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Protein

Potassium channel SKOR

Gene

SKOR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Highly selective outward-rectifying potassium channel. Involved in potassium release into the xylem sap toward the shoots. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by depolarization. The voltage-dependence of the channel is abolished by internal or external acidification. May interact with the cytoskeleton or with regulatory proteins.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi403 – 523cNMPAdd BLAST121

GO - Molecular functioni

  • outward rectifier potassium channel activity Source: TAIR

GO - Biological processi

  • potassium ion homeostasis Source: TAIR
  • potassium ion transport Source: TAIR
  • regulation of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

BioCyciARA:AT3G02850-MONOMER.
MetaCyc:MONOMER-14560.
ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Protein family/group databases

TCDBi1.A.1.4.5. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel SKOR
Alternative name(s):
Stelar K(+) outward rectifying channel
Gene namesi
Name:SKOR
Ordered Locus Names:At3g02850
ORF Names:F13E7.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G02850.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 86CytoplasmicSequence analysisAdd BLAST86
Transmembranei87 – 107Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini108 – 114ExtracellularSequence analysis7
Transmembranei115 – 135Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini136 – 158CytoplasmicSequence analysisAdd BLAST23
Transmembranei159 – 179Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini180 – 185ExtracellularSequence analysis6
Transmembranei186 – 206Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini207 – 220CytoplasmicSequence analysisAdd BLAST14
Transmembranei221 – 241Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini242 – 276ExtracellularSequence analysisAdd BLAST35
Intramembranei277 – 296Pore-forming; Name=Segment H5Sequence analysisAdd BLAST20
Topological domaini297 – 300ExtracellularSequence analysis4
Transmembranei301 – 321Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini322 – 828CytoplasmicSequence analysisAdd BLAST507

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541281 – 828Potassium channel SKORAdd BLAST828

Proteomic databases

PaxDbiQ9M8S6.
PRIDEiQ9M8S6.

Expressioni

Tissue specificityi

Expressed in root pericycle and xylem parenchyma, and in flower at a lower level.1 Publication

Inductioni

In roots, strongly inhibited by 2,4-dichlorophenoxyacetic acid (2,4-D), abscisic acid (ABA) and benzyladenine (BA) treatment or by potassium starvation.1 Publication

Gene expression databases

GenevisibleiQ9M8S6. AT.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.By similarity

Protein-protein interaction databases

BioGridi6385. 2 interactors.
IntActiQ9M8S6. 1 interactor.
STRINGi3702.AT3G02850.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M8S6.
SMRiQ9M8S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati545 – 576ANK 1Add BLAST32
Repeati580 – 609ANK 2Add BLAST30
Repeati613 – 642ANK 3Add BLAST30
Repeati644 – 673ANK 4Add BLAST30
Repeati677 – 706ANK 5Add BLAST30
Repeati710 – 740ANK 6Add BLAST31
Domaini756 – 828KHAPROSITE-ProRule annotationAdd BLAST73

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 KHA domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000240830.
InParanoidiQ9M8S6.
OMAiDPDNKWY.
OrthoDBiEOG093602RR.
PhylomeDBiQ9M8S6.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M8S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGSSGGGVS YRSGGESDVE LEDYEVDDFR DGIVESRGNR FNPLTNFLGL
60 70 80 90 100
DFAGGSGGKF TVINGIRDIS RGSIVHPDNR WYKAWTMFIL IWALYSSFFT
110 120 130 140 150
PLEFGFFRGL PENLFILDIA GQIAFLVDIV LTFFVAYRDS RTYRMIYKRS
160 170 180 190 200
SIALRYLKST FIIDLLACMP WDIIYKAAGE KEEVRYLLLI RLYRVHRVIL
210 220 230 240 250
FFHKMEKDIR INYLFTRIVK LIFVELYCTH TAACIFYYLA TTLPASQEGY
260 270 280 290 300
TWIGSLKLGD YSYSKFREID LWTRYTTSMY FAVVTMATVG YGDIHAVNMR
310 320 330 340 350
EMIFAMVYIS FDMILGAYLI GNMTALIVKG SKTERFRDKM ADIMRYMNRN
360 370 380 390 400
KLGRNIRGQI TGHLRLQYES SYTEAAVLQD IPVSIRAKIA QTLYLPYIEK
410 420 430 440 450
VPLFRGCSSE FINQIVIRLH EEFFLPGEVI MEQGSVVDQL YFVCHGVLEE
460 470 480 490 500
IGITKDGSEE IVAVLQPDHS FGEISILCNI PQPYTVRVAE LCRILRLDKQ
510 520 530 540 550
SFMNILEIFF HDGRRILNNL LEGKESNVRI KQLESDITFH ISKQEAELAL
560 570 580 590 600
KLNSAAFYGD LYQLKSLIRA GGDPNKTDYD GRSPLHLAAS RGYEDITLYL
610 620 630 640 650
IQESVDVNIK DKLGSTPLLE AIKNGNDRVA ALLVKEGATL NIENAGTFLC
660 670 680 690 700
TVVAKGDSDF LKRLLSNGID PNSKDYDHRT PLHVAASEGF YVLAIQLVEA
710 720 730 740 750
SANVLAKDRW GNTPLDEALG CGNKMLIKLL EDAKNSQISS FPSGSKEPKD
760 770 780 790 800
KVYKKKCTVY FSHPGDSKEK RRRGIVLWVP RSIEELIRTA KEQLNVPEAS
810 820
CVLSEDEAKI IDVDLISDGQ KLYLAVET
Length:828
Mass (Da):93,899
Last modified:October 1, 2000 - v1
Checksum:iC6C7B161C8904D03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16E → R in CAA11281 (PubMed:9741629).Curated1
Sequence conflicti547 – 548EL → DV in CAA11280 (PubMed:9741629).Curated2
Sequence conflicti547 – 548EL → DV in CAA11281 (PubMed:9741629).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223357 Genomic DNA. Translation: CAA11280.1.
AJ223358 mRNA. Translation: CAA11281.1.
AC018363 Genomic DNA. Translation: AAF26975.1.
CP002686 Genomic DNA. Translation: AEE73866.1.
PIRiT52046.
RefSeqiNP_186934.1. NM_111153.4.
UniGeneiAt.41071.
At.48613.

Genome annotation databases

EnsemblPlantsiAT3G02850.1; AT3G02850.1; AT3G02850.
GeneIDi821052.
GrameneiAT3G02850.1; AT3G02850.1; AT3G02850.
KEGGiath:AT3G02850.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223357 Genomic DNA. Translation: CAA11280.1.
AJ223358 mRNA. Translation: CAA11281.1.
AC018363 Genomic DNA. Translation: AAF26975.1.
CP002686 Genomic DNA. Translation: AEE73866.1.
PIRiT52046.
RefSeqiNP_186934.1. NM_111153.4.
UniGeneiAt.41071.
At.48613.

3D structure databases

ProteinModelPortaliQ9M8S6.
SMRiQ9M8S6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6385. 2 interactors.
IntActiQ9M8S6. 1 interactor.
STRINGi3702.AT3G02850.1.

Protein family/group databases

TCDBi1.A.1.4.5. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiQ9M8S6.
PRIDEiQ9M8S6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G02850.1; AT3G02850.1; AT3G02850.
GeneIDi821052.
GrameneiAT3G02850.1; AT3G02850.1; AT3G02850.
KEGGiath:AT3G02850.

Organism-specific databases

TAIRiAT3G02850.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000240830.
InParanoidiQ9M8S6.
OMAiDPDNKWY.
OrthoDBiEOG093602RR.
PhylomeDBiQ9M8S6.

Enzyme and pathway databases

BioCyciARA:AT3G02850-MONOMER.
MetaCyc:MONOMER-14560.
ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiQ9M8S6.

Gene expression databases

GenevisibleiQ9M8S6. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
PR01463. EAGCHANLFMLY.
SMARTiSM00248. ANK. 5 hits.
SM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKOR_ARATH
AccessioniPrimary (citable) accession number: Q9M8S6
Secondary accession number(s): O82630, Q9SB86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Loss-of-function mutation skor-1 leads to a reduced shoot potassium content.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.