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Protein

Tyrosine--tRNA ligase, chloroplastic/mitochondrial

Gene

EMB2768

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).By similarity

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118TyrosineBy similarity1
Binding sitei256TyrosineBy similarity1
Binding sitei260TyrosineBy similarity1
Binding sitei263TyrosineBy similarity1
Binding sitei282TyrosineBy similarity1
Binding sitei321ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • tRNA aminoacylation Source: GO_Central
  • tyrosyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciARA:AT3G02660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.1Curated)
Alternative name(s):
Protein EMBRYO DEFECTIVE 27681 Publication
Protein EMBRYONIC FACTOR 311 Publication
Tyrosyl-tRNA synthetaseCurated
Short name:
TyrRSCurated
Gene namesi
Name:EMB27681 Publication
Synonyms:FAC311 Publication
Ordered Locus Names:At3g02660Imported
ORF Names:F16B3.29Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G02660.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: GO_Central
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo defective. Developmental arrest of the embryo at the preglobular stage.1 Publication1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004335551 – 511Tyrosine--tRNA ligase, chloroplastic/mitochondrialCuratedAdd BLAST511
Transit peptidei1 – ?Chloroplast and mitochondrionCurated

Proteomic databases

PaxDbiQ9M876.
PRIDEiQ9M876.

Expressioni

Gene expression databases

GenevisibleiQ9M876. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G02660.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M876.
SMRiQ9M876.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini444 – 510S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi123 – 132"HIGH" regionCurated10
Motifi318 – 322"KMSKS" regionCurated5

Sequence similaritiesi

Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2623. Eukaryota.
COG0162. LUCA.
HOGENOMiHOG000242790.
InParanoidiQ9M876.
KOiK01866.
OMAiGKHFPVN.
OrthoDBiEOG093607YA.
PhylomeDBiQ9M876.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M876-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYATGITFA SRSILPICSR TFLSPLRVAS LLVFPEKSSA TFFRRVQVPH
60 70 80 90 100
LFSTSTTTLF SSVKCSIHST SSPETENQAV FRPNVVDILE ERGLLESITS
110 120 130 140 150
ENLRSACSDP KVAPLRVYCG FDPTAESLHL GNLLGIIVLS WFQRCGHQAV
160 170 180 190 200
GLIGGATGRV GDPSGKSLER PELDADTLEK NIAGITKIII KILGSNPSPG
210 220 230 240 250
GSYVIFNNYD WWKDMTMLDF LNKVGRFARV GTMMAKESVK KRLESEQGMS
260 270 280 290 300
YTEFTYQLLQ AYDFLHLFKN EGINVQIGGS DQWGNITAGT DLIRKILQAE
310 320 330 340 350
EAAYGLTFPL LLKNDGTKFG KSEDGAIWLS PSMLSPYKFY QYFFSVPDVD
360 370 380 390 400
VIRFLKTLTF LSLDEIKILE DQMSKPGYVP NTAQIKLAEE VTRFVHGEEG
410 420 430 440 450
LKEAIKATEA LRPGAETKLD WNLIERIAED IPSCSLPIDR VSGLSIVDLS
460 470 480 490 500
VSAGLFESKS AARRMLKQGG FYMNNERVDD ENKRVDEEDI VEGRGLVLSA
510
GKKNKVVVRI S
Length:511
Mass (Da):56,619
Last modified:October 1, 2000 - v1
Checksum:i43DECC2C66FF95DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC021640 Genomic DNA. Translation: AAF32473.1.
CP002686 Genomic DNA. Translation: AEE73845.1.
AK221609 mRNA. Translation: BAD95182.1.
BT029296 mRNA. Translation: ABK32110.1.
RefSeqiNP_186915.1. NM_111134.5.
UniGeneiAt.41099.

Genome annotation databases

EnsemblPlantsiAT3G02660.1; AT3G02660.1; AT3G02660.
GeneIDi821282.
GrameneiAT3G02660.1; AT3G02660.1; AT3G02660.
KEGGiath:AT3G02660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC021640 Genomic DNA. Translation: AAF32473.1.
CP002686 Genomic DNA. Translation: AEE73845.1.
AK221609 mRNA. Translation: BAD95182.1.
BT029296 mRNA. Translation: ABK32110.1.
RefSeqiNP_186915.1. NM_111134.5.
UniGeneiAt.41099.

3D structure databases

ProteinModelPortaliQ9M876.
SMRiQ9M876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G02660.1.

Proteomic databases

PaxDbiQ9M876.
PRIDEiQ9M876.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G02660.1; AT3G02660.1; AT3G02660.
GeneIDi821282.
GrameneiAT3G02660.1; AT3G02660.1; AT3G02660.
KEGGiath:AT3G02660.

Organism-specific databases

TAIRiAT3G02660.

Phylogenomic databases

eggNOGiKOG2623. Eukaryota.
COG0162. LUCA.
HOGENOMiHOG000242790.
InParanoidiQ9M876.
KOiK01866.
OMAiGKHFPVN.
OrthoDBiEOG093607YA.
PhylomeDBiQ9M876.

Enzyme and pathway databases

BioCyciARA:AT3G02660-MONOMER.

Miscellaneous databases

PROiQ9M876.

Gene expression databases

GenevisibleiQ9M876. AT.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYYM_ARATH
AccessioniPrimary (citable) accession number: Q9M876
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.