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Protein

Mannose-6-phosphate isomerase 1

Gene

PMI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. Involved in the ascorbic acid (AsA) biosynthesis. Required during the endosperm development.2 Publications

Catalytic activityi

D-mannose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Inhibited by EDTA, Zn2+, Cd2+, Co2+, p-chloromercuribenzoate and L-ascorbic acid (AsA).1 Publication

Kineticsi

  1. KM=41.3 µM for mannose-6-phosphate1 Publication
  1. Vmax=1.89 µmol/min/mg enzyme with mannose-6-phosphate as substrate1 Publication

pH dependencei

Optimum pH is 7.5.1 Publication

Temperature dependencei

Optimum temperature is 52 degrees Celsius.1 Publication

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Mannose-6-phosphate isomerase 1 (PMI1), Mannose-6-phosphate isomerase 2 (PMI2)
  2. Phosphomannomutase (PMM), Phosphomannomutase (AXX17_At2g43390)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-mannose 1-phosphate from D-fructose 6-phosphate, the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi124ZincBy similarity1
Metal bindingi126ZincBy similarity1
Metal bindingi151ZincBy similarity1
Metal bindingi288ZincBy similarity1
Active sitei307By similarity1

GO - Molecular functioni

  • mannose-6-phosphate isomerase activity Source: TAIR
  • zinc ion binding Source: InterPro

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cell wall mannoprotein biosynthetic process Source: GO_Central
  • embryo development ending in seed dormancy Source: TAIR
  • GDP-mannose biosynthetic process Source: GO_Central
  • protein glycosylation Source: GO_Central
  • response to cadmium ion Source: UniProtKB
  • response to cobalt ion Source: UniProtKB
  • response to L-ascorbic acid Source: UniProtKB
  • response to light stimulus Source: UniProtKB
  • response to zinc ion Source: UniProtKB

Keywordsi

Molecular functionIsomerase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT3G02570-MONOMER
MetaCyc:AT3G02570-MONOMER
BRENDAi5.3.1.8 399
ReactomeiR-ATH-446205 Synthesis of GDP-mannose
SABIO-RKiQ9M884
UniPathwayiUPA00126; UER00423

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-6-phosphate isomerase 1 (EC:5.3.1.8)
Alternative name(s):
Phosphohexomutase 1
Phosphomannose isomerase 1
Short name:
PMI1
Protein MATERNAL EFFECT EMBRYO ARREST 31
Gene namesi
Name:PMI1
Synonyms:MEE31
Ordered Locus Names:At3g02570
ORF Names:F16B3.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT3G02570
TAIRilocus:2076864 AT3G02570

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Endosperm development arrested.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004203391 – 432Mannose-6-phosphate isomerase 1Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9M884
PRIDEiQ9M884

PTM databases

iPTMnetiQ9M884

Expressioni

Tissue specificityi

Constitutively expressed in both vegetative and reproductive organs under normal growth conditions (at protein level).1 Publication

Inductioni

By light (at the protein level). Down-regulated by dark (at the protein level). Down-regulated by DCMU, an exogenous photosynthesis inhibitor.1 Publication

Gene expression databases

ExpressionAtlasiQ9M884 baseline and differential
GenevisibleiQ9M884 AT

Interactioni

Protein-protein interaction databases

BioGridi5990, 1 interactor
IntActiQ9M884, 1 interactor
STRINGi3702.AT3G02570.1

Structurei

3D structure databases

ProteinModelPortaliQ9M884
SMRiQ9M884
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2757 Eukaryota
COG1482 LUCA
HOGENOMiHOG000241277
InParanoidiQ9M884
KOiK01809
OMAiMGTHPSN
OrthoDBiEOG09360AGB
PhylomeDBiQ9M884

Family and domain databases

Gene3Di2.60.120.10, 4 hits
InterProiView protein in InterPro
IPR001250 Man6P_Isoase-1
IPR016305 Mannose-6-P_Isomerase
IPR018050 Pmannose_isomerase-type1_CS
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PANTHERiPTHR10309 PTHR10309, 1 hit
PfamiView protein in Pfam
PF01238 PMI_typeI, 1 hit
PIRSFiPIRSF001480 Mannose-6-phosphate_isomerase, 1 hit
PRINTSiPR00714 MAN6PISMRASE
SUPFAMiSSF51182 SSF51182, 1 hit
TIGRFAMsiTIGR00218 manA, 1 hit
PROSITEiView protein in PROSITE
PS00965 PMI_I_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q9M884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIATVVKAN GGCEADRRRL RRLRCSVKDY DWGKIGSDSL VYRVYAANSD
60 70 80 90 100
YEIDPTRPYA ELWMGTHESG PSYLEDADGS NGVTLRSWIT ENPKSLGNRV
110 120 130 140 150
LEKWGCDLPF LFKVLSVARP LSIQAHPDKK LAKKMHKAHP NLYKDDNHKP
160 170 180 190 200
EMALAYTQFE ALCGFIPLQE LKSVIRAIPE IEELVGSEEA NQVFCITEHD
210 220 230 240 250
EEKVKSVVRT IFTLLMSADA DTTKKIVSKL KRRLHMESQE RQLTDKERLV
260 270 280 290 300
LKLEKQYPND IGVISAFFFN YVKLNPGEAL YLGANEPHAY LFGECLEVMA
310 320 330 340 350
TSDNVVRAGL TSKPLDIQTL CSMLSYKLGY PEILKGTRIR PYITRYLPPF
360 370 380 390 400
EEFEVDLCDL PSGASTVFPS VPGPSLLLVL QGEGRMSTEA SADGISMGDV
410 420 430
LFVPADTEIH LRSSSDLKLY RAGINSRFLF PL
Length:432
Mass (Da):48,566
Last modified:October 1, 2000 - v1
Checksum:i9BF364B9BCF8FC9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC021640 Genomic DNA Translation: AAF32464.1
CP002686 Genomic DNA Translation: AEE73831.1
AY070447 mRNA Translation: AAL49850.1
AY096564 mRNA Translation: AAM20214.1
RefSeqiNP_186906.1, NM_111125.4
UniGeneiAt.18813
At.24147

Genome annotation databases

EnsemblPlantsiAT3G02570.1; AT3G02570.1; AT3G02570
GeneIDi820656
GrameneiAT3G02570.1; AT3G02570.1; AT3G02570
KEGGiath:AT3G02570

Entry informationi

Entry nameiMPI1_ARATH
AccessioniPrimary (citable) accession number: Q9M884
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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