Q9M8D3 (PUR4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase

Gene names

Ordered Locus Names:	At1g74260
ORF Names:	F1O17.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential to the male gametophyte development. Ref.1
Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subcellular location	Plastid › chloroplast. Mitochondrion Ref.1.
Disruption phenotype	Lethal to the male gametophyte. Ref.1
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.
Sequence caution	The sequence AAG52403.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Mitochondrion Plastid
Domain	Glutamine amidotransferase Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW microgametogenesis Inferred from mutant phenotype Ref.1. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR mitochondrion Inferred from direct assay Ref.1. Source: TAIR
Molecular function	ATP binding Inferred from direct assay. Source: TAIR phosphoribosylformylglycinamidine synthase activity Traceable author statement Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 33	33	Chloroplast and mitochondrion Potential
	Chain	34 – 1387	1354	Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial		PRO_0000029880
Regions
	Domain	1121 – 1361	241	Glutamine amidotransferase type-1
	Nucleotide binding	387 – 398	12	ATP Potential
Sites
	Active site	1215	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9M8D3 [UniParc]. Last modified February 8, 2011. Version 2. Checksum: 4BA4E1EB5B922AC1	FASTA	1,387	151,820
	10 20 30 40 50 60 MLLQRSSMSQ LWGSVRMRTS RLSLNRTKAV SLRCSAQPNK PKAAVSTGSF VTADELPSLV 70 80 90 100 110 120 EKPAAEVIHF YRVPLIQESA NAELLKAVQT KISNQIVSLT TEQSFNIGLE SKLKDEKLSV 130 140 150 160 170 180 LKWILQETYE PENLGTDSFL ERKKQEGLHA VIVEVGPRLS FTTAWSTNAV SICRACGLDE 190 200 210 220 230 240 VTRLERSRRY LLFSKEPLLE NQIKEFAAMV HDRMTECVYT QKLVSFETNV VPEEVKYVPV 250 260 270 280 290 300 MEKGRKALEE INQEMGLAFD EQDLQYYTRL FREDIKRDPT NVELFDIAQS NSEHSRHWFF 310 320 330 340 350 360 AGNMVIDGKP MDKSLMQIVK STWEANRNNS VIGFKDNSSA IRGFLVNQLR PLLPGSVCLL 370 380 390 400 410 420 DVSARDLDIL FTAETHNFPC AVAPYPGAET GAGGRIRDTH ATGRGSFVVA STSGYCVGNL 430 440 450 460 470 480 NMEGSYAPWE DSSFQYPSNL ASPLQILIDA SNGASDYGNK FGEPMIQGYT RTFGMRLPSG 490 500 510 520 530 540 DRREWLKPIM FSAGIGQIDH THITKGEPEV GMLVVKIGGP AYRIGMGGGA ASSMVSGQND 550 560 570 580 590 600 AELDFNAVQR GDAEMSQKLY RVVRACIEMG EKNPIISIHD QGAGGNCNVV KEIIYPQGAE 610 620 630 640 650 660 IDIRAVVVGD HTMSVLEIWG AEYQEQDAIL VKAESREILQ SICKRERLSM AVIGTINGGG 670 680 690 700 710 720 RCTLIDSTAA AKCSKEGLPP PPPAVDLELE KVLGDMPKKT FKFNRIAYAR EPLDIAPGIT 730 740 750 760 770 780 LMDALKRVLR LPSVSSKRFL TTKVDRCVTG LVAQQQTVGP LQITLADVAV IAQTFTDLTG 790 800 810 820 830 840 GACAIGEQPI KGLLDPKAMA RLAVGEALTN LVWAKVTALS DVKASGNWMY AAKLEGEGSA 850 860 870 880 890 900 MYDAAIALSE AMIELGIAID GGKDSLSMAA HADGEVVKAP GNLVISAYVT CPDITKTVTP 910 920 930 940 950 960 DLKLGGDDGI LLHVDLAKGK RRLGGSALAQ VFGQIGNDCP DLDDVPYLKN VFDGVQALIA 970 980 990 1000 1010 1020 ENLVSAGHDI SDGGLVVTAL EMAFAGNKGI NLDLASNGIS LFETLFSEEL GLVLEISKTN 1030 1040 1050 1060 1070 1080 LDAVMEKLRA FDVTAEIIGN VTDSPLIEVK VDGITHLSEK TSFLRDMWED TSFQLEKLQR 1090 1100 1110 1120 1130 1140 LASCVEMEKE GLKFRHEPNW KLSFIPSSTN NNYMSQDVKP KVAVIREEGS NGDREMSAAF 1150 1160 1170 1180 1190 1200 YAAGFEPWDV TVSDLLAGDI TLDQFRGIVF VGGFSYADVL DSAKGWAASI RFNEPVLSQF 1210 1220 1230 1240 1250 1260 QEFYKRPDTF SLGICNGCQL MALLGWVPGP QVGGSLDTSQ PRFVHNESGR FECRFTSVTI 1270 1280 1290 1300 1310 1320 KDSPSIMLKG MEGSTLGVWA AHGEGRAYFP DEGVLDHMLH SDLAPLRYCD DDGNVTEAYP 1330 1340 1350 1360 1370 1380 FNLNGSPLGI AAICSPDGRH LAMMPHPERC FLMWQFPWYP TSWDVEKAGP SPWLKMFQNA RDWLESC

References

[1]	"pur4 mutations are lethal to the male, but not the female, gametophyte and affect sporophyte development in Arabidopsis." Berthome R., Thomasset M., Maene M., Bourgeois N., Froger N., Budar F. Plant Physiol. 147:650-660(2008) [PubMed: 18441219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU091297 mRNA. Translation: ABW87767.1. AC020579 Genomic DNA. Translation: AAG52403.1. Sequence problems.
IPI	IPI00884609.
PIR	A96771.
3D structure databases
ProteinModelPortal	Q9M8D3.
SMR	Q9M8D3. Positions 1118-1385.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9M8D3.
Proteomic databases
PRIDE	Q9M8D3.
ProMEX	Q9M8D3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT1G74260 in contig CT485782_GR.
NMPDR	fig|3702.1.peg.6937.
Organism-specific databases
TAIR	At1g74260.
Phylogenomic databases
GeneTree	EPGT00050000011037.
HOGENOM	HBG335309.
InParanoid	Q9M8D3.
PhylomeDB	Q9M8D3.
Gene expression databases
ArrayExpress	Q9M8D3.
Genevestigator	Q9M8D3.
GermOnline	AT1G74260. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_ARATH

Accession

Primary (citable) accession number: Q9M8D3
Secondary accession number(s): A9Y5J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	February 8, 2011
Last modified:	November 16, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program