Q9M8Z8 (PLY8_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectate lyase 8
EC=4.2.2.2

Gene names

Ordered Locus Names:	At3g07010
ORF Names:	F17A9.16

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Cofactor	Binds 1 calcium ion. Required for its activity By similarity.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 416	392	Probable pectate lyase 8		PRO_0000024872
Sites
	Active site	294	1	Potential
	Metal binding	214	1	Calcium By similarity
	Metal binding	238	1	Calcium By similarity
	Metal binding	242	1	Calcium By similarity
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	52	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	17	1	A → T in AAM61584. Ref.3
	Sequence conflict	95	1	G → C in AAM61584. Ref.3
	Sequence conflict	382	1	A → S in AAM61584. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9M8Z8 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 27BBCB0FB6B047C2	FASTA	416	46,174
	10 20 30 40 50 60 MAVTKLILFA SALLLTALFI GVNASRSNET WHEHAVENPD EVAAMVDMSI RNSTERRRLG 70 80 90 100 110 120 YFSCATGNPI DDCWRCDRKW QLRRKRLADC SIGFGRNAIG GRDGRFYVVT DPGDDDPVNP 130 140 150 160 170 180 IPGTLRHAVI QDEPLWIIFK RDMVITLKQE LIMNSFKTID GRGVNVHIAN GACLTIQYVT 190 200 210 220 230 240 NIIVHGIHVH DCKPTGNAMV RSSPSHYGFR SMADGDAISI FGSSHIWIDH NSLSNCADGL 250 260 270 280 290 300 VDAVMSSTAI TVSNNFFTHH NEVMLLGHSD SYTRDKVMQV TIAYNHFGEG LIQRMPRCRH 310 320 330 340 350 360 GYFHVVNNDY THWEMYAIGG SAGPTINSQG NRFLAPVNPF AKEVTKREYT GESKWKHWNW 370 380 390 400 410 RSEGDLFLNG AFFTRSGAGA GANYARASSL SAKSSSLVGT MTSYSGALNC RAGRRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC016827 Genomic DNA. Translation: AAF27005.1. CP002686 Genomic DNA. Translation: AEE74488.1. AY085026 mRNA. Translation: AAM61584.1.
IPI	IPI00517964.
RefSeq	NP_187357.1. NM_111581.2.
UniGene	At.18756.
3D structure databases
ProteinModelPortal	Q9M8Z8.
SMR	Q9M8Z8. Positions 67-409.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9M8Z8.
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Proteomic databases
PRIDE	Q9M8Z8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G07010.1; AT3G07010.1; AT3G07010.
GeneID	819886.
GenomeReviews	Gene locus AT3G07010 in contig BA000014_GR.
KEGG	ath:AT3G07010.
NMPDR	fig|3702.1.peg.12722.
Organism-specific databases
TAIR	At3g07010.
Phylogenomic databases
GeneTree	EPGT00050000008990.
HOGENOM	HBG317381.
InParanoid	Q9M8Z8.
OMA	IANGACL.
PhylomeDB	Q9M8Z8.
ProtClustDB	CLSN2684811.
Gene expression databases
ArrayExpress	Q9M8Z8.
Genevestigator	Q9M8Z8.
GermOnline	AT3G07010. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR018082. AmbAllergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01728.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
PRINTS	PR00807. AMBALLERGEN.
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLY8_ARATH

Accession

Primary (citable) accession number: Q9M8Z8
Secondary accession number(s): Q8LF66

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	October 1, 2000
Last modified:	December 14, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program