Reviewed, UniProtKB/Swiss-Prot Q9M5Q1 (FUT1_PEA)

Names and origin

Protein names

Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase
    EC=2.4.1.69
Alternative name(s):
    Xyloglucan alpha-(1,2)-fucosyltransferase
    PsFT1

Gene names

Name:

FT1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in cell wall biosynthesis. Adds the terminal fucosyl residue on xyloglucan side chains. Ref.1
Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Sequence similarities	Belongs to the glycosyltransferase 37 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell wall biogenesis Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Galactoside 2-alpha-L-fucosyltransferase		PRO_0000193910
Regions
	Topological domain	1 – 43	43	Cytoplasmic Potential
	Transmembrane	44 – 64	21	Signal-anchor for type II membrane protein Potential
	Topological domain	65 – 565	501	Lumenal Potential
Amino acid modifications
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Glycosylation	263	1	N-linked (GlcNAc...) Potential
	Glycosylation	407	1	N-linked (GlcNAc...) Potential
	Glycosylation	509	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9M5Q1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5E6AAA2BED20594B	FASTA	565	64,029
	10 20 30 40 50 60 MNMLIKRVIA IKNPRGDDNN NNKLSDLETL TDKCTTCPLT LMRVMAFFVV SFMLFSVLFS 70 80 90 100 110 120 LSVVLRDPPS DAAISSTTTL FQLNQGLGSD DFDSVELLND KLLGGLLADG FDEKSCLSRY 130 140 150 160 170 180 QSAIFGKGLS GKPSSYLISR LRKYEARHKQ CGPYTESYNK TVKELGSGQF SESVDCKYVV 190 200 210 220 230 240 WISFSGLGNR ILTLVSAFLY ALLTDRVLLV DPGVDMTDLF CEPFPDASWF VPPDFPLNSH 250 260 270 280 290 300 LNNFNQESNQ CHGKILKTKS ITNSTVPSFV YLHLAHDYDD HDKLFFCDEE QLFLQNVPLL 310 320 330 340 350 360 IMKTDNYFIP SLFLMPSFEQ ELNDLFPKKE KVFHFLGRYL LHPTNNVWGL VVRYYDAYLA 370 380 390 400 410 420 KVDERIGIQI RVFDTDPGPF QHVLDQVLAC TLKESILPDV NREQNINSSS GTPKSKAVLI 430 440 450 460 470 480 TSLSSGYFEK VRDMYWEFPT ETGEVVGIYQ PSHEGYQQTQ KQFHNQKAWA EMYLLSLTDV 490 500 510 520 530 540 LVTSSWSTFG YVAQGLGGLK PWILYKPENR TAPNPPCQRA MSMEPCFHAP PFYDCKAKRG 550 560 TDTGALVPHV RHCEDMSWGL KLVDN

References

[1]

"Biochemical characterization and molecular cloning of an alpha-1,2-fucosyltransferase that catalyzes the last step of cell wall xyloglucan biosynthesis in Pea." Faik A., Bar-Peled M., DeRocher A.E., Zeng W., Perrin R.M., Wilkerson C., Raikhel N.V., Keegstra K. J. Biol. Chem. 275:15082-15089(2000) [PubMed: 10747946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: cv. Alaska.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF223643 mRNA. Translation: AAF62896.1.
3D structure databases
SMR	Q9M5Q1. Positions 176-498.
ModBase	Search...
Protein family/group databases
CAZy	GT37. Glycosyltransferase Family 37.
Enzyme and pathway databases
BRENDA	2.4.1.69. 287.
Family and domain databases
InterPro	IPR004938. XG_FTase. [Graphical view]
Pfam	PF03254. XG_FTase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT1_PEA

Accession

Primary (citable) accession number: Q9M5Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)