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Q9M8R7

- P2C33_ARATH

UniProt

Q9M8R7 - P2C33_ARATH

Protein

Probable protein phosphatase 2C 33

Gene

PPC6-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi100 – 1001Manganese 1By similarity
    Metal bindingi100 – 1001Manganese 2By similarity
    Metal bindingi101 – 1011Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi338 – 3381Manganese 2By similarity
    Metal bindingi384 – 3841Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:GQT-2832-MONOMER.
    ARA:GQT-940-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable protein phosphatase 2C 33 (EC:3.1.3.16)
    Short name:
    AtPP2C33
    Alternative name(s):
    AtPPC6;1
    Gene namesi
    Name:PPC6-1
    Ordered Locus Names:At3g02750
    ORF Names:F13E7.31
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G02750.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Probable protein phosphatase 2C 33PRO_0000367960Add
    BLAST

    Proteomic databases

    PaxDbiQ9M8R7.
    PRIDEiQ9M8R7.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9M8R7.

    Interactioni

    Protein-protein interaction databases

    BioGridi6243. 1 interaction.
    IntActiQ9M8R7. 1 interaction.
    MINTiMINT-8390499.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M8R7.
    SMRiQ9M8R7. Positions 71-352.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini187 – 353167PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000233897.
    InParanoidiQ9M8R7.
    OMAiNFGSRTD.
    PhylomeDBiQ9M8R7.

    Family and domain databases

    Gene3Di3.60.40.10. 2 hits.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 2 hits.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9M8R7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSCLSAESR SPRPGSPCSP AFSVRKRKNS KKRPGSRNSS FDYRREEPLN    50
    QVPGRMFLNG STEVACIYTQ QGKKGPNQDA MVVWENFGSR TDTIFCGVFD 100
    GHGPYGHMVA KRVRDNLPLK LSAYWEAKVP VEGVLKAITT DTVNNVTNIN 150
    NPEDAAAAAA FVTAEEEPRT SADMEEENTE TQPELFQTLK ESFLKAFKVM 200
    DRELKFHGSV DCFCSGTTAV TLIKQGQYLV VGNVGDSRAV MGTRDSENTL 250
    VAVQLTVDLK PNLPAEAERI RKCRGRVFAL RDEPEVCRVW LPNCDSPGLA 300
    MARAFGDFCL KDFGLISVPD VSFRQLTEKD EFIVLATDGI WDVLSNEDVV 350
    AIVASAPSRS SAARALVESA VRAWRYKYPT SKVDDCAAVC LYLDSSNTNA 400
    ISTASSISKL EDGEEEELKA TTEDDDASGP SGLGRSSTVR SGKEIALDES 450
    ETEKLIKEAD NLDSEPGTEY SALEGVARVN TLLNLPRFVP GK 492
    Length:492
    Mass (Da):53,715
    Last modified:October 1, 2000 - v1
    Checksum:iD021BD5ACE735CF2
    GO
    Isoform 2 (identifier: Q9M8R7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         264-264: P → PGWIILCECMMLSCGCMMDPLIMFIGFFFIPSIELA

    Note: May be due to an intron retention. No experimental confirmation available.

    Show »
    Length:527
    Mass (Da):57,674
    Checksum:iE6296601474665D8
    GO

    Sequence cautioni

    The sequence BX824075 differs from that shown. Reason: Sequencing errors.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti41 – 411F → L in AAL32574. (PubMed:14593172)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei264 – 2641P → PGWIILCECMMLSCGCMMDP LIMFIGFFFIPSIELA in isoform 2. CuratedVSP_036766

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018363 Genomic DNA. Translation: AAF26985.1.
    CP002686 Genomic DNA. Translation: AEE73854.1.
    CP002686 Genomic DNA. Translation: AEE73855.1.
    CP002686 Genomic DNA. Translation: AEE73856.1.
    AY062496 mRNA. Translation: AAL32574.1.
    BX824075 mRNA. No translation available.
    BT020578 mRNA. Translation: AAW80851.1.
    BT029026 mRNA. Translation: ABI93935.1.
    AB079669 mRNA. Translation: BAB84698.1.
    RefSeqiNP_001030624.1. NM_001035547.1. [Q9M8R7-1]
    NP_001030625.1. NM_001035548.1. [Q9M8R7-2]
    NP_186924.1. NM_111143.3. [Q9M8R7-1]
    UniGeneiAt.22072.

    Genome annotation databases

    EnsemblPlantsiAT3G02750.1; AT3G02750.1; AT3G02750. [Q9M8R7-1]
    AT3G02750.2; AT3G02750.2; AT3G02750. [Q9M8R7-1]
    GeneIDi820909.
    KEGGiath:AT3G02750.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018363 Genomic DNA. Translation: AAF26985.1 .
    CP002686 Genomic DNA. Translation: AEE73854.1 .
    CP002686 Genomic DNA. Translation: AEE73855.1 .
    CP002686 Genomic DNA. Translation: AEE73856.1 .
    AY062496 mRNA. Translation: AAL32574.1 .
    BX824075 mRNA. No translation available.
    BT020578 mRNA. Translation: AAW80851.1 .
    BT029026 mRNA. Translation: ABI93935.1 .
    AB079669 mRNA. Translation: BAB84698.1 .
    RefSeqi NP_001030624.1. NM_001035547.1. [Q9M8R7-1 ]
    NP_001030625.1. NM_001035548.1. [Q9M8R7-2 ]
    NP_186924.1. NM_111143.3. [Q9M8R7-1 ]
    UniGenei At.22072.

    3D structure databases

    ProteinModelPortali Q9M8R7.
    SMRi Q9M8R7. Positions 71-352.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 6243. 1 interaction.
    IntActi Q9M8R7. 1 interaction.
    MINTi MINT-8390499.

    Proteomic databases

    PaxDbi Q9M8R7.
    PRIDEi Q9M8R7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G02750.1 ; AT3G02750.1 ; AT3G02750 . [Q9M8R7-1 ]
    AT3G02750.2 ; AT3G02750.2 ; AT3G02750 . [Q9M8R7-1 ]
    GeneIDi 820909.
    KEGGi ath:AT3G02750.

    Organism-specific databases

    TAIRi AT3G02750.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000233897.
    InParanoidi Q9M8R7.
    OMAi NFGSRTD.
    PhylomeDBi Q9M8R7.

    Enzyme and pathway databases

    BioCyci ARA:GQT-2832-MONOMER.
    ARA:GQT-940-MONOMER.

    Gene expression databases

    Genevestigatori Q9M8R7.

    Family and domain databases

    Gene3Di 3.60.40.10. 2 hits.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis ORF clones."
      Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Substrate specificity of type 2C protein phosphatases (PP2C) in Arabidopsis thaliana."
      Izumi S., Yamada M., Ohsato H., Miyazaki S., Bohnert H.J., Fukuhara T.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-492.
    7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiP2C33_ARATH
    AccessioniPrimary (citable) accession number: Q9M8R7
    Secondary accession number(s): Q2V3Z1, Q8SBC3, Q8W4L2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3