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Protein

Tyrosine--tRNA ligase, chloroplastic/mitochondrial

Gene

EMB2768

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).By similarity

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118TyrosineBy similarity1
Binding sitei122ATPBy similarity1
Binding sitei162TyrosineBy similarity1
Binding sitei256TyrosineBy similarity1
Binding sitei260TyrosineBy similarity1
Binding sitei263TyrosineBy similarity1
Binding sitei282TyrosineBy similarity1
Binding sitei321ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • tRNA aminoacylation Source: GO_Central
  • tyrosyl-tRNA aminoacylation Source: InterPro

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.1By similarity)
Alternative name(s):
Protein EMBRYO DEFECTIVE 27681 Publication
Protein EMBRYONIC FACTOR 311 Publication
Tyrosyl-tRNA synthetaseCurated
Short name:
TyrRSCurated
Gene namesi
Name:EMB27681 Publication
Synonyms:FAC311 Publication
Ordered Locus Names:At3g02660Imported
ORF Names:F16B3.29Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G02660
TAIRilocus:2076879 AT3G02660

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo defective. Developmental arrest of the embryo at the preglobular stage.1 Publication1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004335551 – 511Tyrosine--tRNA ligase, chloroplastic/mitochondrialCuratedAdd BLAST511
Transit peptidei1 – ?Chloroplast and mitochondrionCurated

Proteomic databases

PaxDbiQ9M876
PRIDEiQ9M876

Expressioni

Gene expression databases

ExpressionAtlasiQ9M876 baseline and differential
GenevisibleiQ9M876 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G02660.1

Structurei

3D structure databases

ProteinModelPortaliQ9M876
SMRiQ9M876
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini444 – 510S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi123 – 132"HIGH" regionCurated10
Motifi318 – 322"KMSKS" regionCurated5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2623 Eukaryota
COG0162 LUCA
HOGENOMiHOG000242790
InParanoidiQ9M876
KOiK01866
OMAiVNYMMAK
OrthoDBiEOG093607YA
PhylomeDBiQ9M876

Family and domain databases

CDDicd00165 S4, 1 hit
cd00805 TyrRS_core, 1 hit
Gene3Di3.10.290.10, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_02006 Tyr_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002942 S4_RNA-bd
IPR036986 S4_RNA-bd_sf
IPR002307 Tyr-tRNA-ligase
IPR024088 Tyr-tRNA-ligase_bac-type
IPR024107 Tyr-tRNA-ligase_bac_1
PANTHERiPTHR11766 PTHR11766, 1 hit
PfamiView protein in Pfam
PF01479 S4, 1 hit
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01040 TRNASYNTHTYR
SMARTiView protein in SMART
SM00363 S4, 1 hit
TIGRFAMsiTIGR00234 tyrS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYATGITFA SRSILPICSR TFLSPLRVAS LLVFPEKSSA TFFRRVQVPH
60 70 80 90 100
LFSTSTTTLF SSVKCSIHST SSPETENQAV FRPNVVDILE ERGLLESITS
110 120 130 140 150
ENLRSACSDP KVAPLRVYCG FDPTAESLHL GNLLGIIVLS WFQRCGHQAV
160 170 180 190 200
GLIGGATGRV GDPSGKSLER PELDADTLEK NIAGITKIII KILGSNPSPG
210 220 230 240 250
GSYVIFNNYD WWKDMTMLDF LNKVGRFARV GTMMAKESVK KRLESEQGMS
260 270 280 290 300
YTEFTYQLLQ AYDFLHLFKN EGINVQIGGS DQWGNITAGT DLIRKILQAE
310 320 330 340 350
EAAYGLTFPL LLKNDGTKFG KSEDGAIWLS PSMLSPYKFY QYFFSVPDVD
360 370 380 390 400
VIRFLKTLTF LSLDEIKILE DQMSKPGYVP NTAQIKLAEE VTRFVHGEEG
410 420 430 440 450
LKEAIKATEA LRPGAETKLD WNLIERIAED IPSCSLPIDR VSGLSIVDLS
460 470 480 490 500
VSAGLFESKS AARRMLKQGG FYMNNERVDD ENKRVDEEDI VEGRGLVLSA
510
GKKNKVVVRI S
Length:511
Mass (Da):56,619
Last modified:October 1, 2000 - v1
Checksum:i43DECC2C66FF95DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC021640 Genomic DNA Translation: AAF32473.1
CP002686 Genomic DNA Translation: AEE73845.1
CP002686 Genomic DNA Translation: ANM63412.1
AK221609 mRNA Translation: BAD95182.1
BT029296 mRNA Translation: ABK32110.1
RefSeqiNP_001325501.1, NM_001337429.1
NP_186915.1, NM_111134.5
UniGeneiAt.41099

Genome annotation databases

EnsemblPlantsiAT3G02660.1; AT3G02660.1; AT3G02660
AT3G02660.2; AT3G02660.2; AT3G02660
GeneIDi821282
GrameneiAT3G02660.1; AT3G02660.1; AT3G02660
AT3G02660.2; AT3G02660.2; AT3G02660
KEGGiath:AT3G02660

Similar proteinsi

Entry informationi

Entry nameiSYYM_ARATH
AccessioniPrimary (citable) accession number: Q9M876
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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