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Protein

Protein NRT1/ PTR FAMILY 1.2

Gene

NPF1.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Low-affinity nitrate transporter involved in xylem-to-phloem transfer for redistributing nitrate into developing leaves. Not involved in dipeptides transport.1 Publication

Kineticsi

  1. KM=7.2 mM for nitrate1 Publication

    GO - Molecular functioni

    • low-affinity nitrate transmembrane transporter activity Source: TAIR

    GO - Biological processi

    • nitrate transport Source: TAIR

    Enzyme and pathway databases

    ReactomeiR-ATH-427975 Proton/oligopeptide cotransporters
    R-ATH-6798695 Neutrophil degranulation

    Protein family/group databases

    TCDBi2.A.17.3.13 the proton-dependent oligopeptide transporter (pot/ptr) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 1.2
    Short name:
    AtNPF1.2
    Alternative name(s):
    Nitrate transporter 1.11
    Gene namesi
    Name:NPF1.2
    Synonyms:NRT1.11
    Ordered Locus Names:At1g52190
    ORF Names:F9I5.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G52190
    TAIRilocus:2037452 AT1G52190

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
    Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
    Transmembranei138 – 158HelicalSequence analysisAdd BLAST21
    Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
    Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
    Transmembranei374 – 394HelicalSequence analysisAdd BLAST21
    Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
    Transmembranei460 – 480HelicalSequence analysisAdd BLAST21
    Transmembranei496 – 516HelicalSequence analysisAdd BLAST21
    Transmembranei544 – 564HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Nrt1.11 and nrt1.12 double mutant is defective in high-nitrate-enhanced growth.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003241231 – 607Protein NRT1/ PTR FAMILY 1.2Add BLAST607

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei601PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9M817
    PRIDEiQ9M817

    PTM databases

    iPTMnetiQ9M817

    Expressioni

    Tissue specificityi

    Expressed in shoots, stems, leaves, flowers and siliques. Mainly detected in larger expanded leaves, in the companion cells of major veins.2 Publications

    Gene expression databases

    ExpressionAtlasiQ9M817 baseline and differential
    GenevisibleiQ9M817 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi26874, 4 interactors
    STRINGi3702.AT1G52190.1

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M817
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi583 – 586Poly-Glu4

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237 Eukaryota
    COG3104 LUCA
    HOGENOMiHOG000237399
    InParanoidiQ9M817
    OMAiIMMSINT
    OrthoDBiEOG093605FJ
    PhylomeDBiQ9M817

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    IPR000109 POT_fam
    PANTHERiPTHR11654 PTHR11654, 1 hit
    PfamiView protein in Pfam
    PF00854 PTR2, 1 hit
    SUPFAMiSSF103473 SSF103473, 2 hits

    Sequencei

    Sequence statusi: Complete.

    Q9M817-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MENPPNETEA KQIQTNEGKK TKGGIITMPF IIANEAFEKV ASYGLLPNMI
    60 70 80 90 100
    MYLIRDYRFG VAKGTNVLFM WSAASNFTPL LGAFLSDSYL GRFLTISIAS
    110 120 130 140 150
    LSSFLGMVLL WLTAMLPQVK PSPCDPTAAG SHCGSSTASQ LALLYSAFAL
    160 170 180 190 200
    ISIGSGGIRP CSLAFGADQL DNKENPKNER VLESFFGWYY ASSAVAVLIA
    210 220 230 240 250
    FTGIVYIQEH LGWKIGFGVP AVLMLIAALL FILASPLYVT RGVTKSLFTG
    260 270 280 290 300
    LAQAIVAAYK KRKLSLPDHH DSFDCYYHMK DSEIKAPSQK LRFLNKACLI
    310 320 330 340 350
    SNREEEIGSD GFALNPWRLC TTDKVEELKA LIKVIPIWST GIMMSINTSQ
    360 370 380 390 400
    SSFQLLQATS MDRRLSRHGS SFQVPAGSFG MFTIIALALW VILYDRAVIP
    410 420 430 440 450
    LASKIRGRPF RLSVKLRMGL GLFMSFLAMA ISAMVESFRR KKAISQGYAN
    460 470 480 490 500
    NSNAVVDISA MWLVPQYVLH GLAEALTAIG QTEFFYTEFP KSMSSIAASL
    510 520 530 540 550
    FGLGMAVASL LASVVLNAVN ELTSRNGKES WVSDNINKGH YNYYYWVLAI
    560 570 580 590 600
    MSFINVIYYV ICSWSYGPLV DQVRNGRVNG VREEEELIDI VGKGFEKEDL

    SPVVKTN
    Length:607
    Mass (Da):66,904
    Last modified:October 1, 2000 - v1
    Checksum:iB8355650ADF0FBE4
    GO

    Sequence cautioni

    The sequence AAL25616 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti22K → M in AAM61194 (Ref. 3) Curated1
    Sequence conflicti244T → S in AAM61194 (Ref. 3) Curated1
    Sequence conflicti273F → I in AAM61194 (Ref. 3) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC022354 Genomic DNA Translation: AAF29404.1
    CP002684 Genomic DNA Translation: AEE32766.1
    AY084631 mRNA Translation: AAM61194.1
    AY058204 mRNA Translation: AAL25616.1 Different initiation.
    AY098981 mRNA Translation: AAM19991.1
    PIRiH96561
    RefSeqiNP_175630.1, NM_104099.4
    UniGeneiAt.25542

    Genome annotation databases

    EnsemblPlantsiAT1G52190.1; AT1G52190.1; AT1G52190
    GeneIDi841649
    GrameneiAT1G52190.1; AT1G52190.1; AT1G52190
    KEGGiath:AT1G52190

    Similar proteinsi

    Entry informationi

    Entry nameiPTR6_ARATH
    AccessioniPrimary (citable) accession number: Q9M817
    Secondary accession number(s): Q8LFV0, Q93Z23
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: October 1, 2000
    Last modified: April 25, 2018
    This is version 97 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families
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