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Q9M8K7

- DUS1B_ARATH

UniProt

Q9M8K7 - DUS1B_ARATH

Protein

Dual specificity protein phosphatase 1B

Gene

DSPTP1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Has a dual specificity toward Ser/Thr and Tyr-containing proteins. Prevents biotic and abiotic stress responses, including ozone, oxidative stress and pathogen attacks; represses MAPK activities during hypersensitive response to limit the spread of the HR response after infection by necrotrophic pathogen such as Botrytis cinerea. May be also involved in ABA and salt responses. Dephosphorylates MPK3 and MPK6.3 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei109 – 1091Phosphocysteine intermediateBy similarity

    GO - Molecular functioni

    1. MAP kinase phosphatase activity Source: TAIR
    2. protein binding Source: UniProtKB
    3. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
    4. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to oxidative stress Source: UniProtKB
    2. inactivation of MAPK activity Source: GOC
    3. modulation by symbiont of host defense-related programmed cell death Source: UniProtKB-KW
    4. negative regulation of MAP kinase activity Source: TAIR
    5. negative regulation of plant-type hypersensitive response Source: UniProtKB
    6. protein dephosphorylation Source: RefGenome
    7. response to oxidative stress Source: TAIR
    8. response to ozone Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Hypersensitive response elicitation

    Enzyme and pathway databases

    BioCyciARA:AT3G06110-MONOMER.
    ARA:GQT-438-MONOMER.
    ReactomeiREACT_208246. ERKs are inactivated.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual specificity protein phosphatase 1B (EC:3.1.3.16, EC:3.1.3.48)
    Short name:
    AtDsPTP1B
    Alternative name(s):
    MAPK phosphatase 2
    Short name:
    AtMKP2
    Gene namesi
    Name:DSPTP1B
    Synonyms:MKP2
    Ordered Locus Names:At3g06110
    ORF Names:F28L1.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G06110.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: Upon fungal elicitation, relocalizes surrounding spherical structures that could correspond to epiplasts.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: TAIR
    3. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Delayed wilting symptoms in response to Ralstonia solanacearum and, by contrast, acceleration of disease progression during Botrytis cinerea infection, suggesting that this phosphatase plays differential functions in biotrophic versus necrotrophic pathogen-induced responses. Prolonged MPK3 and MPK6 activation during ozone treatment.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi109 – 1091C → S: Loss of phosphatase activity. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 167167Dual specificity protein phosphatase 1BPRO_0000415897Add
    BLAST

    Proteomic databases

    PRIDEiQ9M8K7.

    Expressioni

    Tissue specificityi

    Expressed in flowers, seedlings, roots, leaves, and seeds. Present in stomata and meristematic cells.1 Publication

    Developmental stagei

    In flowers, expressed in stigmatic papillae, anthers, pollen grains and floral abscission zones. As flowers mature, progressively restricted to the abscission zones and the septum of the siliques. During seed development Mainly detected in seeds endosperm layer until the fourth day after germination. In young seedlings, confined to the cotyledons. Later observed in roots, especially in vascular organs and at branching points of lateral roots. In adult leaves, particularly localized in vascular tissues and hydathodes; mostly present in both young and senescent leaves, tissues undergoing developmental transitions.1 Publication

    Inductioni

    Accumulates in response to stress conditions caused by abscisic acid (ABA) or salt treatment.1 Publication

    Gene expression databases

    GenevestigatoriQ9M8K7.

    Interactioni

    Subunit structurei

    Associates with MPK3 and MPK6. Interacts with MPK6 is promoted during HR-like responses triggered by fungal elicitors, whereas interaction with MPK3 in repressed.2 Publications

    Protein-protein interaction databases

    STRINGi3702.AT3G06110.2-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M8K7.
    SMRiQ9M8K7. Positions 22-161.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini92 – 15665Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000233767.
    InParanoidiQ9M8K7.
    OMAiRSKRPQV.
    PhylomeDBiQ9M8K7.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9M8K7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEKVVDLFGV GEANSQKLLE GGKDLSEIQQ GLFIGSVAEA NNKDFLKSSN    50
    ITHVLTVAVA LAPPYPDDFV YKVIEVVDRS ETDLTVYFDE CYSFIDQAIQ 100
    SGGGVLVHCF MGMSRSVTIV VAYLMKKHGM GFSKAMELVR SRRHQAYPNP 150
    GFISQLQQFE KSIQGNA 167
    Length:167
    Mass (Da):18,431
    Last modified:October 1, 2000 - v1
    Checksum:i57D722910B79A900
    GO
    Isoform 2 (identifier: Q9M8K7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         77-86: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:157
    Mass (Da):17,315
    Checksum:i7468282915C904E1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei77 – 8610Missing in isoform 2. 1 PublicationVSP_042414

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018907 Genomic DNA. Translation: AAF30304.1.
    CP002686 Genomic DNA. Translation: AEE74344.1.
    CP002686 Genomic DNA. Translation: AEE74345.1.
    CP002686 Genomic DNA. Translation: AEE74346.1.
    AK117443 mRNA. Translation: BAC42108.1.
    BT005135 mRNA. Translation: AAO50668.1.
    AY085765 mRNA. Translation: AAM62982.1.
    RefSeqiNP_001189821.1. NM_001202892.1. [Q9M8K7-1]
    NP_566272.1. NM_111486.3. [Q9M8K7-2]
    NP_850522.1. NM_180191.2. [Q9M8K7-1]
    UniGeneiAt.40567.

    Genome annotation databases

    EnsemblPlantsiAT3G06110.2; AT3G06110.2; AT3G06110. [Q9M8K7-1]
    AT3G06110.3; AT3G06110.3; AT3G06110. [Q9M8K7-1]
    GeneIDi819784.
    KEGGiath:AT3G06110.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC018907 Genomic DNA. Translation: AAF30304.1 .
    CP002686 Genomic DNA. Translation: AEE74344.1 .
    CP002686 Genomic DNA. Translation: AEE74345.1 .
    CP002686 Genomic DNA. Translation: AEE74346.1 .
    AK117443 mRNA. Translation: BAC42108.1 .
    BT005135 mRNA. Translation: AAO50668.1 .
    AY085765 mRNA. Translation: AAM62982.1 .
    RefSeqi NP_001189821.1. NM_001202892.1. [Q9M8K7-1 ]
    NP_566272.1. NM_111486.3. [Q9M8K7-2 ]
    NP_850522.1. NM_180191.2. [Q9M8K7-1 ]
    UniGenei At.40567.

    3D structure databases

    ProteinModelPortali Q9M8K7.
    SMRi Q9M8K7. Positions 22-161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G06110.2-P.

    Proteomic databases

    PRIDEi Q9M8K7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G06110.2 ; AT3G06110.2 ; AT3G06110 . [Q9M8K7-1 ]
    AT3G06110.3 ; AT3G06110.3 ; AT3G06110 . [Q9M8K7-1 ]
    GeneIDi 819784.
    KEGGi ath:AT3G06110.

    Organism-specific databases

    TAIRi AT3G06110.

    Phylogenomic databases

    HOGENOMi HOG000233767.
    InParanoidi Q9M8K7.
    OMAi RSKRPQV.
    PhylomeDBi Q9M8K7.

    Enzyme and pathway databases

    BioCyci ARA:AT3G06110-MONOMER.
    ARA:GQT-438-MONOMER.
    Reactomei REACT_208246. ERKs are inactivated.

    Miscellaneous databases

    PROi Q9M8K7.

    Gene expression databases

    Genevestigatori Q9M8K7.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    [Graphical view ]
    SMARTi SM00195. DSPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. "Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs."
      Lee J.S., Ellis B.E.
      J. Biol. Chem. 282:25020-25029(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS PHOSPHATASE, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-109.
      Strain: cv. Columbia.
    7. "MAPK phosphatase MKP2 mediates disease responses in Arabidopsis and functionally interacts with MPK3 and MPK6."
      Lumbreras V., Vilela B., Irar S., Sole M., Capellades M., Valls M., Coca M., Pages M.
      Plant J. 63:1017-1030(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEFENSE RESPONSE, INTERACTION WITH MPK3 AND MPK6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    8. "Regulation of MAPK signaling and cell death by MAPK phosphatase MKP2."
      Vilela B., Pages M., Lumbreras V.
      Plant Signal. Behav. 5:1497-1500(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MPK6, MUTAGENESIS OF CYS-109, INDUCTION BY STRESS CONDITIONS.

    Entry informationi

    Entry nameiDUS1B_ARATH
    AccessioniPrimary (citable) accession number: Q9M8K7
    Secondary accession number(s): Q8LDW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2012
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3