDL KLKIEDLSGP IWGLNPTGQK YTYQLPQWQK 610 620 TLRAGQAYDF VYVQGGPQAK VSVLSYN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC016041 Genomic DNA. Translation: AAF69707.1. BT002935 mRNA. Translation: AAO22749.1. BT005638 mRNA. Translation: AAO64058.1.
IPI	IPI00519615.
PIR	B96527.
RefSeq	NP_175323.1.
UniGene	At.16674
3D structure databases
HSSP	HSSP built from PDB template 1KS8 based on UniProtKB O77044.
ModBase	Search...
Protein family/group databases
CAZy	CBM49. Carbohydrate-Binding Module Family 49. GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	Q9M995.
Genome annotation databases
GeneID	841315.
GenomeReviews	Gene locus AT1G48930 in contig CT485782_GR.
KEGG	ath:AT1G48930.
NMPDR	fig|3702.1.peg.4367.
Organism-specific databases
TAIR	At1g48930.
Phylogenomic databases
OMA	Q9M995. DAYVDAN.
Enzyme and pathway databases
BRENDA	3.2.1.4. 302.
Gene expression databases
ArrayExpress	Q9M995.
GermOnline	AT1G48930. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR019028. CBM_49. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF09478. CBM49. 1 hit. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN5_ARATH

Accession

Primary (citable) accession number: Q9M995

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)