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Q9M884

- MPI1_ARATH

UniProt

Q9M884 - MPI1_ARATH

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Protein
Mannose-6-phosphate isomerase 1
Gene
PMI1, MEE31, At3g02570, F16B3.20
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. Involved in the ascorbic acid (AsA) biosynthesis. Required during the endosperm development.2 Publications

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Enzyme regulationi

Inhibited by EDTA, Zn2+, Cd2+, Co2+, p-chloromercuribenzoate and L-ascorbic acid (AsA).1 Publication

Kineticsi

  1. KM=41.3 µM for mannose-6-phosphate1 Publication

Vmax=1.89 µmol/min/mg enzyme with mannose-6-phosphate as substrate

pH dependencei

Optimum pH is 7.5.

Temperature dependencei

Optimum temperature is 52 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi124 – 1241Zinc By similarity
Metal bindingi126 – 1261Zinc By similarity
Metal bindingi151 – 1511Zinc By similarity
Metal bindingi288 – 2881Zinc By similarity
Active sitei307 – 3071 By similarity

GO - Molecular functioni

  1. mannose-6-phosphate isomerase activity Source: TAIR
  2. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  2. carbohydrate metabolic process Source: InterPro
  3. embryo development ending in seed dormancy Source: TAIR
  4. response to L-ascorbic acid Source: UniProtKB
  5. response to cadmium ion Source: UniProtKB
  6. response to cobalt ion Source: UniProtKB
  7. response to light stimulus Source: UniProtKB
  8. response to zinc ion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G02570-MONOMER.
MetaCyc:AT3G02570-MONOMER.
ReactomeiREACT_190862. Synthesis of GDP-mannose.
UniPathwayiUPA00126; UER00423.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase 1 (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase 1
Phosphomannose isomerase 1
Short name:
PMI1
Protein MATERNAL EFFECT EMBRYO ARREST 31
Gene namesi
Name:PMI1
Synonyms:MEE31
Ordered Locus Names:At3g02570
ORF Names:F16B3.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G02570.

Pathology & Biotechi

Disruption phenotypei

Endosperm development arrested.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Mannose-6-phosphate isomerase 1
PRO_0000420339Add
BLAST

Proteomic databases

PaxDbiQ9M884.
PRIDEiQ9M884.

Expressioni

Tissue specificityi

Constitutively expressed in both vegetative and reproductive organs under normal growth conditions (at protein level).1 Publication

Inductioni

By light (at the protein level). Down-regulated by dark (at the protein level). Down-regulated by DCMU, an exogenous photosynthesis inhibitor.1 Publication

Gene expression databases

GenevestigatoriQ9M884.

Interactioni

Protein-protein interaction databases

IntActiQ9M884. 1 interaction.
STRINGi3702.AT3G02570.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9M884.
SMRiQ9M884. Positions 19-426.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1482.
HOGENOMiHOG000241277.
InParanoidiQ9M884.
KOiK01809.
OMAiWITENPK.
PhylomeDBiQ9M884.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR10309. PTHR10309. 1 hit.
PfamiPF01238. PMI_typeI. 1 hit.
[Graphical view]
PIRSFiPIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSiPR00714. MAN6PISMRASE.
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR00218. manA. 1 hit.
PROSITEiPS00965. PMI_I_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M884-1 [UniParc]FASTAAdd to Basket

« Hide

MEIATVVKAN GGCEADRRRL RRLRCSVKDY DWGKIGSDSL VYRVYAANSD    50
YEIDPTRPYA ELWMGTHESG PSYLEDADGS NGVTLRSWIT ENPKSLGNRV 100
LEKWGCDLPF LFKVLSVARP LSIQAHPDKK LAKKMHKAHP NLYKDDNHKP 150
EMALAYTQFE ALCGFIPLQE LKSVIRAIPE IEELVGSEEA NQVFCITEHD 200
EEKVKSVVRT IFTLLMSADA DTTKKIVSKL KRRLHMESQE RQLTDKERLV 250
LKLEKQYPND IGVISAFFFN YVKLNPGEAL YLGANEPHAY LFGECLEVMA 300
TSDNVVRAGL TSKPLDIQTL CSMLSYKLGY PEILKGTRIR PYITRYLPPF 350
EEFEVDLCDL PSGASTVFPS VPGPSLLLVL QGEGRMSTEA SADGISMGDV 400
LFVPADTEIH LRSSSDLKLY RAGINSRFLF PL 432
Length:432
Mass (Da):48,566
Last modified:October 1, 2000 - v1
Checksum:i9BF364B9BCF8FC9B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC021640 Genomic DNA. Translation: AAF32464.1.
CP002686 Genomic DNA. Translation: AEE73831.1.
AY070447 mRNA. Translation: AAL49850.1.
AY096564 mRNA. Translation: AAM20214.1.
RefSeqiNP_186906.1. NM_111125.3.
UniGeneiAt.18813.
At.24147.

Genome annotation databases

EnsemblPlantsiAT3G02570.1; AT3G02570.1; AT3G02570.
GeneIDi820656.
KEGGiath:AT3G02570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC021640 Genomic DNA. Translation: AAF32464.1 .
CP002686 Genomic DNA. Translation: AEE73831.1 .
AY070447 mRNA. Translation: AAL49850.1 .
AY096564 mRNA. Translation: AAM20214.1 .
RefSeqi NP_186906.1. NM_111125.3.
UniGenei At.18813.
At.24147.

3D structure databases

ProteinModelPortali Q9M884.
SMRi Q9M884. Positions 19-426.
ModBasei Search...

Protein-protein interaction databases

IntActi Q9M884. 1 interaction.
STRINGi 3702.AT3G02570.1-P.

Proteomic databases

PaxDbi Q9M884.
PRIDEi Q9M884.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G02570.1 ; AT3G02570.1 ; AT3G02570 .
GeneIDi 820656.
KEGGi ath:AT3G02570.

Organism-specific databases

TAIRi AT3G02570.

Phylogenomic databases

eggNOGi COG1482.
HOGENOMi HOG000241277.
InParanoidi Q9M884.
KOi K01809.
OMAi WITENPK.
PhylomeDBi Q9M884.

Enzyme and pathway databases

UniPathwayi UPA00126 ; UER00423 .
BioCyci ARA:AT3G02570-MONOMER.
MetaCyc:AT3G02570-MONOMER.
Reactomei REACT_190862. Synthesis of GDP-mannose.

Miscellaneous databases

PROi Q9M884.

Gene expression databases

Genevestigatori Q9M884.

Family and domain databases

Gene3Di 2.60.120.10. 3 hits.
InterProi IPR001250. Man6P_Isoase-1.
IPR016305. Mannose-6-P_Isomerase.
IPR018050. Pmannose_isomerase-type1_CS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR10309. PTHR10309. 1 hit.
Pfami PF01238. PMI_typeI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001480. Mannose-6-phosphate_isomerase. 1 hit.
PRINTSi PR00714. MAN6PISMRASE.
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR00218. manA. 1 hit.
PROSITEi PS00965. PMI_I_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis."
    Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S., Capron A., Xie L.-F., Ye D., Sundaresan V.
    Development 132:603-614(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Arabidopsis phosphomannose isomerase 1, but not phosphomannose isomerase 2, is essential for ascorbic acid biosynthesis."
    Maruta T., Yonemitsu M., Yabuta Y., Tamoi M., Ishikawa T., Shigeoka S.
    J. Biol. Chem. 283:28842-28851(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY, INDUCTION.
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMPI1_ARATH
AccessioniPrimary (citable) accession number: Q9M884
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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