Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic precursor

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Q9M5P4 (CRS2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 54. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic

Alternative name(s):
Chloroplastic RNA splicing factor 2
Protein CHLOROPLAST RNA SPLICING 2

Gene names

Name:

CRS2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the splicing of group IIB introns in chloroplasts. Forms complexes with either CAF1 or CAF2 which, in turn, interact with RNA and confer intron specificity of the splicing particles. Has no peptidyl-tRNA hydrolase activity. Ref.1 Ref.2 Ref.3 Ref.4
Subunit structure	Interacts with CAF1 and CAF2. Part of large ribonucleo-protein complexes that include group IIB introns and either CAF1 or CAF2. Ref.1 Ref.4 Ref.5 Ref.6
Subcellular location	Plastid › chloroplast stroma Ref.1.
Sequence similarities	Belongs to the PTH family. CRS2 subfamily.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Ribonucleoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 45

Chloroplast Potential

Chain

46 – 256

211

Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic

PRO_0000280532

Experimental info

Mutagenesis

100

I → N: Reduced interaction with CAF1 and CAF2. Loss of interaction with CAF1 and CAF2; when associated with F-102. Loss of interaction with CAF1 and CAF2; when associated with T-108. Ref.6

Mutagenesis

102

S → F: Loss of interaction with CAF1 and CAF2; when associated with N-100. Ref.6

Mutagenesis

108

I → T: Loss of interaction with CAF1 and CAF2; when associated with N-100. Ref.6

Secondary structure

256

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9M5P4 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 11D15227394545CB
Show »

FASTA

256

28,214

        10         20         30         40         50         60 
MSLLAAAIPS TSSHFSAPFL PSFRMPRKSL TAPLHRIRRP RPFTVVSSVP DPAAGPVEYT 

        70         80         90        100        110        120 
PWLIAGLGNP GNKYYGTRHN VGFEMVDRIA AEEGITMNTI QSKSLLGIGS IGEVPVLVVK 

       130        140        150        160        170        180 
PQSYMNYSGE AIGPLAAYYQ VPLRHILLIY DDTSLPNGVL RLQKKGGHGR HNGLQNVIEH 

       190        200        210        220        230        240 
LDGRREFPRL SIGIGSPPGK MDPRAFLLQK FSSEERVQID TALEQGVDAV RTLVLKGFSG 

       250 
STERFNLVQK YKFHRV

« Hide

References

[1]	"Recruitment of a peptidyl-tRNA hydrolase as a facilitator of group II intron splicing in chloroplasts." Jenkins B.D., Barkan A. EMBO J. 20:872-879(2001) [PubMed: 11179231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RNA, SUBCELLULAR LOCATION.
[2]	"Nuclear mutations that block group II RNA splicing in maize chloroplasts reveal several intron classes with distinct requirements for splicing factors." Jenkins B.D., Kulhanek D.J., Barkan A. Plant Cell 9:283-296(1997) [PubMed: 9090875] [Abstract] Cited for: FUNCTION.
[3]	"Comparative analysis of splicing of the complete set of chloroplast group II introns in three higher plant mutants." Vogel J., Boerner T., Hess W.R. Nucleic Acids Res. 27:3866-3874(1999) [PubMed: 10481026] [Abstract] Cited for: FUNCTION.
[4]	"Group II intron splicing factors derived by diversification of an ancient RNA-binding domain." Ostheimer G.J., Williams-Carrier R., Belcher S., Osborne E., Gierke J., Barkan A. EMBO J. 22:3919-3929(2003) [PubMed: 12881426] [Abstract] Cited for: FUNCTION, INTERACTION WITH CAF1 AND CAF2.
[5]	"Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2." Ostheimer G.J., Rojas M., Hadjivassiliou H., Barkan A. J. Biol. Chem. 281:4732-4738(2006) [PubMed: 16379013] [Abstract] Cited for: INTERACTION WITH CAF1 AND CAF2.
[6]	"Structural analysis of the group II intron splicing factor CRS2 yields insights into its protein and RNA interaction surfaces." Ostheimer G.J., Hadjivassiliou H., Kloer D.P., Barkan A., Matthews B.W. J. Mol. Biol. 345:51-68(2005) [PubMed: 15567410] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 55-256, INTERACTION WITH CAF1 AND CAF2, MUTAGENESIS OF ILE-100; SER-102 AND ILE-108.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF225708 mRNA. Translation: AAF27939.1.

RefSeq

NP_001104963.1. NM_001111493.1.

UniGene

Zm.478.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RYB	X-ray	1.70	A	57-256	[»]
1RYM	X-ray	1.80	A	57-256	[»]
1RYN	X-ray	1.75	A	58-250	[»]

ProteinModelPortal

Q9M5P4.

SMR

Q9M5P4. Positions 58-254.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

541812.

KEGG

zma:541812.

Organism-specific databases

Gramene

Q9M5P4.

MaizeGDB

106358.

Phylogenomic databases

GeneTree

EPGT00050000018764.

Family and domain databases

InterPro

IPR001328. Pept_tRNA_hydro.
IPR018171. Pept_tRNA_hydro_CS.
[Graphical view]

Gene3D

G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.

K01056.

PANTHER

PTHR17224. Pept_tRNA_hydro. 1 hit.

Pfam

PF01195. Pept_tRNA_hydro. 1 hit.
[Graphical view]

SUPFAM

SSF53178. Pept_tRNA_hydro. 1 hit.

TIGRFAMs

TIGR00447. Pth. 1 hit.

PROSITE

PS01195. PEPT_TRNA_HYDROL_1. 1 hit.
PS01196. PEPT_TRNA_HYDROL_2. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CRS2_MAIZE

Accession

Primary (citable) accession number: Q9M5P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents