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Protein

Proliferating cellular nuclear antigen 1

Gene

PCNA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi61 – 80Sequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-110312. Translesion synthesis by REV1.
R-ATH-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-ATH-110320. Translesion Synthesis by POLH.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-174411. Polymerase switching on the C-strand of the telomere.
R-ATH-174414. Processive synthesis on the C-strand of the telomere.
R-ATH-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-ATH-174437. Removal of the Flap Intermediate from the C-strand.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-ATH-5655862. Translesion synthesis by POLK.
R-ATH-5656121. Translesion synthesis by POLI.
R-ATH-5656169. Termination of translesion DNA synthesis.
R-ATH-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-69091. Polymerase switching.
R-ATH-69166. Removal of the Flap Intermediate.
R-ATH-69183. Processive synthesis on the lagging strand.
R-ATH-69205. G1/S-Specific Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Proliferating cellular nuclear antigen 1
Short name:
PCNA 1
Gene namesi
Name:PCNA
Synonyms:PCNA1
Ordered Locus Names:At1g07370
ORF Names:F22G5.29
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G07370.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • PCNA complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001491771 – 263Proliferating cellular nuclear antigen 1Add BLAST263

Proteomic databases

PaxDbiQ9M7Q7.
PRIDEiQ9M7Q7.

PTM databases

iPTMnetiQ9M7Q7.

Expressioni

Gene expression databases

GenevisibleiQ9M7Q7. AT.

Interactioni

Subunit structurei

Homo- and heterotrimer. Interacts with POLH, ATXR5 and ATXR6.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
POLHQ8H2D52EBI-1810458,EBI-1810451

Protein-protein interaction databases

BioGridi22490. 18 interactors.
IntActiQ9M7Q7. 7 interactors.
STRINGi3702.AT1G07370.1.

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi10 – 19Combined sources10
Turni20 – 22Combined sources3
Beta strandi24 – 31Combined sources8
Beta strandi34 – 40Combined sources7
Beta strandi44 – 53Combined sources10
Helixi54 – 56Combined sources3
Beta strandi57 – 64Combined sources8
Beta strandi66 – 71Combined sources6
Helixi72 – 80Combined sources9
Beta strandi87 – 92Combined sources6
Beta strandi97 – 104Combined sources8
Beta strandi111 – 117Combined sources7
Beta strandi134 – 140Combined sources7
Helixi141 – 152Combined sources12
Beta strandi156 – 163Combined sources8
Beta strandi166 – 173Combined sources8
Beta strandi176 – 182Combined sources7
Helixi191 – 193Combined sources3
Beta strandi196 – 201Combined sources6
Beta strandi203 – 208Combined sources6
Helixi209 – 215Combined sources7
Helixi216 – 221Combined sources6
Beta strandi223 – 229Combined sources7
Beta strandi235 – 241Combined sources7
Turni242 – 244Combined sources3
Beta strandi245 – 251Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZVVX-ray2.00A/B1-256[»]
ProteinModelPortaliQ9M7Q7.
SMRiQ9M7Q7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9M7Q7.

Family & Domainsi

Sequence similaritiesi

Belongs to the PCNA family.Curated

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
HOGENOMiHOG000211098.
InParanoidiQ9M7Q7.
KOiK04802.
OMAiANVGMVD.
OrthoDBiEOG09360IU7.
PhylomeDBiQ9M7Q7.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9M7Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLELRLVQGS LLKKVLESIK DLVNDANFDC SSTGFSLQAM DSSHVALVSL
60 70 80 90 100
LLRSEGFEHY RCDRNLSMGM NLGNMSKMLK CAGNDDIITI KADDGGDTVT
110 120 130 140 150
FMFESPTQDK IADFEMKLMD IDSEHLGIPD AEYHSIVRMP SNEFSRICKD
160 170 180 190 200
LSSIGDTVVI SVTKEGVKFS TAGDIGTANI VLRQNTTVDK PEDAIVIEMK
210 220 230 240 250
EPVSLSFALR YMNSFTKATP LSDTVTISLS SELPVVVEYK VAEMGYIRYY
260
LAPKIEEEED TNP
Length:263
Mass (Da):29,122
Last modified:February 21, 2001 - v2
Checksum:iDA63C0F637CDB5D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48V → A in AAF40018 (Ref. 1) Curated1
Sequence conflicti48V → A in ABU25233 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083220 mRNA. Translation: AAF40018.1.
EU072917 mRNA. Translation: ABU25233.1.
AC022464 Genomic DNA. Translation: AAF79566.1.
CP002684 Genomic DNA. Translation: AEE28115.1.
AF462821 mRNA. Translation: AAL58911.1.
AY098969 mRNA. Translation: AAM19979.1.
AY086852 mRNA. Translation: AAM63900.1.
RefSeqiNP_172217.1. NM_100611.4.
UniGeneiAt.11997.

Genome annotation databases

EnsemblPlantsiAT1G07370.1; AT1G07370.1; AT1G07370.
GeneIDi837249.
GrameneiAT1G07370.1; AT1G07370.1; AT1G07370.
KEGGiath:AT1G07370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083220 mRNA. Translation: AAF40018.1.
EU072917 mRNA. Translation: ABU25233.1.
AC022464 Genomic DNA. Translation: AAF79566.1.
CP002684 Genomic DNA. Translation: AEE28115.1.
AF462821 mRNA. Translation: AAL58911.1.
AY098969 mRNA. Translation: AAM19979.1.
AY086852 mRNA. Translation: AAM63900.1.
RefSeqiNP_172217.1. NM_100611.4.
UniGeneiAt.11997.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZVVX-ray2.00A/B1-256[»]
ProteinModelPortaliQ9M7Q7.
SMRiQ9M7Q7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22490. 18 interactors.
IntActiQ9M7Q7. 7 interactors.
STRINGi3702.AT1G07370.1.

PTM databases

iPTMnetiQ9M7Q7.

Proteomic databases

PaxDbiQ9M7Q7.
PRIDEiQ9M7Q7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G07370.1; AT1G07370.1; AT1G07370.
GeneIDi837249.
GrameneiAT1G07370.1; AT1G07370.1; AT1G07370.
KEGGiath:AT1G07370.

Organism-specific databases

TAIRiAT1G07370.

Phylogenomic databases

eggNOGiKOG1636. Eukaryota.
COG0592. LUCA.
HOGENOMiHOG000211098.
InParanoidiQ9M7Q7.
KOiK04802.
OMAiANVGMVD.
OrthoDBiEOG09360IU7.
PhylomeDBiQ9M7Q7.

Enzyme and pathway databases

ReactomeiR-ATH-110312. Translesion synthesis by REV1.
R-ATH-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-ATH-110320. Translesion Synthesis by POLH.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-174411. Polymerase switching on the C-strand of the telomere.
R-ATH-174414. Processive synthesis on the C-strand of the telomere.
R-ATH-174417. Telomere C-strand (Lagging Strand) Synthesis.
R-ATH-174437. Removal of the Flap Intermediate from the C-strand.
R-ATH-4615885. SUMOylation of DNA replication proteins.
R-ATH-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
R-ATH-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-ATH-5655862. Translesion synthesis by POLK.
R-ATH-5656121. Translesion synthesis by POLI.
R-ATH-5656169. Termination of translesion DNA synthesis.
R-ATH-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER.
R-ATH-5696400. Dual Incision in GG-NER.
R-ATH-6782135. Dual incision in TC-NER.
R-ATH-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-ATH-69091. Polymerase switching.
R-ATH-69166. Removal of the Flap Intermediate.
R-ATH-69183. Processive synthesis on the lagging strand.
R-ATH-69205. G1/S-Specific Transcription.

Miscellaneous databases

EvolutionaryTraceiQ9M7Q7.
PROiQ9M7Q7.

Gene expression databases

GenevisibleiQ9M7Q7. AT.

Family and domain databases

HAMAPiMF_00317. DNApol_clamp_arch. 1 hit.
InterProiIPR000730. Pr_cel_nuc_antig.
IPR022649. Pr_cel_nuc_antig_C.
IPR022659. Pr_cel_nuc_antig_CS.
IPR022648. Pr_cel_nuc_antig_N.
[Graphical view]
PANTHERiPTHR11352. PTHR11352. 1 hit.
PfamiPF02747. PCNA_C. 1 hit.
PF00705. PCNA_N. 1 hit.
[Graphical view]
PRINTSiPR00339. PCNACYCLIN.
TIGRFAMsiTIGR00590. pcna. 1 hit.
PROSITEiPS01251. PCNA_1. 1 hit.
PS00293. PCNA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCNA1_ARATH
AccessioniPrimary (citable) accession number: Q9M7Q7
Secondary accession number(s): A7UIK5, Q9LNV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 30, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.