Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein NRT1/ PTR FAMILY 1.2

Gene

NPF1.2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity nitrate transporter involved in xylem-to-phloem transfer for redistributing nitrate into developing leaves. Not involved in dipeptides transport.1 Publication

Kineticsi

  1. KM=7.2 mM for nitrate1 Publication

    GO - Molecular functioni

    • low-affinity nitrate transmembrane transporter activity Source: TAIR

    GO - Biological processi

    • low-affinity nitrate transport Source: TAIR
    Complete GO annotation...

    Enzyme and pathway databases

    ReactomeiR-ATH-427975. Proton/oligopeptide cotransporters.

    Protein family/group databases

    TCDBi2.A.17.3.13. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 1.2
    Short name:
    AtNPF1.2
    Alternative name(s):
    Nitrate transporter 1.11
    Gene namesi
    Name:NPF1.2
    Synonyms:NRT1.11
    Ordered Locus Names:At1g52190
    ORF Names:F9I5.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G52190.

    Subcellular locationi

    • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei65 – 8521HelicalSequence analysisAdd
    BLAST
    Transmembranei96 – 11621HelicalSequence analysisAdd
    BLAST
    Transmembranei138 – 15821HelicalSequence analysisAdd
    BLAST
    Transmembranei185 – 20521HelicalSequence analysisAdd
    BLAST
    Transmembranei215 – 23521HelicalSequence analysisAdd
    BLAST
    Transmembranei374 – 39421HelicalSequence analysisAdd
    BLAST
    Transmembranei418 – 43821HelicalSequence analysisAdd
    BLAST
    Transmembranei460 – 48021HelicalSequence analysisAdd
    BLAST
    Transmembranei496 – 51621HelicalSequence analysisAdd
    BLAST
    Transmembranei544 – 56421HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Nrt1.11 and nrt1.12 double mutant is defective in high-nitrate-enhanced growth.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 607607Protein NRT1/ PTR FAMILY 1.2PRO_0000324123Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei601 – 6011PhosphoserineCombined sources

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9M817.
    PRIDEiQ9M817.

    PTM databases

    iPTMnetiQ9M817.

    Expressioni

    Tissue specificityi

    Expressed in shoots, stems, leaves, flowers and siliques. Mainly detected in larger expanded leaves, in the companion cells of major veins.2 Publications

    Gene expression databases

    GenevisibleiQ9M817. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi26874. 4 interactions.
    STRINGi3702.AT1G52190.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9M817.
    SMRiQ9M817. Positions 19-564.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi583 – 5864Poly-Glu

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237399.
    InParanoidiQ9M817.
    OMAiGGKESWV.
    OrthoDBiEOG093605FJ.
    PhylomeDBiQ9M817.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9M817-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MENPPNETEA KQIQTNEGKK TKGGIITMPF IIANEAFEKV ASYGLLPNMI
    60 70 80 90 100
    MYLIRDYRFG VAKGTNVLFM WSAASNFTPL LGAFLSDSYL GRFLTISIAS
    110 120 130 140 150
    LSSFLGMVLL WLTAMLPQVK PSPCDPTAAG SHCGSSTASQ LALLYSAFAL
    160 170 180 190 200
    ISIGSGGIRP CSLAFGADQL DNKENPKNER VLESFFGWYY ASSAVAVLIA
    210 220 230 240 250
    FTGIVYIQEH LGWKIGFGVP AVLMLIAALL FILASPLYVT RGVTKSLFTG
    260 270 280 290 300
    LAQAIVAAYK KRKLSLPDHH DSFDCYYHMK DSEIKAPSQK LRFLNKACLI
    310 320 330 340 350
    SNREEEIGSD GFALNPWRLC TTDKVEELKA LIKVIPIWST GIMMSINTSQ
    360 370 380 390 400
    SSFQLLQATS MDRRLSRHGS SFQVPAGSFG MFTIIALALW VILYDRAVIP
    410 420 430 440 450
    LASKIRGRPF RLSVKLRMGL GLFMSFLAMA ISAMVESFRR KKAISQGYAN
    460 470 480 490 500
    NSNAVVDISA MWLVPQYVLH GLAEALTAIG QTEFFYTEFP KSMSSIAASL
    510 520 530 540 550
    FGLGMAVASL LASVVLNAVN ELTSRNGKES WVSDNINKGH YNYYYWVLAI
    560 570 580 590 600
    MSFINVIYYV ICSWSYGPLV DQVRNGRVNG VREEEELIDI VGKGFEKEDL

    SPVVKTN
    Length:607
    Mass (Da):66,904
    Last modified:October 1, 2000 - v1
    Checksum:iB8355650ADF0FBE4
    GO

    Sequence cautioni

    The sequence AAL25616 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221K → M in AAM61194 (Ref. 3) Curated
    Sequence conflicti244 – 2441T → S in AAM61194 (Ref. 3) Curated
    Sequence conflicti273 – 2731F → I in AAM61194 (Ref. 3) Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC022354 Genomic DNA. Translation: AAF29404.1.
    CP002684 Genomic DNA. Translation: AEE32766.1.
    AY084631 mRNA. Translation: AAM61194.1.
    AY058204 mRNA. Translation: AAL25616.1. Different initiation.
    AY098981 mRNA. Translation: AAM19991.1.
    PIRiH96561.
    RefSeqiNP_175630.1. NM_104099.3.
    UniGeneiAt.25542.

    Genome annotation databases

    EnsemblPlantsiAT1G52190.1; AT1G52190.1; AT1G52190.
    GeneIDi841649.
    GrameneiAT1G52190.1; AT1G52190.1; AT1G52190.
    KEGGiath:AT1G52190.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC022354 Genomic DNA. Translation: AAF29404.1.
    CP002684 Genomic DNA. Translation: AEE32766.1.
    AY084631 mRNA. Translation: AAM61194.1.
    AY058204 mRNA. Translation: AAL25616.1. Different initiation.
    AY098981 mRNA. Translation: AAM19991.1.
    PIRiH96561.
    RefSeqiNP_175630.1. NM_104099.3.
    UniGeneiAt.25542.

    3D structure databases

    ProteinModelPortaliQ9M817.
    SMRiQ9M817. Positions 19-564.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi26874. 4 interactions.
    STRINGi3702.AT1G52190.1.

    Protein family/group databases

    TCDBi2.A.17.3.13. the proton-dependent oligopeptide transporter (pot/ptr) family.

    PTM databases

    iPTMnetiQ9M817.

    Proteomic databases

    PaxDbiQ9M817.
    PRIDEiQ9M817.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G52190.1; AT1G52190.1; AT1G52190.
    GeneIDi841649.
    GrameneiAT1G52190.1; AT1G52190.1; AT1G52190.
    KEGGiath:AT1G52190.

    Organism-specific databases

    TAIRiAT1G52190.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237399.
    InParanoidiQ9M817.
    OMAiGGKESWV.
    OrthoDBiEOG093605FJ.
    PhylomeDBiQ9M817.

    Enzyme and pathway databases

    ReactomeiR-ATH-427975. Proton/oligopeptide cotransporters.

    Miscellaneous databases

    PROiQ9M817.

    Gene expression databases

    GenevisibleiQ9M817. AT.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 1 hit.
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPTR6_ARATH
    AccessioniPrimary (citable) accession number: Q9M817
    Secondary accession number(s): Q8LFV0, Q93Z23
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: October 1, 2000
    Last modified: September 7, 2016
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.