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Q9M995 (GUN5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 5

EC=3.2.1.4
Alternative name(s):
Endo-1,4-beta glucanase 5
Gene names
Ordered Locus Names:At1g48930
ORF Names:F27J15.28, F27K7.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length627 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cell wall biogenesis/degradation
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

cellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 627601Endoglucanase 5
PRO_0000249258

Sites

Active site4161 By similarity
Active site4681 By similarity
Active site4771 By similarity

Amino acid modifications

Glycosylation1961N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation5611N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9M995 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 271C44CB63F06644

FASTA62769,080
        10         20         30         40         50         60 
MRKFGGSLFG VSLLLSVLLA AATAAAEYYN YGSALDKTFL FFEAQRSGKL PAAQRVKWRG 

        70         80         90        100        110        120 
PSGLKDGLAQ GVSLEGGYYD AGDHVKFGLP MAFAVTMLSW AAVDNRKELS SSNQMQQTLW 

       130        140        150        160        170        180 
SIRWGTDYFI KAHPQPNVLW GQVGDGESDH YCWERPEDMT TSRTAYKLDP YHPGSDLAGE 

       190        200        210        220        230        240 
TAAALAAASL AFKPFNSSYS ALLLSHAKEL FSFADKYRGL YTNSIPNAKA FYMSSGYSDE 

       250        260        270        280        290        300 
LLWAAAWLHR ATGDQYYLKY AMDNSGYMGG TGWGVKEFSW DNKYAGVQIL LSKILLEGKG 

       310        320        330        340        350        360 
GIYTSTLKQY QTKADYFACA CLKKNGGYNI QTTPGGLMYV REWNNLQYAS AAAYLLAVYS 

       370        380        390        400        410        420 
DYLSAANAKL NCPDGLVQPQ GLLDFARSQA DYILGKNRQG MSYVVGYGPK YPIRVHHRGS 

       430        440        450        460        470        480 
SIPSIFAQRS SVSCVQGFDS WYRRSQGDPN VIYGALVGGP DENDNYSDDR SNYEQSEPTL 

       490        500        510        520        530        540 
SGTAPLVGLF AKLYGGSLGS YGGGSYKPYE TTKPAASSYK ATPTTYSPKQ SGAQIEFLHS 

       550        560        570        580        590        600 
ITSNWIAGNT RYYRHKVIIK NNSQKPISDL KLKIEDLSGP IWGLNPTGQK YTYQLPQWQK 

       610        620 
TLRAGQAYDF VYVQGGPQAK VSVLSYN 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
Libertini E., Li Y., McQueen-Mason S.J.
J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC016041 Genomic DNA. Translation: AAF69707.1.
CP002684 Genomic DNA. Translation: AEE32368.1.
BT002935 mRNA. Translation: AAO22749.1.
BT005638 mRNA. Translation: AAO64058.1.
PIRB96527.
RefSeqNP_175323.1. NM_103786.2.
UniGeneAt.16674.

3D structure databases

ProteinModelPortalQ9M995.
SMRQ9M995. Positions 29-477.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM49. Carbohydrate-Binding Module Family 49.
GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbQ9M995.
PRIDEQ9M995.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G48930.1; AT1G48930.1; AT1G48930.
GeneID841315.
KEGGath:AT1G48930.

Organism-specific databases

TAIRAT1G48930.

Phylogenomic databases

eggNOGNOG05134.
HOGENOMHOG000021033.
InParanoidQ9M995.
OMAGHPITYL.
PhylomeDBQ9M995.

Enzyme and pathway databases

BioCycARA:AT1G48930-MONOMER.

Gene expression databases

ArrayExpressQ9M995.
GenevestigatorQ9M995.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR019028. CBM_49.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF09478. CBM49. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SMARTSM01063. CBM49. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN5_ARATH
AccessionPrimary (citable) accession number: Q9M995
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names