Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proliferating cellular nuclear antigen 1

Gene

PCNA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi61 – 8020Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-110312. Translesion synthesis by REV1.
R-ATH-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-ATH-110320. Translesion Synthesis by POLH.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-174411. Polymerase switching on the C-strand of the telomere.
R-ATH-174414. Processive synthesis on the C-strand of the telomere.
R-ATH-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-ATH-174437. Removal of the Flap Intermediate from the C-strand.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-ATH-5655862. Translesion synthesis by POLK.
R-ATH-5656121. Translesion synthesis by POLI.
R-ATH-5656169. Termination of translesion DNA synthesis.
R-ATH-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-69091. Polymerase switching.
R-ATH-69109. Leading Strand Synthesis.
R-ATH-69166. Removal of the Flap Intermediate.
R-ATH-69183. Processive synthesis on the lagging strand.
R-ATH-69205. G1/S-Specific Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferating cellular nuclear antigen 1
Short name:
PCNA 1
Gene namesi
Name:PCNA
Synonyms:PCNA1
Ordered Locus Names:At1g07370
ORF Names:F22G5.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G07370.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • PCNA complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Proliferating cellular nuclear antigen 1PRO_0000149177Add
BLAST

Proteomic databases

PaxDbiQ9M7Q7.
PRIDEiQ9M7Q7.

Expressioni

Gene expression databases

GenevisibleiQ9M7Q7. AT.

Interactioni

Subunit structurei

Homo- and heterotrimer. Interacts with POLH, ATXR5 and ATXR6.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
POLHQ8H2D52EBI-1810458,EBI-1810451

Protein-protein interaction databases

BioGridi22490. 17 interactions.
IntActiQ9M7Q7. 7 interactions.
STRINGi3702.AT1G07370.1.

Structurei

Secondary structure

1
263
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi10 – 1910Combined sources
Turni20 – 223Combined sources
Beta strandi24 – 318Combined sources
Beta strandi34 – 407Combined sources
Beta strandi44 – 5310Combined sources
Helixi54 – 563Combined sources
Beta strandi57 – 648Combined sources
Beta strandi66 – 716Combined sources
Helixi72 – 809Combined sources
Beta strandi87 – 926Combined sources
Beta strandi97 – 1048Combined sources
Beta strandi111 – 1177Combined sources
Beta strandi134 – 1407Combined sources
Helixi141 – 15212Combined sources
Beta strandi156 – 1638Combined sources
Beta strandi166 – 1738Combined sources
Beta strandi176 – 1827Combined sources
Helixi191 – 1933Combined sources
Beta strandi196 – 2016Combined sources
Beta strandi203 – 2086Combined sources
Helixi209 – 2157Combined sources
Helixi216 – 2216Combined sources
Beta strandi223 – 2297Combined sources
Beta strandi235 – 2417Combined sources
Turni242 – 2443Combined sources
Beta strandi245 – 2517Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZVVX-ray2.00A/B1-256[»]
ProteinModelPortaliQ9M7Q7.
SMRiQ9M7Q7. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9M7Q7.

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.Curated

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
HOGENOMiHOG000211098.
InParanoidiQ9M7Q7.
KOiK04802.
OMAiANVGMVD.
PhylomeDBiQ9M7Q7.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M7Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELRLVQGS LLKKVLESIK DLVNDANFDC SSTGFSLQAM DSSHVALVSL
60 70 80 90 100
LLRSEGFEHY RCDRNLSMGM NLGNMSKMLK CAGNDDIITI KADDGGDTVT
110 120 130 140 150
FMFESPTQDK IADFEMKLMD IDSEHLGIPD AEYHSIVRMP SNEFSRICKD
160 170 180 190 200
LSSIGDTVVI SVTKEGVKFS TAGDIGTANI VLRQNTTVDK PEDAIVIEMK
210 220 230 240 250
EPVSLSFALR YMNSFTKATP LSDTVTISLS SELPVVVEYK VAEMGYIRYY
260
LAPKIEEEED TNP
Length:263
Mass (Da):29,122
Last modified:February 21, 2001 - v2
Checksum:iDA63C0F637CDB5D7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481V → A in AAF40018 (Ref. 1) Curated
Sequence conflicti48 – 481V → A in ABU25233 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083220 mRNA. Translation: AAF40018.1.
EU072917 mRNA. Translation: ABU25233.1.
AC022464 Genomic DNA. Translation: AAF79566.1.
CP002684 Genomic DNA. Translation: AEE28115.1.
AF462821 mRNA. Translation: AAL58911.1.
AY098969 mRNA. Translation: AAM19979.1.
AY086852 mRNA. Translation: AAM63900.1.
RefSeqiNP_172217.1. NM_100611.3.
UniGeneiAt.11997.

Genome annotation databases

EnsemblPlantsiAT1G07370.1; AT1G07370.1; AT1G07370.
GeneIDi837249.
GrameneiAT1G07370.1; AT1G07370.1; AT1G07370.
KEGGiath:AT1G07370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083220 mRNA. Translation: AAF40018.1.
EU072917 mRNA. Translation: ABU25233.1.
AC022464 Genomic DNA. Translation: AAF79566.1.
CP002684 Genomic DNA. Translation: AEE28115.1.
AF462821 mRNA. Translation: AAL58911.1.
AY098969 mRNA. Translation: AAM19979.1.
AY086852 mRNA. Translation: AAM63900.1.
RefSeqiNP_172217.1. NM_100611.3.
UniGeneiAt.11997.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZVVX-ray2.00A/B1-256[»]
ProteinModelPortaliQ9M7Q7.
SMRiQ9M7Q7. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22490. 17 interactions.
IntActiQ9M7Q7. 7 interactions.
STRINGi3702.AT1G07370.1.

Proteomic databases

PaxDbiQ9M7Q7.
PRIDEiQ9M7Q7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G07370.1; AT1G07370.1; AT1G07370.
GeneIDi837249.
GrameneiAT1G07370.1; AT1G07370.1; AT1G07370.
KEGGiath:AT1G07370.

Organism-specific databases

TAIRiAT1G07370.

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
HOGENOMiHOG000211098.
InParanoidiQ9M7Q7.
KOiK04802.
OMAiANVGMVD.
PhylomeDBiQ9M7Q7.

Enzyme and pathway databases

ReactomeiR-ATH-110312. Translesion synthesis by REV1.
R-ATH-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-ATH-110320. Translesion Synthesis by POLH.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-174411. Polymerase switching on the C-strand of the telomere.
R-ATH-174414. Processive synthesis on the C-strand of the telomere.
R-ATH-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-ATH-174437. Removal of the Flap Intermediate from the C-strand.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-ATH-5655862. Translesion synthesis by POLK.
R-ATH-5656121. Translesion synthesis by POLI.
R-ATH-5656169. Termination of translesion DNA synthesis.
R-ATH-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-69091. Polymerase switching.
R-ATH-69109. Leading Strand Synthesis.
R-ATH-69166. Removal of the Flap Intermediate.
R-ATH-69183. Processive synthesis on the lagging strand.
R-ATH-69205. G1/S-Specific Transcription.

Miscellaneous databases

EvolutionaryTraceiQ9M7Q7.
PROiQ9M7Q7.

Gene expression databases

GenevisibleiQ9M7Q7. AT.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of Arabidopsis proliferating cellular nuclear antigen (PCNA)."
    Park S.C., Park E.H., Cho J.W.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Di Rubbo A., Vonarx E.J., Kunz B.A.
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Two cell-cycle regulated SET-domain proteins interact with proliferating cell nuclear antigen (PCNA) in Arabidopsis."
    Raynaud C., Sozzani R., Glab N., Domenichini S., Perennes C., Cella R., Kondorosi E., Bergounioux C.
    Plant J. 47:395-407(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATXR5 AND ATXR6.
  8. "Arabidopsis thaliana Y-family DNA polymerase eta catalyses translesion synthesis and interacts functionally with PCNA2."
    Anderson H.J., Vonarx E.J., Pastushok L., Nakagawa M., Katafuchi A., Gruz P., Di Rubbo A., Grice D.M., Osmond M.J., Sakamoto A.N., Nohmi T., Xiao W., Kunz B.A.
    Plant J. 55:895-908(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POLH.
  9. "Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment."
    Strzalka W., Oyama T., Tori K., Morikawa K.
    Protein Sci. 18:1072-1080(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 1-256 IN COMPLEX WITH HUMAN P21 PEPTIDE, SUBUNIT.

Entry informationi

Entry nameiPCNA1_ARATH
AccessioniPrimary (citable) accession number: Q9M7Q7
Secondary accession number(s): A7UIK5, Q9LNV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: February 17, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.