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Protein

Dual specificity protein phosphatase 1B

Gene

DSPTP1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a dual specificity toward Ser/Thr and Tyr-containing proteins. Prevents biotic and abiotic stress responses, including ozone, oxidative stress and pathogen attacks; represses MAPK activities during hypersensitive response to limit the spread of the HR response after infection by necrotrophic pathogen such as Botrytis cinerea. May be also involved in ABA and salt responses. Dephosphorylates MPK3 and MPK6.3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei109Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxidative stress Source: UniProtKB
  • intracellular signal transduction Source: TAIR
  • modulation by symbiont of host defense-related programmed cell death Source: UniProtKB-KW
  • negative regulation of MAP kinase activity Source: TAIR
  • negative regulation of plant-type hypersensitive response Source: UniProtKB
  • response to oxidative stress Source: TAIR
  • response to ozone Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Hypersensitive response elicitation

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 1B (EC:3.1.3.16, EC:3.1.3.48)
Short name:
AtDsPTP1B
Alternative name(s):
MAPK phosphatase 2
Short name:
AtMKP2
Gene namesi
Name:DSPTP1B
Synonyms:MKP2
Ordered Locus Names:At3g06110
ORF Names:F28L1.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G06110.

Subcellular locationi

  • Nucleus
  • Cytoplasm

  • Note: Upon fungal elicitation, relocalizes surrounding spherical structures that could correspond to epiplasts.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed wilting symptoms in response to Ralstonia solanacearum and, by contrast, acceleration of disease progression during Botrytis cinerea infection, suggesting that this phosphatase plays differential functions in biotrophic versus necrotrophic pathogen-induced responses. Prolonged MPK3 and MPK6 activation during ozone treatment.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109C → S: Loss of phosphatase activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004158971 – 167Dual specificity protein phosphatase 1BAdd BLAST167

Proteomic databases

PaxDbiQ9M8K7.

PTM databases

iPTMnetiQ9M8K7.

Expressioni

Tissue specificityi

Expressed in flowers, seedlings, roots, leaves, and seeds. Present in stomata and meristematic cells.1 Publication

Developmental stagei

In flowers, expressed in stigmatic papillae, anthers, pollen grains and floral abscission zones. As flowers mature, progressively restricted to the abscission zones and the septum of the siliques. During seed development Mainly detected in seeds endosperm layer until the fourth day after germination. In young seedlings, confined to the cotyledons. Later observed in roots, especially in vascular organs and at branching points of lateral roots. In adult leaves, particularly localized in vascular tissues and hydathodes; mostly present in both young and senescent leaves, tissues undergoing developmental transitions.1 Publication

Inductioni

Accumulates in response to stress conditions caused by abscisic acid (ABA) or salt treatment.1 Publication

Gene expression databases

GenevisibleiQ9M8K7. AT.

Interactioni

Subunit structurei

Associates with MPK3 and MPK6. Interacts with MPK6 is promoted during HR-like responses triggered by fungal elicitors, whereas interaction with MPK3 in repressed.2 Publications

Protein-protein interaction databases

BioGridi5119. 2 interactors.
STRINGi3702.AT3G06110.2.

Structurei

3D structure databases

ProteinModelPortaliQ9M8K7.
SMRiQ9M8K7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini92 – 156Tyrosine-protein phosphataseAdd BLAST65

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
HOGENOMiHOG000233767.
InParanoidiQ9M8K7.
KOiK20551.
OMAiTVYFDEC.
OrthoDBiEOG09360N3Q.
PhylomeDBiQ9M8K7.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9M8K7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKVVDLFGV GEANSQKLLE GGKDLSEIQQ GLFIGSVAEA NNKDFLKSSN
60 70 80 90 100
ITHVLTVAVA LAPPYPDDFV YKVIEVVDRS ETDLTVYFDE CYSFIDQAIQ
110 120 130 140 150
SGGGVLVHCF MGMSRSVTIV VAYLMKKHGM GFSKAMELVR SRRHQAYPNP
160
GFISQLQQFE KSIQGNA
Length:167
Mass (Da):18,431
Last modified:October 1, 2000 - v1
Checksum:i57D722910B79A900
GO
Isoform 2 (identifier: Q9M8K7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-86: Missing.

Note: No experimental confirmation available.
Show »
Length:157
Mass (Da):17,315
Checksum:i7468282915C904E1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04241477 – 86Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018907 Genomic DNA. Translation: AAF30304.1.
CP002686 Genomic DNA. Translation: AEE74344.1.
CP002686 Genomic DNA. Translation: AEE74345.1.
CP002686 Genomic DNA. Translation: AEE74346.1.
AK117443 mRNA. Translation: BAC42108.1.
BT005135 mRNA. Translation: AAO50668.1.
AY085765 mRNA. Translation: AAM62982.1.
RefSeqiNP_001189821.1. NM_001202892.1. [Q9M8K7-1]
NP_566272.1. NM_111486.3. [Q9M8K7-2]
NP_850522.1. NM_180191.3. [Q9M8K7-1]
UniGeneiAt.40567.

Genome annotation databases

EnsemblPlantsiAT3G06110.2; AT3G06110.2; AT3G06110. [Q9M8K7-1]
AT3G06110.3; AT3G06110.3; AT3G06110. [Q9M8K7-1]
GeneIDi819784.
GrameneiAT3G06110.2; AT3G06110.2; AT3G06110.
AT3G06110.3; AT3G06110.3; AT3G06110.
KEGGiath:AT3G06110.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018907 Genomic DNA. Translation: AAF30304.1.
CP002686 Genomic DNA. Translation: AEE74344.1.
CP002686 Genomic DNA. Translation: AEE74345.1.
CP002686 Genomic DNA. Translation: AEE74346.1.
AK117443 mRNA. Translation: BAC42108.1.
BT005135 mRNA. Translation: AAO50668.1.
AY085765 mRNA. Translation: AAM62982.1.
RefSeqiNP_001189821.1. NM_001202892.1. [Q9M8K7-1]
NP_566272.1. NM_111486.3. [Q9M8K7-2]
NP_850522.1. NM_180191.3. [Q9M8K7-1]
UniGeneiAt.40567.

3D structure databases

ProteinModelPortaliQ9M8K7.
SMRiQ9M8K7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5119. 2 interactors.
STRINGi3702.AT3G06110.2.

PTM databases

iPTMnetiQ9M8K7.

Proteomic databases

PaxDbiQ9M8K7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G06110.2; AT3G06110.2; AT3G06110. [Q9M8K7-1]
AT3G06110.3; AT3G06110.3; AT3G06110. [Q9M8K7-1]
GeneIDi819784.
GrameneiAT3G06110.2; AT3G06110.2; AT3G06110.
AT3G06110.3; AT3G06110.3; AT3G06110.
KEGGiath:AT3G06110.

Organism-specific databases

TAIRiAT3G06110.

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
HOGENOMiHOG000233767.
InParanoidiQ9M8K7.
KOiK20551.
OMAiTVYFDEC.
OrthoDBiEOG09360N3Q.
PhylomeDBiQ9M8K7.

Miscellaneous databases

PROiQ9M8K7.

Gene expression databases

GenevisibleiQ9M8K7. AT.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS1B_ARATH
AccessioniPrimary (citable) accession number: Q9M8K7
Secondary accession number(s): Q8LDW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.