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Q9M8R7 (P2C33_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable protein phosphatase 2C 33

Short name=AtPP2C33
EC=3.1.3.16
Alternative name(s):
AtPPC6;1
Gene names
Name:PPC6-1
Ordered Locus Names:At3g02750
ORF Names:F13E7.31
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Sequence caution

The sequence BX824075 differs from that shown. Reason: Sequencing errors.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9M8R7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9M8R7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     264-264: P → PGWIILCECMMLSCGCMMDPLIMFIGFFFIPSIELA
Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Probable protein phosphatase 2C 33
PRO_0000367960

Regions

Domain187 – 353167PP2C-like

Sites

Metal binding1001Manganese 1 By similarity
Metal binding1001Manganese 2 By similarity
Metal binding1011Manganese 1; via carbonyl oxygen By similarity
Metal binding3381Manganese 2 By similarity
Metal binding3841Manganese 2 By similarity

Natural variations

Alternative sequence2641P → PGWIILCECMMLSCGCMMDP LIMFIGFFFIPSIELA in isoform 2.
VSP_036766

Experimental info

Sequence conflict411F → L in AAL32574. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: D021BD5ACE735CF2

FASTA49253,715
        10         20         30         40         50         60 
MGSCLSAESR SPRPGSPCSP AFSVRKRKNS KKRPGSRNSS FDYRREEPLN QVPGRMFLNG 

        70         80         90        100        110        120 
STEVACIYTQ QGKKGPNQDA MVVWENFGSR TDTIFCGVFD GHGPYGHMVA KRVRDNLPLK 

       130        140        150        160        170        180 
LSAYWEAKVP VEGVLKAITT DTVNNVTNIN NPEDAAAAAA FVTAEEEPRT SADMEEENTE 

       190        200        210        220        230        240 
TQPELFQTLK ESFLKAFKVM DRELKFHGSV DCFCSGTTAV TLIKQGQYLV VGNVGDSRAV 

       250        260        270        280        290        300 
MGTRDSENTL VAVQLTVDLK PNLPAEAERI RKCRGRVFAL RDEPEVCRVW LPNCDSPGLA 

       310        320        330        340        350        360 
MARAFGDFCL KDFGLISVPD VSFRQLTEKD EFIVLATDGI WDVLSNEDVV AIVASAPSRS 

       370        380        390        400        410        420 
SAARALVESA VRAWRYKYPT SKVDDCAAVC LYLDSSNTNA ISTASSISKL EDGEEEELKA 

       430        440        450        460        470        480 
TTEDDDASGP SGLGRSSTVR SGKEIALDES ETEKLIKEAD NLDSEPGTEY SALEGVARVN 

       490 
TLLNLPRFVP GK 

« Hide

Isoform 2 [UniParc].

Checksum: E6296601474665D8
Show »

FASTA52757,674

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Arabidopsis ORF clones."
Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana."
Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-492.
[7]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC018363 Genomic DNA. Translation: AAF26985.1.
CP002686 Genomic DNA. Translation: AEE73854.1.
CP002686 Genomic DNA. Translation: AEE73855.1.
CP002686 Genomic DNA. Translation: AEE73856.1.
AY062496 mRNA. Translation: AAL32574.1.
BX824075 mRNA. No translation available.
BT020578 mRNA. Translation: AAW80851.1.
BT029026 mRNA. Translation: ABI93935.1.
AB079669 mRNA. Translation: BAB84698.1.
RefSeqNP_001030624.1. NM_001035547.1.
NP_001030625.1. NM_001035548.1.
NP_186924.1. NM_111143.3.
UniGeneAt.22072.

3D structure databases

ProteinModelPortalQ9M8R7.
SMRQ9M8R7. Positions 65-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid6243. 1 interaction.
IntActQ9M8R7. 1 interaction.
MINTMINT-8390499.

Proteomic databases

PaxDbQ9M8R7.
PRIDEQ9M8R7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G02750.1; AT3G02750.1; AT3G02750. [Q9M8R7-1]
AT3G02750.2; AT3G02750.2; AT3G02750. [Q9M8R7-1]
GeneID820909.
KEGGath:AT3G02750.

Organism-specific databases

TAIRAT3G02750.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000233897.
InParanoidQ9M8R7.
OMANFGSRTD.
PhylomeDBQ9M8R7.
ProtClustDBCLSN2685212.

Enzyme and pathway databases

BioCycARA:GQT-2832-MONOMER.
ARA:GQT-940-MONOMER.

Gene expression databases

GenevestigatorQ9M8R7.

Family and domain databases

Gene3D3.60.40.10. 2 hits.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 2 hits.
ProtoNetSearch...

Entry information

Entry nameP2C33_ARATH
AccessionPrimary (citable) accession number: Q9M8R7
Secondary accession number(s): Q2V3Z1, Q8SBC3, Q8W4L2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 1, 2000
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names