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Protein

Endoglucanase 5

Gene

At1g48930

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei416 – 4161PROSITE-ProRule annotation
Active sitei468 – 4681PROSITE-ProRule annotation
Active sitei477 – 4771PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciARA:AT1G48930-MONOMER.

Protein family/group databases

CAZyiCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 5 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 5
Gene namesi
Ordered Locus Names:At1g48930
ORF Names:F27J15.28, F27K7.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G48930.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 627601Endoglucanase 5PRO_0000249258Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi561 – 5611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9M995.
PRIDEiQ9M995.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G48930.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M995.
SMRiQ9M995. Positions 29-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG05134.
HOGENOMiHOG000021033.
InParanoidiQ9M995.
OMAiQGGPQAK.
PhylomeDBiQ9M995.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01063. CBM49. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKFGGSLFG VSLLLSVLLA AATAAAEYYN YGSALDKTFL FFEAQRSGKL
60 70 80 90 100
PAAQRVKWRG PSGLKDGLAQ GVSLEGGYYD AGDHVKFGLP MAFAVTMLSW
110 120 130 140 150
AAVDNRKELS SSNQMQQTLW SIRWGTDYFI KAHPQPNVLW GQVGDGESDH
160 170 180 190 200
YCWERPEDMT TSRTAYKLDP YHPGSDLAGE TAAALAAASL AFKPFNSSYS
210 220 230 240 250
ALLLSHAKEL FSFADKYRGL YTNSIPNAKA FYMSSGYSDE LLWAAAWLHR
260 270 280 290 300
ATGDQYYLKY AMDNSGYMGG TGWGVKEFSW DNKYAGVQIL LSKILLEGKG
310 320 330 340 350
GIYTSTLKQY QTKADYFACA CLKKNGGYNI QTTPGGLMYV REWNNLQYAS
360 370 380 390 400
AAAYLLAVYS DYLSAANAKL NCPDGLVQPQ GLLDFARSQA DYILGKNRQG
410 420 430 440 450
MSYVVGYGPK YPIRVHHRGS SIPSIFAQRS SVSCVQGFDS WYRRSQGDPN
460 470 480 490 500
VIYGALVGGP DENDNYSDDR SNYEQSEPTL SGTAPLVGLF AKLYGGSLGS
510 520 530 540 550
YGGGSYKPYE TTKPAASSYK ATPTTYSPKQ SGAQIEFLHS ITSNWIAGNT
560 570 580 590 600
RYYRHKVIIK NNSQKPISDL KLKIEDLSGP IWGLNPTGQK YTYQLPQWQK
610 620
TLRAGQAYDF VYVQGGPQAK VSVLSYN
Length:627
Mass (Da):69,080
Last modified:October 1, 2000 - v1
Checksum:i271C44CB63F06644
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016041 Genomic DNA. Translation: AAF69707.1.
CP002684 Genomic DNA. Translation: AEE32368.1.
BT002935 mRNA. Translation: AAO22749.1.
BT005638 mRNA. Translation: AAO64058.1.
PIRiB96527.
RefSeqiNP_175323.1. NM_103786.2.
UniGeneiAt.16674.

Genome annotation databases

EnsemblPlantsiAT1G48930.1; AT1G48930.1; AT1G48930.
GeneIDi841315.
KEGGiath:AT1G48930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC016041 Genomic DNA. Translation: AAF69707.1.
CP002684 Genomic DNA. Translation: AEE32368.1.
BT002935 mRNA. Translation: AAO22749.1.
BT005638 mRNA. Translation: AAO64058.1.
PIRiB96527.
RefSeqiNP_175323.1. NM_103786.2.
UniGeneiAt.16674.

3D structure databases

ProteinModelPortaliQ9M995.
SMRiQ9M995. Positions 29-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G48930.1.

Protein family/group databases

CAZyiCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbiQ9M995.
PRIDEiQ9M995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G48930.1; AT1G48930.1; AT1G48930.
GeneIDi841315.
KEGGiath:AT1G48930.

Organism-specific databases

TAIRiAT1G48930.

Phylogenomic databases

eggNOGiNOG05134.
HOGENOMiHOG000021033.
InParanoidiQ9M995.
OMAiQGGPQAK.
PhylomeDBiQ9M995.

Enzyme and pathway databases

BioCyciARA:AT1G48930-MONOMER.

Miscellaneous databases

PROiQ9M995.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTiSM01063. CBM49. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
    Libertini E., Li Y., McQueen-Mason S.J.
    J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.

Entry informationi

Entry nameiGUN5_ARATH
AccessioniPrimary (citable) accession number: Q9M995
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2000
Last modified: June 24, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.