Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxisome biogenesis protein 12

Gene

PEX12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for peroxisome biogenesis and for PTS1- and PTS2-dependent protein import into peroxisomes. Essential for the peroxisomal targeting of PEX7. Required for the export/release of receptors on the peroxisome membrane. Acts as an E3 ubiquitin-protein ligase involved in monoubiquitination of PEX5.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri339 – 378RING-type; degenerateAdd BLAST40

GO - Molecular functioni

GO - Biological processi

  • embryonic morphogenesis Source: TAIR
  • fatty acid beta-oxidation Source: TAIR
  • peroxisome organization Source: TAIR
  • protein import into peroxisome matrix Source: TAIR
  • protein monoubiquitination Source: TAIR

Keywordsi

Biological processPeroxisome biogenesis, Protein transport, Transport, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-8866654. E3 ubiquitin ligases ubiquitinate target proteins.

Protein family/group databases

TCDBi3.A.20.1.2. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisome biogenesis protein 12
Alternative name(s):
Peroxin-12
Short name:
AtPEX12
Pex12p
Protein ABERRANT PEROXISOME MORPHOLOGY 4
Gene namesi
Name:PEX12
Synonyms:APM4
Ordered Locus Names:At3g04460
ORF Names:T27C4.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G04460.
TAIRilocus:2100890. AT3G04460.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei212 – 231HelicalSequence analysisAdd BLAST20
Transmembranei270 – 294HelicalSequence analysisAdd BLAST25

Keywords - Cellular componenti

Membrane, Peroxisome

Pathology & Biotechi

Disruption phenotypei

Embryo lethality at the heart stage.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170R → K in apm4; reduced protein transport to peroxisome and repressed plant growth. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186151 – 393Peroxisome biogenesis protein 12Add BLAST393

Proteomic databases

PaxDbiQ9M841.

Expressioni

Tissue specificityi

Expressed in young seedlings, roots, leaves, seeds and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ9M841. baseline and differential.
GenevisibleiQ9M841. AT.

Interactioni

Subunit structurei

Interacts (via C-terminus) with PEX7. Interacts with DSK2a and DSK2b.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4937. 2 interactors.
IntActiQ9M841. 7 interactors.
STRINGi3702.AT3G04460.1.

Structurei

3D structure databases

ProteinModelPortaliQ9M841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi311 – 334Pro-richAdd BLAST24

Sequence similaritiesi

Belongs to the pex2/pex10/pex12 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri339 – 378RING-type; degenerateAdd BLAST40

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG0826. Eukaryota.
ENOG410Y4Q2. LUCA.
HOGENOMiHOG000038427.
InParanoidiQ9M841.
KOiK13345.
OMAiWWYSSEN.
OrthoDBiEOG09360CCQ.
PhylomeDBiQ9M841.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR017375. PEX12.
IPR006845. Pex_N.
IPR013083. Znf_RING/FYVE/PHD.
PANTHERiPTHR12888. PTHR12888. 1 hit.
PfamiView protein in Pfam
PF04757. Pex2_Pex12. 1 hit.
PIRSFiPIRSF038074. Peroxisome_assembly_p12. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9M841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFQVGGEGT RPTFFEMAAA QQLPASLRAA LTYSLGVFAL RRSFLHKILD
60 70 80 90 100
YEDEFFAALM LILEGHSLRT TDGSFAESLY GLRRKSARLR LRKDSARKDS
110 120 130 140 150
SEEVQHSGLE KRQRILSVVF LVVLPYFKSK LHAIYNKERE ARLRESLWGA
160 170 180 190 200
EDQGFDEADF FTGDDSIVSR EPSGNEELSV RVQLATKIKK FIAVCYPWIH
210 220 230 240 250
ASSEGLSFTY QLLYLLDATG FYSLGLQALG IQVCRATGQE LMDTSSRISK
260 270 280 290 300
IRNHERERLR GPPWLKTVQG ALLSCSYAVL DYAQTGLIAA VFIFKMMEWW
310 320 330 340 350
YQSAEERLSA PTVYPPPPPP PAPKMAKEGI PLPPDRSLCA LCLQKRANPS
360 370 380 390
VVTVSGFVFC YSCVFKYVSK YKRCPVTLIP ASVDQIRRLF QDT
Length:393
Mass (Da):44,400
Last modified:October 14, 2008 - v2
Checksum:iBEF9C580A93F52A7
GO

Sequence cautioni

Q9M841: The sequence AAF63777 differs from that shown. Reason: Erroneous gene model prediction.Curated
Q9M841: The sequence BAC42305 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
Q9M841: The sequence BX824646 differs from that shown. Reason: Frameshift at position 250.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96A → S in BX824646 (PubMed:14993207).Curated1
Sequence conflicti392D → E in BX824646 (PubMed:14993207).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC022287 Genomic DNA. Translation: AAF63777.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE74085.1.
BX824646 mRNA. No translation available.
AK117651 mRNA. Translation: BAC42305.1. Different initiation.
RefSeqiNP_187096.2. NM_111317.4.
UniGeneiAt.18851.

Genome annotation databases

EnsemblPlantsiAT3G04460.1; AT3G04460.1; AT3G04460.
GeneIDi819602.
GrameneiAT3G04460.1; AT3G04460.1; AT3G04460.
KEGGiath:AT3G04460.

Similar proteinsi

Entry informationi

Entry nameiPEX12_ARATH
AccessioniPrimary (citable) accession number: Q9M841
Secondary accession number(s): Q8GYG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 14, 2008
Last modified: September 27, 2017
This is version 115 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families