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Q9M8Y0 (SEC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC
EC=2.4.1.-
Alternative name(s):
Protein SECRET AGENT
Gene names
Name:SEC
Ordered Locus Names:At3g04240
ORF Names:T6K12.14
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length977 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

O-linked N-acetylglucosamine transferase (OGT) that mediates O-glycosylation of capsid protein (CP) of virus in case of infection by Plum pox virus. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins. Its OGT activity has been proved in vitro but not in vivo. Required with SPY for gamete and seed development. Ref.5

Catalytic activity

UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide. Ref.1

Pathway

Protein modification; protein glycosylation.

Sequence similarities

Belongs to the O-GlcNAc transferase family.

Contains 13 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 977977Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SEC
PRO_0000191782

Regions

Repeat53 – 8634TPR 1
Repeat87 – 12034TPR 2
Repeat121 – 15434TPR 3
Repeat155 – 18834TPR 4
Repeat190 – 22233TPR 5
Repeat223 – 25634TPR 6
Repeat257 – 29034TPR 7
Repeat292 – 32433TPR 8
Repeat325 – 35834TPR 9
Repeat360 – 39233TPR 10
Repeat393 – 42634TPR 11
Repeat428 – 46033TPR 12
Repeat461 – 49434TPR 13
Region495 – 977483Catalytic region

Sequences

Sequence LengthMass (Da)Tools
Q9M8Y0 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 2AC617CB4B6D5409

FASTA977110,114
        10         20         30         40         50         60 
MISSKNGAAM ISRPVFLSDR VDEVFSRKLD LSVSSSSSSS LLQQFNKTHE GDDDARLALA 

        70         80         90        100        110        120 
HQLYKGGDFK QALEHSNMVY QRNPLRTDNL LLIGAIYYQL QEYDMCIARN EEALRIQPQF 

       130        140        150        160        170        180 
AECYGNMANA WKEKGDTDRA IRYYLIAIEL RPNFADAWSN LASAYMRKGR LSEATQCCQQ 

       190        200        210        220        230        240 
ALSLNPLLVD AHSNLGNLMK AQGLIHEAYS CYLEAVRIQP TFAIAWSNLA GLFMESGDLN 

       250        260        270        280        290        300 
RALQYYKEAV KLKPAFPDAY LNLGNVYKAL GRPTEAIMCY QHALQMRPNS AMAFGNIASI 

       310        320        330        340        350        360 
YYEQGQLDLA IRHYKQALSR DPRFLEAYNN LGNALKDIGR VDEAVRCYNQ CLALQPNHPQ 

       370        380        390        400        410        420 
AMANLGNIYM EWNMMGPASS LFKATLAVTT GLSAPFNNLA IIYKQQGNYS DAISCYNEVL 

       430        440        450        460        470        480 
RIDPLAADAL VNRGNTYKEI GRVTEAIQDY MHAINFRPTM AEAHANLASA YKDSGHVEAA 

       490        500        510        520        530        540 
ITSYKQALLL RPDFPEATCN LLHTLQCVCC WEDRSKMFAE VESIIRRQIN MSVLPSVQPF 

       550        560        570        580        590        600 
HAIAYPIDPI LALEISRKYA AHCSIIASRF GLPPFTHPAG LPVKREGGFK RLRIGYVSSD 

       610        620        630        640        650        660 
FGNHPLSHLM GSVFGMHNRE NVEVFCYALS ANDNTEWRQR IQSEAEHFLD VSAMSSDAIA 

       670        680        690        700        710        720 
KIINQDKIQI LINLNGYTKG ARNEIFAMQP APIQVSYMGF PGTTGATYID YLVTDEFVSP 

       730        740        750        760        770        780 
LQYAHIYSEK LVHLPHCYFV NDYKQKNQDV LDPNSKPKRS DYGLPEDKFI FACFNQLYKM 

       790        800        810        820        830        840 
DPEIVNTWCN ILKRVPNSAL WLLRFPAAGE MRFRTYAAAQ GVQPDQIIFT DVAMKSEHIR 

       850        860        870        880        890        900 
RSVLADVILD TPLCNGHTTG TDVLWAGVPM ITLPLEKMAT RVAGSLCLAT GLGHGMIVNS 

       910        920        930        940        950        960 
LEEYEEKAVS LALNKPKLQA LTKELRASRL TCPLFDTMRW VKNLERSYFK MWNLHCSGQQ 

       970 
PQHFKVLEND LEFPHDR

« Hide

References

« Hide 'large scale' references
[1]"Two O-linked N-acetylglucosamine transferase genes of Arabidopsis thaliana L. Heynh. have overlapping functions necessary for gamete and seed development."
Hartweck L.M., Scott C.L., Olszewski N.E.
Genetics 161:1279-1291(2002) [PubMed: 12136030] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Identification of secret agent as the O-GlcNAc transferase that participates in Plum pox virus infection."
Chen D., Juarez S., Hartweck L., Alamillo J.M., Simon-Mateo C., Perez J.J., Fernandez-Fernandez M.R., Olszewski N.E., Garcia J.A.
J. Virol. 79:9381-9387(2005) [PubMed: 16014901] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF441079 mRNA. Translation: AAL60196.1.
AC016829 Genomic DNA. Translation: AAF26789.1.
CP002686 Genomic DNA. Translation: AEE74056.1.
AY090938 mRNA. Translation: AAM13988.1.
AY117340 mRNA. Translation: AAM51415.1.
IPIIPI00524518.
RefSeqNP_187074.1. NM_111295.3.
UniGeneAt.27850.
At.71305.

3D structure databases

ProteinModelPortalQ9M8Y0.
SMRQ9M8Y0. Positions 47-965.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9M8Y0. 2 interactions.
STRINGQ9M8Y0.

Protein family/group databases

CAZyGT41. Glycosyltransferase Family 41.

Proteomic databases

PRIDEQ9M8Y0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G04240.1; AT3G04240.1; AT3G04240.
GeneID819579.
GenomeReviewsGene locus AT3G04240 in contig BA000014_GR.
KEGGath:AT3G04240.
NMPDRfig|3702.1.peg.12388.

Organism-specific databases

TAIRAt3g04240.

Phylogenomic databases

GeneTreeEPGT00050000009777.
HOGENOMHBG317910.
InParanoidQ9M8Y0.
OMANFPDAYC.
PhylomeDBQ9M8Y0.
ProtClustDBCLSN2684273.

Gene expression databases

ArrayExpressQ9M8Y0.
GenevestigatorQ9M8Y0.
GermOnlineAT3G04240. Arabidopsis thaliana.

Family and domain databases

InterProIPR001440. TPR-1.
IPR013026. TPR-contain.
IPR011990. TPR-like_helical.
IPR019734. TPR_repeat.
[Graphical view]
Gene3DG3DSA:1.25.40.10. TPR-like_helical. 2 hits.
KOK09667.
PfamPF00515. TPR_1. 9 hits.
[Graphical view]
SMARTSM00028. TPR. 11 hits.
[Graphical view]
PROSITEPS50005. TPR. 13 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSEC_ARATH
AccessionPrimary (citable) accession number: Q9M8Y0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: October 1, 2000
Last modified: December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents