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Protein

Potassium channel SKOR

Gene

SKOR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Highly selective outward-rectifying potassium channel. Involved in potassium release into the xylem sap toward the shoots. Assuming opened or closed conformations in response to the voltage difference across the membrane, the channel is activated by depolarization. The voltage-dependence of the channel is abolished by internal or external acidification. May interact with the cytoskeleton or with regulatory proteins.1 Publication

Miscellaneous

Loss-of-function mutation skor-1 leads to a reduced shoot potassium content.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi403 – 523cNMPAdd BLAST121

GO - Molecular functioni

  • outward rectifier potassium channel activity Source: TAIR

GO - Biological processi

  • potassium ion homeostasis Source: TAIR
  • potassium ion transport Source: TAIR
  • regulation of ion transmembrane transport Source: UniProtKB-KW
  • regulation of membrane potential Source: GO_Central

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Enzyme and pathway databases

BioCyciARA:AT3G02850-MONOMER
MetaCyc:MONOMER-14560

Protein family/group databases

TCDBi1.A.1.4.5 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel SKOR
Alternative name(s):
Stelar K(+) outward rectifying channel
Gene namesi
Name:SKOR
Ordered Locus Names:At3g02850
ORF Names:F13E7.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G02850
TAIRilocus:2075442 AT3G02850

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 86CytoplasmicSequence analysisAdd BLAST86
Transmembranei87 – 107Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini108 – 114ExtracellularSequence analysis7
Transmembranei115 – 135Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini136 – 158CytoplasmicSequence analysisAdd BLAST23
Transmembranei159 – 179Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini180 – 185ExtracellularSequence analysis6
Transmembranei186 – 206Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini207 – 220CytoplasmicSequence analysisAdd BLAST14
Transmembranei221 – 241Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini242 – 276ExtracellularSequence analysisAdd BLAST35
Intramembranei277 – 296Pore-forming; Name=Segment H5Sequence analysisAdd BLAST20
Topological domaini297 – 300ExtracellularSequence analysis4
Transmembranei301 – 321Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini322 – 828CytoplasmicSequence analysisAdd BLAST507

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541281 – 828Potassium channel SKORAdd BLAST828

Proteomic databases

PaxDbiQ9M8S6
PRIDEiQ9M8S6

Expressioni

Tissue specificityi

Expressed in root pericycle and xylem parenchyma, and in flower at a lower level.1 Publication

Inductioni

In roots, strongly inhibited by 2,4-dichlorophenoxyacetic acid (2,4-D), abscisic acid (ABA) and benzyladenine (BA) treatment or by potassium starvation.1 Publication

Gene expression databases

ExpressionAtlasiQ9M8S6 baseline and differential
GenevisibleiQ9M8S6 AT

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.By similarity

Protein-protein interaction databases

BioGridi63852 interactors.
IntActiQ9M8S6 1 interactor.
STRINGi3702.AT3G02850.1

Structurei

3D structure databases

ProteinModelPortaliQ9M8S6
SMRiQ9M8S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati545 – 576ANK 1Add BLAST32
Repeati580 – 609ANK 2Add BLAST30
Repeati613 – 642ANK 3Add BLAST30
Repeati644 – 673ANK 4Add BLAST30
Repeati677 – 706ANK 5Add BLAST30
Repeati710 – 740ANK 6Add BLAST31
Domaini756 – 828KHAPROSITE-ProRule annotationAdd BLAST73

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
HOGENOMiHOG000240830
InParanoidiQ9M8S6
KOiK21867
OMAiRDKMHPR
OrthoDBiEOG093602RR
PhylomeDBiQ9M8S6

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00038 CAP_ED, 1 hit
Gene3Di1.25.40.202 hits
2.60.120.101 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR005821 Ion_trans_dom
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR021789 KHA_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF11834 KHA, 1 hit
PRINTSiPR01415 ANKYRIN
PR01463 EAGCHANLFMLY
SMARTiView protein in SMART
SM00248 ANK, 5 hits
SM00100 cNMP, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits
PS50042 CNMP_BINDING_3, 1 hit
PS51490 KHA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9M8S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGSSGGGVS YRSGGESDVE LEDYEVDDFR DGIVESRGNR FNPLTNFLGL
60 70 80 90 100
DFAGGSGGKF TVINGIRDIS RGSIVHPDNR WYKAWTMFIL IWALYSSFFT
110 120 130 140 150
PLEFGFFRGL PENLFILDIA GQIAFLVDIV LTFFVAYRDS RTYRMIYKRS
160 170 180 190 200
SIALRYLKST FIIDLLACMP WDIIYKAAGE KEEVRYLLLI RLYRVHRVIL
210 220 230 240 250
FFHKMEKDIR INYLFTRIVK LIFVELYCTH TAACIFYYLA TTLPASQEGY
260 270 280 290 300
TWIGSLKLGD YSYSKFREID LWTRYTTSMY FAVVTMATVG YGDIHAVNMR
310 320 330 340 350
EMIFAMVYIS FDMILGAYLI GNMTALIVKG SKTERFRDKM ADIMRYMNRN
360 370 380 390 400
KLGRNIRGQI TGHLRLQYES SYTEAAVLQD IPVSIRAKIA QTLYLPYIEK
410 420 430 440 450
VPLFRGCSSE FINQIVIRLH EEFFLPGEVI MEQGSVVDQL YFVCHGVLEE
460 470 480 490 500
IGITKDGSEE IVAVLQPDHS FGEISILCNI PQPYTVRVAE LCRILRLDKQ
510 520 530 540 550
SFMNILEIFF HDGRRILNNL LEGKESNVRI KQLESDITFH ISKQEAELAL
560 570 580 590 600
KLNSAAFYGD LYQLKSLIRA GGDPNKTDYD GRSPLHLAAS RGYEDITLYL
610 620 630 640 650
IQESVDVNIK DKLGSTPLLE AIKNGNDRVA ALLVKEGATL NIENAGTFLC
660 670 680 690 700
TVVAKGDSDF LKRLLSNGID PNSKDYDHRT PLHVAASEGF YVLAIQLVEA
710 720 730 740 750
SANVLAKDRW GNTPLDEALG CGNKMLIKLL EDAKNSQISS FPSGSKEPKD
760 770 780 790 800
KVYKKKCTVY FSHPGDSKEK RRRGIVLWVP RSIEELIRTA KEQLNVPEAS
810 820
CVLSEDEAKI IDVDLISDGQ KLYLAVET
Length:828
Mass (Da):93,899
Last modified:October 1, 2000 - v1
Checksum:iC6C7B161C8904D03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16E → R in CAA11281 (PubMed:9741629).Curated1
Sequence conflicti547 – 548EL → DV in CAA11280 (PubMed:9741629).Curated2
Sequence conflicti547 – 548EL → DV in CAA11281 (PubMed:9741629).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223357 Genomic DNA Translation: CAA11280.1
AJ223358 mRNA Translation: CAA11281.1
AC018363 Genomic DNA Translation: AAF26975.1
CP002686 Genomic DNA Translation: AEE73866.1
PIRiT52046
RefSeqiNP_186934.1, NM_111153.4
UniGeneiAt.41071
At.48613

Genome annotation databases

EnsemblPlantsiAT3G02850.1; AT3G02850.1; AT3G02850
GeneIDi821052
GrameneiAT3G02850.1; AT3G02850.1; AT3G02850
KEGGiath:AT3G02850

Similar proteinsi

Entry informationi

Entry nameiSKOR_ARATH
AccessioniPrimary (citable) accession number: Q9M8S6
Secondary accession number(s): O82630, Q9SB86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome