ABSCISIC ACID-INSENSITIVE 5-like protein 7 - Arabidopsis thaliana (Mouse-ear cress)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q9M7Q2 (AI5L7_ARATH)

Last modified June 16, 2009. Version 50. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    ABSCISIC ACID-INSENSITIVE 5-like protein 7
Alternative name(s):
    Abscisic acid responsive elements-binding factor 4
      Short name=ABRE-binding factor 4
    ABA-responsive element-binding protein 2
    bZIP transcription factor 38
      Short name=AtbZIP38

Gene names

Name:	ABF4
Synonyms:	AREB2, BZIP38
Ordered Locus Names:	At3g19290
ORF Names:	MVI11.7

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Functions as transcriptional activator in the ABA-inducible expression of rd29B. Binds specifically to the ABA-responsive element (ABRE) of the rd29B gene promoter. Ref.2 Ref.5 Ref.7 Ref.10
Subunit structure	DNA-binding heterodimer By similarity. Interacts with CPK32 and the AFP proteins AFP1, AFP2 and AFP3.
Subcellular location	Nucleus By similarity.
Tissue specificity	Expressed in roots, leaves, flowers and immatures siliques. Ref.2 Ref.5
Induction	Up-regulated by drought, salt, abscisic acid (ABA) and cold. Ref.2 Ref.10 Ref.1 Ref.8
Post-translational modification	Phosphorylated by CPK4 and CPK11 in vitro. Ref.9 Ref.12
Disruption phenotype	Defective in ABA and stress responses. Ref.7
Sequence similarities	Belongs to the bZIP family. ABI5 subfamily. Contains 1 bZIP domain.
Sequence caution	The sequence BAB02453.1 differs from that shown. Reason: Erroneous gene model prediction.

Hide | Top

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	abscisic acid mediated signaling Ref.5 Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to salt stress Ref.10 Inferred from expression pattern. Source: TAIR response to water deprivation Ref.10 Inferred from expression pattern. Source: TAIR transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.9 Inferred from direct assay. Source: TAIR
Molecular function	protein dimerization activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription activator activity Ref.2 Inferred from direct assay. Source: TAIR transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Hide | Top

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q2V412	1	EBI-1237867,EBI-1238781
CALM	P62157	1	EBI-1237867,EBI-397403	From a different organism.
CAM1	P25854	1	EBI-1237867,EBI-1235664
CAM6	Q03509	1	EBI-1237867,EBI-1236097
CAM7	P59220	1	EBI-1237867,EBI-1236031
CML10	P30187	1	EBI-1237867,EBI-1238722
CML8	O23320	1	EBI-1237867,EBI-1237764
CML9	Q9S744	1	EBI-1237867,EBI-1236048
CPK32	Q6NLQ6	4	EBI-1237867,EBI-1538032

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 431

431

ABSCISIC ACID-INSENSITIVE 5-like protein 7

PRO_0000369612

Regions

Domain

379 – 393

Leucine-zipper

DNA binding

353 – 372

Basic motif

Compositional bias

184 – 189

Poly-Asn

Compositional bias

239 – 246

Poly-Gln

Compositional bias

249 – 257

Poly-Gln

Amino acid modifications

Modified residue

Phosphoserine Potential

Modified residue

110

Phosphoserine; by CPK32 Ref.9

Modified residue

155

Phosphothreonine Potential

Experimental info

Mutagenesis

S → A: Abolishes ABA-dependent phosphorylation. Ref.11

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q9M7Q2-1 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 562FB52A1B493AF3
Show »

FASTA

431

46,492

        10         20         30         40         50         60 
MGTHINFNNL GGGGHPGGEG SSNQMKPTGS VMPLARQSSV YSLTFDELQN TLGGPGKDFG 

        70         80         90        100        110        120 
SMNMDELLKS IWTAEEAQAM AMTSAPAATA VAQPGAGIPP PGGNLQRQGS LTLPRTISQK 

       130        140        150        160        170        180 
TVDEVWKCLI TKDGNMEGSS GGGGESNVPP GRQQTLGEMT LEEFLFRAGV VREDNCVQQM 

       190        200        210        220        230        240 
GQVNGNNNNG FYGNSTAAGG LGFGFGQPNQ NSITFNGTND SMILNQPPGL GLKMGGTMQQ 

       250        260        270        280        290        300 
QQQQQQLLQQ QQQQMQQLNQ PHPQQRLPQT IFPKQANVAF SAPVNITNKG FAGAANNSIN 

       310        320        330        340        350        360 
NNNGLASYGG TGVTVAATSP GTSSAENNSL SPVPYVLNRG RRSNTGLEKV IERRQRRMIK 

       370        380        390        400        410        420 
NRESAARSRA RKQAYTLELE AEIEKLKKTN QELQKKQAEM VEMQKNELKE TSKRPWGSKR 

       430 
QCLRRTLTGP W

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ABFs, a family of ABA-responsive element binding factors." Choi H.-I., Hong J.-H., Ha J.-O., Kang J.-Y., Kim S.Y. J. Biol. Chem. 275:1723-1730(2000) [PubMed: 10636868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DNA-BINDING, INDUCTION. Strain: cv. Columbia. Tissue: Seedling.
[2]	"Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions." Uno Y., Furihata T., Abe H., Yoshida R., Shinozaki K., Yamaguchi-Shinozaki K. Proc. Natl. Acad. Sci. U.S.A. 97:11632-11637(2000) [PubMed: 11005831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling." Kang J.-Y., Choi H.-I., Im M.-Y., Kim S.Y. Plant Cell 14:343-357(2002) [PubMed: 11884679] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"bZIP transcription factors in Arabidopsis." Jakoby M., Weisshaar B., Droege-Laser W., Vicente-Carbajosa J., Tiedemann J., Kroj T., Parcy F. Trends Plant Sci. 7:106-111(2002) [PubMed: 11906833] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"ABF2, an ABRE-binding bZIP factor, is an essential component of glucose signaling and its overexpression affects multiple stress tolerance." Kim S., Kang J.-Y., Cho D.-I., Park J.H., Kim S.Y. Plant J. 40:75-87(2004) [PubMed: 15361142] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[8]	"AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis." Fujita Y., Fujita M., Satoh R., Maruyama K., Parvez M.M., Seki M., Hiratsu K., Ohme-Takagi M., Shinozaki K., Yamaguchi-Shinozaki K. Plant Cell 17:3470-3488(2005) [PubMed: 16284313] [Abstract] Cited for: INDUCTION.
[9]	"Arabidopsis calcium-dependent protein kinase AtCPK32 interacts with ABF4, a transcriptional regulator of abscisic acid-responsive gene expression, and modulates its activity." Choi H.-I., Park H.-J., Park J.H., Kim S., Im M.-Y., Seo H.-H., Kim Y.-W., Hwang I., Kim S.Y. Plant Physiol. 139:1750-1761(2005) [PubMed: 16299177] [Abstract] Cited for: INTERACTION WITH CPK32, PHOSPHORYLATION AT SER-110 BY CPK32.
[10]	"Transcriptional regulation of ABI3- and ABA-responsive genes including RD29B and RD29A in seeds, germinating embryos, and seedlings of Arabidopsis." Nakashima K., Fujita Y., Katsura K., Maruyama K., Narusaka Y., Seki M., Shinozaki K., Yamaguchi-Shinozaki K. Plant Mol. Biol. 60:51-68(2006) [PubMed: 16463099] [Abstract] Cited for: FUNCTION, INDUCTION.
[11]	"Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1." Furihata T., Maruyama K., Fujita Y., Umezawa T., Yoshida R., Shinozaki K., Yamaguchi-Shinozaki K. Proc. Natl. Acad. Sci. U.S.A. 103:1988-1993(2006) [PubMed: 16446457] [Abstract] Cited for: MUTAGENESIS OF SER-39.
[12]	"Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis." Zhu S.-Y., Yu X.-C., Wang X.-J., Zhao R., Li Y., Fan R.-C., Shang Y., Du S.-Y., Wang X.-F., Wu F.-Q., Xu Y.-H., Zhang X.-Y., Zhang D.-P. Plant Cell 19:3019-3036(2007) [PubMed: 17921317] [Abstract] Cited for: PHOSPHORYLATION BY CPK4 AND CPK11.
[13]	"A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings." Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R. Plant Mol. Biol. 67:643-658(2008) [PubMed: 18484180] [Abstract] Cited for: INTERACTION WITH AFP1; AFP2 AND AFP3.

Hide | Top

Cross-references

Sequence databases
	AF093547 mRNA. Translation: AAF27182.1. AB017161 mRNA. Translation: BAB12405.1. AB025624 Genomic DNA. Translation: BAB02453.1. Sequence problems. AY050897 mRNA. Translation: AAK92834.1. AY091298 mRNA. Translation: AAM14237.1.
IPI	IPI00525502.
RefSeq	NP_566629.1.
UniGene	At.23993
3D structure databases
HSSP	HSSP built from PDB template 1JNM based on UniProtKB P05412.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9M7Q2. 15 interactions.
Genome annotation databases
GeneID	821463.
GenomeReviews	Gene locus AT3G19290 in contig BA000014_GR.
KEGG	ath:AT3G19290.
NMPDR	fig\|3702.1.peg.14142.
Organism-specific databases
TAIR	At3g19290.
Phylogenomic databases
OMA	Q9M7Q2. ASHISSD.
Gene expression databases
ArrayExpress	Q9M7Q2.
Family and domain databases
InterPro	IPR011616. bZIP_1. IPR004827. TF_bZIP. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

AI5L7_ARATH

Accession

Primary (citable) accession number: Q9M7Q2
Secondary accession number(s): Q9LT88

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents