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Q9M8K7

- DUS1B_ARATH

UniProt

Q9M8K7 - DUS1B_ARATH

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Protein

Dual specificity protein phosphatase 1B

Gene

DSPTP1B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a dual specificity toward Ser/Thr and Tyr-containing proteins. Prevents biotic and abiotic stress responses, including ozone, oxidative stress and pathogen attacks; represses MAPK activities during hypersensitive response to limit the spread of the HR response after infection by necrotrophic pathogen such as Botrytis cinerea. May be also involved in ABA and salt responses. Dephosphorylates MPK3 and MPK6.3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Phosphocysteine intermediateBy similarity

GO - Molecular functioni

  1. MAP kinase phosphatase activity Source: TAIR
  2. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
  3. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular response to oxidative stress Source: UniProtKB
  2. inactivation of MAPK activity Source: GOC
  3. intracellular signal transduction Source: TAIR
  4. modulation by symbiont of host defense-related programmed cell death Source: UniProtKB-KW
  5. negative regulation of MAP kinase activity Source: TAIR
  6. negative regulation of plant-type hypersensitive response Source: UniProtKB
  7. protein dephosphorylation Source: RefGenome
  8. response to oxidative stress Source: TAIR
  9. response to ozone Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Hypersensitive response elicitation

Enzyme and pathway databases

BioCyciARA:AT3G06110-MONOMER.
ARA:GQT-438-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 1B (EC:3.1.3.16, EC:3.1.3.48)
Short name:
AtDsPTP1B
Alternative name(s):
MAPK phosphatase 2
Short name:
AtMKP2
Gene namesi
Name:DSPTP1B
Synonyms:MKP2
Ordered Locus Names:At3g06110
ORF Names:F28L1.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G06110.

Subcellular locationi

Nucleus. Cytoplasm
Note: Upon fungal elicitation, relocalizes surrounding spherical structures that could correspond to epiplasts.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: TAIR
  3. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed wilting symptoms in response to Ralstonia solanacearum and, by contrast, acceleration of disease progression during Botrytis cinerea infection, suggesting that this phosphatase plays differential functions in biotrophic versus necrotrophic pathogen-induced responses. Prolonged MPK3 and MPK6 activation during ozone treatment.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi109 – 1091C → S: Loss of phosphatase activity. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 167167Dual specificity protein phosphatase 1BPRO_0000415897Add
BLAST

Proteomic databases

PRIDEiQ9M8K7.

Expressioni

Tissue specificityi

Expressed in flowers, seedlings, roots, leaves, and seeds. Present in stomata and meristematic cells.1 Publication

Developmental stagei

In flowers, expressed in stigmatic papillae, anthers, pollen grains and floral abscission zones. As flowers mature, progressively restricted to the abscission zones and the septum of the siliques. During seed development Mainly detected in seeds endosperm layer until the fourth day after germination. In young seedlings, confined to the cotyledons. Later observed in roots, especially in vascular organs and at branching points of lateral roots. In adult leaves, particularly localized in vascular tissues and hydathodes; mostly present in both young and senescent leaves, tissues undergoing developmental transitions.1 Publication

Inductioni

Accumulates in response to stress conditions caused by abscisic acid (ABA) or salt treatment.1 Publication

Gene expression databases

GenevestigatoriQ9M8K7.

Interactioni

Subunit structurei

Associates with MPK3 and MPK6. Interacts with MPK6 is promoted during HR-like responses triggered by fungal elicitors, whereas interaction with MPK3 in repressed.2 Publications

Protein-protein interaction databases

STRINGi3702.AT3G06110.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ9M8K7.
SMRiQ9M8K7. Positions 22-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini92 – 15665Tyrosine-protein phosphataseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000233767.
InParanoidiQ9M8K7.
OMAiRSKRPQV.
PhylomeDBiQ9M8K7.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9M8K7-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKVVDLFGV GEANSQKLLE GGKDLSEIQQ GLFIGSVAEA NNKDFLKSSN
60 70 80 90 100
ITHVLTVAVA LAPPYPDDFV YKVIEVVDRS ETDLTVYFDE CYSFIDQAIQ
110 120 130 140 150
SGGGVLVHCF MGMSRSVTIV VAYLMKKHGM GFSKAMELVR SRRHQAYPNP
160
GFISQLQQFE KSIQGNA
Length:167
Mass (Da):18,431
Last modified:October 1, 2000 - v1
Checksum:i57D722910B79A900
GO
Isoform 2 (identifier: Q9M8K7-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-86: Missing.

Note: No experimental confirmation available.

Show »
Length:157
Mass (Da):17,315
Checksum:i7468282915C904E1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei77 – 8610Missing in isoform 2. 1 PublicationVSP_042414

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018907 Genomic DNA. Translation: AAF30304.1.
CP002686 Genomic DNA. Translation: AEE74344.1.
CP002686 Genomic DNA. Translation: AEE74345.1.
CP002686 Genomic DNA. Translation: AEE74346.1.
AK117443 mRNA. Translation: BAC42108.1.
BT005135 mRNA. Translation: AAO50668.1.
AY085765 mRNA. Translation: AAM62982.1.
RefSeqiNP_001189821.1. NM_001202892.1. [Q9M8K7-1]
NP_566272.1. NM_111486.3. [Q9M8K7-2]
NP_850522.1. NM_180191.2. [Q9M8K7-1]
UniGeneiAt.40567.

Genome annotation databases

EnsemblPlantsiAT3G06110.2; AT3G06110.2; AT3G06110. [Q9M8K7-1]
AT3G06110.3; AT3G06110.3; AT3G06110. [Q9M8K7-1]
GeneIDi819784.
KEGGiath:AT3G06110.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC018907 Genomic DNA. Translation: AAF30304.1 .
CP002686 Genomic DNA. Translation: AEE74344.1 .
CP002686 Genomic DNA. Translation: AEE74345.1 .
CP002686 Genomic DNA. Translation: AEE74346.1 .
AK117443 mRNA. Translation: BAC42108.1 .
BT005135 mRNA. Translation: AAO50668.1 .
AY085765 mRNA. Translation: AAM62982.1 .
RefSeqi NP_001189821.1. NM_001202892.1. [Q9M8K7-1 ]
NP_566272.1. NM_111486.3. [Q9M8K7-2 ]
NP_850522.1. NM_180191.2. [Q9M8K7-1 ]
UniGenei At.40567.

3D structure databases

ProteinModelPortali Q9M8K7.
SMRi Q9M8K7. Positions 22-161.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G06110.2-P.

Proteomic databases

PRIDEi Q9M8K7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G06110.2 ; AT3G06110.2 ; AT3G06110 . [Q9M8K7-1 ]
AT3G06110.3 ; AT3G06110.3 ; AT3G06110 . [Q9M8K7-1 ]
GeneIDi 819784.
KEGGi ath:AT3G06110.

Organism-specific databases

TAIRi AT3G06110.

Phylogenomic databases

HOGENOMi HOG000233767.
InParanoidi Q9M8K7.
OMAi RSKRPQV.
PhylomeDBi Q9M8K7.

Enzyme and pathway databases

BioCyci ARA:AT3G06110-MONOMER.
ARA:GQT-438-MONOMER.

Miscellaneous databases

PROi Q9M8K7.

Gene expression databases

Genevestigatori Q9M8K7.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
[Graphical view ]
PANTHERi PTHR10159. PTHR10159. 1 hit.
Pfami PF00782. DSPc. 1 hit.
[Graphical view ]
SMARTi SM00195. DSPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs."
    Lee J.S., Ellis B.E.
    J. Biol. Chem. 282:25020-25029(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PHOSPHATASE, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-109.
    Strain: cv. Columbia.
  7. "MAPK phosphatase MKP2 mediates disease responses in Arabidopsis and functionally interacts with MPK3 and MPK6."
    Lumbreras V., Vilela B., Irar S., Sole M., Capellades M., Valls M., Coca M., Pages M.
    Plant J. 63:1017-1030(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEFENSE RESPONSE, INTERACTION WITH MPK3 AND MPK6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia.
  8. "Regulation of MAPK signaling and cell death by MAPK phosphatase MKP2."
    Vilela B., Pages M., Lumbreras V.
    Plant Signal. Behav. 5:1497-1500(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MPK6, MUTAGENESIS OF CYS-109, INDUCTION BY STRESS CONDITIONS.

Entry informationi

Entry nameiDUS1B_ARATH
AccessioniPrimary (citable) accession number: Q9M8K7
Secondary accession number(s): Q8LDW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3