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Protein

Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial

Gene

At1g74260

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential to the male gametophyte development. Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.1 Publication

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial (At1g74260)
  2. Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic (PUR5)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei786 – 7861ATP; via carbonyl oxygenBy similarity
Metal bindingi787 – 7871MagnesiumBy similarity
Metal bindingi826 – 8261MagnesiumBy similarity
Metal bindingi830 – 8301MagnesiumBy similarity
Metal bindingi989 – 9891MagnesiumBy similarity
Binding sitei991 – 9911ATPBy similarity
Active sitei1235 – 12351NucleophileBy similarity
Active sitei1366 – 13661By similarity
Active sitei1368 – 13681By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi407 – 41812ATPSequence analysisAdd
BLAST
Nucleotide bindingi487 – 4893ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G74260-MONOMER.
ReactomeiR-ATH-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial (EC:6.3.5.3)
Short name:
FGAM synthase
Short name:
FGAMS
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase
Short name:
FGAR amidotransferase
Short name:
FGAR-AT
Formylglycinamide ribotide amidotransferase
Gene namesi
Ordered Locus Names:At1g74260
ORF Names:F1O17.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G74260.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal to the male gametophyte.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5353Chloroplast and mitochondrionSequence analysisAdd
BLAST
Chaini54 – 14071354Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrialPRO_0000029880Add
BLAST

Proteomic databases

PaxDbiQ9M8D3.
PRIDEiQ9M8D3.

Expressioni

Gene expression databases

GenevisibleiQ9M8D3. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G74260.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M8D3.
SMRiQ9M8D3. Positions 287-676, 1138-1369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1141 – 1381241Glutamine amidotransferase type-1Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.Curated

Keywords - Domaini

Glutamine amidotransferase, Transit peptide

Phylogenomic databases

eggNOGiENOG410JUJJ. Eukaryota.
KOG1907. Eukaryota.
COG0046. LUCA.
COG0047. LUCA.
HOGENOMiHOG000261358.
InParanoidiQ9M8D3.
KOiK01952.
OMAiLSANWMW.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M8D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSQATRAA LFLNGSNRQA MLLQRSSMSQ LWGSVRMRTS RLSLNRTKAV
60 70 80 90 100
SLRCSAQPNK PKAAVSTGSF VTADELPSLV EKPAAEVIHF YRVPLIQESA
110 120 130 140 150
NAELLKAVQT KISNQIVSLT TEQSFNIGLE SKLKDEKLSV LKWILQETYE
160 170 180 190 200
PENLGTDSFL ERKKQEGLHA VIVEVGPRLS FTTAWSTNAV SICRACGLDE
210 220 230 240 250
VTRLERSRRY LLFSKEPLLE NQIKEFAAMV HDRMTECVYT QKLVSFETNV
260 270 280 290 300
VPEEVKYVPV MEKGRKALEE INQEMGLAFD EQDLQYYTRL FREDIKRDPT
310 320 330 340 350
NVELFDIAQS NSEHSRHWFF AGNMVIDGKP MDKSLMQIVK STWEANRNNS
360 370 380 390 400
VIGFKDNSSA IRGFLVNQLR PLLPGSVCLL DVSARDLDIL FTAETHNFPC
410 420 430 440 450
AVAPYPGAET GAGGRIRDTH ATGRGSFVVA STSGYCVGNL NMEGSYAPWE
460 470 480 490 500
DSSFQYPSNL ASPLQILIDA SNGASDYGNK FGEPMIQGYT RTFGMRLPSG
510 520 530 540 550
DRREWLKPIM FSAGIGQIDH THITKGEPEV GMLVVKIGGP AYRIGMGGGA
560 570 580 590 600
ASSMVSGQND AELDFNAVQR GDAEMSQKLY RVVRACIEMG EKNPIISIHD
610 620 630 640 650
QGAGGNCNVV KEIIYPQGAE IDIRAVVVGD HTMSVLEIWG AEYQEQDAIL
660 670 680 690 700
VKAESREILQ SICKRERLSM AVIGTINGGG RCTLIDSTAA AKCSKEGLPP
710 720 730 740 750
PPPAVDLELE KVLGDMPKKT FKFNRIAYAR EPLDIAPGIT LMDALKRVLR
760 770 780 790 800
LPSVSSKRFL TTKVDRCVTG LVAQQQTVGP LQITLADVAV IAQTFTDLTG
810 820 830 840 850
GACAIGEQPI KGLLDPKAMA RLAVGEALTN LVWAKVTALS DVKASGNWMY
860 870 880 890 900
AAKLEGEGSA MYDAAIALSE AMIELGIAID GGKDSLSMAA HADGEVVKAP
910 920 930 940 950
GNLVISAYVT CPDITKTVTP DLKLGGDDGI LLHVDLAKGK RRLGGSALAQ
960 970 980 990 1000
VFGQIGNDCP DLDDVPYLKN VFDGVQALIA ENLVSAGHDI SDGGLVVTAL
1010 1020 1030 1040 1050
EMAFAGNKGI NLDLASNGIS LFETLFSEEL GLVLEISKTN LDAVMEKLRA
1060 1070 1080 1090 1100
FDVTAEIIGN VTDSPLIEVK VDGITHLSEK TSFLRDMWED TSFQLEKLQR
1110 1120 1130 1140 1150
LASCVEMEKE GLKFRHEPNW KLSFIPSSTN NNYMSQDVKP KVAVIREEGS
1160 1170 1180 1190 1200
NGDREMSAAF YAAGFEPWDV TVSDLLAGDI TLDQFRGIVF VGGFSYADVL
1210 1220 1230 1240 1250
DSAKGWAASI RFNEPVLSQF QEFYKRPDTF SLGICNGCQL MALLGWVPGP
1260 1270 1280 1290 1300
QVGGSLDTSQ PRFVHNESGR FECRFTSVTI KDSPSIMLKG MEGSTLGVWA
1310 1320 1330 1340 1350
AHGEGRAYFP DEGVLDHMLH SDLAPLRYCD DDGNVTEAYP FNLNGSPLGI
1360 1370 1380 1390 1400
AAICSPDGRH LAMMPHPERC FLMWQFPWYP TSWDVEKAGP SPWLKMFQNA

RDWLESC
Length:1,407
Mass (Da):153,954
Last modified:June 13, 2012 - v3
Checksum:i49F270F0E2CEBDCB
GO

Sequence cautioni

The sequence AAG52403.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence ABW87767.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU091297 mRNA. Translation: ABW87767.1. Different initiation.
AC020579 Genomic DNA. Translation: AAG52403.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35571.1.
PIRiA96771.
RefSeqiNP_177566.3. NM_106086.4.
UniGeneiAt.34898.

Genome annotation databases

EnsemblPlantsiAT1G74260.1; AT1G74260.1; AT1G74260.
GeneIDi843766.
GrameneiAT1G74260.1; AT1G74260.1; AT1G74260.
KEGGiath:AT1G74260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU091297 mRNA. Translation: ABW87767.1. Different initiation.
AC020579 Genomic DNA. Translation: AAG52403.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35571.1.
PIRiA96771.
RefSeqiNP_177566.3. NM_106086.4.
UniGeneiAt.34898.

3D structure databases

ProteinModelPortaliQ9M8D3.
SMRiQ9M8D3. Positions 287-676, 1138-1369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G74260.1.

Proteomic databases

PaxDbiQ9M8D3.
PRIDEiQ9M8D3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G74260.1; AT1G74260.1; AT1G74260.
GeneIDi843766.
GrameneiAT1G74260.1; AT1G74260.1; AT1G74260.
KEGGiath:AT1G74260.

Organism-specific databases

TAIRiAT1G74260.

Phylogenomic databases

eggNOGiENOG410JUJJ. Eukaryota.
KOG1907. Eukaryota.
COG0046. LUCA.
COG0047. LUCA.
HOGENOMiHOG000261358.
InParanoidiQ9M8D3.
KOiK01952.
OMAiLSANWMW.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
BioCyciARA:AT1G74260-MONOMER.
ReactomeiR-ATH-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiQ9M8D3.

Gene expression databases

GenevisibleiQ9M8D3. AT.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "pur4 mutations are lethal to the male, but not the female, gametophyte and affect sporophyte development in Arabidopsis."
    Berthome R., Thomasset M., Maene M., Bourgeois N., Froger N., Budar F.
    Plant Physiol. 147:650-660(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPUR4_ARATH
AccessioniPrimary (citable) accession number: Q9M8D3
Secondary accession number(s): A9Y5J1, F4HTW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 13, 2012
Last modified: February 17, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.